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Gene information
General information
TC002132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19901900..19910612
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002132
    iBeetle screen iB_00332

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002132 RA
      MSVVTYEKP IQHIGLADW HAKQWQNQQ TNDTRRTDA 
FNLRHESRQ LRNETNIRT EWDTYHNNV RLADRITEL 
DRWRDVLTC CLERVDKEI LSLKDEKFS TERELDALG 
IPLGVVAEC ISMRDCRQG AELTYDDGD TELKRELCV 
VEGVKKLLI ERCQAAWEK LNKLEEVRF KLNLELNDK 
TEAIEIDKD QLTLDKNCA NISFKTDPL RIVKNSIPY 
EAWLEHSRF IKELADNEL ADTHKLREA LFVVRERAR 
NDLRAQRDR VDFTLRKRI YETQKARNE LEWQQFKMR 
EEMDKLLKE IKTLEDALL AKTDALKLC ETRLENRAY 
RPGFELARD ETETGLKNE VTQLRQTRN DLINKINCA 
KATYNALEN QQVIIDTDL ENKSHSLMT DIRCLDMRI 
RLRTGEFAG PGTDTDRNI QLTRMEQEI PPT
    CDS sequence
    • >TC002132 RA
      atgtcagtg gtaacgtac gaaaaaccc attcaacac 
attggatta gccgactgg cacgccaaa cagtggcaa 
aaccaacaa accaatgac acccgaaga acggacgcc 
tttaattta cgacacgag tcgagacaa ttacgaaat 
gagactaat attcggacc gaatgggac acttaccac 
aacaatgtg cgtctagct gatcgcatt acagagctt 
gataggtgg agagatgtt ctaacttgt tgtttagaa 
cgagtcgat aaagaaatc ctctctcta aaagacgag 
aaattttcc accgaaagg gaacttgat gctcttgga 
atacctctc ggagttgtt gccgagtgc atttcgatg 
agagactgc agacaagga gcggagtta acgtacgac 
gatggagac actgagctt aaaagagag ttgtgtgtg 
gtcgaagga gtgaaaaag cttctaata gagcgatgc 
caagccgct tgggaaaaa ctgaacaag ctggaggaa 
gttcgattc aagttaaat ttggaatta aatgacaag 
acagaggct attgaaatt gataaggac caactgact 
ttggacaaa aattgtgcc aacataagc tttaaaacg 
gatccgcta cgaattgtt aaaaattcc ataccgtat 
gaagcctgg ctggaacat tcccgcttc atcaaagaa 
ctagcagac aacgaactt gcagacact cacaagctc 
cgagaggct ctgtttgtg gtgagagaa cgagctcga 
aatgatttg agagctcaa agagaccga gtggacttc 
actcttcgc aaaagaatc tacgaaacg caaaaagct 
cgcaacgaa cttgaatgg caacaattc aaaatgcga 
gaggaaatg gataagctc ctcaaagag attaaaaca 
ctcgaagat gcgttattg gcaaaaacc gacgcttta 
aaattatgt gaaactcgt ctagaaaat agagcttac 
cgtcctggt tttgaactt gccagagat gagactgaa 
acaggtctg aaaaatgaa gttacgcaa ctgaggcaa 
actagaaat gatctcata aataaaatt aactgtgct 
aaagccacg tataatgct ttggagaac cagcaagtt 
attattgat actgatttg gagaacaag agccattcg 
ttaatgact gatattagg tgtcttgat atgaggatt 
cggttgagg actggggag tttgcaggt cctggaact 
gacactgat agaaatatt cagttgacg aggatggaa 
caagaaata cctccgacg taa
    mRNA sequence
    • >TC002132 RA
      attaaaaca aatttcggg ccaatcatt atttaagaa 
tgaatcgtc ttacattca gtgttgcaa ttattataa 
gtgtcgtta gtgttacta ccggttccc cagggaaaa 
aattaaata tgttccata aacattttg ccgccgttg 
ataagttca aatgcaagg acccacaac ctcgagcta 
ttgctattt ccgaatttc taaacaaga gcataatta 
gcgaagcaa atttggtga gtatttgtg acacaattt 
ttattaagt aaagagttt taacacgaa atatttaca 
aaaacaaaa ttaacaata ttttttcag atgatttct 
ccagatatt acagtactt catagatgt ggacaggcg 
ggatgttgt cacaaaaac aaaacgtgt caagcacat 
aagactgaa accgtcaca ttggttttc ctaattaac 
aaaaaaaga agtcataaa gaatatatc gacgttacg 
gcacaaaat cacacagag tgtgaatgc caacctctc 
gtgaataat cctaaataa gtttacaga aatgtttta 
tttattttg tatttattt tgttttgtt agtcatacg 
aagccaagc tgtgatatt acctatcat aattgtcag 
tagtgtagt ttaaaatta atcaaatcg gttcagttt 
cttgcaaaa cttaacatg tcagtggta acgtacgaa 
aaacccatt caacacatt ggattagcc gactggcac 
gccaaacag tggcaaaac caacaaacc aatgacacc 
cgaagaacg gacgccttt aatttacga cacgagtcg 
agacaatta cgaaatgag actaatatt cggaccgaa 
tgggacact taccacaac aatgtgcgt ctagctgat 
cgcattaca gagcttgat aggtggaga gatgttcta 
acttgttgt ttagaacga gtcgataaa gaaatcctc 
tctctaaaa gacgagaaa ttttccacc gaaagggaa 
cttgatgct cttggaata cctctcgga gttgttgcc 
gagtgcatt tcgatgaga gactgcaga caaggagcg 
gagttaacg tacgacgat ggagacact gagcttaaa 
agagagttg tgtgtggtc gaaggagtg aaaaagctt 
ctaatagag cgatgccaa gccgcttgg gaaaaactg 
aacaagctg gaggaagtt cgattcaag ttaaatttg 
gaattaaat gacaagaca gaggctatt gaaattgat 
aaggaccaa ctgactttg gacaaaaat tgtgccaac 
ataagcttt aaaacggat ccgctacga attgttaaa 
aattccata ccgtatgaa gcctggctg gaacattcc 
cgcttcatc aaagaacta gcagacaac gaacttgca 
gacactcac aagctccga gaggctctg tttgtggtg 
agagaacga gctcgaaat gatttgaga gctcaaaga 
gaccgagtg gacttcact cttcgcaaa agaatctac 
gaaacgcaa aaagctcgc aacgaactt gaatggcaa 
caattcaaa atgcgagag gaaatggat aagctcctc 
aaagagatt aaaacactc gaagatgcg ttattggca 
aaaaccgac gctttaaaa ttatgtgaa actcgtcta 
gaaaataga gcttaccgt cctggtttt gaacttgcc 
agagatgag actgaaaca ggtctgaaa aatgaagtt 
acgcaactg aggcaaact agaaatgat ctcataaat 
aaaattaac tgtgctaaa gccacgtat aatgctttg 
gagaaccag caagttatt attgatact gatttggag 
aacaagagc cattcgtta atgactgat attaggtgt 
cttgatatg aggattcgg ttgaggact ggggagttt 
gcaggtcct ggaactgac actgataga aatattcag 
ttgacgagg atggaacaa gaaatacct ccgacgtaa 
actttaaat aaacgacct caggattac taaattaca 
agtggttcc acgtagtct ttttagtaa cagttattt 
ggtgtactt gtaatgtgt tttggcaat aaacttttt 
tatcaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY