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Gene information
General information
TC002132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 222819..231531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002132
    iBeetle screen iB_00332
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002132 RA
      MSVVTYEKP IQHIGLADW HAKQWQNQQ TNDTRRTDA 
      FNLRHESRQ LRNETNIRT EWDTYHNNV RLADRITEL 
      DRWRDVLTC CLERVDKEI LSLKDEKFS TERELDALG 
      IPLGVVAEC ISMRDCRQG AELTYDDGD TELKRELCV 
      VEGVKKLLI ERCQAAWEK LNKLEEVRF KLNLELNDK 
      TEAIEIDKD QLTLDKNCA NISFKTDPL RIVKNSIPY 
      EAWLEHSRF IKELADNEL ADTHKLREA LFVVRERAR 
      NDLRAQRDR VDFTLRKRI YETQKARNE LEWQQFKMR 
      EEMDKLLKE IKTLEDALL AKTDALKLC ETRLENRAY 
      RPGFELARD ETETGLKNE VTQLRQTRN DLINKINCA 
      KATYNALEN QQVIIDTDL ENKSHSLMT DIRCLDMRI 
      RLRTGEFAG PGTDTDRNI QLTRMEQEI PPT
    CDS sequence
    • >TC002132 RA
      atgtcagtg gtaacgtac gaaaaaccc attcaacac 
      attggatta gccgactgg cacgccaaa cagtggcaa 
      aaccaacaa accaatgac acccgaaga acggacgcc 
      tttaattta cgacacgag tcgagacaa ttacgaaat 
      gagactaat attcggacc gaatgggac acttaccac 
      aacaatgtg cgtctagct gatcgcatt acagagctt 
      gataggtgg agagatgtt ctaacttgt tgtttagaa 
      cgagtcgat aaagaaatc ctctctcta aaagacgag 
      aaattttcc accgaaagg gaacttgat gctcttgga 
      atacctctc ggagttgtt gccgagtgc atttcgatg 
      agagactgc agacaagga gcggagtta acgtacgac 
      gatggagac actgagctt aaaagagag ttgtgtgtg 
      gtcgaagga gtgaaaaag cttctaata gagcgatgc 
      caagccgct tgggaaaaa ctgaacaag ctggaggaa 
      gttcgattc aagttaaat ttggaatta aatgacaag 
      acagaggct attgaaatt gataaggac caactgact 
      ttggacaaa aattgtgcc aacataagc tttaaaacg 
      gatccgcta cgaattgtt aaaaattcc ataccgtat 
      gaagcctgg ctggaacat tcccgcttc atcaaagaa 
      ctagcagac aacgaactt gcagacact cacaagctc 
      cgagaggct ctgtttgtg gtgagagaa cgagctcga 
      aatgatttg agagctcaa agagaccga gtggacttc 
      actcttcgc aaaagaatc tacgaaacg caaaaagct 
      cgcaacgaa cttgaatgg caacaattc aaaatgcga 
      gaggaaatg gataagctc ctcaaagag attaaaaca 
      ctcgaagat gcgttattg gcaaaaacc gacgcttta 
      aaattatgt gaaactcgt ctagaaaat agagcttac 
      cgtcctggt tttgaactt gccagagat gagactgaa 
      acaggtctg aaaaatgaa gttacgcaa ctgaggcaa 
      actagaaat gatctcata aataaaatt aactgtgct 
      aaagccacg tataatgct ttggagaac cagcaagtt 
      attattgat actgatttg gagaacaag agccattcg 
      ttaatgact gatattagg tgtcttgat atgaggatt 
      cggttgagg actggggag tttgcaggt cctggaact 
      gacactgat agaaatatt cagttgacg aggatggaa 
      caagaaata cctccgacg taa
    mRNA sequence
    • >TC002132 RA
      attaaaaca aatttcggg ccaatcatt atttaagaa 
      tgaatcgtc ttacattca gtgttgcaa ttattataa 
      gtgtcgtta gtgttacta ccggttccc cagggaaaa 
      aattaaata tgttccata aacattttg ccgccgttg 
      ataagttca aatgcaagg acccacaac ctcgagcta 
      ttgctattt ccgaatttc taaacaaga gcataatta 
      gcgaagcaa atttggtga gtatttgtg acacaattt 
      ttattaagt aaagagttt taacacgaa atatttaca 
      aaaacaaaa ttaacaata ttttttcag atgatttct 
      ccagatatt acagtactt catagatgt ggacaggcg 
      ggatgttgt cacaaaaac aaaacgtgt caagcacat 
      aagactgaa accgtcaca ttggttttc ctaattaac 
      aaaaaaaga agtcataaa gaatatatc gacgttacg 
      gcacaaaat cacacagag tgtgaatgc caacctctc 
      gtgaataat cctaaataa gtttacaga aatgtttta 
      tttattttg tatttattt tgttttgtt agtcatacg 
      aagccaagc tgtgatatt acctatcat aattgtcag 
      tagtgtagt ttaaaatta atcaaatcg gttcagttt 
      cttgcaaaa cttaacatg tcagtggta acgtacgaa 
      aaacccatt caacacatt ggattagcc gactggcac 
      gccaaacag tggcaaaac caacaaacc aatgacacc 
      cgaagaacg gacgccttt aatttacga cacgagtcg 
      agacaatta cgaaatgag actaatatt cggaccgaa 
      tgggacact taccacaac aatgtgcgt ctagctgat 
      cgcattaca gagcttgat aggtggaga gatgttcta 
      acttgttgt ttagaacga gtcgataaa gaaatcctc 
      tctctaaaa gacgagaaa ttttccacc gaaagggaa 
      cttgatgct cttggaata cctctcgga gttgttgcc 
      gagtgcatt tcgatgaga gactgcaga caaggagcg 
      gagttaacg tacgacgat ggagacact gagcttaaa 
      agagagttg tgtgtggtc gaaggagtg aaaaagctt 
      ctaatagag cgatgccaa gccgcttgg gaaaaactg 
      aacaagctg gaggaagtt cgattcaag ttaaatttg 
      gaattaaat gacaagaca gaggctatt gaaattgat 
      aaggaccaa ctgactttg gacaaaaat tgtgccaac 
      ataagcttt aaaacggat ccgctacga attgttaaa 
      aattccata ccgtatgaa gcctggctg gaacattcc 
      cgcttcatc aaagaacta gcagacaac gaacttgca 
      gacactcac aagctccga gaggctctg tttgtggtg 
      agagaacga gctcgaaat gatttgaga gctcaaaga 
      gaccgagtg gacttcact cttcgcaaa agaatctac 
      gaaacgcaa aaagctcgc aacgaactt gaatggcaa 
      caattcaaa atgcgagag gaaatggat aagctcctc 
      aaagagatt aaaacactc gaagatgcg ttattggca 
      aaaaccgac gctttaaaa ttatgtgaa actcgtcta 
      gaaaataga gcttaccgt cctggtttt gaacttgcc 
      agagatgag actgaaaca ggtctgaaa aatgaagtt 
      acgcaactg aggcaaact agaaatgat ctcataaat 
      aaaattaac tgtgctaaa gccacgtat aatgctttg 
      gagaaccag caagttatt attgatact gatttggag 
      aacaagagc cattcgtta atgactgat attaggtgt 
      cttgatatg aggattcgg ttgaggact ggggagttt 
      gcaggtcct ggaactgac actgataga aatattcag 
      ttgacgagg atggaacaa gaaatacct ccgacgtaa 
      actttaaat aaacgacct caggattac taaattaca 
      agtggttcc acgtagtct ttttagtaa cagttattt 
      ggtgtactt gtaatgtgt tttggcaat aaacttttt 
      tatcaacg