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Gene information
General information
TC002075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6142138..6146675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002075
    iBeetle screen iB_00323

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002075 RA
      MMGIFKRRP RTGDSPSSI GAVISEPTN VNHDIHVSV 
      NAQGELEGL PSAWLRQIG TQITRDEQV NNPLAVKQA 
      VKFYNYSIK KQEKGEMKH IITEPLIDE ETKEIDKYM 
      NSKDAHKSK DGNLDDEGN EEPIYVNTI NRPETVPAI 
      PPKRTPTTN LKKAPKIPE QLDCLTIDE GTCELRRKS 
      SSDDDILRR KSGQTDEDY IRELKKLCN PGDPNDFYC 
      RSNRDLGTG AMGIVYAAT DLQTGQPVA IKDIDMTKQ 
      QRKELLLSE IAIMKNFQH KNLVNFLDA FVMYDGHLW 
      VVMELLDGG PLTDVVTET VMKEHHIAA VCHEVLQAI 
      DYLHSRGTI HRDIKSDNV LLSMDGSVK VTDFGFCAS 
      VVGNEQRET MVGTPYWMA PEVVTRQKY GKKVDIWSL 
      GIMIVEMLD GEPPYLREP PLRALYLIT ANGRPKIIR 
      WGELSYNLR DFIDRCLQV DVDQRATAE ELLKHEFLN 
      VRSELKSLT PLIKAAKKM LHKA
    CDS sequence
    • >TC002075 RA
      atgatgggt attttcaag cggcgcccc cgaaccggg 
      gacagcccc tcgtccatc ggggccgtg atcagcgag 
      cccaccaac gtcaaccac gatattcat gtgagtgtg 
      aacgcgcaa ggggagctc gaggggctg ccgtcggcc 
      tggctgcgc cagatcggc acccaaatc accagagat 
      gagcaagtc aacaacccc ctggcggtc aagcaggcc 
      gtcaagttc tacaactac tcgatcaag aagcaggag 
      aagggcgag atgaagcac atcatcacg gagcccctc 
      attgacgag gagacgaag gaaatcgac aagtacatg 
      aactcgaag gacgcgcac aagagcaag gacgggaat 
      ttggacgac gagggcaac gaagagccc atttatgta 
      aatacaata aaccgtccc gaaaccgtg ccagcaatc 
      cctccgaaa cgaacccca acgacaaat ttgaaaaaa 
      gcgccgaaa atccccgaa caactcgac tgtctgacc 
      atcgacgag ggcacgtgc gaactgcgt cgcaaatcc 
      tcctccgac gacgacatc ctccgacga aaaagtgga 
      caaaccgac gaagattac attcgcgaa ttgaaaaaa 
      ctctgtaat cctggagat cccaacgat ttttactgc 
      cgcagtaac cgggacctg gggaccggt gccatgggc 
      attgtgtac gccgccacc gacctccag accggccaa 
      ccggtcgca atcaaagac atcgacatg acgaaacaa 
      cagcgcaaa gagctgctt ttgagcgaa attgccata 
      atgaaaaat ttccaacac aaaaatttg gtgaatttt 
      ttggacgcg ttcgtgatg tatgacggc catttgtgg 
      gtggtgatg gagctgctg gacgggggg cctctgacg 
      gacgtcgtc accgaaacg gtgatgaag gagcaccac 
      atcgccgca gtgtgccat gaagtctta caggccatt 
      gattatctc cactcccga ggcaccatc caccgtgat 
      atcaaatct gataacgtc ctcctgagc atggacggc 
      tccgtcaag gtcaccgat ttcggcttc tgcgccagc 
      gtagtaggc aatgagcag cgcgaaaca atggtcggg 
      acgccatat tggatggca ccggaggtg gtaactcgt 
      caaaaatac ggcaaaaaa gtcgatatt tggtcactg 
      ggtataatg atagtcgaa atgttagac ggggaaccc 
      ccgtattta cgtgaaccg cctctaaga gcattgtat 
      ttaataaca gccaatggc aggccgaaa attataaga 
      tggggtgaa ttgagctat aatttgcgc gattttatc 
      gataggtgt ttgcaagtt gatgtcgat cagagggca 
      acagccgag gaattgtta aagcatgaa tttttaaat 
      gtgaggagt gaattgaaa tcgttgacg cctttgatt 
      aaagcagcg aaaaagatg ttgcataag gcgtga
    mRNA sequence
    • >TC002075 RA
      tgtgcgtca cgttggcga aaccataac caaattaat 
      tgttaaccg ttgctaatt aaatcaatt tggacaaac 
      gtgggcctt ctggctccc cccacgccc agtaaaatt 
      cgtttttac gcaacttta cccacgaat tttaccaag 
      attcacctt tggttggca atgctgcgt tgccaaccg 
      caaattccc taagtgggg tgtgtgcaa tgagcaatc 
      gagtgtgcc gtgtccgtg tgggggctt cagcacgtc 
      taaactaac ctcacagga tgatgggta ttttcaagc 
      ggcgccccc gaaccgggg acagcccct cgtccatcg 
      gggccgtga tcagcgagc ccaccaacg tcaaccacg 
      atattcatg tgagtgtga acgcgcaag gggagctcg 
      aggggctgc cgtcggcct ggctgcgcc agatcggca 
      cccaaatca ccagagatg agcaagtca acaaccccc 
      tggcggtca agcaggccg tcaagttct acaactact 
      cgatcaaga agcaggaga agggcgaga tgaagcaca 
      tcatcacgg agcccctca ttgacgagg agacgaagg 
      aaatcgaca agtacatga actcgaagg acgcgcaca 
      agagcaagg acgggaatt tggacgacg agggcaacg 
      aagagccca tttatgtaa atacaataa accgtcccg 
      aaaccgtgc cagcaatcc ctccgaaac gaaccccaa 
      cgacaaatt tgaaaaaag cgccgaaaa tccccgaac 
      aactcgact gtctgacca tcgacgagg gcacgtgcg 
      aactgcgtc gcaaatcct cctccgacg acgacatcc 
      tccgacgaa aaagtggac aaaccgacg aagattaca 
      ttcgcgaat tgaaaaaac tctgtaatc ctggagatc 
      ccaacgatt tttactgcc gcagtaacc gggacctgg 
      ggaccggtg ccatgggca ttgtgtacg ccgccaccg 
      acctccaga ccggccaac cggtcgcaa tcaaagaca 
      tcgacatga cgaaacaac agcgcaaag agctgcttt 
      tgagcgaaa ttgccataa tgaaaaatt tccaacaca 
      aaaatttgg tgaattttt tggacgcgt tcgtgatgt 
      atgacggcc atttgtggg tggtgatgg agctgctgg 
      acggggggc ctctgacgg acgtcgtca ccgaaacgg 
      tgatgaagg agcaccaca tcgccgcag tgtgccatg 
      aagtcttac aggccattg attatctcc actcccgag 
      gcaccatcc accgtgata tcaaatctg ataacgtcc 
      tcctgagca tggacggct ccgtcaagg tcaccgatt 
      tcggcttct gcgccagcg tagtaggca atgagcagc 
      gcgaaacaa tggtcggga cgccatatt ggatggcac 
      cggaggtgg taactcgtc aaaaatacg gcaaaaaag 
      tcgatattt ggtcactgg gtataatga tagtcgaaa 
      tgttagacg gggaacccc cgtatttac gtgaaccgc 
      ctctaagag cattgtatt taataacag ccaatggca 
      ggccgaaaa ttataagat ggggtgaat tgagctata 
      atttgcgcg attttatcg ataggtgtt tgcaagttg 
      atgtcgatc agagggcaa cagccgagg aattgttaa 
      agcatgaat ttttaaatg tgaggagtg aattgaaat 
      cgttgacgc ctttgatta aagcagcga aaaagatgt 
      tgcataagg cgtgactca cggcgattg tatttaggg 
      ggcggggcg aggcttgaa ggtggggcc taatatgtg 
      tggactgtg acgcatcta acacattcc agtttttcc 
      aaaaaataa gaataaatc attaccacg tcaaaacaa 
      gtagttacg aaaatatat atttatttt taaaacgat 
      acacagtgt gaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY