Status
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Gene information
General information
TC002003 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 207513..240413
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002003
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00322

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002003 RA
      MRLDSGVDL SESFSGLHI KNPDWNDLS CAKSKVQEP 
      PVETPTTAK DDLWKQYFQ QDEDGDTFL HLAIAEGFV 
      EVVLALIRK APHPLFLDT PNDNAQTPI HLAAAKQNW 
      LIVRWLVVA GAKPCPRNI RGDSPLHIA ARNGDLRTC 
      RAITDPVQE QERISLGLT YPKQPYQEI NLDQWNYEG 
      QTCVHVAAI EGHIDVLRH LVWYGADIN AREGRQGYT 
      ALHYSIVRG DERLAHFLL SECTKLNAD AVTYGGNSA 
      LQLGFPVPA TIAEALRSR GASSPFSTA SEDEYSDSE 
      TESNAYENA IFVRNLVDA SA
    • >TC002003 RB
      MSRVELYKT IFQQQHHFK VPDEGAVSE EEKCALDSG 
      FISGELRSE EKITEELPQ IEQPQENYM RLDSGVDLS 
      ESFSGLHIK NPDWNDLSC AKSKVQEPP VETPTTAKD 
      DLWKQYFQQ DEDGDTFLH LAIAEGFVE VVLALIRKA 
      PHPLFLDTP NDNAQTPIH LAAAKQNWL IVRWLVVAG 
      AKPCPRNIR GDSPLHIAA RNGDLRTCR AITDPVQEQ 
      ERISLGLTY PKQPYQEIN LDQWNYEGQ TCVHVAAIE 
      GHIDVLRHL VWYGADINA REGRQGYTA LHYSIVRGD 
      ERLAHFLLS ECTKLNADA VTYGGNSAL QLGFPVPAT 
      IAEALRSRG ASSPFSTAS EDEYSDSET ESNAYENAI 
      FVRNLVDAS A
    • >TC002003 RC
      MSGKQDKKI PSVPDEGAV SEEEKCALD SGFISGELR 
      SEEKITEEL PQIEQPQEN YMRLDSGVD LSESFSGLH 
      IKNPDWNDL SCAKSKVQE PPVETPTTA KDDLWKQYF 
      QQDEDGDTF LHLAIAEGF VEVVLALIR KAPHPLFLD 
      TPNDNAQTP IHLAAAKQN WLIVRWLVV AGAKPCPRN 
      IRGDSPLHI AARNGDLRT CRAITDPVQ EQERISLGL 
      TYPKQPYQE INLDQWNYE GQTCVHVAA IEGHIDVLR 
      HLVWYGADI NAREGRQGY TALHYSIVR GDERLAHFL 
      LSECTKLNA DAVTYGGNS ALQLGFPVP ATIAEALRS 
      RGASSPFST ASEDEYSDS ETESNAYEN AIFVRNLVD 
      ASA
    CDS sequence
    • >TC002003 RA
      atgcgtctg gacagtggc gtcgacttg tcagagagt 
      ttttccgga ctccatatc aaaaatccg gactggaac 
      gatctctcc tgtgctaag agtaaagtg caggaacca 
      cctgttgaa acaccaacg actgccaaa gacgacctc 
      tggaaacag tatttccaa caggatgag gatggcgat 
      acgttcctc cacttggcg attgccgaa ggattcgtc 
      gaggtagtc cttgcttta atcaggaaa gctccccac 
      cctttgttc cttgacacc cccaatgat aacgcccaa 
      acaccaatc cacctcgcc gctgccaaa cagaactgg 
      ctaatagtt cggtggttg gtggtggca ggtgctaaa 
      ccctgtccc agaaacatc cgaggtgat tcaccctta 
      catatagca gcacgaaat ggtgattta cgaacatgc 
      agagccatc accgatcca gtacaagaa caagaaagg 
      atatcactt ggtttaact taccccaaa cagccctat 
      caggagatc aatctcgac caatggaac tacgaaggt 
      cagacgtgc gtgcatgtg gctgccatc gaaggccac 
      atcgacgtt ctgcgccac ctggtgtgg tacggcgcc 
      gacatcaac gcccgagaa ggtcgccag ggttacacc 
      gccctccac tactccata gtgcggggc gatgaacgt 
      ctggcgcac tttttactc tccgaatgc acgaaattg 
      aacgccgac gccgtcacc tacggtgga aacagcgcc 
      ctccagctc ggcttcccg gtgccggcg accatcgcc 
      gaagcgctc cggtcacgg ggagcgtct tcgcccttc 
      tccaccgcc tcagaggac gagtacagc gacagcgaa 
      accgaaagt aacgcttac gagaatgcg attttcgta 
      cgaaattta gtcgacgcc agcgcttag
    • >TC002003 RB
      atgtcgcga gtggagtta tacaaaaca atcttccaa 
      caacaacat catttcaaa gttccagat gaaggcgct 
      gtgagcgaa gaggaaaag tgcgccctt gacagcggc 
      ttcatttcc ggtgagctc cgctctgag gagaaaatc 
      acagaggag ttgccccaa attgaacaa ccacaagag 
      aactacatg cgtctggac agtggcgtc gacttgtca 
      gagagtttt tccggactc catatcaaa aatccggac 
      tggaacgat ctctcctgt gctaagagt aaagtgcag 
      gaaccacct gttgaaaca ccaacgact gccaaagac 
      gacctctgg aaacagtat ttccaacag gatgaggat 
      ggcgatacg ttcctccac ttggcgatt gccgaagga 
      ttcgtcgag gtagtcctt gctttaatc aggaaagct 
      ccccaccct ttgttcctt gacaccccc aatgataac 
      gcccaaaca ccaatccac ctcgccgct gccaaacag 
      aactggcta atagttcgg tggttggtg gtggcaggt 
      gctaaaccc tgtcccaga aacatccga ggtgattca 
      cccttacat atagcagca cgaaatggt gatttacga 
      acatgcaga gccatcacc gatccagta caagaacaa 
      gaaaggata tcacttggt ttaacttac cccaaacag 
      ccctatcag gagatcaat ctcgaccaa tggaactac 
      gaaggtcag acgtgcgtg catgtggct gccatcgaa 
      ggccacatc gacgttctg cgccacctg gtgtggtac 
      ggcgccgac atcaacgcc cgagaaggt cgccagggt 
      tacaccgcc ctccactac tccatagtg cggggcgat 
      gaacgtctg gcgcacttt ttactctcc gaatgcacg 
      aaattgaac gccgacgcc gtcacctac ggtggaaac 
      agcgccctc cagctcggc ttcccggtg ccggcgacc 
      atcgccgaa gcgctccgg tcacgggga gcgtcttcg 
      cccttctcc accgcctca gaggacgag tacagcgac 
      agcgaaacc gaaagtaac gcttacgag aatgcgatt 
      ttcgtacga aatttagtc gacgccagc gcttag
    • >TC002003 RC
      atgtccggg aaacaggat aagaaaatc ccctcagtt 
      ccagatgaa ggcgctgtg agcgaagag gaaaagtgc 
      gcccttgac agcggcttc atttccggt gagctccgc 
      tctgaggag aaaatcaca gaggagttg ccccaaatt 
      gaacaacca caagagaac tacatgcgt ctggacagt 
      ggcgtcgac ttgtcagag agtttttcc ggactccat 
      atcaaaaat ccggactgg aacgatctc tcctgtgct 
      aagagtaaa gtgcaggaa ccacctgtt gaaacacca 
      acgactgcc aaagacgac ctctggaaa cagtatttc 
      caacaggat gaggatggc gatacgttc ctccacttg 
      gcgattgcc gaaggattc gtcgaggta gtccttgct 
      ttaatcagg aaagctccc caccctttg ttccttgac 
      acccccaat gataacgcc caaacacca atccacctc 
      gccgctgcc aaacagaac tggctaata gttcggtgg 
      ttggtggtg gcaggtgct aaaccctgt cccagaaac 
      atccgaggt gattcaccc ttacatata gcagcacga 
      aatggtgat ttacgaaca tgcagagcc atcaccgat 
      ccagtacaa gaacaagaa aggatatca cttggttta 
      acttacccc aaacagccc tatcaggag atcaatctc 
      gaccaatgg aactacgaa ggtcagacg tgcgtgcat 
      gtggctgcc atcgaaggc cacatcgac gttctgcgc 
      cacctggtg tggtacggc gccgacatc aacgcccga 
      gaaggtcgc cagggttac accgccctc cactactcc 
      atagtgcgg ggcgatgaa cgtctggcg cacttttta 
      ctctccgaa tgcacgaaa ttgaacgcc gacgccgtc 
      acctacggt ggaaacagc gccctccag ctcggcttc 
      ccggtgccg gcgaccatc gccgaagcg ctccggtca 
      cggggagcg tcttcgccc ttctccacc gcctcagag 
      gacgagtac agcgacagc gaaaccgaa agtaacgct 
      tacgagaat gcgattttc gtacgaaat ttagtcgac 
      gccagcgct tag
    mRNA sequence
    • >TC002003 RA
      tacgaagtg aaatgttcc gggaaatta ccgagtttg 
      tgattattc gggagattt tcttgttgc agttacaca 
      tttgagccc aaatgtcgc gagtggagt tatacaaaa 
      caatcttcc aacaacaac atcatttca aagttccag 
      atgaaggcg ctgtgagcg aagaggaaa agtgcgccc 
      ttgacagcg gcttcattt ccggtgagc tccgctctg 
      aggagaaaa tcacagagg agttgcccc aaattgaac 
      aaccacaag agaactaca tgcgtctgg acagtggcg 
      tcgacttgt cagagagtt tttccggac tccatatca 
      aaaatccgg actggaacg atctctcct gtgctaaga 
      gtaaagtgc aggaaccac ctgttgaaa caccaacga 
      ctgccaaag acgacctct ggaaacagt atttccaac 
      aggatgagg atggcgata cgttcctcc acttggcga 
      ttgccgaag gattcgtcg aggtagtcc ttgctttaa 
      tcaggaaag ctccccacc ctttgttcc ttgacaccc 
      ccaatgata acgcccaaa caccaatcc acctcgccg 
      ctgccaaac agaactggc taatagttc ggtggttgg 
      tggtggcag gtgctaaac cctgtccca gaaacatcc 
      gaggtgatt cacccttac atatagcag cacgaaatg 
      gtgatttac gaacatgca gagccatca ccgatccag 
      tacaagaac aagaaagga tatcacttg gtttaactt 
      accccaaac agccctatc aggagatca atctcgacc 
      aatggaact acgaaggtc agacgtgcg tgcatgtgg 
      ctgccatcg aaggccaca tcgacgttc tgcgccacc 
      tggtgtggt acggcgccg acatcaacg cccgagaag 
      gtcgccagg gttacaccg ccctccact actccatag 
      tgcggggcg atgaacgtc tggcgcact ttttactct 
      ccgaatgca cgaaattga acgccgacg ccgtcacct 
      acggtggaa acagcgccc tccagctcg gcttcccgg 
      tgccggcga ccatcgccg aagcgctcc ggtcacggg 
      gagcgtctt cgcccttct ccaccgcct cagaggacg 
      agtacagcg acagcgaaa ccgaaagta acgcttacg 
      agaatgcga ttttcgtac gaaatttag tcgacgcca 
      gcgcttaga acacagcga ttgaaacga cgaggaaga 
      ggagaacta atcgagcct tcggagcgc gcgaagcga 
      aggaggaat cagttgcca aatattaga tactaaaac 
      tgtgatcga tttcggcgg tcaaacaac aagtaacga 
      aagaatcgc atcggttta tttttttaa tttatgatg 
      ttttcttgt atatagtgg tgcgatttc ttgcaatgg 
      tcgagacga agtattgtg ttcttgtgt aaattattt 
      tttttttca aatttaatc ctgttgtat ttatttttc 
      tttttttgt tgttggatt atttctgta cataggcca 
      ctctagcgt tatctcgat tatttttag tttaatttt 
      agtccctaa aaacgacaa ataaaatta atagaaaac 
      t
    • >TC002003 RB
      tacgaagtg aaatgttcc gggaaatta ccgagtttg 
      tgattattc gggagattt tcttgttgc agttacaca 
      tttgagccc aaatgtcgc gagtggagt tatacaaaa 
      caatcttcc aacaacaac atcatttca aagttccag 
      atgaaggcg ctgtgagcg aagaggaaa agtgcgccc 
      ttgacagcg gcttcattt ccggtgagc tccgctctg 
      aggagaaaa tcacagagg agttgcccc aaattgaac 
      aaccacaag agaactaca tgcgtctgg acagtggcg 
      tcgacttgt cagagagtt tttccggac tccatatca 
      aaaatccgg actggaacg atctctcct gtgctaaga 
      gtaaagtgc aggaaccac ctgttgaaa caccaacga 
      ctgccaaag acgacctct ggaaacagt atttccaac 
      aggatgagg atggcgata cgttcctcc acttggcga 
      ttgccgaag gattcgtcg aggtagtcc ttgctttaa 
      tcaggaaag ctccccacc ctttgttcc ttgacaccc 
      ccaatgata acgcccaaa caccaatcc acctcgccg 
      ctgccaaac agaactggc taatagttc ggtggttgg 
      tggtggcag gtgctaaac cctgtccca gaaacatcc 
      gaggtgatt cacccttac atatagcag cacgaaatg 
      gtgatttac gaacatgca gagccatca ccgatccag 
      tacaagaac aagaaagga tatcacttg gtttaactt 
      accccaaac agccctatc aggagatca atctcgacc 
      aatggaact acgaaggtc agacgtgcg tgcatgtgg 
      ctgccatcg aaggccaca tcgacgttc tgcgccacc 
      tggtgtggt acggcgccg acatcaacg cccgagaag 
      gtcgccagg gttacaccg ccctccact actccatag 
      tgcggggcg atgaacgtc tggcgcact ttttactct 
      ccgaatgca cgaaattga acgccgacg ccgtcacct 
      acggtggaa acagcgccc tccagctcg gcttcccgg 
      tgccggcga ccatcgccg aagcgctcc ggtcacggg 
      gagcgtctt cgcccttct ccaccgcct cagaggacg 
      agtacagcg acagcgaaa ccgaaagta acgcttacg 
      agaatgcga ttttcgtac gaaatttag tcgacgcca 
      gcgcttaga acacagcga ttgaaacga cgaggaaga 
      ggagaacta atcgagcct tcggagcgc gcgaagcga 
      aggaggaat cagttgcca aatattaga tactaaaac 
      tgtgatcga tttcggcgg tcaaacaac aagtaacga 
      aagaatcgc atcggttta tttttttaa tttatgatg 
      ttttcttgt atatagtgg tgcgatttc ttgcaatgg 
      tcgagacga agtattgtg ttcttgtgt aaattattt 
      tttttttca aatttaatc ctgttgtat ttatttttc 
      tttttttgt tgttggatt atttctgta cataggcca 
      ctctagcgt tatctcgat tatttttag tttaatttt 
      agtccctaa aaacgacaa ataaaatta atagaaaac 
      t
    • >TC002003 RC
      tacgaagtg aaatgttcc gggaaatta ccgagtttg 
      tgattattc gggagattt tcttgttgc agttacaca 
      tttgagccc aaatgtcgc gagtggagt tatacaaaa 
      caatcttcc aacaacaac atcatttca aagcgcatg 
      tctggggaa tcccgaact tgtgttggg tgtccacca 
      cacgcgatc gcgttcatt tcttcaacc agtcgccga 
      gaatccatt ttcgcgtgt tgacgtaac gaacccaac 
      gacccaaaa tcgaaaaaa ccatcgtcg aaccatttc 
      caaccaccc cttggtcca agtcccaag tgtctcacg 
      gctgtcttt gcaaaaagt cgcgtgttg ttttggggg 
      ccgccatta gaaagtagt gcgtgcgga cttgtagcc 
      attttgtgt agactgctg tacttttaa gtgtgttcg 
      cttattatc gggaaaatg tccgggaaa caggataag 
      aaaatcccc tcagttcca gatgaaggc gctgtgagc 
      gaagaggaa aagtgcgcc cttgacagc ggcttcatt 
      tccggtgag ctccgctct gaggagaaa atcacagag 
      gagttgccc caaattgaa caaccacaa gagaactac 
      atgcgtctg gacagtggc gtcgacttg tcagagagt 
      ttttccgga ctccatatc aaaaatccg gactggaac 
      gatctctcc tgtgctaag agtaaagtg caggaacca 
      cctgttgaa acaccaacg actgccaaa gacgacctc 
      tggaaacag tatttccaa caggatgag gatggcgat 
      acgttcctc cacttggcg attgccgaa ggattcgtc 
      gaggtagtc cttgcttta atcaggaaa gctccccac 
      cctttgttc cttgacacc cccaatgat aacgcccaa 
      acaccaatc cacctcgcc gctgccaaa cagaactgg 
      ctaatagtt cggtggttg gtggtggca ggtgctaaa 
      ccctgtccc agaaacatc cgaggtgat tcaccctta 
      catatagca gcacgaaat ggtgattta cgaacatgc 
      agagccatc accgatcca gtacaagaa caagaaagg 
      atatcactt ggtttaact taccccaaa cagccctat 
      caggagatc aatctcgac caatggaac tacgaaggt 
      cagacgtgc gtgcatgtg gctgccatc gaaggccac 
      atcgacgtt ctgcgccac ctggtgtgg tacggcgcc 
      gacatcaac gcccgagaa ggtcgccag ggttacacc 
      gccctccac tactccata gtgcggggc gatgaacgt 
      ctggcgcac tttttactc tccgaatgc acgaaattg 
      aacgccgac gccgtcacc tacggtgga aacagcgcc 
      ctccagctc ggcttcccg gtgccggcg accatcgcc 
      gaagcgctc cggtcacgg ggagcgtct tcgcccttc 
      tccaccgcc tcagaggac gagtacagc gacagcgaa 
      accgaaagt aacgcttac gagaatgcg attttcgta 
      cgaaattta gtcgacgcc agcgcttag aacacagcg 
      attgaaacg acgaggaag aggagaact aatcgagcc 
      ttcggagcg cgcgaagcg aaggaggaa tcagttgcc 
      aaatattag atactaaaa ctgtgatcg atttcggcg 
      gtcaaacaa caagtaacg aaagaatcg catcggttt 
      attttttta atttatgat gttttcttg tatatagtg 
      gtgcgattt cttgcaatg gtcgagacg aagtattgt 
      gttcttgtg taaattatt ttttttttc aaatttaat 
      cctgttgta tttattttt ctttttttg ttgttggat 
      tatttctgt acataggcc actctagcg ttatctcga 
      ttattttta gtttaattt tagtcccta aaaacgaca 
      aataaaatt aatagaaaa ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY