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Gene information
General information
TC030942
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002163,TC001999
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid861: 133046..148047
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030942
    iBeetle screen iB_00338
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber partially not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 6

    iBeetle screen iB_00321
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupation delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    remarks: animals lost, larva lethality late (L7) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030942 RA
      MEQVAALKD KGNAALAAN KFEEAIKHY TEAIKLDPS 
      NHVLYSNRS AAFAKANNH ESALEDANK TVELKPDWA 
      KGYSRKGAA LAYLGRLDE AIATYERGL QIEPANPQL 
      QEGLQEVKA QKTAKGFPN PFNRPDLME KLRGDPRTK 
      PLLDDPNYV ATLHMLKTN PNLLGQKLQ DPNILTTLS 
      VLIGIDPGE EPMETEPVY TPPKKPEPK KEPEPDLPE 
      NKKLAKAAK EQGNEFYKK KNFEKAIEF YNQAIEHDP 
      TDITFYNNL AAVFFEQKD YDRCIKECE RAIDIGREN 
      RADFKLIAK SFLRIGNAY KRLKDYQNA KIYYEKSMS 
      EHRTPEIKT LLSDVEKII KEEERKAYV NPELAEKEK 
      EIGNELFKK GDYATAVKH YTEAIKRNP EDAKLYSNR 
      AACYTKLAA FDLGLKDCD KCVELDPKF IKGWIRKAH 
      ILQGMQQPT KAMSAFQKA LEIDPNNAE AINGYRACS 
      IENASGSGD PEKIRQRAM ADPEVQSIL RDPAMRMIL 
      EQMQNDPRA LQDHLKNPD IAAKIQKLL ESGLIAIH
    CDS sequence
    • >TC030942 RA
      atggaacaa gtcgccgct ttgaaagac aaaggcaat 
      gccgcactg gccgccaac aagttcgaa gaggcaatc 
      aaacactac accgaagcc atcaaactc gacccctcg 
      aaccacgtc ctgtactcc aaccgttcc gccgccttc 
      gccaaagcc aacaaccac gaatcggct ctcgaagac 
      gccaacaaa acggtcgaa ctgaagccc gactgggcc 
      aagggctac tcacggaag ggggctgcc ttggcatac 
      ctggggcgc ctcgacgag gccatcgcc acgtacgag 
      cgcggcttg cagatcgag cccgccaac ccccagctc 
      caggagggg ctgcaggag gtcaaagcc cagaaaaca 
      gccaagggg ttccccaat cctttcaac cgacctgac 
      ctcatggag aagcttcgc ggggacccc cggaccaag 
      cccctgctc gatgacccc aactacgtc gcgacgctc 
      cacatgctc aaaacaaac cccaatctc ctcggccag 
      aaactacaa gaccccaat attttgacc acgttgagt 
      gtcttgatt ggaatcgat ccgggggaa gaaccgatg 
      gagaccgaa ccggtgtac acgccaccg aagaagccc 
      gaaccgaag aaagaaccc gagccggac ttacccgag 
      aacaagaaa ctggcgaaa gcggcgaag gaacaaggg 
      aatgaattt tacaaaaag aagaatttt gaaaaagcg 
      atcgagttt tataatcag gcgattgaa cacgatccc 
      accgatatc acgttttat aataatttg gcggcggtt 
      tttttcgaa cagaaggat tacgatcga tgtattaag 
      gagtgcgag agggctatt gatataggg agggagaat 
      agagccgat tttaaatta attgcaaag tcgtttctt 
      aggattggg aatgcgtat aagagactg aaggattat 
      caaaatgcg aaaatttat tacgaaaag tcaatgtcc 
      gaacatagg acgcctgaa attaaaacg ctgttgtcc 
      gatgttgag aagattatc aaagaggag gaacggaaa 
      gcttatgtt aatcccgaa ttagctgaa aaagagaag 
      gaaattggt aatgaatta tttaagaaa ggtgattac 
      gccactgcc gtcaaacac tacaccgaa gccatcaaa 
      cgcaaccca gaagacgcc aaactgtac agtaaccgt 
      gctgcctgt tatacaaaa ctagctgcc tttgatctt 
      ggtttaaaa gactgcgac aagtgcgtt gagctcgat 
      ccgaaattc atcaaaggg tggatccga aaagcacac 
      attttgcaa ggaatgcaa caaccgacc aaagccatg 
      tcagctttc caaaaagcg ctcgaaatc gatccgaat 
      aatgccgaa gctattaac ggatataga gcatgcagt 
      atagaaaat gcgagcggt tcaggtgat cctgaaaag 
      ataagacaa cgtgcgatg gctgatcct gaagttcag 
      tcgattttg agagatccc gcaatgagg atgatcttg 
      gaacagatg caaaatgat ccaagagct ttgcaagac 
      cacttaaag aatcctgat atagctgca aagatacaa 
      aagttgcta gagtcgggg cttattgct attcattaa
    mRNA sequence
    • >TC030942 RA
      gtgtttttg tgattatta agtgccggt caaaatgga 
      acaagtcgc cgctttgaa agacaaagg caatgccgc 
      actggccgc caacaagtt cgaagaggc aatcaaaca 
      ctacaccga agccatcaa actcgaccc ctcgaacca 
      cgtcctgta ctccaaccg ttccgccgc cttcgccaa 
      agccaacaa ccacgaatc ggctctcga agacgccaa 
      caaaacggt cgaactgaa gcccgactg ggccaaggg 
      ctactcacg gaagggggc tgccttggc atacctggg 
      gcgcctcga cgaggccat cgccacgta cgagcgcgg 
      cttgcagat cgagcccgc caaccccca gctccagga 
      ggggctgca ggaggtcaa agcccagaa aacagccaa 
      ggggttccc caatccttt caaccgacc tgacctcat 
      ggagaagct tcgcgggga cccccggac caagcccct 
      gctcgatga ccccaacta cgtcgcgac gctccacat 
      gctcaaaac aaaccccaa tctcctcgg ccagaaact 
      acaagaccc caatatttt gaccacgtt gagtgtctt 
      gattggaat cgatccggg ggaagaacc gatggagac 
      cgaaccggt gtacacgcc accgaagaa gcccgaacc 
      gaagaaaga acccgagcc ggacttacc cgagaacaa 
      gaaactggc gaaagcggc gaaggaaca agggaatga 
      attttacaa aaagaagaa ttttgaaaa agcgatcga 
      gttttataa tcaggcgat tgaacacga tcccaccga 
      tatcacgtt ttataataa tttggcggc ggttttttt 
      cgaacagaa ggattacga tcgatgtat taaggagtg 
      cgagagggc tattgatat agggaggga gaatagagc 
      cgattttaa attaattgc aaagtcgtt tcttaggat 
      tgggaatgc gtataagag actgaagga ttatcaaaa 
      tgcgaaaat ttattacga aaagtcaat gtccgaaca 
      taggacgcc tgaaattaa aacgctgtt gtccgatgt 
      tgagaagat tatcaaaga ggaggaacg gaaagctta 
      tgttaatcc cgaattagc tgaaaaaga gaaggaaat 
      tggtaatga attatttaa gaaaggtga ttacgccac 
      tgccgtcaa acactacac cgaagccat caaacgcaa 
      cccagaaga cgccaaact gtacagtaa ccgtgctgc 
      ctgttatac aaaactagc tgcctttga tcttggttt 
      aaaagactg cgacaagtg cgttgagct cgatccgaa 
      attcatcaa agggtggat ccgaaaagc acacatttt 
      gcaaggaat gcaacaacc gaccaaagc catgtcagc 
      tttccaaaa agcgctcga aatcgatcc gaataatgc 
      cgaagctat taacggata tagagcatg cagtataga 
      aaatgcgag cggttcagg tgatcctga aaagataag 
      acaacgtgc gatggctga tcctgaagt tcagtcgat 
      tttgagaga tcccgcaat gaggatgat cttggaaca 
      gatgcaaaa tgatccaag agctttgca agaccactt 
      aaagaatcc tgatatagc tgcaaagat acaaaagtt 
      gctagagtc ggggcttat tgctattca ttaattttt 
      ggtttagtg atgaggaac gtgcgatta tgtttgtgt 
      tgaatagcg atcgaagcg catttagag tggttgcag 
      tatccagcg tttgatctt tttacttag cttaataac 
      cgtttaagt aataaattt acgttttta aagcacatt 
      tctatttat ttacttacc aacaagtct gaccacagt 
      gccctctac ttattgatt ataacttat aagtattgt 
      attcatttc ttttgtaat cattaaatt acgcttcaa 
      ata