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Gene information
General information
TC001979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid161: 117646..119754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001979
    iBeetle screen iB_00320
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, adult lethality - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    Adult morphology
    hindwing orientation irregular - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection



    thorax blistered - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    elytra not closed - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001979 RA
      MLLKIIAML KLLVFAFLL TVTRVSSRN DPKNDPRNN 
      GDLALWIDE KQVKMFSGM SMEIYAIVN GNVLPYILD 
      PNFEKYLPV IPSEVSYVN FTWKAGMKK YYYNFDRLQ 
      SFDESILEA PVISIKTKG RVPKRPKEF SILLPCLGN 
      SSGVAPFGI GLLIESRKG KPLNGTPLR LKLRKECSQ 
      RNPDPECDK KCANQGWCN NEKICQCSE GYMGQYCQT 
      ALCYPQCMN GGNCTSPGI CSCPPGFQG RHCEGGICG 
      EKCLNGGKC VQKDTCECS KGYYGPHCE YSKCIIPCL 
      NGGKCRGIN KCRCPQGFR GDHCEIGKA KPHRSSCKL 
      ACKHGTCVD NTCVCDPGW YGRLCHHMT M
    CDS sequence
    • >TC001979 RA
      atgttgctg aagataatt gcaatgttg aaattgctg 
      gttttcgcc tttctactg actgtaact cgtgtctca 
      tcccgcaat gaccctaaa aacgacccc cgaaataac 
      ggggaccta gccctatgg attgacgag aaacaggtc 
      aaaatgttc agtggtatg tcgatggaa atctacgca 
      attgtgaat ggcaatgta ctgccatac atattagat 
      ccgaacttt gaaaaatac ttaccagtc ataccttct 
      gaagttagt tatgttaat tttacttgg aaagcaggg 
      atgaagaag tattattat aattttgat cgattacag 
      tcatttgat gagagtatt ttagaagct ccagtgata 
      tcaattaaa acaaaagga agagtgccg aaacggcct 
      aaagaattt agtatttta ttgccatgt ttagggaac 
      agttcaggg gttgccccc tttggaata gggcttttg 
      attgaaagt agaaaggga aaacctctc aatgggacg 
      cctttgcgt ctcaaacta cgtaaagaa tgttcccag 
      agaaatcct gaccctgag tgtgataaa aaatgtgcc 
      aaccaaggc tggtgcaat aacgaaaaa atttgtcaa 
      tgctcagaa gggtacatg ggacagtac tgtcaaact 
      gcactgtgc tatccgcag tgcatgaac ggcggaaac 
      tgcacttct cctggcatt tgcagctgc cccccggga 
      ttccaaggc agacactgc gaaggaggt atttgcggc 
      gagaagtgc ctgaacggc gggaagtgc gtccagaaa 
      gacacctgc gagtgctcc aaaggctac tacgggccc 
      cactgcgaa tattccaaa tgcatcatc ccttgcctc 
      aacggcggg aaatgccgc ggcatcaac aaatgccga 
      tgtccccaa ggattccgc ggcgaccat tgcgaaatt 
      ggcaaggca aaaccgcac agaagcagc tgcaaactg 
      gcctgcaaa cacggcacc tgtgtggac aacacgtgc 
      gtttgcgac ccgggatgg tacggacga ctttgccat 
      cacatgaca atgtaa
    mRNA sequence
    • >TC001979 RA
      ctggttttg aaagccatt tttagtaga ggtcgctgc 
      caatgccgg aacttggtg catgcatat attatagaa 
      acgtattgt atccatgga agggagtga catcagttg 
      tatttacat tgtattttc tgagaatta tcgtaaatc 
      aaacataac ttgaatttg aatggtcgc cttaagtaa 
      actactgca tttctgccg agctgcgct tctccatct 
      ttaatttaa tattagttt gaatagatc gctatagga 
      aaccaggcg caatttgct ttaatgtag ctttctgcc 
      atccattaa actttgtgc taaatttct ggcactctc 
      aagaggaat gaagcttac gttattaat ggtaatttt 
      aaatttgat tcagtttgg ttaataaaa gaaattctt 
      tttctcgca atctttcta ttaataatc aaggttgct 
      acagaagtt aaaaataca tgtcggtaa gatgttatt 
      tttgtttta gcatgttgc tgaagataa ttgcaatgt 
      tgaaattgc tggttttcg cctttctac tgactgtaa 
      ctcgtgtct catcccgca atgacccta aaaacgacc 
      cccgaaata acggggacc tagccctat ggattgacg 
      agaaacagg tcaaaatgt tcagtggta tgtcgatgg 
      aaatctacg caattgtga atggcaatg tactgccat 
      acatattag atccgaact ttgaaaaat acttaccag 
      tcatacctt ctgaagtta gttatgtta attttactt 
      ggaaagcag ggatgaaga agtattatt ataattttg 
      atcgattac agtcatttg atgagagta ttttagaag 
      ctccagtga tatcaatta aaacaaaag gaagagtgc 
      cgaaacggc ctaaagaat ttagtattt tattgccat 
      gtttaggga acagttcag gggttgccc cctttggaa 
      tagggcttt tgattgaaa gtagaaagg gaaaacctc 
      tcaatggga cgcctttgc gtctcaaac tacgtaaag 
      aatgttccc agagaaatc ctgaccctg agtgtgata 
      aaaaatgtg ccaaccaag gctggtgca ataacgaaa 
      aaatttgtc aatgctcag aagggtaca tgggacagt 
      actgtcaaa ctgcactgt gctatccgc agtgcatga 
      acggcggaa actgcactt ctcctggca tttgcagct 
      gccccccgg gattccaag gcagacact gcgaaggag 
      gtatttgcg gcgagaagt gcctgaacg gcgggaagt 
      gcgtccaga aagacacct gcgagtgct ccaaaggct 
      actacgggc cccactgcg aatattcca aatgcatca 
      tcccttgcc tcaacggcg ggaaatgcc gcggcatca 
      acaaatgcc gatgtcccc aaggattcc gcggcgacc 
      attgcgaaa ttggcaagg caaaaccgc acagaagca 
      gctgcaaac tggcctgca aacacggca cctgtgtgg 
      acaacacgt gcgtttgcg acccgggat ggtacggac 
      gactttgcc atcacatga caatgtaac tgactttgc 
      catattgtt gtaacgacg aacactgat tttttaatc 
      actttgtta tactaaaat tattttgta aagtttgta 
      ttttatata tagtatatt tttaatgta agcttttgt 
      ttgtaatta agtgttttg tatgacaat cgcttgtga 
      tatagaata tgtgtattt ttttaacta ttttaataa 
      aattggcaa aaccgc