Status
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Gene information
General information
TC001975 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 25965..45330
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001975
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00319

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001975 RA
      MGGEKTRPM WWQTLPTML VLATIGSAS DGAICPARC 
      HCNDDTLTA SCGSAALEV VPIQLNPEV RHIDLSDNK 
      ITHVSFTFR FYNFLVTLD LSSNKIKNL GSSNFDMQH 
      NLKQLNLSR NDIEKISKD SFKGLRAVT SLDLSHNKL 
      EELKSETFR ELHSLQVLK LSQNRLVYL EEGIFKSAK 
      HLQELLLDH NLFLELPTG ALTDTLNLR FVSLASNLI 
      KSIKENQMP PLPELRTLL LQKNLINEI HQSSLSGLL 
      ALDHLDLSD NNFTVIPTP SLTKLSNIT KLKLSGNFI 
      STVPPVAFK GLFHLRFLR LDRQEILER IDTRAFVDN 
      INLERVWLD DNIAVDRLP TRLFHGNPR VTHLSVRNN 
      RLTNIEVTH FPLDQLRVL KLAGNPLEC NCSLSWLWH 
      LIQEQKSKR LFDLGNDTS EEEEGVSPG ELVVDLEDI 
      RCAGPEPLS DVLLADATE SQVDCSVSW IAAVSATLT 
      ALFIVVIVG GLLYWGPIK RACAKEKEL AGVESRCRN 
      KSVGACSNG RSEPFENIR TDKCMIAPP LIHHDYRTL 
      PSWDPYSAD CAGNMNIYE HLDLNNKTR PHIVYV
    CDS sequence
    • >TC001975 RA
      atggggggt gagaagacc cggcccatg tggtggcag 
      acgctacct accatgctg gtgctggcg acgatcgga 
      tctgccagc gacggagcc atctgtcct gcgaggtgc 
      cactgcaac gacgacact ctcaccgct tcttgtggc 
      tcagcagct ctcgaagtc gtccccatc cagctcaac 
      cccgaagta cgacatatc gacctttcc gacaacaaa 
      atcacacac gtctccttc acttttcga ttctacaac 
      tttctagta acgcttgat ctgtcgtcc aacaagatc 
      aaaaatctc ggatcgtca aactttgat atgcaacat 
      aatctcaaa caactcaat ctcagcaga aatgacatc 
      gagaagata tccaaggat tcgttcaaa gggctcagg 
      gctgtcacc agtcttgac ctcagccac aataaatta 
      gaagaactc aaatcggag acttttagg gaattgcac 
      tcccttcaa gtactcaaa ctatcacaa aatcgactg 
      gtttactta gaagaaggg atctttaaa tctgctaaa 
      catttacaa gaactactt ctggaccat aatctcttt 
      ctggagtta ccaacgggg gcattgact gatactctt 
      aatctgaga tttgtatca cttgctagt aaccttatc 
      aaatctatc aaggaaaat caaatgccg ccccttccc 
      gaattacga actctactg ctacaaaag aatcttatc 
      aatgagata catcaatcg tctttatcg ggtcttttg 
      gctctagac catctagat ctaagtgat aataacttt 
      acagtaata ccaacgccg tcactgacg aaattatca 
      aacataaca aaattaaag ttgagcggt aatttcata 
      agtaccgtc ccaccggtc gcttttaaa ggactcttc 
      catttgcgt ttccttaga ttagatcgc caagaaatc 
      ctcgagagg atagacacg agagcattc gtcgacaac 
      ataaacctt gagagagtt tggcttgac gataacata 
      gcggtagat agattacca acaaggtta tttcatggc 
      aatccccgt gttactcac ttgtctgta cgtaacaac 
      cgattaaca aacatagaa gtgacccat ttcccttta 
      gaccagtta cgtgttttg aaattagcc ggaaaccct 
      ttggagtgt aactgttct ttgtcgtgg ctctggcat 
      ttgattcaa gagcaaaaa tcgaaacga ctgtttgat 
      ttgggtaac gacacgtcg gaggaagaa gaaggtgta 
      agtcccggg gaattggtt gtagacttg gaggatata 
      agatgtgcc ggtccggag cctttgagt gatgtcctc 
      ttggcagac gcgaccgag tcccaagtg gactgttct 
      gtcagctgg attgcagcc gtttcggca acactaacg 
      gccctattt atagtcgtt atagtcggc ggtttgttg 
      tattggggc ccgataaag agggcatgt gccaaggag 
      aaggaactt gcaggagtc gagagcagg tgtcggaat 
      aaaagcgtc ggagcttgt tcgaacgga aggagtgaa 
      cctttcgag aacataagg acagataag tgcatgatc 
      gcgccacct ctcatacat cacgactac cgcacgttg 
      ccctcctgg gacccctac agcgcggac tgtgccggc 
      aatatgaac atttatgaa catttagat ttaaacaac 
      aagacgagg cctcatatc gtctacgtg taa
    mRNA sequence
    • >TC001975 RA
      cctaaaata tattgcttg tttcaggtg aacagaaca 
      aagcctccg actgaaacg ccgctttca cctgaatcc 
      atatctgcg gcggatata agtggattg actataaca 
      tcgttttgc aatcagatg aacaaatag aaaagatcc 
      catgacgga gcgtcgagc ctggcatgg ggggtgaga 
      agacccggc ccatgtggt ggcagacgc tacctacca 
      tgctggtgc tggcgacga tcggatctg ccagcgacg 
      gagccatct gtcctgcga ggtgccact gcaacgacg 
      acactctca ccgcttctt gtggctcag cagctctcg 
      aagtcgtcc ccatccagc tcaaccccg aagtacgac 
      atatcgacc tttccgaca acaaaatca cacacgtct 
      ccttcactt ttcgattct acaactttc tagtaacgc 
      ttgatctgt cgtccaaca agatcaaaa atctcggat 
      cgtcaaact ttgatatgc aacataatc tcaaacaac 
      tcaatctca gcagaaatg acatcgaga agatatcca 
      aggattcgt tcaaagggc tcagggctg tcaccagtc 
      ttgacctca gccacaata aattagaag aactcaaat 
      cggagactt ttagggaat tgcactccc ttcaagtac 
      tcaaactat cacaaaatc gactggttt acttagaag 
      aagggatct ttaaatctg ctaaacatt tacaagaac 
      tacttctgg accataatc tctttctgg agttaccaa 
      cgggggcat tgactgata ctcttaatc tgagatttg 
      tatcacttg ctagtaacc ttatcaaat ctatcaagg 
      aaaatcaaa tgccgcccc ttcccgaat tacgaactc 
      tactgctac aaaagaatc ttatcaatg agatacatc 
      aatcgtctt tatcgggtc ttttggctc tagaccatc 
      tagatctaa gtgataata actttacag taataccaa 
      cgccgtcac tgacgaaat tatcaaaca taacaaaat 
      taaagttga gcggtaatt tcataagta ccgtcccac 
      cggtcgctt ttaaaggac tcttccatt tgcgtttcc 
      ttagattag atcgccaag aaatcctcg agaggatag 
      acacgagag cattcgtcg acaacataa accttgaga 
      gagtttggc ttgacgata acatagcgg tagatagat 
      taccaacaa ggttatttc atggcaatc cccgtgtta 
      ctcacttgt ctgtacgta acaaccgat taacaaaca 
      tagaagtga cccatttcc ctttagacc agttacgtg 
      ttttgaaat tagccggaa accctttgg agtgtaact 
      gttctttgt cgtggctct ggcatttga ttcaagagc 
      aaaaatcga aacgactgt ttgatttgg gtaacgaca 
      cgtcggagg aagaagaag gtgtaagtc ccggggaat 
      tggttgtag acttggagg atataagat gtgccggtc 
      cggagcctt tgagtgatg tcctcttgg cagacgcga 
      ccgagtccc aagtggact gttctgtca gctggattg 
      cagccgttt cggcaacac taacggccc tatttatag 
      tcgttatag tcggcggtt tgttgtatt ggggcccga 
      taaagaggg catgtgcca aggagaagg aacttgcag 
      gagtcgaga gcaggtgtc ggaataaaa gcgtcggag 
      cttgttcga acggaagga gtgaacctt tcgagaaca 
      taaggacag ataagtgca tgatcgcgc cacctctca 
      tacatcacg actaccgca cgttgccct cctgggacc 
      cctacagcg cggactgtg ccggcaata tgaacattt 
      atgaacatt tagatttaa acaacaaga cgaggcctc 
      atatcgtct acgtgtaac tccagtgtg atctgtttg 
      tgcggatcg ctaggttct tcctaaaat tcgggtata 
      gaaatttgg cgagtgcgc ctgcattta tattttata 
      gaacacgtg tatcatatg ttatatgcc aagagctcg 
      actcggtct ggatacaaa acgctctgc caggagctt 
      ttcatcgag ctcgattca cattcaact cagtacaga 
      ttcaaaaag actgattcg tttttaaag tgtccgttt 
      ttgctactc caaaatgtg tcaaatagc tttaaatta 
      atgatgatg tgtaaagtt gtgacaaac taaagcaat 
      aaagattat ccgaaccga ctgcaatat cttattgca 
      tttgactac tcttcattt ttcttctga tctttcatg 
      ctgtcaacc gcggtactg gaccatgta tagctgttc 
      taacacaat ttactttag gaaccttta cacacgcag 
      taaaatatt ttactacat atatagatt taacaaaat 
      caaaatatt gtcatgtgt acatttgtt cacaataat 
      acctgttct taattatga ctactacta tttacttcc 
      tcctccata cgttacata atgtttatt aaattataa 
      gaaataaac aattacgaa aacacctct ctcattatt 
      ttcctttct tccaccggc acacactct cacacagat 
      aacacttca tcccgactt cagttatcg atcagaccc 
      cacattaaa ttatttcca agtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY