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Gene information
General information
TC001964 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid161: 171819..176264
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001964
    iBeetle screen iB_00318
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001964 RA
      MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV 
      HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK 
      LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK 
      LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL 
      LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD 
      LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY 
      FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID 
      GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT 
      LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW 
      LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS 
      INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK 
      ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI 
      VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKRFV
    • >TC001964 RB
      MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV 
      HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK 
      LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK 
      LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL 
      LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD 
      LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY 
      FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID 
      GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT 
      LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW 
      LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS 
      INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK 
      ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI 
      VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKSHIK
    • >TC001964 RC
      MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV 
      HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK 
      LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK 
      LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL 
      LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD 
      LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY 
      FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID 
      GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT 
      LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW 
      LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS 
      INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK 
      ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI 
      VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKRNELN 
      ESQSSRRCS QRKVNASLI TLGKNKVPE AKSLPKKKE 
      NLSWLIKNG AHETNVAKC SSDNKVENT VKRCKRTLA 
      LEKDKASPR SVKVLRRSH GQTSKDTKK SDGKLHLPS 
      VKINKTI
    CDS sequence
    • >TC001964 RA
      atggaagac ttttccaac gttctggtc aacactggc 
      gaattcata ccaggacga ctttatttc gtcacactt 
      aacactaac gtggccccg aaatccacc tcaaccgtt 
      cactatttc gccgtggac acggagaag tgctacgac 
      agcttctac aatgacttt ggcccccta aacctagcg 
      attctgttt cactactgc gggaaattg gaccggaaa 
      ctgtcgaat ggcactctc cagaataag aaaatcgtt 
      cactacacg ggccttgat gagcaaaac cgcgtcaac 
      gccgcgttt ctcataggg gcctatgct gttattaag 
      ctggattac gacccagaa caggcctac aaggtgctc 
      acaaggggg gcccaacaa agctatatc gaattcaga 
      gacgcctcg attggagag ccctacacc ctctccctg 
      cttgactgc ctcaaggcc gtcaaaaag gccctcgac 
      tgcggcttc ttcgacttt gagaacttc gattttttc 
      gaatatgaa cactacgag agggtggaa aacggcgat 
      ttgaactgg atcgttccg gagaagttc atcgcgttc 
      tgtgggccc caccacaag tcgatcatt gacaaggga 
      taccccatc cattccccc gagacatac ttcgcgtat 
      ttccgcaga cacaacgtc acaaccgtc atacgccta 
      aacaaaaag gcttacgat tcgaatcgc ttcgtgcag 
      gcgggtttt gaccacaaa gacttgttt tttatagac 
      gggggaatc cccaatgac cgaattttg aataaattt 
      atttcgatt tgtgagaac gcaaaaggt gtgatagcc 
      gttcattgc aaagcggga cttggacgc actggaacg 
      ttaattgcg tgttatatt atgaagcat tacaaattc 
      acggcgcaa gaggccatc gcctggatc aggatttgt 
      cggcctggg tccataata gcccaccag caaacttgg 
      ttgctagaa aaacaggaa caattgtgg gctgcgggc 
      gacgaatac cgacaacaa atgaaaatg acgcggcct 
      ataaaacac gaggtgggg atttacggc ctacgctcg 
      ataaataac aacaaaacc aagtcgaat gataacgtg 
      acggggatc atgaaaaag gttgattcc atggaaatc 
      agcgatgat aaagacttt gagtctgaa gaaaataaa 
      ataacgcaa ggggaccga ctgaacgaa atcaaggcg 
      caaagggcc aaaggcaag ccgcccctt ctatcaaat 
      ttgaccgaa cgtaaagcg ttccgctca aaagtaatc 
      gtaaacact ccaaccaca tctcccgtc cgagtgggg 
      agaataatg acaaacaca agcaagagc acaacttcg 
      actactaga gttctaaca aaaaggttt gtttag
    • >TC001964 RB
      atggaagac ttttccaac gttctggtc aacactggc 
      gaattcata ccaggacga ctttatttc gtcacactt 
      aacactaac gtggccccg aaatccacc tcaaccgtt 
      cactatttc gccgtggac acggagaag tgctacgac 
      agcttctac aatgacttt ggcccccta aacctagcg 
      attctgttt cactactgc gggaaattg gaccggaaa 
      ctgtcgaat ggcactctc cagaataag aaaatcgtt 
      cactacacg ggccttgat gagcaaaac cgcgtcaac 
      gccgcgttt ctcataggg gcctatgct gttattaag 
      ctggattac gacccagaa caggcctac aaggtgctc 
      acaaggggg gcccaacaa agctatatc gaattcaga 
      gacgcctcg attggagag ccctacacc ctctccctg 
      cttgactgc ctcaaggcc gtcaaaaag gccctcgac 
      tgcggcttc ttcgacttt gagaacttc gattttttc 
      gaatatgaa cactacgag agggtggaa aacggcgat 
      ttgaactgg atcgttccg gagaagttc atcgcgttc 
      tgtgggccc caccacaag tcgatcatt gacaaggga 
      taccccatc cattccccc gagacatac ttcgcgtat 
      ttccgcaga cacaacgtc acaaccgtc atacgccta 
      aacaaaaag gcttacgat tcgaatcgc ttcgtgcag 
      gcgggtttt gaccacaaa gacttgttt tttatagac 
      gggggaatc cccaatgac cgaattttg aataaattt 
      atttcgatt tgtgagaac gcaaaaggt gtgatagcc 
      gttcattgc aaagcggga cttggacgc actggaacg 
      ttaattgcg tgttatatt atgaagcat tacaaattc 
      acggcgcaa gaggccatc gcctggatc aggatttgt 
      cggcctggg tccataata gcccaccag caaacttgg 
      ttgctagaa aaacaggaa caattgtgg gctgcgggc 
      gacgaatac cgacaacaa atgaaaatg acgcggcct 
      ataaaacac gaggtgggg atttacggc ctacgctcg 
      ataaataac aacaaaacc aagtcgaat gataacgtg 
      acggggatc atgaaaaag gttgattcc atggaaatc 
      agcgatgat aaagacttt gagtctgaa gaaaataaa 
      ataacgcaa ggggaccga ctgaacgaa atcaaggcg 
      caaagggcc aaaggcaag ccgcccctt ctatcaaat 
      ttgaccgaa cgtaaagcg ttccgctca aaagtaatc 
      gtaaacact ccaaccaca tctcccgtc cgagtgggg 
      agaataatg acaaacaca agcaagagc acaacttcg 
      actactaga gttctaaca aaaagccat attaagtaa
    • >TC001964 RC
      atggaagac ttttccaac gttctggtc aacactggc 
      gaattcata ccaggacga ctttatttc gtcacactt 
      aacactaac gtggccccg aaatccacc tcaaccgtt 
      cactatttc gccgtggac acggagaag tgctacgac 
      agcttctac aatgacttt ggcccccta aacctagcg 
      attctgttt cactactgc gggaaattg gaccggaaa 
      ctgtcgaat ggcactctc cagaataag aaaatcgtt 
      cactacacg ggccttgat gagcaaaac cgcgtcaac 
      gccgcgttt ctcataggg gcctatgct gttattaag 
      ctggattac gacccagaa caggcctac aaggtgctc 
      acaaggggg gcccaacaa agctatatc gaattcaga 
      gacgcctcg attggagag ccctacacc ctctccctg 
      cttgactgc ctcaaggcc gtcaaaaag gccctcgac 
      tgcggcttc ttcgacttt gagaacttc gattttttc 
      gaatatgaa cactacgag agggtggaa aacggcgat 
      ttgaactgg atcgttccg gagaagttc atcgcgttc 
      tgtgggccc caccacaag tcgatcatt gacaaggga 
      taccccatc cattccccc gagacatac ttcgcgtat 
      ttccgcaga cacaacgtc acaaccgtc atacgccta 
      aacaaaaag gcttacgat tcgaatcgc ttcgtgcag 
      gcgggtttt gaccacaaa gacttgttt tttatagac 
      gggggaatc cccaatgac cgaattttg aataaattt 
      atttcgatt tgtgagaac gcaaaaggt gtgatagcc 
      gttcattgc aaagcggga cttggacgc actggaacg 
      ttaattgcg tgttatatt atgaagcat tacaaattc 
      acggcgcaa gaggccatc gcctggatc aggatttgt 
      cggcctggg tccataata gcccaccag caaacttgg 
      ttgctagaa aaacaggaa caattgtgg gctgcgggc 
      gacgaatac cgacaacaa atgaaaatg acgcggcct 
      ataaaacac gaggtgggg atttacggc ctacgctcg 
      ataaataac aacaaaacc aagtcgaat gataacgtg 
      acggggatc atgaaaaag gttgattcc atggaaatc 
      agcgatgat aaagacttt gagtctgaa gaaaataaa 
      ataacgcaa ggggaccga ctgaacgaa atcaaggcg 
      caaagggcc aaaggcaag ccgcccctt ctatcaaat 
      ttgaccgaa cgtaaagcg ttccgctca aaagtaatc 
      gtaaacact ccaaccaca tctcccgtc cgagtgggg 
      agaataatg acaaacaca agcaagagc acaacttcg 
      actactaga gttctaaca aaaagaaac gagttgaac 
      gaaagtcag tcgtcgcgt cgttgcagc caacgaaaa 
      gtcaacgct tcgcttata accctcgga aaaaataaa 
      gtaccggag gcgaaatcg ttgccgaag aaaaaagaa 
      aatttaagt tggttgatt aaaaatggc gcacatgaa 
      actaacgtt gctaagtgc agttctgat aataaagtt 
      gaaaatacg gtaaaacgc tgtaaaaga acgcttgcc 
      ttggagaaa gataaagca agtccacgt tcggttaaa 
      gtgttgcgg aggtcgcat ggacagacg tcgaaagac 
      actaaaaag tcggatggg aaattacat ttgccatcg 
      gtcaaaatc aataaaacg atttaa
    mRNA sequence
    • >TC001964 RA
      ggactctac aaataaatg ttaaactgg ttacatacc 
      cgtaaatta aagtttagt gattatcat tttccccca 
      aatacattt tttcttaac aaacaaatt gtttatcga 
      aattgcgga ccagacagc ccatggggt tgaccaaat 
      aagttagtt ttggtcaaa attgggcaa ttttgtcgc 
      gaaaatgcg cgagaattt gtgaaaatc ataccggtt 
      ggtgcccct gatgtcgtt gatgtcgcc attttattg 
      ataagtttc ttggattag atttggtgg ttgttgtgt 
      tattaaaca aaaaatgta aacgttttt taagccagt 
      ttttgcttg aaaacaaag tgaatttgg cgacgtacc 
      aacgaaatt gatcacata tgaagagga agagggcaa 
      ctaagaaaa tggaagact tttccaacg ttctggtca 
      acactggcg aattcatac caggacgac tttatttcg 
      tcacactta acactaacg tggccccga aatccacct 
      caaccgttc actatttcg ccgtggaca cggagaagt 
      gctacgaca gcttctaca atgactttg gccccctaa 
      acctagcga ttctgtttc actactgcg ggaaattgg 
      accggaaac tgtcgaatg gcactctcc agaataaga 
      aaatcgttc actacacgg gccttgatg agcaaaacc 
      gcgtcaacg ccgcgtttc tcatagggg cctatgctg 
      ttattaagc tggattacg acccagaac aggcctaca 
      aggtgctca caagggggg cccaacaaa gctatatcg 
      aattcagag acgcctcga ttggagagc cctacaccc 
      tctccctgc ttgactgcc tcaaggccg tcaaaaagg 
      ccctcgact gcggcttct tcgactttg agaacttcg 
      attttttcg aatatgaac actacgaga gggtggaaa 
      acggcgatt tgaactgga tcgttccgg agaagttca 
      tcgcgttct gtgggcccc accacaagt cgatcattg 
      acaagggat accccatcc attcccccg agacatact 
      tcgcgtatt tccgcagac acaacgtca caaccgtca 
      tacgcctaa acaaaaagg cttacgatt cgaatcgct 
      tcgtgcagg cgggttttg accacaaag acttgtttt 
      ttatagacg ggggaatcc ccaatgacc gaattttga 
      ataaattta tttcgattt gtgagaacg caaaaggtg 
      tgatagccg ttcattgca aagcgggac ttggacgca 
      ctggaacgt taattgcgt gttatatta tgaagcatt 
      acaaattca cggcgcaag aggccatcg cctggatca 
      ggatttgtc ggcctgggt ccataatag cccaccagc 
      aaacttggt tgctagaaa aacaggaac aattgtggg 
      ctgcgggcg acgaatacc gacaacaaa tgaaaatga 
      cgcggccta taaaacacg aggtgggga tttacggcc 
      tacgctcga taaataaca acaaaacca agtcgaatg 
      ataacgtga cggggatca tgaaaaagg ttgattcca 
      tggaaatca gcgatgata aagactttg agtctgaag 
      aaaataaaa taacgcaag gggaccgac tgaacgaaa 
      tcaaggcgc aaagggcca aaggcaagc cgccccttc 
      tatcaaatt tgaccgaac gtaaagcgt tccgctcaa 
      aagtaatcg taaacactc caaccacat ctcccgtcc 
      gagtgggga gaataatga caaacacaa gcaagagca 
      caacttcga ctactagag ttctaacaa aaaggtttg 
      tttagggtc attctagct ttctccagt gttatcatc 
      actatcaca aactagcat gacttttct ccccaatgc 
      atgatctaa tcacaacgg gaaaatatt gatacacta 
      ataaccaaa atttcgatt ttaaaccat cttttccct 
      ccagccata ttaagtaaa aggggtgga aacgtgaaa 
      attggccga agctgaacc agatttcca aatttccga 
      acgagtaag ttggaccaa aaaccgaaa atcgagtgg 
      agtaagatt ttggcatgt cacctccca tactattaa 
      taatcaccc agtattgga aatttgact acaaatccc 
      gaaattgca aacgctaat tccgcacta aaaacttca 
      gtttttcat ttcaacact cgtttgaac cgtttttct 
      cgcgctaaa tctacagtc gaattttga taagtcgca 
      aaaaacatt taagtatcg tttgtcttt ttgataacc 
      tgaactgtt tggctagca actcctagt tgagttatt 
      gaagttcga ctgtaaaaa tttgtaagt tttggcgga 
      attactttt gggcggttg ttcaggctt gcggtgggt 
      gtagtgagt aaaatcggc tatcaataa gttcctgtg 
      tgtgttcag tggtgtgga ttgtgtaat ttgatctga 
      ttttggcgt agattagcg gtagtttta tgtagcgca 
      tgcttacag tgcacggag agcttttta aatatcgta 
      gatcgaata attgcaatc cgcgttgtg gttttgtta 
      acagaaacg agttgaacg aaagtcagt cgtcgcgtc 
      gttgcagcc aacgaaaag tcaacgctt cgcttataa 
      ccctcggaa aaaataaag taccggagg cgaaatcgt 
      tgccgaaga aaaaagaaa atttaagtt ggttgatta 
      aaaatggcg cacatgaaa ctaacgttg ctaagtgca 
      gttctgata ataaagttg aaaatacgg taaaacgct 
      gtaaaagaa cgcttgcct tggagaaag ataaagcaa 
      gtccacgtt cggttaaag tgttgcgga ggtcgcatg 
      gacagacgt cgaaagaca ctaaaaagt cggatggga 
      aattacatt tgccatcgg tcaaaatca ataaaacga 
      tttaacttt ttcgtttgt gtacataac attaaactg 
      ttgaaagtt caaagttta attgtaaac actttgttt 
      ctaaatatt cacacaaac actcagtgt tagtactta 
      aaatgctat aagtgtggc tagactagt aatgccagt 
      attattttt tgtacaagg ttttacgaa attctcagc 
      tttaatctc ttcaaacct ggactgagt gtttctttt 
      gtttacaaa aactgtaca tagatgttg aaatacttt 
      aaataaatg tctatttta agttaaatt ttggactgt 
      taataaata taaatattg tttataatt agcagtaca 
      cgtttttat ataactcac taaactatt cgtttcgtt 
      attaatacg tttctttat tatagaaac caacacgaa 
      tgtatgctt taattgcca tgttagtgt ctaggtttt 
      cactgttac aatttcaac ttttttttg taatgaaat 
      tgtaaattc atctttgta atcattgtt cagtatttt 
      ttgaatcag ttttgtcaa taaatgttt cattgccat
    • >TC001964 RB
      ggactctac aaataaatg ttaaactgg ttacatacc 
      cgtaaatta aagtttagt gattatcat tttccccca 
      aatacattt tttcttaac aaacaaatt gtttatcga 
      aattgcgga ccagacagc ccatggggt tgaccaaat 
      aagttagtt ttggtcaaa attgggcaa ttttgtcgc 
      gaaaatgcg cgagaattt gtgaaaatc ataccggtt 
      ggtgcccct gatgtcgtt gatgtcgcc attttattg 
      ataagtttc ttggattag atttggtgg ttgttgtgt 
      tattaaaca aaaaatgta aacgttttt taagccagt 
      ttttgcttg aaaacaaag tgaatttgg cgacgtacc 
      aacgaaatt gatcacata tgaagagga agagggcaa 
      ctaagaaaa tggaagact tttccaacg ttctggtca 
      acactggcg aattcatac caggacgac tttatttcg 
      tcacactta acactaacg tggccccga aatccacct 
      caaccgttc actatttcg ccgtggaca cggagaagt 
      gctacgaca gcttctaca atgactttg gccccctaa 
      acctagcga ttctgtttc actactgcg ggaaattgg 
      accggaaac tgtcgaatg gcactctcc agaataaga 
      aaatcgttc actacacgg gccttgatg agcaaaacc 
      gcgtcaacg ccgcgtttc tcatagggg cctatgctg 
      ttattaagc tggattacg acccagaac aggcctaca 
      aggtgctca caagggggg cccaacaaa gctatatcg 
      aattcagag acgcctcga ttggagagc cctacaccc 
      tctccctgc ttgactgcc tcaaggccg tcaaaaagg 
      ccctcgact gcggcttct tcgactttg agaacttcg 
      attttttcg aatatgaac actacgaga gggtggaaa 
      acggcgatt tgaactgga tcgttccgg agaagttca 
      tcgcgttct gtgggcccc accacaagt cgatcattg 
      acaagggat accccatcc attcccccg agacatact 
      tcgcgtatt tccgcagac acaacgtca caaccgtca 
      tacgcctaa acaaaaagg cttacgatt cgaatcgct 
      tcgtgcagg cgggttttg accacaaag acttgtttt 
      ttatagacg ggggaatcc ccaatgacc gaattttga 
      ataaattta tttcgattt gtgagaacg caaaaggtg 
      tgatagccg ttcattgca aagcgggac ttggacgca 
      ctggaacgt taattgcgt gttatatta tgaagcatt 
      acaaattca cggcgcaag aggccatcg cctggatca 
      ggatttgtc ggcctgggt ccataatag cccaccagc 
      aaacttggt tgctagaaa aacaggaac aattgtggg 
      ctgcgggcg acgaatacc gacaacaaa tgaaaatga 
      cgcggccta taaaacacg aggtgggga tttacggcc 
      tacgctcga taaataaca acaaaacca agtcgaatg 
      ataacgtga cggggatca tgaaaaagg ttgattcca 
      tggaaatca gcgatgata aagactttg agtctgaag 
      aaaataaaa taacgcaag gggaccgac tgaacgaaa 
      tcaaggcgc aaagggcca aaggcaagc cgccccttc 
      tatcaaatt tgaccgaac gtaaagcgt tccgctcaa 
      aagtaatcg taaacactc caaccacat ctcccgtcc 
      gagtgggga gaataatga caaacacaa gcaagagca 
      caacttcga ctactagag ttctaacaa aaagccata 
      ttaagtaaa aggggtgga aacgtgaaa attggccga 
      agctgaacc agatttcca aatttccga acgagtaag 
      ttggaccaa aaaccgaaa atcgagtgg agtaagatt 
      ttggcatgt cacctccca tactattaa taatcaccc 
      agtattgga aatttgact acaaatccc gaaattgca 
      aacgctaat tccgcacta aaaacttca gtttttcat 
      ttcaacact cgtttgaac cgtttttct cgcgctaaa 
      tctacagtc gaattttga taagtcgca aaaaacatt 
      taagtatcg tttgtcttt ttgataacc tgaactgtt 
      tggctagca actcctagt tgagttatt gaagttcga 
      ctgtaaaaa tttgtaagt tttggcgga attactttt 
      gggcggttg ttcaggctt gcggtgggt gtagtgagt 
      aaaatcggc tatcaataa gttcctgtg tgtgttcag 
      tggtgtgga ttgtgtaat ttgatctga ttttggcgt 
      agattagcg gtagtttta tgtagcgca tgcttacag 
      tgcacggag agcttttta aatatcgta gatcgaata 
      attgcaatc cgcgttgtg gttttgtta acagaaacg 
      agttgaacg aaagtcagt cgtcgcgtc gttgcagcc 
      aacgaaaag tcaacgctt cgcttataa ccctcggaa 
      aaaataaag taccggagg cgaaatcgt tgccgaaga 
      aaaaagaaa atttaagtt ggttgatta aaaatggcg 
      cacatgaaa ctaacgttg ctaagtgca gttctgata 
      ataaagttg aaaatacgg taaaacgct gtaaaagaa 
      cgcttgcct tggagaaag ataaagcaa gtccacgtt 
      cggttaaag tgttgcgga ggtcgcatg gacagacgt 
      cgaaagaca ctaaaaagt cggatggga aattacatt 
      tgccatcgg tcaaaatca ataaaacga tttaacttt 
      ttcgtttgt gtacataac attaaactg ttgaaagtt 
      caaagttta attgtaaac actttgttt ctaaatatt 
      cacacaaac actcagtgt tagtactta aaatgctat 
      aagtgtggc tagactagt aatgccagt attattttt 
      tgtacaagg ttttacgaa attctcagc tttaatctc 
      ttcaaacct ggactgagt gtttctttt gtttacaaa 
      aactgtaca tagatgttg aaatacttt aaataaatg 
      tctatttta agttaaatt ttggactgt taataaata 
      taaatattg tttataatt agcagtaca cgtttttat 
      ataactcac taaactatt cgtttcgtt attaatacg 
      tttctttat tatagaaac caacacgaa tgtatgctt 
      taattgcca tgttagtgt ctaggtttt cactgttac 
      aatttcaac ttttttttg taatgaaat tgtaaattc 
      atctttgta atcattgtt cagtatttt ttgaatcag 
      ttttgtcaa taaatgttt cattgccat
    • >TC001964 RC
      ggactctac aaataaatg ttaaactgg ttacatacc 
      cgtaaatta aagtttagt gattatcat tttccccca 
      aatacattt tttcttaac aaacaaatt gtttatcga 
      aattgcgga ccagacagc ccatggggt tgaccaaat 
      aagttagtt ttggtcaaa attgggcaa ttttgtcgc 
      gaaaatgcg cgagaattt gtgaaaatc ataccggtt 
      ggtgcccct gatgtcgtt gatgtcgcc attttattg 
      ataagtttc ttggattag atttggtgg ttgttgtgt 
      tattaaaca aaaaatgta aacgttttt taagccagt 
      ttttgcttg aaaacaaag tgaatttgg cgacgtacc 
      aacgaaatt gatcacata tgaagagga agagggcaa 
      ctaagaaaa tggaagact tttccaacg ttctggtca 
      acactggcg aattcatac caggacgac tttatttcg 
      tcacactta acactaacg tggccccga aatccacct 
      caaccgttc actatttcg ccgtggaca cggagaagt 
      gctacgaca gcttctaca atgactttg gccccctaa 
      acctagcga ttctgtttc actactgcg ggaaattgg 
      accggaaac tgtcgaatg gcactctcc agaataaga 
      aaatcgttc actacacgg gccttgatg agcaaaacc 
      gcgtcaacg ccgcgtttc tcatagggg cctatgctg 
      ttattaagc tggattacg acccagaac aggcctaca 
      aggtgctca caagggggg cccaacaaa gctatatcg 
      aattcagag acgcctcga ttggagagc cctacaccc 
      tctccctgc ttgactgcc tcaaggccg tcaaaaagg 
      ccctcgact gcggcttct tcgactttg agaacttcg 
      attttttcg aatatgaac actacgaga gggtggaaa 
      acggcgatt tgaactgga tcgttccgg agaagttca 
      tcgcgttct gtgggcccc accacaagt cgatcattg 
      acaagggat accccatcc attcccccg agacatact 
      tcgcgtatt tccgcagac acaacgtca caaccgtca 
      tacgcctaa acaaaaagg cttacgatt cgaatcgct 
      tcgtgcagg cgggttttg accacaaag acttgtttt 
      ttatagacg ggggaatcc ccaatgacc gaattttga 
      ataaattta tttcgattt gtgagaacg caaaaggtg 
      tgatagccg ttcattgca aagcgggac ttggacgca 
      ctggaacgt taattgcgt gttatatta tgaagcatt 
      acaaattca cggcgcaag aggccatcg cctggatca 
      ggatttgtc ggcctgggt ccataatag cccaccagc 
      aaacttggt tgctagaaa aacaggaac aattgtggg 
      ctgcgggcg acgaatacc gacaacaaa tgaaaatga 
      cgcggccta taaaacacg aggtgggga tttacggcc 
      tacgctcga taaataaca acaaaacca agtcgaatg 
      ataacgtga cggggatca tgaaaaagg ttgattcca 
      tggaaatca gcgatgata aagactttg agtctgaag 
      aaaataaaa taacgcaag gggaccgac tgaacgaaa 
      tcaaggcgc aaagggcca aaggcaagc cgccccttc 
      tatcaaatt tgaccgaac gtaaagcgt tccgctcaa 
      aagtaatcg taaacactc caaccacat ctcccgtcc 
      gagtgggga gaataatga caaacacaa gcaagagca 
      caacttcga ctactagag ttctaacaa aaagaaacg 
      agttgaacg aaagtcagt cgtcgcgtc gttgcagcc 
      aacgaaaag tcaacgctt cgcttataa ccctcggaa 
      aaaataaag taccggagg cgaaatcgt tgccgaaga 
      aaaaagaaa atttaagtt ggttgatta aaaatggcg 
      cacatgaaa ctaacgttg ctaagtgca gttctgata 
      ataaagttg aaaatacgg taaaacgct gtaaaagaa 
      cgcttgcct tggagaaag ataaagcaa gtccacgtt 
      cggttaaag tgttgcgga ggtcgcatg gacagacgt 
      cgaaagaca ctaaaaagt cggatggga aattacatt 
      tgccatcgg tcaaaatca ataaaacga tttaacttt 
      ttcgtttgt gtacataac attaaactg ttgaaagtt 
      caaagttta attgtaaac actttgttt ctaaatatt 
      cacacaaac actcagtgt tagtactta aaatgctat 
      aagtgtggc tagactagt aatgccagt attattttt 
      tgtacaagg ttttacgaa attctcagc tttaatctc 
      ttcaaacct ggactgagt gtttctttt gtttacaaa 
      aactgtaca tagatgttg aaatacttt aaataaatg 
      tctatttta agttaaatt ttggactgt taataaata 
      taaatattg tttataatt agcagtaca cgtttttat 
      ataactcac taaactatt cgtttcgtt attaatacg 
      tttctttat tatagaaac caacacgaa tgtatgctt 
      taattgcca tgttagtgt ctaggtttt cactgttac 
      aatttcaac ttttttttg taatgaaat tgtaaattc 
      atctttgta atcattgtt cagtatttt ttgaatcag 
      ttttgtcaa taaatgttt cattgccat