| General information | |||
| TC001964 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450289.1: 171818..176263 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001964 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00318 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Days post injection
- >TC001964 RA
MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKRFV
- >TC001964 RB
MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKSHIK
- >TC001964 RC
MEDFSNVLV NTGEFIPGR LYFVTLNTN VAPKSTSTV HYFAVDTEK CYDSFYNDF GPLNLAILF HYCGKLDRK LSNGTLQNK KIVHYTGLD EQNRVNAAF LIGAYAVIK LDYDPEQAY KVLTRGAQQ SYIEFRDAS IGEPYTLSL LDCLKAVKK ALDCGFFDF ENFDFFEYE HYERVENGD LNWIVPEKF IAFCGPHHK SIIDKGYPI HSPETYFAY FRRHNVTTV IRLNKKAYD SNRFVQAGF DHKDLFFID GGIPNDRIL NKFISICEN AKGVIAVHC KAGLGRTGT LIACYIMKH YKFTAQEAI AWIRICRPG SIIAHQQTW LLEKQEQLW AAGDEYRQQ MKMTRPIKH EVGIYGLRS INNNKTKSN DNVTGIMKK VDSMEISDD KDFESEENK ITQGDRLNE IKAQRAKGK PPLLSNLTE RKAFRSKVI VNTPTTSPV RVGRIMTNT SKSTTSTTR VLTKRNELN ESQSSRRCS QRKVNASLI TLGKNKVPE AKSLPKKKE NLSWLIKNG AHETNVAKC SSDNKVENT VKRCKRTLA LEKDKASPR SVKVLRRSH GQTSKDTKK SDGKLHLPS VKINKTI
- >TC001964 RA
atggaagac ttttccaac gttctggtc aacactggc gaattcata ccaggacga ctttatttc gtcacactt aacactaac gtggccccg aaatccacc tcaaccgtt cactatttc gccgtggac acggagaag tgctacgac agcttctac aatgacttt ggcccccta aacctagcg attctgttt cactactgc gggaaattg gaccggaaa ctgtcgaat ggcactctc cagaataag aaaatcgtt cactacacg ggccttgat gagcaaaac cgcgtcaac gccgcgttt ctcataggg gcctatgct gttattaag ctggattac gacccagaa caggcctac aaggtgctc acaaggggg gcccaacaa agctatatc gaattcaga gacgcctcg attggagag ccctacacc ctctccctg cttgactgc ctcaaggcc gtcaaaaag gccctcgac tgcggcttc ttcgacttt gagaacttc gattttttc gaatatgaa cactacgag agggtggaa aacggcgat ttgaactgg atcgttccg gagaagttc atcgcgttc tgtgggccc caccacaag tcgatcatt gacaaggga taccccatc cattccccc gagacatac ttcgcgtat ttccgcaga cacaacgtc acaaccgtc atacgccta aacaaaaag gcttacgat tcgaatcgc ttcgtgcag gcgggtttt gaccacaaa gacttgttt tttatagac gggggaatc cccaatgac cgaattttg aataaattt atttcgatt tgtgagaac gcaaaaggt gtgatagcc gttcattgc aaagcggga cttggacgc actggaacg ttaattgcg tgttatatt atgaagcat tacaaattc acggcgcaa gaggccatc gcctggatc aggatttgt cggcctggg tccataata gcccaccag caaacttgg ttgctagaa aaacaggaa caattgtgg gctgcgggc gacgaatac cgacaacaa atgaaaatg acgcggcct ataaaacac gaggtgggg atttacggc ctacgctcg ataaataac aacaaaacc aagtcgaat gataacgtg acggggatc atgaaaaag gttgattcc atggaaatc agcgatgat aaagacttt gagtctgaa gaaaataaa ataacgcaa ggggaccga ctgaacgaa atcaaggcg caaagggcc aaaggcaag ccgcccctt ctatcaaat ttgaccgaa cgtaaagcg ttccgctca aaagtaatc gtaaacact ccaaccaca tctcccgtc cgagtgggg agaataatg acaaacaca agcaagagc acaacttcg actactaga gttctaaca aaaaggttt gtttag
- >TC001964 RB
atggaagac ttttccaac gttctggtc aacactggc gaattcata ccaggacga ctttatttc gtcacactt aacactaac gtggccccg aaatccacc tcaaccgtt cactatttc gccgtggac acggagaag tgctacgac agcttctac aatgacttt ggcccccta aacctagcg attctgttt cactactgc gggaaattg gaccggaaa ctgtcgaat ggcactctc cagaataag aaaatcgtt cactacacg ggccttgat gagcaaaac cgcgtcaac gccgcgttt ctcataggg gcctatgct gttattaag ctggattac gacccagaa caggcctac aaggtgctc acaaggggg gcccaacaa agctatatc gaattcaga gacgcctcg attggagag ccctacacc ctctccctg cttgactgc ctcaaggcc gtcaaaaag gccctcgac tgcggcttc ttcgacttt gagaacttc gattttttc gaatatgaa cactacgag agggtggaa aacggcgat ttgaactgg atcgttccg gagaagttc atcgcgttc tgtgggccc caccacaag tcgatcatt gacaaggga taccccatc cattccccc gagacatac ttcgcgtat ttccgcaga cacaacgtc acaaccgtc atacgccta aacaaaaag gcttacgat tcgaatcgc ttcgtgcag gcgggtttt gaccacaaa gacttgttt tttatagac gggggaatc cccaatgac cgaattttg aataaattt atttcgatt tgtgagaac gcaaaaggt gtgatagcc gttcattgc aaagcggga cttggacgc actggaacg ttaattgcg tgttatatt atgaagcat tacaaattc acggcgcaa gaggccatc gcctggatc aggatttgt cggcctggg tccataata gcccaccag caaacttgg ttgctagaa aaacaggaa caattgtgg gctgcgggc gacgaatac cgacaacaa atgaaaatg acgcggcct ataaaacac gaggtgggg atttacggc ctacgctcg ataaataac aacaaaacc aagtcgaat gataacgtg acggggatc atgaaaaag gttgattcc atggaaatc agcgatgat aaagacttt gagtctgaa gaaaataaa ataacgcaa ggggaccga ctgaacgaa atcaaggcg caaagggcc aaaggcaag ccgcccctt ctatcaaat ttgaccgaa cgtaaagcg ttccgctca aaagtaatc gtaaacact ccaaccaca tctcccgtc cgagtgggg agaataatg acaaacaca agcaagagc acaacttcg actactaga gttctaaca aaaagccat attaagtaa
- >TC001964 RC
atggaagac ttttccaac gttctggtc aacactggc gaattcata ccaggacga ctttatttc gtcacactt aacactaac gtggccccg aaatccacc tcaaccgtt cactatttc gccgtggac acggagaag tgctacgac agcttctac aatgacttt ggcccccta aacctagcg attctgttt cactactgc gggaaattg gaccggaaa ctgtcgaat ggcactctc cagaataag aaaatcgtt cactacacg ggccttgat gagcaaaac cgcgtcaac gccgcgttt ctcataggg gcctatgct gttattaag ctggattac gacccagaa caggcctac aaggtgctc acaaggggg gcccaacaa agctatatc gaattcaga gacgcctcg attggagag ccctacacc ctctccctg cttgactgc ctcaaggcc gtcaaaaag gccctcgac tgcggcttc ttcgacttt gagaacttc gattttttc gaatatgaa cactacgag agggtggaa aacggcgat ttgaactgg atcgttccg gagaagttc atcgcgttc tgtgggccc caccacaag tcgatcatt gacaaggga taccccatc cattccccc gagacatac ttcgcgtat ttccgcaga cacaacgtc acaaccgtc atacgccta aacaaaaag gcttacgat tcgaatcgc ttcgtgcag gcgggtttt gaccacaaa gacttgttt tttatagac gggggaatc cccaatgac cgaattttg aataaattt atttcgatt tgtgagaac gcaaaaggt gtgatagcc gttcattgc aaagcggga cttggacgc actggaacg ttaattgcg tgttatatt atgaagcat tacaaattc acggcgcaa gaggccatc gcctggatc aggatttgt cggcctggg tccataata gcccaccag caaacttgg ttgctagaa aaacaggaa caattgtgg gctgcgggc gacgaatac cgacaacaa atgaaaatg acgcggcct ataaaacac gaggtgggg atttacggc ctacgctcg ataaataac aacaaaacc aagtcgaat gataacgtg acggggatc atgaaaaag gttgattcc atggaaatc agcgatgat aaagacttt gagtctgaa gaaaataaa ataacgcaa ggggaccga ctgaacgaa atcaaggcg caaagggcc aaaggcaag ccgcccctt ctatcaaat ttgaccgaa cgtaaagcg ttccgctca aaagtaatc gtaaacact ccaaccaca tctcccgtc cgagtgggg agaataatg acaaacaca agcaagagc acaacttcg actactaga gttctaaca aaaagaaac gagttgaac gaaagtcag tcgtcgcgt cgttgcagc caacgaaaa gtcaacgct tcgcttata accctcgga aaaaataaa gtaccggag gcgaaatcg ttgccgaag aaaaaagaa aatttaagt tggttgatt aaaaatggc gcacatgaa actaacgtt gctaagtgc agttctgat aataaagtt gaaaatacg gtaaaacgc tgtaaaaga acgcttgcc ttggagaaa gataaagca agtccacgt tcggttaaa gtgttgcgg aggtcgcat ggacagacg tcgaaagac actaaaaag tcggatggg aaattacat ttgccatcg gtcaaaatc aataaaacg atttaa
- >TC001964 RA
ggactctac aaataaatg ttaaactgg ttacatacc cgtaaatta aagtttagt gattatcat tttccccca aatacattt tttcttaac aaacaaatt gtttatcga aattgcgga ccagacagc ccatggggt tgaccaaat aagttagtt ttggtcaaa attgggcaa ttttgtcgc gaaaatgcg cgagaattt gtgaaaatc ataccggtt ggtgcccct gatgtcgtt gatgtcgcc attttattg ataagtttc ttggattag atttggtgg ttgttgtgt tattaaaca aaaaatgta aacgttttt taagccagt ttttgcttg aaaacaaag tgaatttgg cgacgtacc aacgaaatt gatcacata tgaagagga agagggcaa ctaagaaaa tggaagact tttccaacg ttctggtca acactggcg aattcatac caggacgac tttatttcg tcacactta acactaacg tggccccga aatccacct caaccgttc actatttcg ccgtggaca cggagaagt gctacgaca gcttctaca atgactttg gccccctaa acctagcga ttctgtttc actactgcg ggaaattgg accggaaac tgtcgaatg gcactctcc agaataaga aaatcgttc actacacgg gccttgatg agcaaaacc gcgtcaacg ccgcgtttc tcatagggg cctatgctg ttattaagc tggattacg acccagaac aggcctaca aggtgctca caagggggg cccaacaaa gctatatcg aattcagag acgcctcga ttggagagc cctacaccc tctccctgc ttgactgcc tcaaggccg tcaaaaagg ccctcgact gcggcttct tcgactttg agaacttcg attttttcg aatatgaac actacgaga gggtggaaa acggcgatt tgaactgga tcgttccgg agaagttca tcgcgttct gtgggcccc accacaagt cgatcattg acaagggat accccatcc attcccccg agacatact tcgcgtatt tccgcagac acaacgtca caaccgtca tacgcctaa acaaaaagg cttacgatt cgaatcgct tcgtgcagg cgggttttg accacaaag acttgtttt ttatagacg ggggaatcc ccaatgacc gaattttga ataaattta tttcgattt gtgagaacg caaaaggtg tgatagccg ttcattgca aagcgggac ttggacgca ctggaacgt taattgcgt gttatatta tgaagcatt acaaattca cggcgcaag aggccatcg cctggatca ggatttgtc ggcctgggt ccataatag cccaccagc aaacttggt tgctagaaa aacaggaac aattgtggg ctgcgggcg acgaatacc gacaacaaa tgaaaatga cgcggccta taaaacacg aggtgggga tttacggcc tacgctcga taaataaca acaaaacca agtcgaatg ataacgtga cggggatca tgaaaaagg ttgattcca tggaaatca gcgatgata aagactttg agtctgaag aaaataaaa taacgcaag gggaccgac tgaacgaaa tcaaggcgc aaagggcca aaggcaagc cgccccttc tatcaaatt tgaccgaac gtaaagcgt tccgctcaa aagtaatcg taaacactc caaccacat ctcccgtcc gagtgggga gaataatga caaacacaa gcaagagca caacttcga ctactagag ttctaacaa aaaggtttg tttagggtc attctagct ttctccagt gttatcatc actatcaca aactagcat gacttttct ccccaatgc atgatctaa tcacaacgg gaaaatatt gatacacta ataaccaaa atttcgatt ttaaaccat cttttccct ccagccata ttaagtaaa aggggtgga aacgtgaaa attggccga agctgaacc agatttcca aatttccga acgagtaag ttggaccaa aaaccgaaa atcgagtgg agtaagatt ttggcatgt cacctccca tactattaa taatcaccc agtattgga aatttgact acaaatccc gaaattgca aacgctaat tccgcacta aaaacttca gtttttcat ttcaacact cgtttgaac cgtttttct cgcgctaaa tctacagtc gaattttga taagtcgca aaaaacatt taagtatcg tttgtcttt ttgataacc tgaactgtt tggctagca actcctagt tgagttatt gaagttcga ctgtaaaaa tttgtaagt tttggcgga attactttt gggcggttg ttcaggctt gcggtgggt gtagtgagt aaaatcggc tatcaataa gttcctgtg tgtgttcag tggtgtgga ttgtgtaat ttgatctga ttttggcgt agattagcg gtagtttta tgtagcgca tgcttacag tgcacggag agcttttta aatatcgta gatcgaata attgcaatc cgcgttgtg gttttgtta acagaaacg agttgaacg aaagtcagt cgtcgcgtc gttgcagcc aacgaaaag tcaacgctt cgcttataa ccctcggaa aaaataaag taccggagg cgaaatcgt tgccgaaga aaaaagaaa atttaagtt ggttgatta aaaatggcg cacatgaaa ctaacgttg ctaagtgca gttctgata ataaagttg aaaatacgg taaaacgct gtaaaagaa cgcttgcct tggagaaag ataaagcaa gtccacgtt cggttaaag tgttgcgga ggtcgcatg gacagacgt cgaaagaca ctaaaaagt cggatggga aattacatt tgccatcgg tcaaaatca ataaaacga tttaacttt ttcgtttgt gtacataac attaaactg ttgaaagtt caaagttta attgtaaac actttgttt ctaaatatt cacacaaac actcagtgt tagtactta aaatgctat aagtgtggc tagactagt aatgccagt attattttt tgtacaagg ttttacgaa attctcagc tttaatctc ttcaaacct ggactgagt gtttctttt gtttacaaa aactgtaca tagatgttg aaatacttt aaataaatg tctatttta agttaaatt ttggactgt taataaata taaatattg tttataatt agcagtaca cgtttttat ataactcac taaactatt cgtttcgtt attaatacg tttctttat tatagaaac caacacgaa tgtatgctt taattgcca tgttagtgt ctaggtttt cactgttac aatttcaac ttttttttg taatgaaat tgtaaattc atctttgta atcattgtt cagtatttt ttgaatcag ttttgtcaa taaatgttt cattgccat
- >TC001964 RB
ggactctac aaataaatg ttaaactgg ttacatacc cgtaaatta aagtttagt gattatcat tttccccca aatacattt tttcttaac aaacaaatt gtttatcga aattgcgga ccagacagc ccatggggt tgaccaaat aagttagtt ttggtcaaa attgggcaa ttttgtcgc gaaaatgcg cgagaattt gtgaaaatc ataccggtt ggtgcccct gatgtcgtt gatgtcgcc attttattg ataagtttc ttggattag atttggtgg ttgttgtgt tattaaaca aaaaatgta aacgttttt taagccagt ttttgcttg aaaacaaag tgaatttgg cgacgtacc aacgaaatt gatcacata tgaagagga agagggcaa ctaagaaaa tggaagact tttccaacg ttctggtca acactggcg aattcatac caggacgac tttatttcg tcacactta acactaacg tggccccga aatccacct caaccgttc actatttcg ccgtggaca cggagaagt gctacgaca gcttctaca atgactttg gccccctaa acctagcga ttctgtttc actactgcg ggaaattgg accggaaac tgtcgaatg gcactctcc agaataaga aaatcgttc actacacgg gccttgatg agcaaaacc gcgtcaacg ccgcgtttc tcatagggg cctatgctg ttattaagc tggattacg acccagaac aggcctaca aggtgctca caagggggg cccaacaaa gctatatcg aattcagag acgcctcga ttggagagc cctacaccc tctccctgc ttgactgcc tcaaggccg tcaaaaagg ccctcgact gcggcttct tcgactttg agaacttcg attttttcg aatatgaac actacgaga gggtggaaa acggcgatt tgaactgga tcgttccgg agaagttca tcgcgttct gtgggcccc accacaagt cgatcattg acaagggat accccatcc attcccccg agacatact tcgcgtatt tccgcagac acaacgtca caaccgtca tacgcctaa acaaaaagg cttacgatt cgaatcgct tcgtgcagg cgggttttg accacaaag acttgtttt ttatagacg ggggaatcc ccaatgacc gaattttga ataaattta tttcgattt gtgagaacg caaaaggtg tgatagccg ttcattgca aagcgggac ttggacgca ctggaacgt taattgcgt gttatatta tgaagcatt acaaattca cggcgcaag aggccatcg cctggatca ggatttgtc ggcctgggt ccataatag cccaccagc aaacttggt tgctagaaa aacaggaac aattgtggg ctgcgggcg acgaatacc gacaacaaa tgaaaatga cgcggccta taaaacacg aggtgggga tttacggcc tacgctcga taaataaca acaaaacca agtcgaatg ataacgtga cggggatca tgaaaaagg ttgattcca tggaaatca gcgatgata aagactttg agtctgaag aaaataaaa taacgcaag gggaccgac tgaacgaaa tcaaggcgc aaagggcca aaggcaagc cgccccttc tatcaaatt tgaccgaac gtaaagcgt tccgctcaa aagtaatcg taaacactc caaccacat ctcccgtcc gagtgggga gaataatga caaacacaa gcaagagca caacttcga ctactagag ttctaacaa aaagccata ttaagtaaa aggggtgga aacgtgaaa attggccga agctgaacc agatttcca aatttccga acgagtaag ttggaccaa aaaccgaaa atcgagtgg agtaagatt ttggcatgt cacctccca tactattaa taatcaccc agtattgga aatttgact acaaatccc gaaattgca aacgctaat tccgcacta aaaacttca gtttttcat ttcaacact cgtttgaac cgtttttct cgcgctaaa tctacagtc gaattttga taagtcgca aaaaacatt taagtatcg tttgtcttt ttgataacc tgaactgtt tggctagca actcctagt tgagttatt gaagttcga ctgtaaaaa tttgtaagt tttggcgga attactttt gggcggttg ttcaggctt gcggtgggt gtagtgagt aaaatcggc tatcaataa gttcctgtg tgtgttcag tggtgtgga ttgtgtaat ttgatctga ttttggcgt agattagcg gtagtttta tgtagcgca tgcttacag tgcacggag agcttttta aatatcgta gatcgaata attgcaatc cgcgttgtg gttttgtta acagaaacg agttgaacg aaagtcagt cgtcgcgtc gttgcagcc aacgaaaag tcaacgctt cgcttataa ccctcggaa aaaataaag taccggagg cgaaatcgt tgccgaaga aaaaagaaa atttaagtt ggttgatta aaaatggcg cacatgaaa ctaacgttg ctaagtgca gttctgata ataaagttg aaaatacgg taaaacgct gtaaaagaa cgcttgcct tggagaaag ataaagcaa gtccacgtt cggttaaag tgttgcgga ggtcgcatg gacagacgt cgaaagaca ctaaaaagt cggatggga aattacatt tgccatcgg tcaaaatca ataaaacga tttaacttt ttcgtttgt gtacataac attaaactg ttgaaagtt caaagttta attgtaaac actttgttt ctaaatatt cacacaaac actcagtgt tagtactta aaatgctat aagtgtggc tagactagt aatgccagt attattttt tgtacaagg ttttacgaa attctcagc tttaatctc ttcaaacct ggactgagt gtttctttt gtttacaaa aactgtaca tagatgttg aaatacttt aaataaatg tctatttta agttaaatt ttggactgt taataaata taaatattg tttataatt agcagtaca cgtttttat ataactcac taaactatt cgtttcgtt attaatacg tttctttat tatagaaac caacacgaa tgtatgctt taattgcca tgttagtgt ctaggtttt cactgttac aatttcaac ttttttttg taatgaaat tgtaaattc atctttgta atcattgtt cagtatttt ttgaatcag ttttgtcaa taaatgttt cattgccat
- >TC001964 RC
ggactctac aaataaatg ttaaactgg ttacatacc cgtaaatta aagtttagt gattatcat tttccccca aatacattt tttcttaac aaacaaatt gtttatcga aattgcgga ccagacagc ccatggggt tgaccaaat aagttagtt ttggtcaaa attgggcaa ttttgtcgc gaaaatgcg cgagaattt gtgaaaatc ataccggtt ggtgcccct gatgtcgtt gatgtcgcc attttattg ataagtttc ttggattag atttggtgg ttgttgtgt tattaaaca aaaaatgta aacgttttt taagccagt ttttgcttg aaaacaaag tgaatttgg cgacgtacc aacgaaatt gatcacata tgaagagga agagggcaa ctaagaaaa tggaagact tttccaacg ttctggtca acactggcg aattcatac caggacgac tttatttcg tcacactta acactaacg tggccccga aatccacct caaccgttc actatttcg ccgtggaca cggagaagt gctacgaca gcttctaca atgactttg gccccctaa acctagcga ttctgtttc actactgcg ggaaattgg accggaaac tgtcgaatg gcactctcc agaataaga aaatcgttc actacacgg gccttgatg agcaaaacc gcgtcaacg ccgcgtttc tcatagggg cctatgctg ttattaagc tggattacg acccagaac aggcctaca aggtgctca caagggggg cccaacaaa gctatatcg aattcagag acgcctcga ttggagagc cctacaccc tctccctgc ttgactgcc tcaaggccg tcaaaaagg ccctcgact gcggcttct tcgactttg agaacttcg attttttcg aatatgaac actacgaga gggtggaaa acggcgatt tgaactgga tcgttccgg agaagttca tcgcgttct gtgggcccc accacaagt cgatcattg acaagggat accccatcc attcccccg agacatact tcgcgtatt tccgcagac acaacgtca caaccgtca tacgcctaa acaaaaagg cttacgatt cgaatcgct tcgtgcagg cgggttttg accacaaag acttgtttt ttatagacg ggggaatcc ccaatgacc gaattttga ataaattta tttcgattt gtgagaacg caaaaggtg tgatagccg ttcattgca aagcgggac ttggacgca ctggaacgt taattgcgt gttatatta tgaagcatt acaaattca cggcgcaag aggccatcg cctggatca ggatttgtc ggcctgggt ccataatag cccaccagc aaacttggt tgctagaaa aacaggaac aattgtggg ctgcgggcg acgaatacc gacaacaaa tgaaaatga cgcggccta taaaacacg aggtgggga tttacggcc tacgctcga taaataaca acaaaacca agtcgaatg ataacgtga cggggatca tgaaaaagg ttgattcca tggaaatca gcgatgata aagactttg agtctgaag aaaataaaa taacgcaag gggaccgac tgaacgaaa tcaaggcgc aaagggcca aaggcaagc cgccccttc tatcaaatt tgaccgaac gtaaagcgt tccgctcaa aagtaatcg taaacactc caaccacat ctcccgtcc gagtgggga gaataatga caaacacaa gcaagagca caacttcga ctactagag ttctaacaa aaagaaacg agttgaacg aaagtcagt cgtcgcgtc gttgcagcc aacgaaaag tcaacgctt cgcttataa ccctcggaa aaaataaag taccggagg cgaaatcgt tgccgaaga aaaaagaaa atttaagtt ggttgatta aaaatggcg cacatgaaa ctaacgttg ctaagtgca gttctgata ataaagttg aaaatacgg taaaacgct gtaaaagaa cgcttgcct tggagaaag ataaagcaa gtccacgtt cggttaaag tgttgcgga ggtcgcatg gacagacgt cgaaagaca ctaaaaagt cggatggga aattacatt tgccatcgg tcaaaatca ataaaacga tttaacttt ttcgtttgt gtacataac attaaactg ttgaaagtt caaagttta attgtaaac actttgttt ctaaatatt cacacaaac actcagtgt tagtactta aaatgctat aagtgtggc tagactagt aatgccagt attattttt tgtacaagg ttttacgaa attctcagc tttaatctc ttcaaacct ggactgagt gtttctttt gtttacaaa aactgtaca tagatgttg aaatacttt aaataaatg tctatttta agttaaatt ttggactgt taataaata taaatattg tttataatt agcagtaca cgtttttat ataactcac taaactatt cgtttcgtt attaatacg tttctttat tatagaaac caacacgaa tgtatgctt taattgcca tgttagtgt ctaggtttt cactgttac aatttcaac ttttttttg taatgaaat tgtaaattc atctttgta atcattgtt cagtatttt ttgaatcag ttttgtcaa taaatgttt cattgccat
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