Status
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Gene information
General information
TC034858 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1610563..1624708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034858
    iBeetle screen iB_00316

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupation delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    iBeetle screen iB_03275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034858 RA
      MMVKFFVCL LLFFASDVR SMLNGKFKL DNSTDVLTI 
      KHVKNDVVL KSCVGFMIS FTRHWLVSS AFCLHSNPE 
      DKIIVEVGI EDGNNYKVI HYNDTKAVI HPGYDEKTG 
      KHDIGLLFV KETITDKFP YRLKVKFVH FPQMNGFVE 
      SWNMDPATG QKSTRVVYS LFDIYPMSE CKLKFAKFD 
      GIASLNEDF QVCAFPKEN GTGLCEPDS GAPLYVQLD 
      KQRQLYGIV GTFNDIFAV TSGRQHHCD PHHRHPVIF 
      TQLWPYFPW IAEITKYSP LIRLKLLIP VHHANISRC 
      IMFVYSGLV LWNFLAEEL STKVLAVFL MVLFLVRLV 
      QMLKEASAL PPGPWGLPI LGSLPFLKG DLHLHFRDL 
      THKYGSLIS TRLGSQLIV VLSDYKMIR DAFRKEEFT 
      GRPITEFTT LLDGYGVIN TAGKLWKDQ RRFLHDGLR 
      HFGMSYIGS RKTQMENRI MREVEEFLS VLTARKDTP 
      IDLNPVLAV SLSNVICDI LMSVRFSHN DERFKRFMF 
      LIDEGFKLF SSLEASFFI PILKYLPGQ RQTREKIAK 
      NRAEMAQFL QETIEEHRK SFDPSHLRD LLDTYLYEI 
      QKADEEGTG DHLFEGKDH DRQMQQIMG DLFSAGMET 
      IKSSLQWAV LFMLHHPEV MKAVQEELD QVVGRRRLP 
      KLEDLPYLP VTESTMLEV LRISSIVPM GTTHAPTRD 
      LKLNGFHLP RHAQIVPLL HSVHMDPSL WHEPERFNP 
      SRFINAEGK VVKPEYFLP FGVGRRMCL GEILARMEI 
      FSFFSSLLH SFDICVPTG ETLPSLKGV AGVTISPNA 
      FRVCLKPRP MEWDSMGTI RPAGSH
    CDS sequence
    • >TC034858 RA
      atgatggtc aagtttttt gtttgtttg ctgcttttc 
      tttgcctct gatgtcagg tcaatgttg aatggaaag 
      tttaaatta gacaatagc accgatgtt ttgaccatc 
      aaacatgta aaaaatgat gtagtgctg aaatcatgt 
      gtgggattt atgatttcc tttacgcga cattggctt 
      gtctcatca gctttttgt ctccatagc aatccggag 
      gataaaata atagtagaa gttggaatc gaagatgga 
      aacaattac aaagtcatt cattataat gacacaaaa 
      gctgtaatt catccaggt tatgacgag aagactgga 
      aaacacgac atcggtttg ctttttgtt aaggaaact 
      attaccgac aaatttcca tacagatta aaagttaaa 
      tttgttcat tttccccaa atgaatgga tttgtagaa 
      agctggaat atggaccca gcaactggc cagaaatca 
      actcgcgtt gtttattca ctctttgat atttatccc 
      atgtctgaa tgcaagttg aaatttgcg aaattcgac 
      ggtattgcg agtttaaat gaagatttt caagtgtgc 
      gcttttcca aaggaaaat ggaacaggt ttatgcgag 
      ccagactct ggtgcgcct ttatatgta caactggat 
      aaacagaga cagctctat ggaatagta gggacattc 
      aacgacatt tttgcagta acaagtgga cgacaacat 
      cattgtgac ccacatcat agacacccg gttattttt 
      acacaactt tggccctat tttccttgg attgccgaa 
      attactaaa tactcccca ctcattcgt cttaagttg 
      ctcatacct gtgcaccac gcgaatatt tcacggtgc 
      atcatgttc gtgtacagt ggcttagtg ttgtggaat 
      ttcctcgcc gaggagctg tccaccaaa gtgttggcc 
      gtcttcctc atggtactg ttcctggta cgtctcgtc 
      cagatgctg aaggaggcc agcgcgctg ccaccgggc 
      ccatggggg ttgcccatc ttgggctcg ctgcccttc 
      ctcaaaggc gacctgcac ctgcacttc agggatctg 
      acccataaa tacggctcg cttatctcg acccggctc 
      gggtcgcaa cttattgtc gtgttgagt gattacaag 
      atgattcgg gacgcgttc aggaaggag gagtttact 
      gggaggccc atcaccgaa tttaccaca cttttggac 
      ggttatggt gtgattaat acggccggg aaattgtgg 
      aaggaccag agacggttc ttacacgat ggtttgcga 
      cactttggg atgtcgtac atcgggagt cgcaaaaca 
      caaatggaa aatcgcatc atgagagag gtcgaagag 
      ttcctctcg gttctgacc gcgcgcaaa gatactccc 
      attgacttg aaccctgtc ttggccgtg tctttatcg 
      aacgtgatt tgtgatatt ttaatgtcg gtcagattc 
      tcgcataat gacgagcgt ttcaaacgc ttcatgttt 
      ctcattgac gaaggattc aaactgttc agttcactg 
      gaagcttcg ttcttcatc cccatcttg aaatattta 
      ccgggacaa cggcaaaca cgcgaaaaa atcgcaaag 
      aaccgtgcc gaaatggcg caattcctc caagaaacc 
      atcgaagag caccgcaaa agcttcgac ccatcccac 
      ctgcgtgac ctgttggac acctacttg tacgagatt 
      cagaaggcg gatgaggaa ggcaccggt gatcacctc 
      ttcgagggc aaagaccac gaccgtcag atgcagcaa 
      atcatgggc gatttgttc tccgccggg atggaaacc 
      atcaaaagt tcgctgcag tgggccgtc ctcttcatg 
      ctgcatcac cccgaagtg atgaaggca gtgcaagag 
      gagttggac caagtggtt ggacgtcgt cggctcccc 
      aagttggag gatttgccc tatttgcct gtgacggag 
      tcgacgatg ttggaagtg ttgcggatt tccagcatt 
      gttccaatg ggcacgaca cacgcaccc acaagggat 
      ttgaaactg aacgggttc catctgcca cggcatgcc 
      cagatcgtg ccccttctg cactctgtc cacatggac 
      cccagtctg tggcacgag ccggagcgc ttcaacccg 
      agccggttc atcaacgct gaaggcaaa gtggtcaag 
      cctgagtac tttctgccc ttcggggtg gggaggagg 
      atgtgtttg ggggagatc ttggcccgc atggagatc 
      ttctccttc ttctcctcg ctgctgcac tcgttcgac 
      atctgcgtg cccacgggg gagaccctc cccagcttg 
      aagggcgtg gcgggggtg accatcagt cccaacgcg 
      ttccgggtg tgtctcaag cccagaccc atggagtgg 
      gactcgatg ggcaccatc agaccagcg ggcagccac 
      tag
    mRNA sequence
    • >TC034858 RA
      tttaaaatc tcgcaatga tggtcaagt tttttgttt 
      gtttgctgc ttttctttg cctctgatg tcaggtcaa 
      tgttgaatg gaaagttta aattagaca atagcaccg 
      atgttttga ccatcaaac atgtaaaaa atgatgtag 
      tgctgaaat catgtgtgg gatttatga tttccttta 
      cgcgacatt ggcttgtct catcagctt tttgtctcc 
      atagcaatc cggaggata aaataatag tagaagttg 
      gaatcgaag atggaaaca attacaaag tcattcatt 
      ataatgaca caaaagctg taattcatc caggttatg 
      acgagaaga ctggaaaac acgacatcg gtttgcttt 
      ttgttaagg aaactatta ccgacaaat ttccataca 
      gattaaaag ttaaatttg ttcattttc cccaaatga 
      atggatttg tagaaagct ggaatatgg acccagcaa 
      ctggccaga aatcaactc gcgttgttt attcactct 
      ttgatattt atcccatgt ctgaatgca agttgaaat 
      ttgcgaaat tcgacggta ttgcgagtt taaatgaag 
      attttcaag tgtgcgctt ttccaaagg aaaatggaa 
      caggtttat gcgagccag actctggtg cgcctttat 
      atgtacaac tggataaac agagacagc tctatggaa 
      tagtaggga cattcaacg acatttttg cagtaacaa 
      gtggacgac aacatcatt gtgacccac atcatagac 
      acccggtta tttttacac aactttggc cctattttc 
      cttggattg ccgaaatta ctaaatact ccccactca 
      ttcgtctta agttgctca tacctgtgc accacgcga 
      atatttcac ggtgcatca tgttcgtgt acagtggct 
      tagtgttgt ggaatttcc tcgccgagg agctgtcca 
      ccaaagtgt tggccgtct tcctcatgg tactgttcc 
      tggtacgtc tcgtccaga tgctgaagg aggccagcg 
      cgctgccac cgggcccat gggggttgc ccatcttgg 
      gctcgctgc ccttcctca aaggcgacc tgcacctgc 
      acttcaggg atctgaccc ataaatacg gctcgctta 
      tctcgaccc ggctcgggt cgcaactta ttgtcgtgt 
      tgagtgatt acaagatga ttcgggacg cgttcagga 
      aggaggagt ttactggga ggcccatca ccgaattta 
      ccacacttt tggacggtt atggtgtga ttaatacgg 
      ccgggaaat tgtggaagg accagagac ggttcttac 
      acgatggtt tgcgacact ttgggatgt cgtacatcg 
      ggagtcgca aaacacaaa tggaaaatc gcatcatga 
      gagaggtcg aagagttcc tctcggttc tgaccgcgc 
      gcaaagata ctcccattg acttgaacc ctgtcttgg 
      ccgtgtctt tatcgaacg tgatttgtg atattttaa 
      tgtcggtca gattctcgc ataatgacg agcgtttca 
      aacgcttca tgtttctca ttgacgaag gattcaaac 
      tgttcagtt cactggaag cttcgttct tcatcccca 
      tcttgaaat atttaccgg gacaacggc aaacacgcg 
      aaaaaatcg caaagaacc gtgccgaaa tggcgcaat 
      tcctccaag aaaccatcg aagagcacc gcaaaagct 
      tcgacccat cccacctgc gtgacctgt tggacacct 
      acttgtacg agattcaga aggcggatg aggaaggca 
      ccggtgatc acctcttcg agggcaaag accacgacc 
      gtcagatgc agcaaatca tgggcgatt tgttctccg 
      ccgggatgg aaaccatca aaagttcgc tgcagtggg 
      ccgtcctct tcatgctgc atcaccccg aagtgatga 
      aggcagtgc aagaggagt tggaccaag tggttggac 
      gtcgtcggc tccccaagt tggaggatt tgccctatt 
      tgcctgtga cggagtcga cgatgttgg aagtgttgc 
      ggatttcca gcattgttc caatgggca cgacacacg 
      cacccacaa gggatttga aactgaacg ggttccatc 
      tgccacggc atgcccaga tcgtgcccc ttctgcact 
      ctgtccaca tggacccca gtctgtggc acgagccgg 
      agcgcttca acccgagcc ggttcatca acgctgaag 
      gcaaagtgg tcaagcctg agtactttc tgcccttcg 
      gggtgggga ggaggatgt gtttggggg agatcttgg 
      cccgcatgg agatcttct ccttcttct cctcgctgc 
      tgcactcgt tcgacatct gcgtgccca cgggggaga 
      ccctcccca gcttgaagg gcgtggcgg gggtgacca 
      tcagtccca acgcgttcc gggtgtgtc tcaagccca 
      gacccatgg agtgggact cgatgggca ccatcagac 
      cagcgggca gccactagt gggtcagga gcacggtga 
      ccgaatgcg tttcctata tttatatat cgaggcaca 
      gtacttaga cattcctca gttagataa gagatctcc 
      gacttgttc ggctgtgca ttgcaagtg ttcattcat 
      gttagtatt aatttttaa gtgctagtc acgtcatcg 
      tccagttgc cgaaaagta ccattgtat cacgttacc 
      tgctgtaaa ttggcaact ttcacacca ttttatcgt 
      tctaggtta agccacttg taaatatca ccctatttt 
      attttttga tcttgctaa ctgtacaag atgataatt 
      gtgtaatta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY