Status
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Gene information
General information
TC034265
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001935,TC001936
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1170807..1200459
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034265
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    iBeetle screen iB_08946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva potentially partiallyfragmented - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034265 RA
      LERLCYAEF AARVPKAGS AYVYSYVSV GEVVAYVIG 
      WNLILEYAI GTASVARGL SGYIDALAN QDIKNFMES 
      WAKMDVGFL ASYPDFLAF GFVVLLTAL LSIGVKESS 
      RLNNIFTGL NLITVGIVI VSGAIKADS SNWNKKLED 
      IPDKFREDA GTGGFMPFG VSGMMEGAA QCFYGFVGF 
      DAVATTGEE AKNPQRNIP LAIVISLLV IFLAYFGIS 
      TVLTMMWPY YDQDPTAPF PYVFDQLGW PAIKWIVTI 
      GAVIALCTS LLGALFPLP RIIYAMSND GLIFKFLAK 
      INPKTKTPI IATVLSGIL VGAMAILFD TDQLISMMS 
      IGTLLAYTI VAVCVLILR YQPTETQYP NLELKQNLE 
      DSYTLFGMF KQLFNLNMT KYASSSSAK ITNWSIILF 
      AVFTAAFDA FIIYAIDVL DKPYYMTLF IIVTLVMLT 
      LVVIIARQP VDEVKLSFK VPWVPFVPC LSIIINLYL 
      MLELDKDTW IRFGVWLFI VLSEVLMKM GLRTITFGQ 
      VLETLSRKK VVEFETGTK LAKVLTTLD LTALGVGST 
      LGVGIYVLA GDVAKNNAG PAVTVSFFI AAVTSILAG 
      LCYAEFGAR VPKAGSAYV YSYVCIGEL FAFIIGWNL 
      ILEYLIGSA TVVKALFLY LDELSNNVM SEFFQENIP 
      IEAGGELGQ YADIFSLGL SFVFAVAIA LGAKESTLV 
      NNIFTLVNL LVVATVVIS GLWKAKASN WSIPPEDVP 
      SGSGTGGFA PFGIKGVIQ GAARCFFAF IGFDCIATA 
      GEEAKTPSK SIPIGVVTS LLIVFFSYF GISTILTMM 
      LPYYEQHEK APLTHIYDV VGWPALKYV VSVGAICGL 
      FSSLLGAMF PLPRIIYAM ASDGLLFKA LAIVHPKFQ 
      TPFMGTLIA GSIAGCLAC IFDVDKLAN MMSIGTLLA 
      YSMVAACVL ILRYAVDEC EKKFEDKEE LTGTMYFKQ 
      MFNRKSQFP TRLTSSLVS WLVLAYFSV SFIFSGLIT 
      GFEKELENA EPWLLSLLC ILGVVLIVL LSIISWQPK 
      SSVQLTFAV PLVPWIPGI SIVVNVYLM TTLPAHTWE 
      YYGYWMLIG FLIYFAYGI WNSTERKSS KCSGTNNET 
      LPDDIKQNH L
    CDS sequence
    • >TC034265 RA
      ttagaaaga ttgtgttat gcagaattt gctgctcga 
      gtccctaag gcaggttct gcttacgtc tacagttac 
      gtttcagtg ggtgaagtt gtcgcatat gtaattgga 
      tggaattta atcttggaa tatgccata ggaaccgca 
      agtgtagct agaggtcta agcggttac atagacgct 
      cttgccaat caagatata aagaatttt atggaaagt 
      tgggcaaaa atggacgtt ggattttta gcatcgtat 
      cccgatttt ctagcgttt ggatttgtt gttcttctc 
      acagctcta ctttcaatc ggcgtgaaa gaatcttct 
      cgcctcaac aacatattc actggccta aatttgatc 
      actgtgggc atcgttata gtatctgga gccatcaaa 
      gccgactca tccaactgg aacaaaaaa ctcgaagac 
      attccagac aaatttaga gaagatgcc ggaactggt 
      ggttttatg ccttttgga gtctccgga atgatggaa 
      ggcgcagct caatgtttc tacggtttc gtgggtttc 
      gacgctgtt gccacaacc ggcgaagag gcgaaaaac 
      ccccagaga aacatccct ctagccatc gtcatttcc 
      cttcttgtg atctttctg gcctatttt ggcatttcg 
      acagttttg acaatgatg tggccctat tacgaccaa 
      gacccaact gctcctttt ccctatgtt ttcgaccaa 
      ttgggttgg cctgccatt aagtggatt gtaacaatt 
      ggggcagtt attgccctt tgtacgagt ctccttggt 
      gcgttgttt cctcttcct aggattatc tatgctatg 
      tcgaatgat gggttaatt ttcaaattt ttggccaaa 
      attaatcca aaaactaaa actccaatt attgcaact 
      gttctttcc ggaattcta gtaggtgca atggctata 
      cttttcgat actgaccaa ttgattagt atgatgtca 
      attgggact cttcttgcc tacactatc gttgcagtt 
      tgtgttcta atactgagg taccaaccc acagaaacg 
      caataccct aatctcgaa cttaagcaa aaccttgaa 
      gacagttat acgttgttt ggaatgttt aaacaactc 
      ttcaacttg aacatgacc aagtatgcc agttcaagc 
      tccgccaaa atcacaaat tggagtata atcctgttt 
      gctgttttc acagctgcc ttcgatgct ttcattatt 
      tacgccatt gatgttctc gataaacct tattacatg 
      acacttttt atcattgtt acgttagtt atgttgacc 
      cttgttgtg attattgca agacaaccg gtcgacgaa 
      gtcaaactc tcgtttaaa gttccttgg gtgcctttt 
      gttccttgt ttgagtatc atcattaat ttgtatctt 
      atgttggag ttggataag gatacttgg atacgattt 
      ggtgtctgg ttgtttatt gtgctaagt gaagtttta 
      atgaaaatg ggtctgcgc actatcaca tttggtcaa 
      gtgctggaa actctttcg aggaagaaa gttgtagaa 
      ttcgaaaca ggaacaaag ttggcaaaa gtgctcaca 
      actttggat ttgactgcc ttgggagtg ggtagtact 
      ttgggggtg ggtatatac gtactagct ggagatgtc 
      gccaaaaat aacgcggga cctgctgta acagtgtcg 
      ttttttatt gcagccgta acttcaatt cttgccggg 
      ctttgttat gctgaattt ggggcaagg gtgcccaaa 
      gctggctct gcctatgtt tacagttat gtgtgtata 
      ggagagtta ttcgcattt atcattgga tggaatttg 
      atactggaa tatctgata ggttctgca actgttgtc 
      aaagcgcta tttctttac ttagacgaa ctatcaaac 
      aacgtaatg tctgagttt tttcaagaa aatattccc 
      atcgaagcg ggaggagaa ctaggacaa tacgctgac 
      attttttct ttgggtcta tccttcgtt tttgctgtt 
      gccatagca ttaggagcg aaagaatct accttagtt 
      aataacata ttcactcta gtcaattta cttgtcgtt 
      gccactgtt gtaatctcc ggattatgg aaagccaaa 
      gcttccaac tggtctatt ccaccagag gatgtccct 
      tcagggagt ggcactggc ggttttgct ccttttggc 
      atcaaaggt gtgatccaa ggagccgcc agatgtttc 
      tttgctttc attggcttc gattgtatt gccacagca 
      ggtgaagag gcaaaaact ccaagcaaa tctattccc 
      ataggtgtg gtaacatca cttctcatc gtgtttttt 
      tcctacttt ggcatttcg acaatactt acaatgatg 
      ctaccatac tatgaacaa cacgagaaa gctcccttg 
      acccatatt tacgatgtg gttggttgg cctgccttg 
      aaatacgtg gtcagtgtt ggtgcaatt tgtggcctt 
      ttttcgagt ttattagga gctatgttt cctttaccc 
      aggattatt tacgccatg gcttccgat ggtctactt 
      ttcaaagct ttggctatt gttcatccc aagtttcag 
      acacctttt atggggact ttaattgct ggaagtatc 
      gcaggctgc ttggcttgt atttttgac gttgataag 
      ttggccaac atgatgtct attggaaca cttttggca 
      tattccatg gtagcagct tgtgttctt atcctgagg 
      tatgctgtt gacgaatgt gagaagaaa tttgaagac 
      aaggaagaa ttgactgga acaatgtat ttcaagcaa 
      atgtttaac agaaaaagt caatttccg acaaggtta 
      acttcatct ttagtttct tggctcgtt ttggcgtat 
      ttttcagtt tcttttatt ttctctgga cttatcact 
      ggtttcgaa aaagaattg gaaaatgcc gaaccttgg 
      cttctttcc cttctttgt atacttggt gtggtttta 
      attgttttg ttatccata atttcgtgg caacccaag 
      tcttcagta caactaaca tttgcagtt cctttagtt 
      ccttggatc cctggcatt agcatcgta gtcaatgtg 
      tatttaatg acgactttg ccagctcac acttgggaa 
      tattacggt tattggatg ctcataggt tttttgata 
      tatttcgct tatggcatt tggaacagt accgaacga 
      aaaagtagt aagtgttcc ggaacgaac aacgaaact 
      ttaccagac gatataaaa caaaaccat ttgtaa
    mRNA sequence
    • >TC034265 RA
      attagaaag attgtgtta tgcagaatt tgctgctcg 
      agtccctaa ggcaggttc tgcttacgt ctacagtta 
      cgtttcagt gggtgaagt tgtcgcata tgtaattgg 
      atggaattt aatcttgga atatgccat aggaaccgc 
      aagtgtagc tagaggtct aagcggtta catagacgc 
      tcttgccaa tcaagatat aaagaattt tatggaaag 
      ttgggcaaa aatggacgt tggattttt agcatcgta 
      tcccgattt tctagcgtt tggatttgt tgttcttct 
      cacagctct actttcaat cggcgtgaa agaatcttc 
      tcgcctcaa caacatatt cactggcct aaatttgat 
      cactgtggg catcgttat agtatctgg agccatcaa 
      agccgactc atccaactg gaacaaaaa actcgaaga 
      cattccaga caaatttag agaagatgc cggaactgg 
      tggttttat gccttttgg agtctccgg aatgatgga 
      aggcgcagc tcaatgttt ctacggttt cgtgggttt 
      cgacgctgt tgccacaac cggcgaaga ggcgaaaaa 
      cccccagag aaacatccc tctagccat cgtcatttc 
      ccttcttgt gatctttct ggcctattt tggcatttc 
      gacagtttt gacaatgat gtggcccta ttacgacca 
      agacccaac tgctccttt tccctatgt tttcgacca 
      attgggttg gcctgccat taagtggat tgtaacaat 
      tggggcagt tattgccct ttgtacgag tctccttgg 
      tgcgttgtt tcctcttcc taggattat ctatgctat 
      gtcgaatga tgggttaat tttcaaatt tttggccaa 
      aattaatcc aaaaactaa aactccaat tattgcaac 
      tgttctttc cggaattct agtaggtgc aatggctat 
      acttttcga tactgacca attgattag tatgatgtc 
      aattgggac tcttcttgc ctacactat cgttgcagt 
      ttgtgttct aatactgag gtaccaacc cacagaaac 
      gcaataccc taatctcga acttaagca aaaccttga 
      agacagtta tacgttgtt tggaatgtt taaacaact 
      cttcaactt gaacatgac caagtatgc cagttcaag 
      ctccgccaa aatcacaaa ttggagtat aatcctgtt 
      tgctgtttt cacagctgc cttcgatgc tttcattat 
      ttacgccat tgatgttct cgataaacc ttattacat 
      gacactttt tatcattgt tacgttagt tatgttgac 
      ccttgttgt gattattgc aagacaacc ggtcgacga 
      agtcaaact ctcgtttaa agttccttg ggtgccttt 
      tgttccttg tttgagtat catcattaa tttgtatct 
      tatgttgga gttggataa ggatacttg gatacgatt 
      tggtgtctg gttgtttat tgtgctaag tgaagtttt 
      aatgaaaat gggtctgcg cactatcac atttggtca 
      agtgctgga aactctttc gaggaagaa agttgtaga 
      attcgaaac aggaacaaa gttggcaaa agtgctcac 
      aactttgga tttgactgc cttgggagt gggtagtac 
      tttgggggt gggtatata cgtactagc tggagatgt 
      cgccaaaaa taacgcggg acctgctgt aacagtgtc 
      gttttttat tgcagccgt aacttcaat tcttgccgg 
      gctttgtta tgctgaatt tggggcaag ggtgcccaa 
      agctggctc tgcctatgt ttacagtta tgtgtgtat 
      aggagagtt attcgcatt tatcattgg atggaattt 
      gatactgga atatctgat aggttctgc aactgttgt 
      caaagcgct atttcttta cttagacga actatcaaa 
      caacgtaat gtctgagtt ttttcaaga aaatattcc 
      catcgaagc gggaggaga actaggaca atacgctga 
      cattttttc tttgggtct atccttcgt ttttgctgt 
      tgccatagc attaggagc gaaagaatc taccttagt 
      taataacat attcactct agtcaattt acttgtcgt 
      tgccactgt tgtaatctc cggattatg gaaagccaa 
      agcttccaa ctggtctat tccaccaga ggatgtccc 
      ttcagggag tggcactgg cggttttgc tccttttgg 
      catcaaagg tgtgatcca aggagccgc cagatgttt 
      ctttgcttt cattggctt cgattgtat tgccacagc 
      aggtgaaga ggcaaaaac tccaagcaa atctattcc 
      cataggtgt ggtaacatc acttctcat cgtgttttt 
      ttcctactt tggcatttc gacaatact tacaatgat 
      gctaccata ctatgaaca acacgagaa agctccctt 
      gacccatat ttacgatgt ggttggttg gcctgcctt 
      gaaatacgt ggtcagtgt tggtgcaat ttgtggcct 
      tttttcgag tttattagg agctatgtt tcctttacc 
      caggattat ttacgccat ggcttccga tggtctact 
      tttcaaagc tttggctat tgttcatcc caagtttca 
      gacaccttt tatggggac tttaattgc tggaagtat 
      cgcaggctg cttggcttg tatttttga cgttgataa 
      gttggccaa catgatgtc tattggaac acttttggc 
      atattccat ggtagcagc ttgtgttct tatcctgag 
      gtatgctgt tgacgaatg tgagaagaa atttgaaga 
      caaggaaga attgactgg aacaatgta tttcaagca 
      aatgtttaa cagaaaaag tcaatttcc gacaaggtt 
      aacttcatc tttagtttc ttggctcgt tttggcgta 
      tttttcagt ttcttttat tttctctgg acttatcac 
      tggtttcga aaaagaatt ggaaaatgc cgaaccttg 
      gcttctttc ccttctttg tatacttgg tgtggtttt 
      aattgtttt gttatccat aatttcgtg gcaacccaa 
      gtcttcagt acaactaac atttgcagt tcctttagt 
      tccttggat ccctggcat tagcatcgt agtcaatgt 
      gtatttaat gacgacttt gccagctca cacttggga 
      atattacgg ttattggat gctcatagg ttttttgat 
      atatttcgc ttatggcat ttggaacag taccgaacg 
      aaaaagtag taagtgttc cggaacgaa caacgaaac 
      tttaccaga cgatataaa acaaaacca tttgtaaac 
      ctcattcca acataattt atttattac caaaacaca 
      aaacaataa agacaacaa accaaa
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