Status
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Gene information
General information
TC034265
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001935,TC001936 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1170807..1200459
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034265
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    iBeetle screen iB_08946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva potentially partiallyfragmented - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034265 RA
      LERLCYAEF AARVPKAGS AYVYSYVSV GEVVAYVIG 
WNLILEYAI GTASVARGL SGYIDALAN QDIKNFMES 
WAKMDVGFL ASYPDFLAF GFVVLLTAL LSIGVKESS 
RLNNIFTGL NLITVGIVI VSGAIKADS SNWNKKLED 
IPDKFREDA GTGGFMPFG VSGMMEGAA QCFYGFVGF 
DAVATTGEE AKNPQRNIP LAIVISLLV IFLAYFGIS 
TVLTMMWPY YDQDPTAPF PYVFDQLGW PAIKWIVTI 
GAVIALCTS LLGALFPLP RIIYAMSND GLIFKFLAK 
INPKTKTPI IATVLSGIL VGAMAILFD TDQLISMMS 
IGTLLAYTI VAVCVLILR YQPTETQYP NLELKQNLE 
DSYTLFGMF KQLFNLNMT KYASSSSAK ITNWSIILF 
AVFTAAFDA FIIYAIDVL DKPYYMTLF IIVTLVMLT 
LVVIIARQP VDEVKLSFK VPWVPFVPC LSIIINLYL 
MLELDKDTW IRFGVWLFI VLSEVLMKM GLRTITFGQ 
VLETLSRKK VVEFETGTK LAKVLTTLD LTALGVGST 
LGVGIYVLA GDVAKNNAG PAVTVSFFI AAVTSILAG 
LCYAEFGAR VPKAGSAYV YSYVCIGEL FAFIIGWNL 
ILEYLIGSA TVVKALFLY LDELSNNVM SEFFQENIP 
IEAGGELGQ YADIFSLGL SFVFAVAIA LGAKESTLV 
NNIFTLVNL LVVATVVIS GLWKAKASN WSIPPEDVP 
SGSGTGGFA PFGIKGVIQ GAARCFFAF IGFDCIATA 
GEEAKTPSK SIPIGVVTS LLIVFFSYF GISTILTMM 
LPYYEQHEK APLTHIYDV VGWPALKYV VSVGAICGL 
FSSLLGAMF PLPRIIYAM ASDGLLFKA LAIVHPKFQ 
TPFMGTLIA GSIAGCLAC IFDVDKLAN MMSIGTLLA 
YSMVAACVL ILRYAVDEC EKKFEDKEE LTGTMYFKQ 
MFNRKSQFP TRLTSSLVS WLVLAYFSV SFIFSGLIT 
GFEKELENA EPWLLSLLC ILGVVLIVL LSIISWQPK 
SSVQLTFAV PLVPWIPGI SIVVNVYLM TTLPAHTWE 
YYGYWMLIG FLIYFAYGI WNSTERKSS KCSGTNNET 
LPDDIKQNH L
    CDS sequence
    • >TC034265 RA
      ttagaaaga ttgtgttat gcagaattt gctgctcga 
gtccctaag gcaggttct gcttacgtc tacagttac 
gtttcagtg ggtgaagtt gtcgcatat gtaattgga 
tggaattta atcttggaa tatgccata ggaaccgca 
agtgtagct agaggtcta agcggttac atagacgct 
cttgccaat caagatata aagaatttt atggaaagt 
tgggcaaaa atggacgtt ggattttta gcatcgtat 
cccgatttt ctagcgttt ggatttgtt gttcttctc 
acagctcta ctttcaatc ggcgtgaaa gaatcttct 
cgcctcaac aacatattc actggccta aatttgatc 
actgtgggc atcgttata gtatctgga gccatcaaa 
gccgactca tccaactgg aacaaaaaa ctcgaagac 
attccagac aaatttaga gaagatgcc ggaactggt 
ggttttatg ccttttgga gtctccgga atgatggaa 
ggcgcagct caatgtttc tacggtttc gtgggtttc 
gacgctgtt gccacaacc ggcgaagag gcgaaaaac 
ccccagaga aacatccct ctagccatc gtcatttcc 
cttcttgtg atctttctg gcctatttt ggcatttcg 
acagttttg acaatgatg tggccctat tacgaccaa 
gacccaact gctcctttt ccctatgtt ttcgaccaa 
ttgggttgg cctgccatt aagtggatt gtaacaatt 
ggggcagtt attgccctt tgtacgagt ctccttggt 
gcgttgttt cctcttcct aggattatc tatgctatg 
tcgaatgat gggttaatt ttcaaattt ttggccaaa 
attaatcca aaaactaaa actccaatt attgcaact 
gttctttcc ggaattcta gtaggtgca atggctata 
cttttcgat actgaccaa ttgattagt atgatgtca 
attgggact cttcttgcc tacactatc gttgcagtt 
tgtgttcta atactgagg taccaaccc acagaaacg 
caataccct aatctcgaa cttaagcaa aaccttgaa 
gacagttat acgttgttt ggaatgttt aaacaactc 
ttcaacttg aacatgacc aagtatgcc agttcaagc 
tccgccaaa atcacaaat tggagtata atcctgttt 
gctgttttc acagctgcc ttcgatgct ttcattatt 
tacgccatt gatgttctc gataaacct tattacatg 
acacttttt atcattgtt acgttagtt atgttgacc 
cttgttgtg attattgca agacaaccg gtcgacgaa 
gtcaaactc tcgtttaaa gttccttgg gtgcctttt 
gttccttgt ttgagtatc atcattaat ttgtatctt 
atgttggag ttggataag gatacttgg atacgattt 
ggtgtctgg ttgtttatt gtgctaagt gaagtttta 
atgaaaatg ggtctgcgc actatcaca tttggtcaa 
gtgctggaa actctttcg aggaagaaa gttgtagaa 
ttcgaaaca ggaacaaag ttggcaaaa gtgctcaca 
actttggat ttgactgcc ttgggagtg ggtagtact 
ttgggggtg ggtatatac gtactagct ggagatgtc 
gccaaaaat aacgcggga cctgctgta acagtgtcg 
ttttttatt gcagccgta acttcaatt cttgccggg 
ctttgttat gctgaattt ggggcaagg gtgcccaaa 
gctggctct gcctatgtt tacagttat gtgtgtata 
ggagagtta ttcgcattt atcattgga tggaatttg 
atactggaa tatctgata ggttctgca actgttgtc 
aaagcgcta tttctttac ttagacgaa ctatcaaac 
aacgtaatg tctgagttt tttcaagaa aatattccc 
atcgaagcg ggaggagaa ctaggacaa tacgctgac 
attttttct ttgggtcta tccttcgtt tttgctgtt 
gccatagca ttaggagcg aaagaatct accttagtt 
aataacata ttcactcta gtcaattta cttgtcgtt 
gccactgtt gtaatctcc ggattatgg aaagccaaa 
gcttccaac tggtctatt ccaccagag gatgtccct 
tcagggagt ggcactggc ggttttgct ccttttggc 
atcaaaggt gtgatccaa ggagccgcc agatgtttc 
tttgctttc attggcttc gattgtatt gccacagca 
ggtgaagag gcaaaaact ccaagcaaa tctattccc 
ataggtgtg gtaacatca cttctcatc gtgtttttt 
tcctacttt ggcatttcg acaatactt acaatgatg 
ctaccatac tatgaacaa cacgagaaa gctcccttg 
acccatatt tacgatgtg gttggttgg cctgccttg 
aaatacgtg gtcagtgtt ggtgcaatt tgtggcctt 
ttttcgagt ttattagga gctatgttt cctttaccc 
aggattatt tacgccatg gcttccgat ggtctactt 
ttcaaagct ttggctatt gttcatccc aagtttcag 
acacctttt atggggact ttaattgct ggaagtatc 
gcaggctgc ttggcttgt atttttgac gttgataag 
ttggccaac atgatgtct attggaaca cttttggca 
tattccatg gtagcagct tgtgttctt atcctgagg 
tatgctgtt gacgaatgt gagaagaaa tttgaagac 
aaggaagaa ttgactgga acaatgtat ttcaagcaa 
atgtttaac agaaaaagt caatttccg acaaggtta 
acttcatct ttagtttct tggctcgtt ttggcgtat 
ttttcagtt tcttttatt ttctctgga cttatcact 
ggtttcgaa aaagaattg gaaaatgcc gaaccttgg 
cttctttcc cttctttgt atacttggt gtggtttta 
attgttttg ttatccata atttcgtgg caacccaag 
tcttcagta caactaaca tttgcagtt cctttagtt 
ccttggatc cctggcatt agcatcgta gtcaatgtg 
tatttaatg acgactttg ccagctcac acttgggaa 
tattacggt tattggatg ctcataggt tttttgata 
tatttcgct tatggcatt tggaacagt accgaacga 
aaaagtagt aagtgttcc ggaacgaac aacgaaact 
ttaccagac gatataaaa caaaaccat ttgtaa
    mRNA sequence
    • >TC034265 RA
      attagaaag attgtgtta tgcagaatt tgctgctcg 
agtccctaa ggcaggttc tgcttacgt ctacagtta 
cgtttcagt gggtgaagt tgtcgcata tgtaattgg 
atggaattt aatcttgga atatgccat aggaaccgc 
aagtgtagc tagaggtct aagcggtta catagacgc 
tcttgccaa tcaagatat aaagaattt tatggaaag 
ttgggcaaa aatggacgt tggattttt agcatcgta 
tcccgattt tctagcgtt tggatttgt tgttcttct 
cacagctct actttcaat cggcgtgaa agaatcttc 
tcgcctcaa caacatatt cactggcct aaatttgat 
cactgtggg catcgttat agtatctgg agccatcaa 
agccgactc atccaactg gaacaaaaa actcgaaga 
cattccaga caaatttag agaagatgc cggaactgg 
tggttttat gccttttgg agtctccgg aatgatgga 
aggcgcagc tcaatgttt ctacggttt cgtgggttt 
cgacgctgt tgccacaac cggcgaaga ggcgaaaaa 
cccccagag aaacatccc tctagccat cgtcatttc 
ccttcttgt gatctttct ggcctattt tggcatttc 
gacagtttt gacaatgat gtggcccta ttacgacca 
agacccaac tgctccttt tccctatgt tttcgacca 
attgggttg gcctgccat taagtggat tgtaacaat 
tggggcagt tattgccct ttgtacgag tctccttgg 
tgcgttgtt tcctcttcc taggattat ctatgctat 
gtcgaatga tgggttaat tttcaaatt tttggccaa 
aattaatcc aaaaactaa aactccaat tattgcaac 
tgttctttc cggaattct agtaggtgc aatggctat 
acttttcga tactgacca attgattag tatgatgtc 
aattgggac tcttcttgc ctacactat cgttgcagt 
ttgtgttct aatactgag gtaccaacc cacagaaac 
gcaataccc taatctcga acttaagca aaaccttga 
agacagtta tacgttgtt tggaatgtt taaacaact 
cttcaactt gaacatgac caagtatgc cagttcaag 
ctccgccaa aatcacaaa ttggagtat aatcctgtt 
tgctgtttt cacagctgc cttcgatgc tttcattat 
ttacgccat tgatgttct cgataaacc ttattacat 
gacactttt tatcattgt tacgttagt tatgttgac 
ccttgttgt gattattgc aagacaacc ggtcgacga 
agtcaaact ctcgtttaa agttccttg ggtgccttt 
tgttccttg tttgagtat catcattaa tttgtatct 
tatgttgga gttggataa ggatacttg gatacgatt 
tggtgtctg gttgtttat tgtgctaag tgaagtttt 
aatgaaaat gggtctgcg cactatcac atttggtca 
agtgctgga aactctttc gaggaagaa agttgtaga 
attcgaaac aggaacaaa gttggcaaa agtgctcac 
aactttgga tttgactgc cttgggagt gggtagtac 
tttgggggt gggtatata cgtactagc tggagatgt 
cgccaaaaa taacgcggg acctgctgt aacagtgtc 
gttttttat tgcagccgt aacttcaat tcttgccgg 
gctttgtta tgctgaatt tggggcaag ggtgcccaa 
agctggctc tgcctatgt ttacagtta tgtgtgtat 
aggagagtt attcgcatt tatcattgg atggaattt 
gatactgga atatctgat aggttctgc aactgttgt 
caaagcgct atttcttta cttagacga actatcaaa 
caacgtaat gtctgagtt ttttcaaga aaatattcc 
catcgaagc gggaggaga actaggaca atacgctga 
cattttttc tttgggtct atccttcgt ttttgctgt 
tgccatagc attaggagc gaaagaatc taccttagt 
taataacat attcactct agtcaattt acttgtcgt 
tgccactgt tgtaatctc cggattatg gaaagccaa 
agcttccaa ctggtctat tccaccaga ggatgtccc 
ttcagggag tggcactgg cggttttgc tccttttgg 
catcaaagg tgtgatcca aggagccgc cagatgttt 
ctttgcttt cattggctt cgattgtat tgccacagc 
aggtgaaga ggcaaaaac tccaagcaa atctattcc 
cataggtgt ggtaacatc acttctcat cgtgttttt 
ttcctactt tggcatttc gacaatact tacaatgat 
gctaccata ctatgaaca acacgagaa agctccctt 
gacccatat ttacgatgt ggttggttg gcctgcctt 
gaaatacgt ggtcagtgt tggtgcaat ttgtggcct 
tttttcgag tttattagg agctatgtt tcctttacc 
caggattat ttacgccat ggcttccga tggtctact 
tttcaaagc tttggctat tgttcatcc caagtttca 
gacaccttt tatggggac tttaattgc tggaagtat 
cgcaggctg cttggcttg tatttttga cgttgataa 
gttggccaa catgatgtc tattggaac acttttggc 
atattccat ggtagcagc ttgtgttct tatcctgag 
gtatgctgt tgacgaatg tgagaagaa atttgaaga 
caaggaaga attgactgg aacaatgta tttcaagca 
aatgtttaa cagaaaaag tcaatttcc gacaaggtt 
aacttcatc tttagtttc ttggctcgt tttggcgta 
tttttcagt ttcttttat tttctctgg acttatcac 
tggtttcga aaaagaatt ggaaaatgc cgaaccttg 
gcttctttc ccttctttg tatacttgg tgtggtttt 
aattgtttt gttatccat aatttcgtg gcaacccaa 
gtcttcagt acaactaac atttgcagt tcctttagt 
tccttggat ccctggcat tagcatcgt agtcaatgt 
gtatttaat gacgacttt gccagctca cacttggga 
atattacgg ttattggat gctcatagg ttttttgat 
atatttcgc ttatggcat ttggaacag taccgaacg 
aaaaagtag taagtgttc cggaacgaa caacgaaac 
tttaccaga cgatataaa acaaaacca tttgtaaac 
ctcattcca acataattt atttattac caaaacaca 
aaacaataa agacaacaa accaaa
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