Status
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Gene information
General information
TC001933 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1203284..1210584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001933
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00313

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001933 RA
      MTSRKKSRH EPPFMNNIL DEIFHPEKP VCQIIPTGC 
      HKKQDLFTQ YRPQPKTCL DYNISQTDT KIDAGDTKI 
      HYQQTVAEY IPGLSDPPP PPPPKKRRY FSGGPPQWE 
      FMDEIWRRE QEKIQQYYI EVFQGFGKR TRNNHRYEM 
      MNSHRKAIA MVGPAWFQE LSPLQLVTV DNLKKCILK 
      DLADDTIIN TQENIAYLG LVLRPNHNH IAKALKHCC 
      KCPVEFLLI LYQLINPKR VKYSINDRL LLSAVVHLT 
      MQDTLKELH IRIPSPPRP PKQPKQVPK VKKSQKYDS 
      PYLVRFTFE REPPKFTGK YYNKHVQRP QSPYFCYLK 
      ELRKMQEGT LQVVANDKE IEEELILAQ SIYDDLVNF 
      KPVPKLLKP SKFVPCVNF RYKQQLKAE MDEREEEVK 
      EVQDDAPCY LEYVHAETE TEEIHGECV CPPTNTCPC 
      ALKDQSVQC GSKKIAIEE CHEETTCSC EEPVKKECL 
      FESSTVFDS SANSDFASI RAQYGSVKR KSVESEPKN 
      TCKHAEDVA RFEWYCEYK NRKPEEKKS CRCKQKYRE 
      VVEKPFCQC EQCQDERRK KGTIYGIAG MKETESDAV 
      IPIIDQVLA QKPCDCLKK YEARIDQFE KEKKKPCSC 
      RREAQKLIE TPFCQCDKC KEDRKQKAE KFIIGGLKE 
      TTEDNVPII EGITSERPC ICVKQYDEK ADKYEEFQK 
      RKQLVCSLK QQQDKYIID GVISTPEGP MYVISGMRP 
      PIDCACAKN ARKEEEEKR MEAIMPHAP GGRIRYGIT 
      GVKETPTGN VYILDSALP TEECDCMAV YQQFEDEHF 
      PCMGLYERY LAQTKDAYN EFMKEMLPP SSRRSSVTS 
      SVFRQSVVE GVENVKLEP VEEEEKQSE VSSPKSEPC 
      VKESKLDQA IEPCEKKSH PCAKEENIE PREKKSKLC 
      SACSQSYYA ACCSCGQVE CKDCTCGKV IECVDCTCG 
      PEEVEEEEV VEEEEEEQE LKRFFIFDK ILCNRRWQK 
      KVLKKALQS MADDGYPLA KLPECYKLP HFKLWMQMR 
      CGKYWTQDD KYNYVFKSR ILWRHCDVC NVRRNPSPK 
      MPITLEEAH KMNWSQAPA IRNMANVAI ERFYRTVKQ 
      HRVNFGREF FPTTFSYEF PFSTWRDCY FAYTPSKEE 
      DVLARFVWQ KYDFKNFAD MQRACEKPC DFLKKYKAR 
      INQFEKEKK KPCSCRREA QKLIETPFC QCDKCKEDR 
      KQKAEKFVI GGIKETTED NVPINEGIT SERPCICVK 
      QYDEKADKY EEFQKRKQL VCSLKQQQD KYIIDGVVS 
      TPEGPMYVI SGMRPPIDC ACAKNARKG EEEKRMEAI 
      MPHAPGGRI RYGITGVKE TPTGNVYIL DSALPTEEC 
      DCMAVYQQF EDEHFPCMG LYERYLAQT KDAYNEFMK 
      EMLPPSSRR SSVTSSVFR QSVVEGVEN VKLEPVEEE 
      EKQSEVASP KSEPCVKES KPDEAIEPC EKKSNLCAK 
      EENIEPGEK KSKLCSACS QSYYAACCS CGQVECKDC 
      TCGKVIECV DCTCGPEEV EEEEVVEEE EEEQELKRF 
      FIFDKILCN RRWQKKILK KALQSMADD GYPLAKLPE 
      CYKLPHFKL WMQMRCGKY WTQDDKYNY VFKSRILWR 
      HCDVCNVRR NPSPKMPIT LEEAHKMNW SQAPAIRNM 
      ANVAIERFY RTVKQHRVN FGREFFPTT FSYEFPFTT 
      WRDCYFAYT PSKEEDVLA RFVWQKYDF KNFADMQRA 
      CG
    CDS sequence
    • >TC001933 RA
      atgacttcg cgtaagaaa agtcgtcat gagccccct 
      tttatgaac aacatcttg gacgagatt tttcaccct 
      gaaaaacca gtttgccaa atcattcca acaggttgc 
      cacaaaaaa caagacctt ttcacccaa tacagaccc 
      cagccgaaa acttgtctg gattacaac atttcacaa 
      acggataca aaaatcgac gctggagac accaaaatt 
      cactaccaa caaactgta gccgagtac attccaggt 
      ctttcggac cctccacca cccccaccc ccaaaaaaa 
      cggcggtat tttagtggt gggcctccc cagtgggag 
      tttatggat gaaatatgg agacgagaa caagagaaa 
      atacaacaa tattatatt gaagtattt caagggttt 
      gggaaacgg accaggaat aatcatcga tacgagatg 
      atgaacagt caccgtaaa gctatagca atggttgga 
      cctgcctgg ttccaggaa ctttctccc ttacagttg 
      gtcacagtt gataatttg aaaaaatgt attcttaag 
      gatttggcc gatgatacg attatcaac acacaggaa 
      aatatcgca taccttggt ctagtttta cggccaaat 
      cataaccac atagccaaa gctttgaaa cattgttgt 
      aaatgtcca gttgagttt cttctaatt ttgtaccaa 
      ttgattaat cccaaacga gtgaaatat tcgataaat 
      gaccgactt ttgctaagt gctgtggtc cacctaacc 
      atgcaagac accctaaag gagttacat atcagaata 
      ccatcacca ccaagacca ccaaaacaa ccaaaacaa 
      gtaccaaaa gtaaagaag tcgcaaaaa tacgactca 
      ccatatctt gttcgtttc acttttgaa cgagaacca 
      ccaaagttc acaggaaag tactacaac aagcacgtt 
      caacgaccc caaagtcct tacttttgt tatttgaaa 
      gaactgagg aaaatgcaa gaagggacg ttacaagtt 
      gttgcaaac gacaaagaa atcgaagaa gagttgatt 
      cttgcccaa tcaatttat gacgatttg gttaatttt 
      aaaccagtc cctaaacta ctgaaacca tccaaattt 
      gtcccgtgt gtcaacttt aggtacaaa caacaactc 
      aaagctgaa atggatgaa cgagaagaa gaagtaaaa 
      gaagttcaa gatgatgct ccttgttat ttggaatat 
      gtccatgcg gagacagaa actgaagaa attcacggt 
      gaatgtgtg tgtccacct accaacact tgtccttgt 
      gccttgaaa gaccaatca gtacaatgt ggttcgaag 
      aaaattgca attgaagaa tgtcatgaa gaaacaact 
      tgctcgtgt gaagaaccg gtcaaaaaa gaatgtctt 
      tttgagagt agtaccgtt tttgatagt tcggccaac 
      agcgatttt gcgtcgatt agagcacaa tatggtagc 
      gtgaaacgt aaaagtgta gaaagtgaa ccaaaaaac 
      acgtgtaaa catgctgaa gatgtggca cgtttcgag 
      tggtattgt gagtacaaa aatcgtaaa cctgaagag 
      aaaaaatcg tgtcgttgc aaacagaaa tatagagaa 
      gttgttgaa aaaccgttt tgtcagtgc gaacaatgt 
      caggacgaa agaaggaag aaaggaaca atttatgga 
      attgctgga atgaaggaa acagaatcg gacgccgta 
      atcccgatc attgaccaa gtccttgca caaaaacca 
      tgcgattgt ttgaagaaa tacgaagct agaatcgac 
      caattcgaa aaagaaaag aagaaacct tgtagttgt 
      cgcagagaa gcacaaaaa ttgatcgaa acaccgttt 
      tgtcaatgt gacaaatgt aaagaagat cggaaacag 
      aaagctgag aagtttatt attggagga ctcaaggaa 
      acgactgaa gacaatgtt ccaatcatt gaaggtatt 
      acaagtgaa cgcccttgc atttgtgtg aagcaatac 
      gacgaaaag gccgataaa tacgaagag ttccaaaaa 
      cgcaaacaa ttagtttgt tcgttaaag caacaacaa 
      gacaaatat atcattgat ggtgttatt agtactcct 
      gaaggtcca atgtatgtc atttcgggg atgcgaccc 
      ccaattgat tgtgcttgt gctaaaaac gctcgcaag 
      gaagaagag gaaaaacgc atggaggcc ataatgcca 
      catgcacca ggtggaaga atccgatat gggattact 
      ggtgttaag gaaacaccc acaggaaat gtgtatatc 
      cttgatagt gctttaccg accgaagag tgtgactgt 
      atggctgtt taccaacaa ttcgaagac gaacatttt 
      ccttgtatg ggcttgtac gaaagatat ttggcacaa 
      acgaaagat gcatacaat gagtttatg aaggaaatg 
      ttgcctcca agtagtaga cgatcaagc gtgacgagt 
      tcggtcttt cgccaaagt gttgtggaa ggtgttgaa 
      aacgtaaag cttgaaccg gttgaagaa gaagaaaaa 
      caaagcgaa gtttcttca ccgaaatct gagccatgt 
      gtgaaggaa tccaaactg gaccaagca attgaacca 
      tgtgaaaaa aaatcacat ccatgtgca aaagaagaa 
      aacattgaa ccaagagaa aagaaatca aagttatgt 
      tcagcttgt tctcagagt tattatgct gcgtgttgc 
      agttgtggt caagtcgaa tgtaaagac tgcacgtgt 
      ggaaaagta atcgagtgt gttgattgc acgtgtggt 
      cccgaagaa gtcgaagaa gaggaagtt gttgaagag 
      gaggaagag gaacaggaa ttgaaacga ttcttcatt 
      tttgacaaa attttgtgc aatcgtagg tggcaaaag 
      aaagttctg aaaaaagct ttacaaagt atggccgat 
      gatggttac cctttagct aaacttcca gaatgctac 
      aagttacca cacttcaag ctttggatg caaatgaga 
      tgcggcaaa tattggact caggacgat aaatataac 
      tatgtcttt aagagccga atcttatgg aggcattgc 
      gatgtgtgc aatgtcaga cgtaatccg tctccaaaa 
      atgccgatc actcttgag gaagctcat aagatgaat 
      tggtcacaa gcacctgct atcagaaac atggcaaat 
      gttgcaatt gagagattt tatcgcact gtgaaacaa 
      cacagagtc aattttggc cgcgagttt ttccccaca 
      actttttcc tacgaattt cctttttcg acgtggaga 
      gactgttat tttgcttac actccttca aaggaagaa 
      gatgttcta gcacgattt gtgtggcaa aagtacgat 
      tttaaaaat tttgccgac atgcaaagg gcttgtgaa 
      aaaccatgc gattttttg aagaaatac aaagctagg 
      atcaaccaa tttgaaaaa gaaaagaag aaaccttgt 
      agttgtcgc agagaagca caaaaattg atcgaaacg 
      ccgttttgt caatgtgac aaatgtaaa gaagatcgg 
      aaacagaaa gcagagaaa tttgttatt ggaggaatc 
      aaggaaacg actgaagac aatgttcca atcaatgaa 
      ggtattaca agtgaacgc ccttgcatt tgtgtgaag 
      caatacgac gaaaaggcc gataaatac gaagagttc 
      caaaaacgc aaacaatta gtttgttcg ttgaagcaa 
      caacaagac aaatatatc attgatggt gttgttagt 
      actcctgaa ggtccaatg tatgtcatt tcggggatg 
      cgaccccca attgattgt gcttgtgct aaaaacgct 
      cgcaagggg gaagaggaa aaacgcatg gaggccata 
      atgccacat gcaccaggt ggaagaatc cgatatggg 
      attactggt gttaaggaa acacccacc ggaaatgtg 
      tatattctt gatagtgct ttaccgacc gaagagtgt 
      gactgtatg gctgtttac caacaattc gaagacgaa 
      cattttcct tgtatgggc ttgtacgag agatatttg 
      gcacaaacg aaagatgca tacaatgag tttatgaag 
      gaaatgttg cctccaagc agtagacga tcaagcgtg 
      acgagttcg gtctttcgc caaagtgtt gtggaaggt 
      gttgaaaac gtaaagctt gaaccggtt gaagaagaa 
      gaaaaacaa agtgaagtt gcttcaccg aaatctgag 
      ccatgtgta aaggaatcc aaaccggac gaagcaatt 
      gaaccatgt gaaaaaaaa tcaaatcta tgtgcaaaa 
      gaagaaaac attgaacca ggagaaaag aaatcaaag 
      ttatgttca gcttgttct cagagttat tatgctgcg 
      tgttgcagt tgtggtcaa gtcgaatgt aaagactgc 
      acgtgtgga aaagtaatc gagtgtgtt gattgcacg 
      tgtggtccc gaagaagtc gaagaagag gaagttgtt 
      gaagaggag gaagaggaa caggaattg aaacgattc 
      tttatattt gacaaaatt ttgtgcaat cgtaggtgg 
      caaaagaaa attctgaaa aaagcttta caaagtatg 
      gccgatgat ggttaccct ttagctaaa cttccagaa 
      tgttacaag ttaccacac ttcaagctt tggatgcaa 
      atgaggtgc ggcaaatat tggactcag gacgataaa 
      tataactat gtcttcaag agccgaatc ttatggagg 
      cattgcgat gtgtgcaat gtcagacgt aatccgtct 
      ccaaaaatg ccgatcact cttgaggaa gctcataag 
      atgaattgg tcccaagca cctgctatc agaaacatg 
      gcaaatgtt gcaattgag agattttat cgcactgtg 
      aaacagcac agagtcaat tttggccgc gagtttttc 
      cccacaact ttttcctac gaatttcct ttcacgacg 
      tggagagac tgttatttt gcttacact ccttcaaag 
      gaagaagat gttttggca cgatttgtg tggcaaaag 
      tacgatttt aaaaatttt gccgacatg caaagggct 
      tgtggttaa
    mRNA sequence
    • >TC001933 RA
      ttttcggtg tcaaaattt cgaatgctc taaattcta 
      aaactccac gatgacttc gcgtaagaa aagtcgtca 
      tgagccccc ttttatgaa caacatctt ggacgagat 
      ttttcaccc tgaaaaacc agtttgcca aatcattcc 
      aacaggttg ccacaaaaa acaagacct tttcaccca 
      atacagacc ccagccgaa aacttgtct ggattacaa 
      catttcaca aacggatac aaaaatcga cgctggaga 
      caccaaaat tcactacca acaaactgt agccgagta 
      cattccagg tctttcgga ccctccacc acccccacc 
      cccaaaaaa acggcggta ttttagtgg tgggcctcc 
      ccagtggga gtttatgga tgaaatatg gagacgaga 
      acaagagaa aatacaaca atattatat tgaagtatt 
      tcaagggtt tgggaaacg gaccaggaa taatcatcg 
      atacgagat gatgaacag tcaccgtaa agctatagc 
      aatggttgg acctgcctg gttccagga actttctcc 
      cttacagtt ggtcacagt tgataattt gaaaaaatg 
      tattcttaa ggatttggc cgatgatac gattatcaa 
      cacacagga aaatatcgc ataccttgg tctagtttt 
      acggccaaa tcataacca catagccaa agctttgaa 
      acattgttg taaatgtcc agttgagtt tcttctaat 
      tttgtacca attgattaa tcccaaacg agtgaaata 
      ttcgataaa tgaccgact tttgctaag tgctgtggt 
      ccacctaac catgcaaga caccctaaa ggagttaca 
      tatcagaat accatcacc accaagacc accaaaaca 
      accaaaaca agtaccaaa agtaaagaa gtcgcaaaa 
      atacgactc accatatct tgttcgttt cacttttga 
      acgagaacc accaaagtt cacaggaaa gtactacaa 
      caagcacgt tcaacgacc ccaaagtcc ttacttttg 
      ttatttgaa agaactgag gaaaatgca agaagggac 
      gttacaagt tgttgcaaa cgacaaaga aatcgaaga 
      agagttgat tcttgccca atcaattta tgacgattt 
      ggttaattt taaaccagt ccctaaact actgaaacc 
      atccaaatt tgtcccgtg tgtcaactt taggtacaa 
      acaacaact caaagctga aatggatga acgagaaga 
      agaagtaaa agaagttca agatgatgc tccttgtta 
      tttggaata tgtccatgc ggagacaga aactgaaga 
      aattcacgg tgaatgtgt gtgtccacc taccaacac 
      ttgtccttg tgccttgaa agaccaatc agtacaatg 
      tggttcgaa gaaaattgc aattgaaga atgtcatga 
      agaaacaac ttgctcgtg tgaagaacc ggtcaaaaa 
      agaatgtct ttttgagag tagtaccgt ttttgatag 
      ttcggccaa cagcgattt tgcgtcgat tagagcaca 
      atatggtag cgtgaaacg taaaagtgt agaaagtga 
      accaaaaaa cacgtgtaa acatgctga agatgtggc 
      acgtttcga gtggtattg tgagtacaa aaatcgtaa 
      acctgaaga gaaaaaatc gtgtcgttg caaacagaa 
      atatagaga agttgttga aaaaccgtt ttgtcagtg 
      cgaacaatg tcaggacga aagaaggaa gaaaggaac 
      aatttatgg aattgctgg aatgaagga aacagaatc 
      ggacgccgt aatcccgat cattgacca agtccttgc 
      acaaaaacc atgcgattg tttgaagaa atacgaagc 
      tagaatcga ccaattcga aaaagaaaa gaagaaacc 
      ttgtagttg tcgcagaga agcacaaaa attgatcga 
      aacaccgtt ttgtcaatg tgacaaatg taaagaaga 
      tcggaaaca gaaagctga gaagtttat tattggagg 
      actcaagga aacgactga agacaatgt tccaatcat 
      tgaaggtat tacaagtga acgcccttg catttgtgt 
      gaagcaata cgacgaaaa ggccgataa atacgaaga 
      gttccaaaa acgcaaaca attagtttg ttcgttaaa 
      gcaacaaca agacaaata tatcattga tggtgttat 
      tagtactcc tgaaggtcc aatgtatgt catttcggg 
      gatgcgacc cccaattga ttgtgcttg tgctaaaaa 
      cgctcgcaa ggaagaaga ggaaaaacg catggaggc 
      cataatgcc acatgcacc aggtggaag aatccgata 
      tgggattac tggtgttaa ggaaacacc cacaggaaa 
      tgtgtatat ccttgatag tgctttacc gaccgaaga 
      gtgtgactg tatggctgt ttaccaaca attcgaaga 
      cgaacattt tccttgtat gggcttgta cgaaagata 
      tttggcaca aacgaaaga tgcatacaa tgagtttat 
      gaaggaaat gttgcctcc aagtagtag acgatcaag 
      cgtgacgag ttcggtctt tcgccaaag tgttgtgga 
      aggtgttga aaacgtaaa gcttgaacc ggttgaaga 
      agaagaaaa acaaagcga agtttcttc accgaaatc 
      tgagccatg tgtgaagga atccaaact ggaccaagc 
      aattgaacc atgtgaaaa aaaatcaca tccatgtgc 
      aaaagaaga aaacattga accaagaga aaagaaatc 
      aaagttatg ttcagcttg ttctcagag ttattatgc 
      tgcgtgttg cagttgtgg tcaagtcga atgtaaaga 
      ctgcacgtg tggaaaagt aatcgagtg tgttgattg 
      cacgtgtgg tcccgaaga agtcgaaga agaggaagt 
      tgttgaaga ggaggaaga ggaacagga attgaaacg 
      attcttcat ttttgacaa aattttgtg caatcgtag 
      gtggcaaaa gaaagttct gaaaaaagc tttacaaag 
      tatggccga tgatggtta ccctttagc taaacttcc 
      agaatgcta caagttacc acacttcaa gctttggat 
      gcaaatgag atgcggcaa atattggac tcaggacga 
      taaatataa ctatgtctt taagagccg aatcttatg 
      gaggcattg cgatgtgtg caatgtcag acgtaatcc 
      gtctccaaa aatgccgat cactcttga ggaagctca 
      taagatgaa ttggtcaca agcacctgc tatcagaaa 
      catggcaaa tgttgcaat tgagagatt ttatcgcac 
      tgtgaaaca acacagagt caattttgg ccgcgagtt 
      tttccccac aactttttc ctacgaatt tcctttttc 
      gacgtggag agactgtta ttttgctta cactccttc 
      aaaggaaga agatgttct agcacgatt tgtgtggca 
      aaagtacga ttttaaaaa ttttgccga catgcaaag 
      ggcttgtga aaaaccatg cgatttttt gaagaaata 
      caaagctag gatcaacca atttgaaaa agaaaagaa 
      gaaaccttg tagttgtcg cagagaagc acaaaaatt 
      gatcgaaac gccgttttg tcaatgtga caaatgtaa 
      agaagatcg gaaacagaa agcagagaa atttgttat 
      tggaggaat caaggaaac gactgaaga caatgttcc 
      aatcaatga aggtattac aagtgaacg cccttgcat 
      ttgtgtgaa gcaatacga cgaaaaggc cgataaata 
      cgaagagtt ccaaaaacg caaacaatt agtttgttc 
      gttgaagca acaacaaga caaatatat cattgatgg 
      tgttgttag tactcctga aggtccaat gtatgtcat 
      ttcggggat gcgaccccc aattgattg tgcttgtgc 
      taaaaacgc tcgcaaggg ggaagagga aaaacgcat 
      ggaggccat aatgccaca tgcaccagg tggaagaat 
      ccgatatgg gattactgg tgttaagga aacacccac 
      cggaaatgt gtatattct tgatagtgc tttaccgac 
      cgaagagtg tgactgtat ggctgttta ccaacaatt 
      cgaagacga acattttcc ttgtatggg cttgtacga 
      gagatattt ggcacaaac gaaagatgc atacaatga 
      gtttatgaa ggaaatgtt gcctccaag cagtagacg 
      atcaagcgt gacgagttc ggtctttcg ccaaagtgt 
      tgtggaagg tgttgaaaa cgtaaagct tgaaccggt 
      tgaagaaga agaaaaaca aagtgaagt tgcttcacc 
      gaaatctga gccatgtgt aaaggaatc caaaccgga 
      cgaagcaat tgaaccatg tgaaaaaaa atcaaatct 
      atgtgcaaa agaagaaaa cattgaacc aggagaaaa 
      gaaatcaaa gttatgttc agcttgttc tcagagtta 
      ttatgctgc gtgttgcag ttgtggtca agtcgaatg 
      taaagactg cacgtgtgg aaaagtaat cgagtgtgt 
      tgattgcac gtgtggtcc cgaagaagt cgaagaaga 
      ggaagttgt tgaagagga ggaagagga acaggaatt 
      gaaacgatt ctttatatt tgacaaaat tttgtgcaa 
      tcgtaggtg gcaaaagaa aattctgaa aaaagcttt 
      acaaagtat ggccgatga tggttaccc tttagctaa 
      acttccaga atgttacaa gttaccaca cttcaagct 
      ttggatgca aatgaggtg cggcaaata ttggactca 
      ggacgataa atataacta tgtcttcaa gagccgaat 
      cttatggag gcattgcga tgtgtgcaa tgtcagacg 
      taatccgtc tccaaaaat gccgatcac tcttgagga 
      agctcataa gatgaattg gtcccaagc acctgctat 
      cagaaacat ggcaaatgt tgcaattga gagatttta 
      tcgcactgt gaaacagca cagagtcaa ttttggccg 
      cgagttttt ccccacaac tttttccta cgaatttcc 
      tttcacgac gtggagaga ctgttattt tgcttacac 
      tccttcaaa ggaagaaga tgttttggc acgatttgt 
      gtggcaaaa gtacgattt taaaaattt tgccgacat 
      gcaaagggc ttgtggtta agtaataaa atggaattt 
      ggtttgtcc ccattgttt tgtgttttg gtaataaat 
      aaattatgt ggaaatga
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