Status
Please wait ...
Gene information
General information
TC034271
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001928,TC001926,TC001929,TC001927
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid63: 214484..233388
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034271
      iBeetle screen iB_00312
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034271 RA
        MKKESKRNL VASSKSAFT LETLPYVPL HKFCTKKLL 
        EIALVAPLT TNDQKFLDQ IIETGKQFE YLSDVHFIT 
        NTDVLDLVE RLFQKKHTD LPKLLKQCA EEFVLKQFQ 
        EISDLAVMT KLKIIECLY TKYSLEVKK MQRSQELHD 
        ELNALLKDC ETKEKKKGK TKGKKGKKD KSTKSNKSK 
        KSKSSQQET KPGKTKEKK GKKDKSTKS KKTKKSPNI 
        QQETKPERE KIVEDYTKI VLKDNEVTF DYNVDNHVT 
        YFVLSGFSD PLLFEELSK IDIGVSAII NVSLENSNE 
        TPSDFIVKL DNLFQTPKT SQFLENTIR MKYRSDGKE 
        PTKKNLEEL LRVVKKINY FKRQHLAYI RSLKLYDFG 
        SFRHVVLNG TEKYSRIMN YYPPDVVNI ALIVAALVQ 
        QICDELTPI SSISDLLQS PLGEKTYQV HEDDVLKMS 
        LETYKEVGR VCYDTTLDY LKLIQPLAP IESMSKGTR 
        LQNRAISTE DDCYCDNTK DLFDYFHLA MFNQMTRTK 
        FVPKTFVST VLESYNEAK QYQNSLPRG ELASEPDEN 
        EVIKHYHMK EELSTSVLR QLVFKAMNS YSCWRREFC 
        AESESILVK FHDNLDEFG IETKIWNQA LTTPICLRD 
        FCRFVAEER EEIDQEVEE IEECEGLSN LNVPELYQE 
        YHYLLGASS EQTMEEAKR IFNETIQEL VDYPNSHAL 
        RSYCSEDGF VAYDLGPKR CQLTGHTTI FRSDDGVKV 
        TIDTSRFMV EDPKCTITL TADDNSLIF HAERPSKFN 
        FVLRDGTIV NFLEFGQNF EDHQVKEEI DEKLSHDES 
        IPGEETQSS YNDLWLQIL RKSKIQETI PKFLDKRVQ 
        LDEGNLDRF LELFKEAVY TQRPIYKIS TKSSLRHLK 
        RKTSPVFVP LVSILRRVL FKPVRRKRK IGWSVLNIP 
        NEVKKGPLK YDFQITLPN GLFVSTLPK NRQVTEVKQ 
        GYNFNCYTE KYRIFYSNG VIVIKNCDG STTILRSNG 
        DIIELTDES ECYGNDCAR NMAQFYKNE DEETCPYRT 
        TRCSEAPKV KVRSDFVDL YEFGDIPYA RKSLLDFNG 
        KKVSVSDGL VTETQLYYV VQEHDYHAE EVFYEREDG 
        MKCVIDKKG KRIFQFPDG TKITSWPEF QNDLIQVST 
        NPKVEGWVS VVTNYKFEH PDYIAILYD GVQGSTQIF 
        LKDGVLKSS QESLTMTTK KNIALEVCS DCIIFLKTC 
        DTCDGDCIS KIHLDQNSK FLEVYDTYD KYLWCDSSG 
        RCETNSNYI KLGVNKKGC DHRWKNENQ QLFILNGRG 
        PPGTLLWKD SSIEAKIGE SKLIKSRVV NYMNKLTKK 
        QTLEFYTDV HQDFPGRFT MSQMYNHDI CLSKFKHSD 
        EKPLFYVTY RVLIEFQNV AEHPEIIAE LDEFLERNS 
        RGISIGLFE GIKTILNCV VEELKQDKC LYQVSMQEV 
        EMSDQTTSS LSKSLDLDR TTVQDIVAQ WKKKPKGTT 
        LQNRSISTE DDCYCHDTK DLFDYFHLA MFNQMTRTK 
        FVPKTFVST VLERYDEAK QYQNSLPRG EFASEPDEN 
        KVIKHYHMK EELSTSVLR QLVFKAMNS YSCWRREFC 
        EESESILVK FHDNLDDFG FETKIWNQA LTTPICLRD 
        FCRFVAEER EEIDQEDEE IEECEGLSN LNVPELYQE 
        YHYLLGTSS RQTMEETKR IFNETIQEL VDYPNSHAL 
        RSYCSEDGF VAYDLGPKR CQLTGHTTI FRSDDGVKV 
        TIDTSRFMV EDPKCTITL TADDNSLIF HAERPSKFN 
        FVLRDGTIV NFLEFGQNF EDHQVKEEI DEKLSHDES 
        ISGEETQSS YNDLWLQIL RKSKIQETF PKFWYKRVQ 
        LDEGNLDRF LELFKEAVY TQRPIYKIS TKSSLRHLK 
        RKTSPVFVP LVSILRRVL FKPIRRKRK IGWSVLNIP 
        NVMKKGPLK YDFQITLPN GLFVSTLPK NRQVTEVKQ 
        GYNFNCYTE KYRIFYSNG VIVIKNCDG STTILRSNG 
        DIIELTDES ECYGNDCAR NMAQFYKNE DEETCPYRT 
        TRCSEAPKV KVRSDFVDL YEFGDIPYA RKSLLDFNG 
        KKVSVSDGL VTETQLYYV VQEHDYHAE EVFYEREDG 
        MKCVIDKKG KRIFQFPDG TKITSWPEF QNDLIQVST 
        NPKVEGWVS VVTNYKFEH PDYIAILYD GVQGSTQIF 
        LKDGVLKSS QESLTMTTK KNIALEVCS DCIIFLKTC 
        DTCDGDCIS KIHLDQNSK LLEVYDTYD KYLWCDSSG 
        RCETNSNYI KLGVNKKGC DHRWKNENQ QLFILNGRG 
        PPGTLLWKD SSIEAKIGE SKLIKSRVV NYMNKLTKK 
        QTLEFYTDV HQDFPGRFT MSQMYNHDI CLSKFKHSD 
        EKPLFYVMY RVLIEFQNV AEHPEIIAE LDEFLERNS 
        RGISIGLFE GIKTILNCV VEELKQDKC LYQVSMQEV 
        EMSDQTTSS LSKSLDLDR TTVQDIVAQ WKKRPKGTT 
        LQNRSISTE DDCYCHNTK DLFDYFHLA MFNQMTRTK 
        FVPKTFVST VLERYNEAR QYQNSLPRD EFASESDEN 
        KVIKHYHMK EELSTSVLR QLVFKAMNS YSCWRREFC 
        EESESILVK FHDNLDDFG IETKIWNQA LTTPICLRD 
        FCRFVAEER GEIDQEDEE IEECEGLSN LNAPELYQE 
        YHYLLGASS EQTMEETKQ IFNETIQEL VDYPNSHAL 
        RSYCSEDGF VAYDLGPKR CQLTGHTTI FRSDDGVKV 
        TMIRQGSWL RTQNVPSP
      CDS sequence
      • >TC034271 RA
        atgaagaaa gagagcaaa cgaaacttg gtggcttct 
        tccaaatca gcgttcaca cttgaaacg ttaccttac 
        gtaccgctg cacaaattc tgcactaag aaattgctc 
        gaaatcgca ttggtggct ccactgaca accaatgat 
        caaaaattt ttggaccaa attatcgaa acggggaaa 
        caattcgag tatttgtcg gatgttcac ttcattacg 
        aacaccgat gtattggat ttggtcgaa cgtttgttc 
        cagaaaaaa cacaccgac ttgcctaaa ctcctgaaa 
        caatgtgcg gaagaattt gtcttgaag cagtttcaa 
        gaaataagt gatttagct gtgatgacc aaactcaaa 
        ataatcgag tgtttgtac acgaagtac agtttggag 
        gtgaagaaa atgcaacga agtcaggaa ttacatgat 
        gagctgaac gcattgctt aaagattgt gaaacgaag 
        gagaaaaaa aagggtaaa actaaggga aagaaagga 
        aaaaaggac aagtctacc aagagcaat aaatcaaaa 
        aagagcaaa agtagtcaa caagaaacc aaaccgggt 
        aaaactaag gagaagaaa ggaaaaaag gacaagtct 
        accaaaagc aaaaaaaca aaaaagagc ccaaacatt 
        caacaagaa accaaaccg gaacgagaa aaaatcgtc 
        gaagactac acaaaaatc gtcctcaaa gacaacgaa 
        gtcactttc gactacaac gtcgacaac cacgtcact 
        tatttcgtc ttgagcggt ttttcggac cctttgctg 
        ttcgaagag ttgtccaaa attgatatt ggagtgtcg 
        gcaatcatc aacgtttcg ttagaaaat tcaaacgaa 
        acaccttcg gattttatc gtcaagtta gacaatttg 
        tttcaaaca ccaaaaact tctcaattt ttggaaaac 
        accatacgg atgaagtac agaagcgac ggtaaagaa 
        ccgacgaag aagaacctt gaagagctt ttacgtgtg 
        gtcaagaaa attaattac ttcaaacgc caacatctg 
        gcctacatc aggtcgctc aaactgtat gattttggc 
        tcatttcgc catgttgtg ctcaacggt acggaaaaa 
        tatagtcga attatgaat tattacccg cctgatgtc 
        gtaaacatt gccctaatt gtggccgct ttggtgcaa 
        caaatctgt gacgaattg actccaatt tcgtcaatt 
        tccgacttg cttcagtca ccattgggc gaaaaaacc 
        tatcaagtg cacgaagac gacgtcctt aaaatgagc 
        ctcgagact tacaaagag gtcggaagg gtgtgttac 
        gacacaaca cttgactac ctcaaacta atccaaccc 
        ttggctcca attgaatca atgtccaaa gggaccaga 
        ctccaaaat cgagcaatt tcaaccgaa gatgactgt 
        tattgtgac aacaccaaa gacctgttc gattatttc 
        cacttggca atgttcaac caaatgaca cgaactaaa 
        tttgtgcca aaaactttt gtttcgaca gttttggaa 
        agctacaac gaagcaaaa caataccaa aattcgctc 
        cctcgaggc gaattggcg tcagaaccc gacgaaaac 
        gaagtcatc aaacactac cacatgaag gaggagtta 
        tcgacttcg gtccttcgc cagttggtt ttcaaagca 
        atgaacagt tactcctgt tggaggcga gaattttgt 
        gcagaaagc gagtcaatc cttgttaaa ttccacgac 
        aatttggac gaatttggt attgaaaca aagatctgg 
        aaccaggcg ttaacaaca ccgatttgt ttgcgcgat 
        ttttgccgc tttgtggcg gaagaaagg gaagaaatt 
        gatcaagag gtcgaagaa attgaagaa tgtgaaggt 
        ttaagcaac ttgaacgtg ccagagttg taccaagag 
        taccattat ttattgggt gcgtccagt gaacaaacg 
        atggaagaa gcgaaacga attttcaac gagacaatt 
        caagaattg gttgattat cccaatagt cacgctttg 
        aggagttat tgttctgaa gatggtttc gtcgcgtat 
        gatttgggg ccgaaacgt tgccaatta actggtcat 
        actacaatt tttcggagt gatgatggt gttaaagtt 
        acaatagat acgtcaagg ttcatggtt gaggaccca 
        aaatgtacc atcacgcta acagcagac gacaacagt 
        ttgattttt catgcggaa cgcccttcg aaatttaac 
        tttgttcta agggacggc actattgtc aatttcctg 
        gaatttggc caaaatttt gaagatcac caggtgaaa 
        gaagaaatt gacgaaaaa ctgagtcat gatgaatcg 
        attccaggc gaagagaca cagtcaagt tacaacgac 
        ctttggttg caaatttta cgtaaaagt aagattcaa 
        gaaacgatt ccaaaattt ttggacaaa agagtgcaa 
        ctagacgaa gggaattta gatcgtttc ttggaactt 
        ttcaaagag gcggtttac acccaaaga ccgatttac 
        aaaatatca acaaaatcg tcccttcgc cacttgaaa 
        cgtaagact tcgccggtc tttgtccca cttgttagt 
        atcttgaga cgtgtcttg ttcaaacca gttcgccgg 
        aagcgtaaa attggttgg agcgttctt aatatccct 
        aatgaagtg aaaaagggg cccttgaag tatgatttt 
        cagataacc cttcctaat ggattattt gttagtacc 
        cttccgaag aaccgtcaa gtgactgaa gtgaagcaa 
        gggtacaac tttaattgt tacacagag aagtaccga 
        attttttat tcgaatgga gtgattgtg atcaaaaat 
        tgcgacggt tcgacgaca attcttcgc tcaaacggc 
        gatataatt gaattaact gacgaaagc gaatgttat 
        ggtaatgac tgtgctcga aatatggct caattttac 
        aaaaacgaa gacgaagaa acgtgtcct tatcgaacg 
        actcgatgt tccgaagct ccgaaagtt aaagtgagg 
        agtgatttc gtcgatttg tacgaattt ggtgacatt 
        ccatacgcg aggaagagt ttattggac tttaacgga 
        aaaaaagtt tcggtttcc gatggtcta gtgaccgaa 
        acacaactc tattacgtc gtgcaagaa cacgattat 
        cacgctgag gaagtgttt tatgagagg gaggacggc 
        atgaagtgt gtgatcgat aaaaagggg aagcgaatc 
        ttccagttt cctgatggt actaaaata acatcatgg 
        cccgaattt caaaacgat ttgatccaa gtcagcaca 
        aaccccaaa gtggaaggg tgggtttcc gttgtgaca 
        aactacaaa tttgaacat ccagactat atcgcaatt 
        ctttacgat ggagttcag ggatcgact cagattttc 
        ctcaaagat ggtgttctt aaatcgagt caagaaagc 
        ttgacaatg accacgaag aaaaatata gcgttggag 
        gtttgctcc gactgtata atttttctc aaaacttgt 
        gacacgtgt gatggtgat tgtatctct aaaatacac 
        ctggaccaa aattccaaa tttttggaa gtgtatgac 
        acctacgac aagtacctc tggtgtgat tcttcagga 
        aggtgtgaa accaactct aattatatc aaattaggg 
        gttaataaa aaaggttgt gaccatcga tggaaaaac 
        gagaaccaa caactattc attttgaat ggtcgtggt 
        ccacctgga acccttctc tggaaagac agttcgata 
        gaagcaaaa atcggcgaa agcaagtta atcaaaagc 
        cgcgtcgta aattacatg aacaagttg accaaaaaa 
        caaactttg gagttttac actgatgtg caccaggat 
        tttccagga aggttcact atgtcgcaa atgtacaac 
        cacgacatt tgtttgtcg aaattcaaa cattcggac 
        gagaaacca ctattttac gttacgtat cgggttttg 
        atagagttt caaaacgtg gcagaacac ccggaaatc 
        attgcagag ttggatgag tttttggaa cggaacagt 
        agaggcatt tcgattggt ttgttcgaa ggaattaaa 
        acgattcta aattgtgtt gttgaggag ttgaaacaa 
        gacaagtgt ttgtatcag gtgagcatg caagaggtg 
        gaaatgtcc gaccagact acaagtagt ttgagcaaa 
        agcttagat ttggatcgg acgacggtt caagatatt 
        gtggcgcag tggaaaaaa aaacccaaa gggaccaca 
        ctccaaaat cggtcaatt tcgaccgaa gacgactgt 
        tattgtcat gataccaaa gacctgttc gattatttc 
        cacttggca atgttcaac caaatgaca cgaacgaaa 
        ttcgtgcca aaaactttc gtttcgaca gttttggaa 
        cgctacgac gaagcgaaa cagtaccaa aattcgctc 
        cctcgaggc gaatttgcg tcagaaccc gacgaaaac 
        aaagtcatc aaacactac cacatgaag gaggagtta 
        tcgacttcg gtccttcgc cagttggtt ttcaaagca 
        atgaacagt tactcctgt tggaggcgc gaattttgt 
        gaagaaagc gagtcaatc cttgttaaa ttccacgac 
        aatttggac gactttggt tttgaaaca aagatctgg 
        aaccaggcg ttaacaaca ccgatttgt ttgcgcgat 
        ttttgccgc tttgtggcg gaagaaagg gaagaaatt 
        gatcaagag gacgaagaa attgaagaa tgtgaaggt 
        ttaagcaac ttgaacgtg ccagaattg taccaagag 
        taccattat ttattgggt acgtctagt agacaaacg 
        atggaagaa acgaaacga attttcaac gagacaatt 
        caagaattg gttgattat cccaatagt cacgctttg 
        aggagttat tgttctgaa gatggtttc gtcgcgtat 
        gatttgggg ccgaaacgt tgccaatta actggtcat 
        actacaatt tttcggagt gatgatggt gttaaagtt 
        acaatagat acgtcaagg ttcatggtt gaggaccca 
        aaatgtacc atcacacta acagcagac gacaacagt 
        ttgattttt catgcggaa cggccttcg aaatttaat 
        tttgttcta agggacggc actattgtc aattttctg 
        gaatttggc caaaatttt gaagatcac caggtgaaa 
        gaagaaatt gacgaaaaa ctgagtcat gatgaatcg 
        atttcaggc gaagaaaca cagtcaagt tacaacgac 
        ctttggttg caaatttta cgtaaaagt aaaattcaa 
        gaaacgttt ccaaaattt tggtacaaa agggtgcaa 
        ctagacgaa gggaattta gatcgtttc ttggaactt 
        ttcaaagag gcggtttac acccaaaga ccgatttac 
        aaaatatca acaaaatcg tcccttcgc cacttgaaa 
        cgtaagact tcgccggtc tttgtccca cttgttagt 
        atcttgaga cgtgtcttg tttaaacca attcgccgg 
        aagcgtaaa attggttgg agcgtcctt aatatccct 
        aatgtaatg aaaaagggg cccttgaag tatgatttt 
        cagataacc cttcccaat ggattattt gttagtacc 
        cttccgaag aaccgtcaa gtgactgaa gtgaagcaa 
        gggtacaac tttaattgt tacacagag aagtaccga 
        attttttat tcgaatgga gtgattgtg atcaaaaat 
        tgcgacggt tcgacgaca attcttcgc tcaaacggc 
        gatataatc gaattaact gatgaaagt gaatgttat 
        ggtaatgac tgtgcgcga aatatggct caattttac 
        aaaaacgaa gacgaagaa acgtgtcct tatcgaacg 
        actcgttgt tccgaagct ccgaaagtt aaagtgagg 
        agtgatttc gtcgatttg tatgaattt ggtgacatt 
        ccatacgcg aggaagagt ttattggac tttaacgga 
        aaaaaagtt tcggtttcc gatggtcta gtgaccgaa 
        acacaactc tattacgtc gtgcaagag cacgattat 
        cacgctgag gaagtgttt tatgagagg gaagacggc 
        atgaagtgt gttattgat aaaaagggg aagcgaatc 
        ttccagttt cctgatggt actaaaata acatcatgg 
        cccgaattt caaaacgat ttgatccaa gtcagcaca 
        aaccccaag gtggaaggg tgggtttcc gttgtgaca 
        aattacaaa tttgaacat ccagactat atcgcaatt 
        ctttacgat ggagttcag ggatcgact cagattttc 
        ctcaaagat ggtgttctt aaatcgagt caagaaagc 
        ttgacaatg accacgaag aaaaatata gcgttggag 
        gtttgctcc gactgtata attttcctc aaaacttgt 
        gacacgtgt gatggtgat tgtatttct aaaatacac 
        ctggaccaa aactccaaa cttttggaa gtgtatgac 
        acttacgac aagtacctc tggtgtgat tcttcagga 
        aggtgtgaa accaactct aattatatc aaattaggg 
        gttaataaa aaaggttgt gaccatcga tggaaaaac 
        gagaaccaa caactattc attttgaat ggtcgtggt 
        ccacctgga acccttctc tggaaagac agttcgata 
        gaagcaaaa atcggcgaa agcaagtta atcaaaagc 
        cgcgtcgta aattacatg aacaagttg accaaaaaa 
        caaactttg gagttttac actgatgtg caccaggat 
        tttccagga aggttcact atgtcgcaa atgtacaac 
        cacgacatt tgcttgtcg aaattcaaa cattcggac 
        gagaaacca ctattttac gttatgtat cgggttttg 
        atagagttt caaaacgtg gcagaacac ccggaaatc 
        attgcagag ttggatgag tttttggaa cggaacagt 
        agaggcatt tcgattggt ttgttcgaa ggaattaaa 
        acgattcta aattgtgtt gttgaggag ttgaaacaa 
        gacaagtgt ttgtatcag gtgagcatg caagaggtg 
        gaaatgtcg gaccagact acaagtagt ttgagcaaa 
        agcttagat ttggatcgg acgacggtt caagatatt 
        gtggcgcag tggaaaaaa aggcccaag gggaccaca 
        ctccaaaat cggtcaatt tcgaccgaa gacgactgt 
        tattgtcat aataccaaa gacctgttc gattatttc 
        cacttggca atgttcaac caaatgaca cgaactaaa 
        tttgtgcca aaaactttt gtttcgaca gttttggaa 
        cgctacaac gaagcaaga caataccaa aattcgctc 
        cctcgagac gaatttgcg tcagaatcc gacgaaaac 
        aaagtcatc aaacactac cacatgaag gaggagtta 
        tcgacttcg gttcttcgc cagttggtt ttcaaagca 
        atgaacagt tactcctgt tggaggcga gaattttgc 
        gaagaaagc gagtcaatc cttgttaaa ttccacgac 
        aatttggac gattttggt attgaaaca aagatctgg 
        aaccaggca ttaacaaca ccgatttgt ttgcgcgat 
        ttttgccgc tttgtagct gaagaaagg ggagaaatt 
        gatcaagag gacgaagaa attgaagaa tgtgaaggt 
        ttgagcaac ttgaacgcg ccagaattg taccaagag 
        taccattat ttattgggt gcgtccagt gaacaaacg 
        atggaagaa acgaaacaa attttcaac gagacaatt 
        caagaattg gttgattat cccaatagt cacgctttg 
        aggagttat tgttctgaa gatggtttc gtcgcgtat 
        gatttaggg ccgaaacgt tgccaatta actggtcat 
        actacaatt tttcggagt gatgatggt gttaaagtt 
        acaatgata cgtcaaggt tcatggttg aggacccaa 
        aatgtacca tcaccctaa
      mRNA sequence
      • >TC034271 RA
        caattcaaa ccatttttg tcaaaaatg aagaaagag 
        agcaaacga aacttggtg gcttcttcc aaatcagcg 
        ttcacactt gaaacgtta ccttacgta ccgctgcac 
        aaattctgc actaagaaa ttgctcgaa atcgcattg 
        gtggctcca ctgacaacc aatgatcaa aaatttttg 
        gaccaaatt atcgaaacg gggaaacaa ttcgagtat 
        ttgtcggat gttcacttc attacgaac accgatgta 
        ttggatttg gtcgaacgt ttgttccag aaaaaacac 
        accgacttg cctaaactc ctgaaacaa tgtgcggaa 
        gaatttgtc ttgaagcag tttcaagaa ataagtgat 
        ttagctgtg atgaccaaa ctcaaaata atcgagtgt 
        ttgtacacg aagtacagt ttggaggtg aagaaaatg 
        caacgaagt caggaatta catgatgag ctgaacgca 
        ttgcttaaa gattgtgaa acgaaggag aaaaaaaag 
        ggtaaaact aagggaaag aaaggaaaa aaggacaag 
        tctaccaag agcaataaa tcaaaaaag agcaaaagt 
        agtcaacaa gaaaccaaa ccgggtaaa actaaggag 
        aagaaagga aaaaaggac aagtctacc aaaagcaaa 
        aaaacaaaa aagagccca aacattcaa caagaaacc 
        aaaccggaa cgagaaaaa atcgtcgaa gactacaca 
        aaaatcgtc ctcaaagac aacgaagtc actttcgac 
        tacaacgtc gacaaccac gtcacttat ttcgtcttg 
        agcggtttt tcggaccct ttgctgttc gaagagttg 
        tccaaaatt gatattgga gtgtcggca atcatcaac 
        gtttcgtta gaaaattca aacgaaaca ccttcggat 
        tttatcgtc aagttagac aatttgttt caaacacca 
        aaaacttct caatttttg gaaaacacc atacggatg 
        aagtacaga agcgacggt aaagaaccg acgaagaag 
        aaccttgaa gagctttta cgtgtggtc aagaaaatt 
        aattacttc aaacgccaa catctggcc tacatcagg 
        tcgctcaaa ctgtatgat tttggctca tttcgccat 
        gttgtgctc aacggtacg gaaaaatat agtcgaatt 
        atgaattat tacccgcct gatgtcgta aacattgcc 
        ctaattgtg gccgctttg gtgcaacaa atctgtgac 
        gaattgact ccaatttcg tcaatttcc gacttgctt 
        cagtcacca ttgggcgaa aaaacctat caagtgcac 
        gaagacgac gtccttaaa atgagcctc gagacttac 
        aaagaggtc ggaagggtg tgttacgac acaacactt 
        gactacctc aaactaatc caacccttg gctccaatt 
        gaatcaatg tccaaaggg accagactc caaaatcga 
        gcaatttca accgaagat gactgttat tgtgacaac 
        accaaagac ctgttcgat tatttccac ttggcaatg 
        ttcaaccaa atgacacga actaaattt gtgccaaaa 
        acttttgtt tcgacagtt ttggaaagc tacaacgaa 
        gcaaaacaa taccaaaat tcgctccct cgaggcgaa 
        ttggcgtca gaacccgac gaaaacgaa gtcatcaaa 
        cactaccac atgaaggag gagttatcg acttcggtc 
        cttcgccag ttggttttc aaagcaatg aacagttac 
        tcctgttgg aggcgagaa ttttgtgca gaaagcgag 
        tcaatcctt gttaaattc cacgacaat ttggacgaa 
        tttggtatt gaaacaaag atctggaac caggcgtta 
        acaacaccg atttgtttg cgcgatttt tgccgcttt 
        gtggcggaa gaaagggaa gaaattgat caagaggtc 
        gaagaaatt gaagaatgt gaaggttta agcaacttg 
        aacgtgcca gagttgtac caagagtac cattattta 
        ttgggtgcg tccagtgaa caaacgatg gaagaagcg 
        aaacgaatt ttcaacgag acaattcaa gaattggtt 
        gattatccc aatagtcac gctttgagg agttattgt 
        tctgaagat ggtttcgtc gcgtatgat ttggggccg 
        aaacgttgc caattaact ggtcatact acaattttt 
        cggagtgat gatggtgtt aaagttaca atagatacg 
        tcaaggttc atggttgag gacccaaaa tgtaccatc 
        acgctaaca gcagacgac aacagtttg atttttcat 
        gcggaacgc ccttcgaaa tttaacttt gttctaagg 
        gacggcact attgtcaat ttcctggaa tttggccaa 
        aattttgaa gatcaccag gtgaaagaa gaaattgac 
        gaaaaactg agtcatgat gaatcgatt ccaggcgaa 
        gagacacag tcaagttac aacgacctt tggttgcaa 
        attttacgt aaaagtaag attcaagaa acgattcca 
        aaatttttg gacaaaaga gtgcaacta gacgaaggg 
        aatttagat cgtttcttg gaacttttc aaagaggcg 
        gtttacacc caaagaccg atttacaaa atatcaaca 
        aaatcgtcc cttcgccac ttgaaacgt aagacttcg 
        ccggtcttt gtcccactt gttagtatc ttgagacgt 
        gtcttgttc aaaccagtt cgccggaag cgtaaaatt 
        ggttggagc gttcttaat atccctaat gaagtgaaa 
        aaggggccc ttgaagtat gattttcag ataaccctt 
        cctaatgga ttatttgtt agtaccctt ccgaagaac 
        cgtcaagtg actgaagtg aagcaaggg tacaacttt 
        aattgttac acagagaag taccgaatt ttttattcg 
        aatggagtg attgtgatc aaaaattgc gacggttcg 
        acgacaatt cttcgctca aacggcgat ataattgaa 
        ttaactgac gaaagcgaa tgttatggt aatgactgt 
        gctcgaaat atggctcaa ttttacaaa aacgaagac 
        gaagaaacg tgtccttat cgaacgact cgatgttcc 
        gaagctccg aaagttaaa gtgaggagt gatttcgtc 
        gatttgtac gaatttggt gacattcca tacgcgagg 
        aagagttta ttggacttt aacggaaaa aaagtttcg 
        gtttccgat ggtctagtg accgaaaca caactctat 
        tacgtcgtg caagaacac gattatcac gctgaggaa 
        gtgttttat gagagggag gacggcatg aagtgtgtg 
        atcgataaa aaggggaag cgaatcttc cagtttcct 
        gatggtact aaaataaca tcatggccc gaatttcaa 
        aacgatttg atccaagtc agcacaaac cccaaagtg 
        gaagggtgg gtttccgtt gtgacaaac tacaaattt 
        gaacatcca gactatatc gcaattctt tacgatgga 
        gttcaggga tcgactcag attttcctc aaagatggt 
        gttcttaaa tcgagtcaa gaaagcttg acaatgacc 
        acgaagaaa aatatagcg ttggaggtt tgctccgac 
        tgtataatt tttctcaaa acttgtgac acgtgtgat 
        ggtgattgt atctctaaa atacacctg gaccaaaat 
        tccaaattt ttggaagtg tatgacacc tacgacaag 
        tacctctgg tgtgattct tcaggaagg tgtgaaacc 
        aactctaat tatatcaaa ttaggggtt aataaaaaa 
        ggttgtgac catcgatgg aaaaacgag aaccaacaa 
        ctattcatt ttgaatggt cgtggtcca cctggaacc 
        cttctctgg aaagacagt tcgatagaa gcaaaaatc 
        ggcgaaagc aagttaatc aaaagccgc gtcgtaaat 
        tacatgaac aagttgacc aaaaaacaa actttggag 
        ttttacact gatgtgcac caggatttt ccaggaagg 
        ttcactatg tcgcaaatg tacaaccac gacatttgt 
        ttgtcgaaa ttcaaacat tcggacgag aaaccacta 
        ttttacgtt acgtatcgg gttttgata gagtttcaa 
        aacgtggca gaacacccg gaaatcatt gcagagttg 
        gatgagttt ttggaacgg aacagtaga ggcatttcg 
        attggtttg ttcgaagga attaaaacg attctaaat 
        tgtgttgtt gaggagttg aaacaagac aagtgtttg 
        tatcaggtg agcatgcaa gaggtggaa atgtccgac 
        cagactaca agtagtttg agcaaaagc ttagatttg 
        gatcggacg acggttcaa gatattgtg gcgcagtgg 
        aaaaaaaaa cccaaaggg accacactc caaaatcgg 
        tcaatttcg accgaagac gactgttat tgtcatgat 
        accaaagac ctgttcgat tatttccac ttggcaatg 
        ttcaaccaa atgacacga acgaaattc gtgccaaaa 
        actttcgtt tcgacagtt ttggaacgc tacgacgaa 
        gcgaaacag taccaaaat tcgctccct cgaggcgaa 
        tttgcgtca gaacccgac gaaaacaaa gtcatcaaa 
        cactaccac atgaaggag gagttatcg acttcggtc 
        cttcgccag ttggttttc aaagcaatg aacagttac 
        tcctgttgg aggcgcgaa ttttgtgaa gaaagcgag 
        tcaatcctt gttaaattc cacgacaat ttggacgac 
        tttggtttt gaaacaaag atctggaac caggcgtta 
        acaacaccg atttgtttg cgcgatttt tgccgcttt 
        gtggcggaa gaaagggaa gaaattgat caagaggac 
        gaagaaatt gaagaatgt gaaggttta agcaacttg 
        aacgtgcca gaattgtac caagagtac cattattta 
        ttgggtacg tctagtaga caaacgatg gaagaaacg 
        aaacgaatt ttcaacgag acaattcaa gaattggtt 
        gattatccc aatagtcac gctttgagg agttattgt 
        tctgaagat ggtttcgtc gcgtatgat ttggggccg 
        aaacgttgc caattaact ggtcatact acaattttt 
        cggagtgat gatggtgtt aaagttaca atagatacg 
        tcaaggttc atggttgag gacccaaaa tgtaccatc 
        acactaaca gcagacgac aacagtttg atttttcat 
        gcggaacgg ccttcgaaa tttaatttt gttctaagg 
        gacggcact attgtcaat tttctggaa tttggccaa 
        aattttgaa gatcaccag gtgaaagaa gaaattgac 
        gaaaaactg agtcatgat gaatcgatt tcaggcgaa 
        gaaacacag tcaagttac aacgacctt tggttgcaa 
        attttacgt aaaagtaaa attcaagaa acgtttcca 
        aaattttgg tacaaaagg gtgcaacta gacgaaggg 
        aatttagat cgtttcttg gaacttttc aaagaggcg 
        gtttacacc caaagaccg atttacaaa atatcaaca 
        aaatcgtcc cttcgccac ttgaaacgt aagacttcg 
        ccggtcttt gtcccactt gttagtatc ttgagacgt 
        gtcttgttt aaaccaatt cgccggaag cgtaaaatt 
        ggttggagc gtccttaat atccctaat gtaatgaaa 
        aaggggccc ttgaagtat gattttcag ataaccctt 
        cccaatgga ttatttgtt agtaccctt ccgaagaac 
        cgtcaagtg actgaagtg aagcaaggg tacaacttt 
        aattgttac acagagaag taccgaatt ttttattcg 
        aatggagtg attgtgatc aaaaattgc gacggttcg 
        acgacaatt cttcgctca aacggcgat ataatcgaa 
        ttaactgat gaaagtgaa tgttatggt aatgactgt 
        gcgcgaaat atggctcaa ttttacaaa aacgaagac 
        gaagaaacg tgtccttat cgaacgact cgttgttcc 
        gaagctccg aaagttaaa gtgaggagt gatttcgtc 
        gatttgtat gaatttggt gacattcca tacgcgagg 
        aagagttta ttggacttt aacggaaaa aaagtttcg 
        gtttccgat ggtctagtg accgaaaca caactctat 
        tacgtcgtg caagagcac gattatcac gctgaggaa 
        gtgttttat gagagggaa gacggcatg aagtgtgtt 
        attgataaa aaggggaag cgaatcttc cagtttcct 
        gatggtact aaaataaca tcatggccc gaatttcaa 
        aacgatttg atccaagtc agcacaaac cccaaggtg 
        gaagggtgg gtttccgtt gtgacaaat tacaaattt 
        gaacatcca gactatatc gcaattctt tacgatgga 
        gttcaggga tcgactcag attttcctc aaagatggt 
        gttcttaaa tcgagtcaa gaaagcttg acaatgacc 
        acgaagaaa aatatagcg ttggaggtt tgctccgac 
        tgtataatt ttcctcaaa acttgtgac acgtgtgat 
        ggtgattgt atttctaaa atacacctg gaccaaaac 
        tccaaactt ttggaagtg tatgacact tacgacaag 
        tacctctgg tgtgattct tcaggaagg tgtgaaacc 
        aactctaat tatatcaaa ttaggggtt aataaaaaa 
        ggttgtgac catcgatgg aaaaacgag aaccaacaa 
        ctattcatt ttgaatggt cgtggtcca cctggaacc 
        cttctctgg aaagacagt tcgatagaa gcaaaaatc 
        ggcgaaagc aagttaatc aaaagccgc gtcgtaaat 
        tacatgaac aagttgacc aaaaaacaa actttggag 
        ttttacact gatgtgcac caggatttt ccaggaagg 
        ttcactatg tcgcaaatg tacaaccac gacatttgc 
        ttgtcgaaa ttcaaacat tcggacgag aaaccacta 
        ttttacgtt atgtatcgg gttttgata gagtttcaa 
        aacgtggca gaacacccg gaaatcatt gcagagttg 
        gatgagttt ttggaacgg aacagtaga ggcatttcg 
        attggtttg ttcgaagga attaaaacg attctaaat 
        tgtgttgtt gaggagttg aaacaagac aagtgtttg 
        tatcaggtg agcatgcaa gaggtggaa atgtcggac 
        cagactaca agtagtttg agcaaaagc ttagatttg 
        gatcggacg acggttcaa gatattgtg gcgcagtgg 
        aaaaaaagg cccaagggg accacactc caaaatcgg 
        tcaatttcg accgaagac gactgttat tgtcataat 
        accaaagac ctgttcgat tatttccac ttggcaatg 
        ttcaaccaa atgacacga actaaattt gtgccaaaa 
        acttttgtt tcgacagtt ttggaacgc tacaacgaa 
        gcaagacaa taccaaaat tcgctccct cgagacgaa 
        tttgcgtca gaatccgac gaaaacaaa gtcatcaaa 
        cactaccac atgaaggag gagttatcg acttcggtt 
        cttcgccag ttggttttc aaagcaatg aacagttac 
        tcctgttgg aggcgagaa ttttgcgaa gaaagcgag 
        tcaatcctt gttaaattc cacgacaat ttggacgat 
        tttggtatt gaaacaaag atctggaac caggcatta 
        acaacaccg atttgtttg cgcgatttt tgccgcttt 
        gtagctgaa gaaagggga gaaattgat caagaggac 
        gaagaaatt gaagaatgt gaaggtttg agcaacttg 
        aacgcgcca gaattgtac caagagtac cattattta 
        ttgggtgcg tccagtgaa caaacgatg gaagaaacg 
        aaacaaatt ttcaacgag acaattcaa gaattggtt 
        gattatccc aatagtcac gctttgagg agttattgt 
        tctgaagat ggtttcgtc gcgtatgat ttagggccg 
        aaacgttgc caattaact ggtcatact acaattttt 
        cggagtgat gatggtgtt aaagttaca atgatacgt 
        caaggttca tggttgagg acccaaaat gtaccatca 
        ccctaacag cagaggaaa acaatttgg tttttcatg 
        cggaacggc cttcgaaat ttagctctg acctaaggg 
        acggcacta ttgtcaatt tcctggggt ttggctaaa 
        agttgtgac gagttgaaa tttttgata aggaataaa 
        aagtttccg ccgtatttc cacgcagac tattatcgt 
        catgtcttg ggggcaata aaaactaat gaatttg