Status
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Gene information
General information
TC001907 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450850.1: 4138..14823
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001907
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00310

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001907 RA
      MIHKGVTIC AFLATIGGI CRPVFAQNG FLDLNRKVR 
      SVSKRRFPR LENVYSHVV KPLEDFRKK YIGGVKNEK 
      KKFEKQTAK FCQCQERYL NLTTKKPNS LQEADASVD 
      MAERHFFTA SMEYVYLIQ EVHERKKFE FVETLLTFL 
      FGWLTFYHQ GHEVAKDFR PYTVDLQHK IQKTRANFD 
      DYSNKLRKR MADVQHSEK PVKKNKGCR EGYLFLLEK 
      KAFGTTWTK HFCTYDKET KEFTMLPYN QLTTKTISP 
      PEAMVLTSC VRRMSDSID KRFCFDIQF DSKPGVVLT 
      FQALSEQDR KAWLDIMDG KEPTYTTPG VNKAQNSEE 
      RLLDDLGIQ FVKKCIEVL ESRGLEEQG IYRVVGVTS 
      KVNKLLNMG LDRRKCDKL SLEDPQEWE TKTITSALK 
      SYLRNLPEP LMTYKYHNG FIAAVKNET RQMRIHDVH 
      TLIHRLPKT NFEVIKILI KHLTNVSAK SDKNLMTVS 
      NLGVCFGPT LLRPEEETV ASIMDLKFY NIVVEILIE 
      NYEKIFNSE PDKTLPSEN TSPRNAAPP SNNYKHISE 
      HPNFGAGDG TEVVYARTY NSNHSNHVS PNYRPQMTC 
      TVKAYYEEP LTANMCSSL YSVHEGTNG GYHPQMSRS 
      EHIGPNCQP ILHQKSMQR FIPTVSSQS ESNIPNLPS 
      HSPTYFRPF NRIISVPNS TSSSNESVS STSRDGNNY 
      VATPKKSER SSIYMAYPK GYRSPGDVR VRTLYACLG 
      EHDGELSFE PNQIITNVK SSQEPGWLE GTLNGKTGL 
      IPENYVEIL P
    CDS sequence
    • >TC001907 RA
      atgattcac aaaggcgtg acaatttgc gcctttttg 
      gccacaatc ggtggtatt tgccggcca gtttttgca 
      cagaatggg ttcttggac ttgaacagg aaagtcagg 
      agtgtatcg aagagaagg ttccccagg ctagagaac 
      gtctattcg cacgtggtg aagcccctc gaagacttt 
      cgtaagaaa tacatcggc ggagtgaaa aacgagaag 
      aaaaaattc gagaagcag accgctaaa ttctgtcag 
      tgccaagag agatattta aatctcacg acgaaaaaa 
      ccgaactcg cttcaagag gccgacgct tctgtagac 
      atggcagaa cgccacttc ttcacggcc agcatggaa 
      tacgtctat ctgatccaa gaagtgcat gagcggaaa 
      aaattcgag ttcgtagag acactttta acgtttctg 
      ttcggatgg ctcaccttc taccaccag gggcatgaa 
      gtagccaaa gactttcgg ccctacaca gtcgacttg 
      caacacaaa atacaaaaa acacgcgcc aattttgat 
      gactacagc aataaattg cggaaacga atggccgac 
      gttcaacac tccgaaaaa ccggtcaaa aagaataag 
      ggttgcagg gagggctac ctctttctt ttggagaaa 
      aaagctttt ggtaccaca tggaccaaa catttttgc 
      acatatgac aaagaaact aaagagttt acgatgtta 
      ccgtacaat cagctaaca accaaaacg atttcgcca 
      cctgaagcc atggtcttg acgtcgtgt gttaggcga 
      atgtctgat tccatcgat aagagattt tgtttcgat 
      attcagttt gattcaaaa cctggggtt gtgttaacc 
      ttccaggcg ctttcggag caagaccgg aaggcctgg 
      ttggatatt atggatggc aaagaaccg acttacaca 
      actccaggt gttaacaaa gcacaaaat tcggaagag 
      cgactcctc gacgattta ggcatccag tttgtgaaa 
      aaatgtatc gaagtgctg gaaagtcgc ggtttggag 
      gagcaaggc atctatcgg gtcgttgga gtcacttcg 
      aaagtgaac aaattgcta aacatgggc ttggatcgc 
      aggaaatgt gataaatta tctctggaa gatccccaa 
      gagtgggaa accaaaacc ataacgagc gctttgaaa 
      agttactta cggaacctc ccggaacca ctcatgact 
      tacaaatac cataacggg ttcatagcg gcagtcaaa 
      aatgagacc cgccaaatg aggatacac gacgtgcac 
      acgctaatc caccggtta ccaaaaacc aacttcgaa 
      gtgatcaaa atcctaatc aagcattta acgaatgtt 
      tccgccaaa tctgacaag aatttgatg actgtgagc 
      aacttgggt gtttgtttc gggccgact ttgctccgg 
      cccgaggaa gaaacggtt gcttcgatc atggatttg 
      aaattttat aacatcgtt gtggagatt ttgattgaa 
      aattatgag aaaatcttc aatagtgag cctgataaa 
      acattaccc agcgaaaac acttcgccg cgaaacgct 
      gcaccgcca agcaacaac tacaaacat atttcggag 
      catccgaat ttcggtgct ggcgatgga actgaagtt 
      gtttacgct cgaacgtac aactcgaat cattccaat 
      cacgtttcg cccaattac cggccgcaa atgacttgt 
      actgtgaag gcgtattac gaggaacct ctcactgcg 
      aatatgtgt tcaagtttg tacagtgtt cacgaaggg 
      acgaatggt ggctaccac ccacaaatg agtcgaagt 
      gaacacatt ggacctaat tgccaaccc attttacac 
      caaaaatca atgcaacgg ttcatacca acagtgtct 
      tctcagtct gaaagtaat attccaaat ttacctagt 
      cacagtcct acgtacttc agacctttc aaccgcatt 
      atttcagta cctaacagt accagcagt tcaaacgaa 
      tcagtctcg tcgacgtca cgcgacggc aataattac 
      gtcgcgacg cccaaaaaa agcgaaagg tcgagcatc 
      tacatggcg tatccgaaa ggctacagg tcgccaggt 
      gatgtgcga gtgcgaacg ttgtatgcg tgtttgggc 
      gaacatgac ggcgagttg tctttcgaa cccaatcag 
      attataacg aacgtgaaa agctcgcaa gagccgggg 
      tggttggag ggcactttg aacgggaaa actggtcta 
      ataccggaa aactatgtg gaaattttg ccctga
    mRNA sequence
    • >TC001907 RA
      taactcacg ttattagtt tacattatt aataactta 
      aaataattt agttgttaa cttatcgtg tggtttaag 
      gcgataacg tgatgattc acaaaggcg tgacaattt 
      gcgcctttt tggccacaa tcggtggta tttgccggc 
      cagtttttg cacagaatg ggttcttgg acttgaaca 
      ggaaagtca ggagtgtat cgaagagaa ggttcccca 
      ggctagaga acgtctatt cgcacgtgg tgaagcccc 
      tcgaagact ttcgtaaga aatacatcg gcggagtga 
      aaaacgaga agaaaaaat tcgagaagc agaccgcta 
      aattctgtc agtgccaag agagatatt taaatctca 
      cgacgaaaa aaccgaact cgcttcaag aggccgacg 
      cttctgtag acatggcag aacgccact tcttcacgg 
      ccagcatgg aatacgtct atctgatcc aagaagtgc 
      atgagcgga aaaaattcg agttcgtag agacacttt 
      taacgtttc tgttcggat ggctcacct tctaccacc 
      aggggcatg aagtagcca aagactttc ggccctaca 
      cagtcgact tgcaacaca aaatacaaa aaacacgcg 
      ccaattttg atgactaca gcaataaat tgcggaaac 
      gaatggccg acgttcaac actccgaaa aaccggtca 
      aaaagaata agggttgca gggagggct acctctttc 
      ttttggaga aaaaagctt ttggtacca catggacca 
      aacattttt gcacatatg acaaagaaa ctaaagagt 
      ttacgatgt taccgtaca atcagctaa caaccaaaa 
      cgatttcgc cacctgaag ccatggtct tgacgtcgt 
      gtgttaggc gaatgtctg attccatcg ataagagat 
      tttgtttcg atattcagt ttgattcaa aacctgggg 
      ttgtgttaa ccttccagg cgctttcgg agcaagacc 
      ggaaggcct ggttggata ttatggatg gcaaagaac 
      cgacttaca caactccag gtgttaaca aagcacaaa 
      attcggaag agcgactcc tcgacgatt taggcatcc 
      agtttgtga aaaaatgta tcgaagtgc tggaaagtc 
      gcggtttgg aggagcaag gcatctatc gggtcgttg 
      gagtcactt cgaaagtga acaaattgc taaacatgg 
      gcttggatc gcaggaaat gtgataaat tatctctgg 
      aagatcccc aagagtggg aaaccaaaa ccataacga 
      gcgctttga aaagttact tacggaacc tcccggaac 
      cactcatga cttacaaat accataacg ggttcatag 
      cggcagtca aaaatgaga cccgccaaa tgaggatac 
      acgacgtgc acacgctaa tccaccggt taccaaaaa 
      ccaacttcg aagtgatca aaatcctaa tcaagcatt 
      taacgaatg tttccgcca aatctgaca agaatttga 
      tgactgtga gcaacttgg gtgtttgtt tcgggccga 
      ctttgctcc ggcccgagg aagaaacgg ttgcttcga 
      tcatggatt tgaaatttt ataacatcg ttgtggaga 
      ttttgattg aaaattatg agaaaatct tcaatagtg 
      agcctgata aaacattac ccagcgaaa acacttcgc 
      cgcgaaacg ctgcaccgc caagcaaca actacaaac 
      atatttcgg agcatccga atttcggtg ctggcgatg 
      gaactgaag ttgtttacg ctcgaacgt acaactcga 
      atcattcca atcacgttt cgcccaatt accggccgc 
      aaatgactt gtactgtga aggcgtatt acgaggaac 
      ctctcactg cgaatatgt gttcaagtt tgtacagtg 
      ttcacgaag ggacgaatg gtggctacc acccacaaa 
      tgagtcgaa gtgaacaca ttggaccta attgccaac 
      ccattttac accaaaaat caatgcaac ggttcatac 
      caacagtgt cttctcagt ctgaaagta atattccaa 
      atttaccta gtcacagtc ctacgtact tcagacctt 
      tcaaccgca ttatttcag tacctaaca gtaccagca 
      gttcaaacg aatcagtct cgtcgacgt cacgcgacg 
      gcaataatt acgtcgcga cgcccaaaa aaagcgaaa 
      ggtcgagca tctacatgg cgtatccga aaggctaca 
      ggtcgccag gtgatgtgc gagtgcgaa cgttgtatg 
      cgtgtttgg gcgaacatg acggcgagt tgtctttcg 
      aacccaatc agattataa cgaacgtga aaagctcgc 
      aagagccgg ggtggttgg agggcactt tgaacggga 
      aaactggtc taataccgg aaaactatg tggaaattt 
      tgccctgac agaccggct ctctgtcga aaatttcca 
      cgagtctgc gtcatggcc tctgcaaag ctttacatt 
      tacacaatt ccgatcctg aactagtac aagaaaatc 
      cccgtttga gtttgcata gacagagtt tggacagcg 
      gcagagcgt tgctgagac aagcgagca ccgagcgaa 
      agagctcca cttaagcat atcacaact cagaaggtc 
      ccacctaac accatttac gtgtaaata agagcatat 
      taaccggtc accttttaa tttgttgtg attaacgcc 
      acctcgaag taataaaaa ctagcagcg tttttgaat 
      tatttttat aaagtatta tacacgttc tgtgatagt 
      aagacagac tggcacctc tagaagtac gttacatca 
      gccatacgt atcctgtat attgtaaaa taacttgat 
      tgtataatt tgtgggtgt gtattgtgt tggtacctt 
      taggattga gggaccaca accctgaaa caccaattg 
      tctcatatc aattgtgac tgcctataa ctgaagtaa 
      taatgtaaa aatccgatt caatagtta cctaactaa 
      atgtaatat caattgtgt ccttcaatg tacaagtct 
      taaatctca tttgtttta ttttattta atccacaaa 
      cacattatt atttgtgat acgacaaag tgtgtttac 
      cattatttt ttacaatat atattttat gtacaagta 
      ctcattttt tatgtatgg aatttatat ttgtatata 
      ttttattaa tctgttaat aattcttgt gtttagtta 
      ctgtaagaa ccattgtac ttgttgtat tatttccaa 
      gaaatgtcc gcaaacatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY