Status
Please wait ...
Gene information
General information
TC001885 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 233928..236401
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001885
    iBeetle screen iB_00308
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001885 RA
      MWDSISTDS CDVCQCPPR SKRGDDSCL DKENIQQAL 
      SPVKIEELS FYGNSSPPA IICSGTSPN HCNHNIITR 
      RPLEDHDPN SRDSGYVAN YNEGKFSTY ASPTRGSFE 
      LASIGSMED EFFEDFSDI EPLEDNLPQ DFGKLITGP 
      INFQNRKET PTSPRDLVI RPLFRRALS LQTERTPPS 
      RVRSCLFKT TESRPFKRP EPPQGFESP NSKKQKIED 
      EEAAVPVAR PVLQRCVSA TEESIKFAV QRSFVEPDL 
      IGDFSKTFC LPLTQGRHQ DLKCITADT LAKLIRGDF 
      SGNVASYKV IDCRYPYEY KGGHIDGAI NVYTKEQCM 
      ELLTNVASA SKEPRKRSN ILVFHCEFS SKRGPDLYR 
      YLREQDRQL NQDVYPSLH YPEVYLLEG GYKKFFEEY 
      SDLCVPIAY TEMLHPEYE NELRHFRLK SKTWNADTR 
      QRSGCRHNL KRLGL
    CDS sequence
    • >TC001885 RA
      atgtgggat tcgataagc acggattct tgtgatgtc 
      tgccagtgc cccccaaga tcgaaacga ggggacgat 
      tcttgtttg gataaagaa aatattcag caagcgtta 
      agtccggtc aaaatagaa gagttatcg ttttacgga 
      aattcgagt cctccggca ataatctgc tccgggact 
      tcaccgaat cactgcaac cacaatata ataactcgg 
      cgccccctt gaggaccac gaccccaat tcgcgcgat 
      tccggttac gttgctaat tacaatgag ggtaaattc 
      tcgacttat gcttccccg actcggggg tcgttcgaa 
      ctggcctcg atcgggtcc atggaggac gagtttttc 
      gaggatttt tccgatatt gagcccttg gaggacaac 
      ttgccgcag gatttcggg aaattgatc actggaccg 
      atcaatttc cagaaccgg aaagagaca ccgacttcg 
      ccgagggat ctagtaatt cggccctta tttcgaagg 
      gctttgtcc ttgcaaact gaaaggacg ccgccaagc 
      agggtcagg tcttgcctc ttcaaaaca acagaatct 
      cgcccgttt aagagacca gaaccgccg caaggattc 
      gaaagccca aattcgaaa aaacagaaa attgaggat 
      gaggaagcc gctgttcca gtcgcaagg cctgtgctt 
      cagagatgc gtgtctgcg acggaggag agtataaag 
      tttgcggtc cagaggtca ttcgtggag ccagacctc 
      atcggtgac ttcagcaag acgttttgt ttgccctta 
      acgcaaggc cgtcatcag gacttgaag tgcatcact 
      gctgacact ttggccaaa ctaatccgt ggtgacttt 
      agcggaaac gtcgcctct tacaaagtt atcgattgc 
      cggtaccct tacgagtac aagggtggc catattgac 
      ggcgccatt aacgtctac accaaggag cagtgcatg 
      gaactatta actaacgtc gcctctgcg agcaaggag 
      ccgcgcaaa aggagcaat atcctggtt ttccattgc 
      gagttttcg agcaagcgg ggtccagat ttatatcgg 
      tatttgcgt gagcaggac cgccagctc aaccaggac 
      gtctaccct tcgttgcac taccccgaa gtctaccta 
      cttgagggt ggctataag aagttcttc gaggaatat 
      tccgacctc tgcgttcct atagcctac acggagatg 
      ctgcacccg gaatatgag aatgagttg cgacatttc 
      cggctcaaa tccaaaacc tggaatgct gatacgcgt 
      caacggtcc gggtgccgt cacaatttg aaaagacta 
      ggactgtaa
    mRNA sequence
    • >TC001885 RA
      aggataaag actgaggta ggctacctt cacttccaa 
      gtcgcgttt gtgtggtgt gcgaggacg tatcgtcct 
      ggtttgagt gcttgttgc ttgtgttag attttaaga 
      cgttttaag gatatttca ccgctttct tccgacaat 
      gtgggattc gataagcac ggattcttg tgatgtctg 
      ccagtgccc cccaagatc gaaacgagg ggacgattc 
      ttgtttgga taaagaaaa tattcagca agcgttaag 
      tccggtcaa aatagaaga gttatcgtt ttacggaaa 
      ttcgagtcc tccggcaat aatctgctc cgggacttc 
      accgaatca ctgcaacca caatataat aactcggcg 
      cccccttga ggaccacga ccccaattc gcgcgattc 
      cggttacgt tgctaatta caatgaggg taaattctc 
      gacttatgc ttccccgac tcgggggtc gttcgaact 
      ggcctcgat cgggtccat ggaggacga gtttttcga 
      ggatttttc cgatattga gcccttgga ggacaactt 
      gccgcagga tttcgggaa attgatcac tggaccgat 
      caatttcca gaaccggaa agagacacc gacttcgcc 
      gagggatct agtaattcg gcccttatt tcgaagggc 
      tttgtcctt gcaaactga aaggacgcc gccaagcag 
      ggtcaggtc ttgcctctt caaaacaac agaatctcg 
      cccgtttaa gagaccaga accgccgca aggattcga 
      aagcccaaa ttcgaaaaa acagaaaat tgaggatga 
      ggaagccgc tgttccagt cgcaaggcc tgtgcttca 
      gagatgcgt gtctgcgac ggaggagag tataaagtt 
      tgcggtcca gaggtcatt cgtggagcc agacctcat 
      cggtgactt cagcaagac gttttgttt gcccttaac 
      gcaaggccg tcatcagga cttgaagtg catcactgc 
      tgacacttt ggccaaact aatccgtgg tgactttag 
      cggaaacgt cgcctctta caaagttat cgattgccg 
      gtaccctta cgagtacaa gggtggcca tattgacgg 
      cgccattaa cgtctacac caaggagca gtgcatgga 
      actattaac taacgtcgc ctctgcgag caaggagcc 
      gcgcaaaag gagcaatat cctggtttt ccattgcga 
      gttttcgag caagcgggg tccagattt atatcggta 
      tttgcgtga gcaggaccg ccagctcaa ccaggacgt 
      ctacccttc gttgcacta ccccgaagt ctacctact 
      tgagggtgg ctataagaa gttcttcga ggaatattc 
      cgacctctg cgttcctat agcctacac ggagatgct 
      gcacccgga atatgagaa tgagttgcg acatttccg 
      gctcaaatc caaaacctg gaatgctga tacgcgtca 
      acggtccgg gtgccgtca caatttgaa aagactagg 
      actgtaaat atcgaatcc cagtgccaa aatttgtga 
      gatagtaca aaatacaag tgcgcggac ttgtttgcc 
      aaatgatat gtacaaagt aatgtgcta tattagcat 
      gtatcctga agacttagt cttcttagt gtatattac 
      aactgtata taagagagt acctaaata gcttgctcc 
      agagtagtt gagatgaga tctttcctt ttgcaatgc 
      taaaccacc aaagttttc tttttgtta tttagacag 
      tcgacggat catcctggt cccccttta atttatttt 
      ttatgtgca atttgaaca gcaataacg atgtgatgt 
      tgcatttct ttaccagta cattatgct tccttgctg 
      ttgcgctgg tgtttgttt gcagatttt ttatgttca 
      ttttattag tttagtata gtctaagtg tcagtattg 
      tttttgtat ccttcagca tattcaaat gtgttcgaa 
      tgcaacggc tggggattt tatactact tgaatgtga 
      tttgttcac tatttggac ggactgatg cttacccat 
      ccgttgcat catagattt ttaaagtga tacttaata 
      ttatagatc tgtacaata ttttaatat ttttataac 
      ttatgataa tacattttt gttccacat