Status
Please wait ...
Gene information
General information
TC001775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7722261..7732726
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001775
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    prothoracic odoriferous glands potentially not present - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    abdominal odoriferous glands not present - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection



    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 10 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001775 RA
      MFIPDTLRS CMVEPDVSS YLQTPSSGQ DEFHPFIEA 
      LLPFVKSFS YTWFNLQAA KRKYYKKHE KRMSLEEER 
      QCKEALQNE KAEVKQKWA SRLLGKLRK DITQECRED 
      FVLSITGKR PAVCVLSNP DQKGKMRRI DCLRQADKV 
      WRLDLVMVI LFKAIPLES TDGERLEKN PDCSQPGLC 
      VNPYHINVS VRELDLYLA NFINSHEIL SGVCYNNNN 
      KKEDDRKNK VTGYTHNPY NGVICNDII LATGVFSSK 
      ELWKLSKSS ILHELSDPQ PTMNSVKLE HPAYYCNNY 
      SEQGPVVSD RTSSVDAMV VSAAYSIPQ NTTLVSSSS 
      QPNPSTIFY QHSPEPHQH QHQNQVKYP ENGHDTLSD 
      FVTFVCQET DSSNQNAQM QTNMSRSPK TQYFPSTML 
      PPPPLPPMA RPVAIIRST GDVTLSGTN SPPASITPP 
      CSEASPADD VPEISSSPP LSPGSHERR KSPSQRNSV 
      PTSSPYSPT RDYSFNHFH GQPGHLFSY PSMSGMSGV 
      ISPTNLSMY SASVSTSRG TRSSSIPRW SAPLFTLDQ 
      EDFNMIAHQ TNPESNAII LMDDGTASS DHGQCCSSA 
      NLMCDTDRF FQAGDALES AGQRDAELP APKSP
    CDS sequence
    • >TC001775 RA
      atgttcatc cccgacacg ttgcgcagc tgcatggtg 
      gagcccgat gtgtcctcg tatctccag acgccgtcc 
      tcgggacag gacgagttc cacccattc atcgaggca 
      ctcctgcct tttgtcaag tccttctca tacacgtgg 
      tttaatctc caagccgcc aaaaggaag tactacaaa 
      aaacacgag aaacggatg tctttggag gaggagcgg 
      cagtgcaaa gaagcttta cagaacgag aaagccgaa 
      gtcaagcag aaatgggcc tcccgtctg ttgggcaag 
      ctccggaag gacattacg caggaatgt cgcgaggac 
      ttcgtgctg agcatcacg gggaagcga cccgcagtc 
      tgcgtgctc tcaaatccc gatcagaaa ggcaaaatg 
      cgccgaata gactgtctc cgccaagca gataaagtt 
      tggcgcttg gatctcgtt atggtgata ctttttaaa 
      gcaatacct ctcgaaagt acggatggc gaaaggctc 
      gagaaaaat ccggactgt tctcaacct ggactttgt 
      gtaaatcca tatcatatt aatgtgtca gtgcgagaa 
      ctggatctt tatctagcc aacttcatc aacagccac 
      gaaatacta agcggagtc tgttacaat aacaacaac 
      aagaaagag gacgacagg aaaaacaaa gttacaggt 
      tacacccac aatccgtac aacggtgta atatgtaat 
      gacattata ttagctaca ggagtattt tcatcaaag 
      gaattatgg aagttgtcc aaatcttct atcttgcac 
      gaactttcg gatccgcaa ccaacgatg aacagtgtc 
      aaattagaa cacccggcc tattattgc aacaattat 
      tcagagcag gggcctgtt gtcagtgac cggacgtct 
      tcagtcgac gccatggtc gtctcagcg gcctactct 
      atacctcaa aacaccact ctagtgtct tccagttct 
      caacctaat ccaagtaca atattctat cagcacagt 
      ccggagcca caccaacac cagcaccaa aaccaggtg 
      aaatatcca gaaaacgga cacgacacc ctctccgac 
      tttgtcact ttcgtgtgt caagagacg gacagcagc 
      aaccaaaac gcacaaatg caaacgaac atgtctcga 
      agtccgaaa acgcagtat tttccgtcg acgatgttg 
      ccgccgccg cccctgccc ccgatggcc cgtccggtg 
      gcgataatc cgttcaact ggcgacgtg acgctaagc 
      gggacaaac tcgccccct gcgagcata acccctcca 
      tgcagcgag gcgtctccc gccgacgac gtccccgag 
      atatcgtcg tcgccgccg ctgagtcca ggcagccac 
      gagcggcgc aaaagcccc tcgcagagg aactccgtg 
      ccgaccagc agtccgtac tcgccgacg cgggactac 
      tccttcaac cactttcac gggcagccg ggacatctc 
      ttcagctac cccagtatg agcgggatg tctggagtt 
      atcagccct acgaacctc agcatgtac tcggccagc 
      gtgagtacg tccagaggt acgcgttct agcagcatc 
      ccgcgctgg agcgccccg ctgttcacc ctggaccag 
      gaggacttc aacatgatt gcgcaccag acgaacccg 
      gagtccaac gccatcatc ctgatggac gacggcacg 
      gccagttcc gaccacgga cagtgctgc agcagtgcg 
      aatcttatg tgcgatacg gaccgattc tttcaagcg 
      ggcgatgcc ttagagagc gcgggccag agggacgca 
      gaactgccc gcaccgaag tccccgtga
    mRNA sequence
    • >TC001775 RA
      gatggcagc accagtcga tcgcgagtg cctcttcga 
      gcagtagtg cgaagcaga aagcagcag atgaggaaa 
      aggatgaag cggcagcgc ccggacgca agtcgacgt 
      catcggcgt cgtagcaat atataaata tatacggcg 
      ctggcgacg tcccgcgtc ttttattaa tagagcgcg 
      agaggcgag cgcggggtc ctgcagcgg acatttgct 
      aggtgttgg gtacgtgca acgtgcgga cggctgatg 
      tagacgttc gcggagctc gaggtggca tgtgtggga 
      ctagtgatg agtgagttg gaatcgtcg ccaagcaca 
      agaagaggc cgaggacga gcaagagca agagcaaga 
      gcagaggct gcggacggt gcccgcgtc cgcgtccgc 
      agtgcgcat taggcaatc aatgaggag gaggctgct 
      ggtagcact agttctctt ctggtgatg ggacccgga 
      ccggtgcgc gccgcccgc cggtgacgg tgacgtgac 
      ctgaggaat gttcatccc cgacacgtt gcgcagctg 
      catggtgga gcccgatgt gtcctcgta tctccagac 
      gccgtcctc gggacagga cgagttcca cccattcat 
      cgaggcact cctgccttt tgtcaagtc cttctcata 
      cacgtggtt taatctcca agccgccaa aaggaagta 
      ctacaaaaa acacgagaa acggatgtc tttggagga 
      ggagcggca gtgcaaaga agctttaca gaacgagaa 
      agccgaagt caagcagaa atgggcctc ccgtctgtt 
      gggcaagct ccggaagga cattacgca ggaatgtcg 
      cgaggactt cgtgctgag catcacggg gaagcgacc 
      cgcagtctg cgtgctctc aaatcccga tcagaaagg 
      caaaatgcg ccgaataga ctgtctccg ccaagcaga 
      taaagtttg gcgcttgga tctcgttat ggtgatact 
      ttttaaagc aatacctct cgaaagtac ggatggcga 
      aaggctcga gaaaaatcc ggactgttc tcaacctgg 
      actttgtgt aaatccata tcatattaa tgtgtcagt 
      gcgagaact ggatcttta tctagccaa cttcatcaa 
      cagccacga aatactaag cggagtctg ttacaataa 
      caacaacaa gaaagagga cgacaggaa aaacaaagt 
      tacaggtta cacccacaa tccgtacaa cggtgtaat 
      atgtaatga cattatatt agctacagg agtattttc 
      atcaaagga attatggaa gttgtccaa atcttctat 
      cttgcacga actttcgga tccgcaacc aacgatgaa 
      cagtgtcaa attagaaca cccggccta ttattgcaa 
      caattattc agagcaggg gcctgttgt cagtgaccg 
      gacgtcttc agtcgacgc catggtcgt ctcagcggc 
      ctactctat acctcaaaa caccactct agtgtcttc 
      cagttctca acctaatcc aagtacaat attctatca 
      gcacagtcc ggagccaca ccaacacca gcaccaaaa 
      ccaggtgaa atatccaga aaacggaca cgacaccct 
      ctccgactt tgtcacttt cgtgtgtca agagacgga 
      cagcagcaa ccaaaacgc acaaatgca aacgaacat 
      gtctcgaag tccgaaaac gcagtattt tccgtcgac 
      gatgttgcc gccgccgcc cctgccccc gatggcccg 
      tccggtggc gataatccg ttcaactgg cgacgtgac 
      gctaagcgg gacaaactc gccccctgc gagcataac 
      ccctccatg cagcgaggc gtctcccgc cgacgacgt 
      ccccgagat atcgtcgtc gccgccgct gagtccagg 
      cagccacga gcggcgcaa aagcccctc gcagaggaa 
      ctccgtgcc gaccagcag tccgtactc gccgacgcg 
      ggactactc cttcaacca ctttcacgg gcagccggg 
      acatctctt cagctaccc cagtatgag cgggatgtc 
      tggagttat cagccctac gaacctcag catgtactc 
      ggccagcgt gagtacgtc cagaggtac gcgttctag 
      cagcatccc gcgctggag cgccccgct gttcaccct 
      ggaccagga ggacttcaa catgattgc gcaccagac 
      gaacccgga gtccaacgc catcatcct gatggacga 
      cggcacggc cagttccga ccacggaca gtgctgcag 
      cagtgcgaa tcttatgtg cgatacgga ccgattctt 
      tcaagcggg cgatgcctt agagagcgc gggccagag 
      ggacgcaga actgcccgc accgaagtc cccgtgagc 
      atcgacgac gacgacgac gacgacaaa ctgccttat 
      cgctttctt gccaattta tgtgtgatt attatgttg 
      gattttgat catttcacg cgactcggt gcgctatag 
      gctgctcaa ctagcgtag attaggtta ctgtataca 
      tacttatag tatagaccg gtgccaaga caggctgtg 
      gcgatccag aaggacccg gcacgtcgg aaagacact 
      attcgacct gctcggccc aacggcctg aacacgttg 
      gtgacgtta gtttcaatt ccgttccta tgcggggac 
      acaccccac catcgcccc ttccacacc actcgccaa 
      aaccggtta aaccggctt ctgcaaatt caccacctt 
      tcctactcg taacttggg aacttaaca aagatatac 
      ccggttagg aattttttg atataaatc tgagctttg 
      ttagcggtt ctaaatgag ttacgaatt ttgggccac 
      cacaaacga taatttatc gttgtcaat taaatgttc 
      agaattgtt accgtaatc gcgaacaaa ttattctac 
      atgtagtat caaatccca caatttttc attttaaaa 
      agcaagtgg aaatcttct tagttgccg agttcccaa 
      tattgtcac ttaaaccgg tttaaacgg ctgcaacaa 
      aacttgaaa aaaacaaaa tacgtagtt acacagcaa 
      gtcgctcgt tgcgaaata ttgcaacga aattgggtg 
      agtaataaa tatattata agatataac gtatttcta 
      tgtgccaca gcgtgcgac atctctatt gcccttcca 
      atgaatcgt ggtttctaa ttagaattt tataatgtc 
      ttgaaattt tacgaaata ttctgaggt aattgggaa 
      aatatgagg gaattgcac gaaatctcg aaaaaatct 
      ggtttacaa catacatag tacctatgg aaataaaat 
      tattgaaat tttacacac ttgactgtg caatgataa 
      caatcacta tcaattttt atattttat tttgtaatt 
      aaataaagt cgtagcagt ctcgtgagt tttcgggga 
      ctaactgtt aatttaact aagttttta cactaagtg 
      aatttattt taaattttt tacttgagc ggttacata 
      atcactata caacaaatt ttaactttt ttttgttta 
      tgggatttt ttacaatct tcaatttaa aatcattag 
      ttaagtgga taaaataaa ttttatact actct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY