| General information | |||
| TC001771 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7761526..7765746 | |
| Sequence information | |||
| Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001771 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00303 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks - cuticles: eggs lost,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
remarks: animals lost,
- >TC001771 RA
MALARKTQF VINCSQSDY YIHCIINSG VILDEDMEL ILTYFASDD RYSQEIKLS VLQKIIGCV SLAGTIFLV ERKYPLYLL PISATPMFL MMLKTMRRS IQQKTQQRN FEVFVETTG KIAKLNRVI CEYFKHRNV PSTETSDIL NPYTTAMKK LLEVVFMEE TTLLYKLRL CVREFVGYV PFLKKDYTH VDYIEIHTL TSLKIRNIN DCVIQLKQL CDIYVLLAS NCLTSLSVA LSPRVWSIY RVDLEYILE VLIPRMRKL SEQCYSAIS KKFDDLKFY NLNRGQVKK RMMKIKRHN GQTKLYLGT CDIMVYISD IIEKSEIVY KKLQEHNLQ TMHEREVVG KCIAELRTH VFEYYEALD VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG LVSISENSA EICNFLFSI FQKDEDPEV TTEEVPPRL FRVPEHLPP LPPLAPVPQ FRPVPLALP NDQPARPLV PPPPPPPLP PIDLGEFEE PPPQEGDYT VNHFLDSLK AAAQSRSSS EEVFQD
- >TC001771 RB
MAFNQHNLQ TMHEREVVG KCIAELRTH VFEYYEALD VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG LVSISENSA EICNFLFSI FQKDEDPEV TTEEVPPRL FRVPEHLPP LPPLAPVPQ FRPVPLALP NDQPARPLV PPPPPPPLP PIDLGEFEE PPPQEGDYT VNHFLDSLK AAAQSRSSS EEVFQD
- >TC001771 RC
MAFNQHNLQ TMHEREVVG KCIAELRTH VFEYYEALD VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG LKDEDPEVT TEEVPPRLF RVPEHLPPL PPLAPVPQF RPVPLALPN DQPARPLVP PPPPPPLPP IDLGEFEEP PPQEGDYTV NHFLDSLKA AAQSRSSSE EVFQD
- >TC001771 RD
MALARKTQF VINCSQSDY YIHCIINSG VILDEDMEL ILTYFASDD RYSQEIKLS VLQKIIGCV SLAGTIFLV ERKYPLYLL PISATPMFL MMLKTMRRS IQQKTQQRN FEVFVETTG KIAKLNRVI CEYFKHRNV PSTETSDIL NPYTTAMKK LLEVVFMEE TTLLYKLRL CVREFVGYV PFLKKDYTH VDYIEIHTL TSLKIRNIN DCVIQLKQL CDIYVLLAS NCLTSLSVA LSPRVWSIY RVDLEYILE VLIPRMRKL SEQCYSAIS KKFDDLKFY NLNRGQVKK RMMKIKRHN GQTKLYLGT CDIMVYISD IIEKSEIVY KKLQEVSLV FILIKRRGR RYTKII
- >TC001771 RA
atggcacta gcacggaaa actcaattt gttatcaat tgctcgcaa tccgattac tacattcat tgcattatt aattctgga gtcatctta gacgaagac atggagtta attttgacg tatttcgca agtgatgat cgttacagt caggagatt aagttatcg gttttgcaa aaaataatt ggttgtgtc tctttggct ggaacgatc tttttggtg gaaagaaaa taccccctt tatcttttg ccgatttcg gccacacca atgtttttg atgatgctt aaaacaatg cgccgctcc attcaacaa aaaacacaa caacgtaac ttcgaggtt tttgttgaa acgactggt aagatcgcg aaactaaat cgcgtgatt tgtgaatat ttcaagcac agaaacgtg ccaagtact gaaacttcc gatattctc aatccgtac acgactgca atgaagaag ttgctcgaa gtagttttc atggaggaa accactttg ttatacaaa ctacgactt tgcgtacgc gaatttgtc ggttacgtg cctttcctt aaaaaggac tatacccat gtggactat attgaaata cataccttg accagccta aagattcgc aatattaat gactgtgtt attcaactg aaacaactc tgtgatatc tatgtatta ctagcttcc aattgccta acttcgttg tcggtggct ttgagcccg cgagtttgg tccatatat agagttgat ctggagtat atcttggag gtactcatt ccgagaatg agaaaactc tctgaacaa tgttacagc gctataagc aaaaaattc gatgattta aaattctat aacttgaac cgaggccag gtcaaaaag cgcatgatg aagatcaaa cgccataat gggcagaca aaactgtat ttggggact tgcgatata atggtatac atttcagac atcattgag aagtctgaa atagtgtac aaaaaattg caggagcac aatctccaa accatgcac gagcgcgaa gttgttgga aagtgcatt gcggagctc cgcactcac gtttttgaa tactacgag gctttggat gttttgcat aaacattac gagataaat ctcaacaaa ccaaaacgc gatccgcaa tcaaagctc gttgagata ccagaggat ggccctatt ccctcaacc agtcgcggg aacgctgct gatgcgagc ttgaatcct gttgaaccg gacaaagac gaggaattt gaggcttac gttggggcc aatgataat aattccgat agtagcacc agccacagt gcttacgat gagcaggaa aaccgcaga tacgcctct ctgctgagc caagaactg aagtccaaa ttgataact cacgccact ttcatggcc gcaagacaa cgcagagga cttgtcagt atttcagaa aatagtgcc gagatctgc aactttctc ttttcaatt tttcagaag gacgaagac cctgaagtg actactgaa gaggtgcct ccacgactt ttcagggtg cccgaacat ttgccaccg ctgcctcct ctcgctcct gtgccacaa tttcgtcct gttccactt gcattaccc aacgatcaa cctgccagg cctctcgtt cctcccccg cccccaccg ccgctaccg ccaattgat ttgggcgaa tttgaggag ccgccccca caagagggc gattacact gtgaaccac ttcctcgac tctctcaaa gccgcagct cagagtcgc tccagcagt gaggaggtc ttccaagat taa
- >TC001771 RB
atggctttc aatcagcac aatctccaa accatgcac gagcgcgaa gttgttgga aagtgcatt gcggagctc cgcactcac gtttttgaa tactacgag gctttggat gttttgcat aaacattac gagataaat ctcaacaaa ccaaaacgc gatccgcaa tcaaagctc gttgagata ccagaggat ggccctatt ccctcaacc agtcgcggg aacgctgct gatgcgagc ttgaatcct gttgaaccg gacaaagac gaggaattt gaggcttac gttggggcc aatgataat aattccgat agtagcacc agccacagt gcttacgat gagcaggaa aaccgcaga tacgcctct ctgctgagc caagaactg aagtccaaa ttgataact cacgccact ttcatggcc gcaagacaa cgcagagga cttgtcagt atttcagaa aatagtgcc gagatctgc aactttctc ttttcaatt tttcagaag gacgaagac cctgaagtg actactgaa gaggtgcct ccacgactt ttcagggtg cccgaacat ttgccaccg ctgcctcct ctcgctcct gtgccacaa tttcgtcct gttccactt gcattaccc aacgatcaa cctgccagg cctctcgtt cctcccccg cccccaccg ccgctaccg ccaattgat ttgggcgaa tttgaggag ccgccccca caagagggc gattacact gtgaaccac ttcctcgac tctctcaaa gccgcagct cagagtcgc tccagcagt gaggaggtc ttccaagat taa
- >TC001771 RC
atggctttc aatcagcac aatctccaa accatgcac gagcgcgaa gttgttgga aagtgcatt gcggagctc cgcactcac gtttttgaa tactacgag gctttggat gttttgcat aaacattac gagataaat ctcaacaaa ccaaaacgc gatccgcaa tcaaagctc gttgagata ccagaggat ggccctatt ccctcaacc agtcgcggg aacgctgct gatgcgagc ttgaatcct gttgaaccg gacaaagac gaggaattt gaggcttac gttggggcc aatgataat aattccgat agtagcacc agccacagt gcttacgat gagcaggaa aaccgcaga tacgcctct ctgctgagc caagaactg aagtccaaa ttgataact cacgccact ttcatggcc gcaagacaa cgcagagga cttaaggac gaagaccct gaagtgact actgaagag gtgcctcca cgacttttc agggtgccc gaacatttg ccaccgctg cctcctctc gctcctgtg ccacaattt cgtcctgtt ccacttgca ttacccaac gatcaacct gccaggcct ctcgttcct cccccgccc ccaccgccg ctaccgcca attgatttg ggcgaattt gaggagccg cccccacaa gagggcgat tacactgtg aaccacttc ctcgactct ctcaaagcc gcagctcag agtcgctcc agcagtgag gaggtcttc caagattaa
- >TC001771 RD
atggcacta gcacggaaa actcaattt gttatcaat tgctcgcaa tccgattac tacattcat tgcattatt aattctgga gtcatctta gacgaagac atggagtta attttgacg tatttcgca agtgatgat cgttacagt caggagatt aagttatcg gttttgcaa aaaataatt ggttgtgtc tctttggct ggaacgatc tttttggtg gaaagaaaa taccccctt tatcttttg ccgatttcg gccacacca atgtttttg atgatgctt aaaacaatg cgccgctcc attcaacaa aaaacacaa caacgtaac ttcgaggtt tttgttgaa acgactggt aagatcgcg aaactaaat cgcgtgatt tgtgaatat ttcaagcac agaaacgtg ccaagtact gaaacttcc gatattctc aatccgtac acgactgca atgaagaag ttgctcgaa gtagttttc atggaggaa accactttg ttatacaaa ctacgactt tgcgtacgc gaatttgtc ggttacgtg cctttcctt aaaaaggac tatacccat gtggactat attgaaata cataccttg accagccta aagattcgc aatattaat gactgtgtt attcaactg aaacaactc tgtgatatc tatgtatta ctagcttcc aattgccta acttcgttg tcggtggct ttgagcccg cgagtttgg tccatatat agagttgat ctggagtat atcttggag gtactcatt ccgagaatg agaaaactc tctgaacaa tgttacagc gctataagc aaaaaattc gatgattta aaattctat aacttgaac cgaggccag gtcaaaaag cgcatgatg aagatcaaa cgccataat gggcagaca aaactgtat ttggggact tgcgatata atggtatac atttcagac atcattgag aagtctgaa atagtgtac aaaaaattg caggaggtg agtcttgtt tttatattg attaaacga cgagggagg aggtataca aagattata taa
- >TC001771 RA
tttcgcaaa agtcaaatt ttcatcaaa gtttttgtc tacgttatt tgtgagtat tttcatagt taaagttgt ttattctga tattattac acaagaatc gacgctggg ttgggccta gtacaccag agcgacgaa atttgctac ttccaggaa gtccaggga agtgcaaaa acatcgaaa atggcacta gcacggaaa actcaattt gttatcaat tgctcgcaa tccgattac tacattcat tgcattatt aattctgga gtcatctta gacgaagac atggagtta attttgacg tatttcgca agtgatgat cgttacagt caggagatt aagttatcg gttttgcaa aaaataatt ggttgtgtc tctttggct ggaacgatc tttttggtg gaaagaaaa taccccctt tatcttttg ccgatttcg gccacacca atgtttttg atgatgctt aaaacaatg cgccgctcc attcaacaa aaaacacaa caacgtaac ttcgaggtt tttgttgaa acgactggt aagatcgcg aaactaaat cgcgtgatt tgtgaatat ttcaagcac agaaacgtg ccaagtact gaaacttcc gatattctc aatccgtac acgactgca atgaagaag ttgctcgaa gtagttttc atggaggaa accactttg ttatacaaa ctacgactt tgcgtacgc gaatttgtc ggttacgtg cctttcctt aaaaaggac tatacccat gtggactat attgaaata cataccttg accagccta aagattcgc aatattaat gactgtgtt attcaactg aaacaactc tgtgatatc tatgtatta ctagcttcc aattgccta acttcgttg tcggtggct ttgagcccg cgagtttgg tccatatat agagttgat ctggagtat atcttggag gtactcatt ccgagaatg agaaaactc tctgaacaa tgttacagc gctataagc aaaaaattc gatgattta aaattctat aacttgaac cgaggccag gtcaaaaag cgcatgatg aagatcaaa cgccataat gggcagaca aaactgtat ttggggact tgcgatata atggtatac atttcagac atcattgag aagtctgaa atagtgtac aaaaaattg caggagcac aatctccaa accatgcac gagcgcgaa gttgttgga aagtgcatt gcggagctc cgcactcac gtttttgaa tactacgag gctttggat gttttgcat aaacattac gagataaat ctcaacaaa ccaaaacgc gatccgcaa tcaaagctc gttgagata ccagaggat ggccctatt ccctcaacc agtcgcggg aacgctgct gatgcgagc ttgaatcct gttgaaccg gacaaagac gaggaattt gaggcttac gttggggcc aatgataat aattccgat agtagcacc agccacagt gcttacgat gagcaggaa aaccgcaga tacgcctct ctgctgagc caagaactg aagtccaaa ttgataact cacgccact ttcatggcc gcaagacaa cgcagagga cttgtcagt atttcagaa aatagtgcc gagatctgc aactttctc ttttcaatt tttcagaag gacgaagac cctgaagtg actactgaa gaggtgcct ccacgactt ttcagggtg cccgaacat ttgccaccg ctgcctcct ctcgctcct gtgccacaa tttcgtcct gttccactt gcattaccc aacgatcaa cctgccagg cctctcgtt cctcccccg cccccaccg ccgctaccg ccaattgat ttgggcgaa tttgaggag ccgccccca caagagggc gattacact gtgaaccac ttcctcgac tctctcaaa gccgcagct cagagtcgc tccagcagt gaggaggtc ttccaagat taaattttg ttcgcatgt ttttaaatt ttgttaacg tttgatgtg ttcagttta gtttttgtt ctttgttca ttcttttgt acttaattc gtttgcgtt caaataaat gttaaaaaa atac
- >TC001771 RB
gacctctta acagattag ggagggcaa ttttttatt taattgata aaaacaatt acaaatggc tttcaatca gcacaatct ccaaaccat gcacgagcg cgaagttgt tggaaagtg cattgcgga gctccgcac tcacgtttt tgaatacta cgaggcttt ggatgtttt gcataaaca ttacgagat aaatctcaa caaaccaaa acgcgatcc gcaatcaaa gctcgttga gataccaga ggatggccc tattccctc aaccagtcg cgggaacgc tgctgatgc gagcttgaa tcctgttga accggacaa agacgagga atttgaggc ttacgttgg ggccaatga taataattc cgatagtag caccagcca cagtgctta cgatgagca ggaaaaccg cagatacgc ctctctgct gagccaaga actgaagtc caaattgat aactcacgc cactttcat ggccgcaag acaacgcag aggacttgt cagtatttc agaaaatag tgccgagat ctgcaactt tctcttttc aatttttca gaaggacga agaccctga agtgactac tgaagaggt gcctccacg acttttcag ggtgcccga acatttgcc accgctgcc tcctctcgc tcctgtgcc acaatttcg tcctgttcc acttgcatt acccaacga tcaacctgc caggcctct cgttcctcc cccgccccc accgccgct accgccaat tgatttggg cgaatttga ggagccgcc cccacaaga gggcgatta cactgtgaa ccacttcct cgactctct caaagccgc agctcagag tcgctccag cagtgagga ggtcttcca agattaaat tttgttcgc atgttttta aattttgtt aacgtttga tgtgttcag tttagtttt tgttctttg ttcattctt ttgtactta attcgtttg cgttcaaat aaatgttaa aaaaatac
- >TC001771 RC
gacctctta acagattag ggagggcaa ttttttatt taattgata aaaacaatt acaaatggc tttcaatca gcacaatct ccaaaccat gcacgagcg cgaagttgt tggaaagtg cattgcgga gctccgcac tcacgtttt tgaatacta cgaggcttt ggatgtttt gcataaaca ttacgagat aaatctcaa caaaccaaa acgcgatcc gcaatcaaa gctcgttga gataccaga ggatggccc tattccctc aaccagtcg cgggaacgc tgctgatgc gagcttgaa tcctgttga accggacaa agacgagga atttgaggc ttacgttgg ggccaatga taataattc cgatagtag caccagcca cagtgctta cgatgagca ggaaaaccg cagatacgc ctctctgct gagccaaga actgaagtc caaattgat aactcacgc cactttcat ggccgcaag acaacgcag aggacttaa ggacgaaga ccctgaagt gactactga agaggtgcc tccacgact tttcagggt gcccgaaca tttgccacc gctgcctcc tctcgctcc tgtgccaca atttcgtcc tgttccact tgcattacc caacgatca acctgccag gcctctcgt tcctccccc gcccccacc gccgctacc gccaattga tttgggcga atttgagga gccgccccc acaagaggg cgattacac tgtgaacca cttcctcga ctctctcaa agccgcagc tcagagtcg ctccagcag tgaggaggt cttccaaga ttaaatttt gttcgcatg tttttaaat tttgttaac gtttgatgt gttcagttt agtttttgt tctttgttc attcttttg tacttaatt cgtttgcgt tcaaataaa tgttaaaaa aatac
- >TC001771 RD
tttcgcaaa agtcaaatt ttcatcaaa gtttttgtc tacgttatt tgtgagtat tttcatagt taaagttgt ttattctga tattattac acaagaatc gacgctggg ttgggccta gtacaccag agcgacgaa atttgctac ttccaggaa gtccaggga agtgcaaaa acatcgaaa atggcacta gcacggaaa actcaattt gttatcaat tgctcgcaa tccgattac tacattcat tgcattatt aattctgga gtcatctta gacgaagac atggagtta attttgacg tatttcgca agtgatgat cgttacagt caggagatt aagttatcg gttttgcaa aaaataatt ggttgtgtc tctttggct ggaacgatc tttttggtg gaaagaaaa taccccctt tatcttttg ccgatttcg gccacacca atgtttttg atgatgctt aaaacaatg cgccgctcc attcaacaa aaaacacaa caacgtaac ttcgaggtt tttgttgaa acgactggt aagatcgcg aaactaaat cgcgtgatt tgtgaatat ttcaagcac agaaacgtg ccaagtact gaaacttcc gatattctc aatccgtac acgactgca atgaagaag ttgctcgaa gtagttttc atggaggaa accactttg ttatacaaa ctacgactt tgcgtacgc gaatttgtc ggttacgtg cctttcctt aaaaaggac tatacccat gtggactat attgaaata cataccttg accagccta aagattcgc aatattaat gactgtgtt attcaactg aaacaactc tgtgatatc tatgtatta ctagcttcc aattgccta acttcgttg tcggtggct ttgagcccg cgagtttgg tccatatat agagttgat ctggagtat atcttggag gtactcatt ccgagaatg agaaaactc tctgaacaa tgttacagc gctataagc aaaaaattc gatgattta aaattctat aacttgaac cgaggccag gtcaaaaag cgcatgatg aagatcaaa cgccataat gggcagaca aaactgtat ttggggact tgcgatata atggtatac atttcagac atcattgag aagtctgaa atagtgtac aaaaaattg caggaggtg agtcttgtt tttatattg attaaacga cgagggagg aggtataca aagattata taattgttt tgtgcaaga cgaactggg tataaaatg tagtttgct tgataaaca tttta
