Status
Please wait ...
Gene information
General information
TC001771 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7761526..7765746
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001771
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00303

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks - cuticles: eggs lost,
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      remarks: animals lost,
      Pupal morphology
      remarks: animals lost,
      Adult morphology
      remarks: animals lost,
      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001771 RA
        MALARKTQF VINCSQSDY YIHCIINSG VILDEDMEL 
        ILTYFASDD RYSQEIKLS VLQKIIGCV SLAGTIFLV 
        ERKYPLYLL PISATPMFL MMLKTMRRS IQQKTQQRN 
        FEVFVETTG KIAKLNRVI CEYFKHRNV PSTETSDIL 
        NPYTTAMKK LLEVVFMEE TTLLYKLRL CVREFVGYV 
        PFLKKDYTH VDYIEIHTL TSLKIRNIN DCVIQLKQL 
        CDIYVLLAS NCLTSLSVA LSPRVWSIY RVDLEYILE 
        VLIPRMRKL SEQCYSAIS KKFDDLKFY NLNRGQVKK 
        RMMKIKRHN GQTKLYLGT CDIMVYISD IIEKSEIVY 
        KKLQEHNLQ TMHEREVVG KCIAELRTH VFEYYEALD 
        VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG 
        NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS 
        AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG 
        LVSISENSA EICNFLFSI FQKDEDPEV TTEEVPPRL 
        FRVPEHLPP LPPLAPVPQ FRPVPLALP NDQPARPLV 
        PPPPPPPLP PIDLGEFEE PPPQEGDYT VNHFLDSLK 
        AAAQSRSSS EEVFQD
      • >TC001771 RB
        MAFNQHNLQ TMHEREVVG KCIAELRTH VFEYYEALD 
        VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG 
        NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS 
        AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG 
        LVSISENSA EICNFLFSI FQKDEDPEV TTEEVPPRL 
        FRVPEHLPP LPPLAPVPQ FRPVPLALP NDQPARPLV 
        PPPPPPPLP PIDLGEFEE PPPQEGDYT VNHFLDSLK 
        AAAQSRSSS EEVFQD
      • >TC001771 RC
        MAFNQHNLQ TMHEREVVG KCIAELRTH VFEYYEALD 
        VLHKHYEIN LNKPKRDPQ SKLVEIPED GPIPSTSRG 
        NAADASLNP VEPDKDEEF EAYVGANDN NSDSSTSHS 
        AYDEQENRR YASLLSQEL KSKLITHAT FMAARQRRG 
        LKDEDPEVT TEEVPPRLF RVPEHLPPL PPLAPVPQF 
        RPVPLALPN DQPARPLVP PPPPPPLPP IDLGEFEEP 
        PPQEGDYTV NHFLDSLKA AAQSRSSSE EVFQD
      • >TC001771 RD
        MALARKTQF VINCSQSDY YIHCIINSG VILDEDMEL 
        ILTYFASDD RYSQEIKLS VLQKIIGCV SLAGTIFLV 
        ERKYPLYLL PISATPMFL MMLKTMRRS IQQKTQQRN 
        FEVFVETTG KIAKLNRVI CEYFKHRNV PSTETSDIL 
        NPYTTAMKK LLEVVFMEE TTLLYKLRL CVREFVGYV 
        PFLKKDYTH VDYIEIHTL TSLKIRNIN DCVIQLKQL 
        CDIYVLLAS NCLTSLSVA LSPRVWSIY RVDLEYILE 
        VLIPRMRKL SEQCYSAIS KKFDDLKFY NLNRGQVKK 
        RMMKIKRHN GQTKLYLGT CDIMVYISD IIEKSEIVY 
        KKLQEVSLV FILIKRRGR RYTKII
      CDS sequence
      • >TC001771 RA
        atggcacta gcacggaaa actcaattt gttatcaat 
        tgctcgcaa tccgattac tacattcat tgcattatt 
        aattctgga gtcatctta gacgaagac atggagtta 
        attttgacg tatttcgca agtgatgat cgttacagt 
        caggagatt aagttatcg gttttgcaa aaaataatt 
        ggttgtgtc tctttggct ggaacgatc tttttggtg 
        gaaagaaaa taccccctt tatcttttg ccgatttcg 
        gccacacca atgtttttg atgatgctt aaaacaatg 
        cgccgctcc attcaacaa aaaacacaa caacgtaac 
        ttcgaggtt tttgttgaa acgactggt aagatcgcg 
        aaactaaat cgcgtgatt tgtgaatat ttcaagcac 
        agaaacgtg ccaagtact gaaacttcc gatattctc 
        aatccgtac acgactgca atgaagaag ttgctcgaa 
        gtagttttc atggaggaa accactttg ttatacaaa 
        ctacgactt tgcgtacgc gaatttgtc ggttacgtg 
        cctttcctt aaaaaggac tatacccat gtggactat 
        attgaaata cataccttg accagccta aagattcgc 
        aatattaat gactgtgtt attcaactg aaacaactc 
        tgtgatatc tatgtatta ctagcttcc aattgccta 
        acttcgttg tcggtggct ttgagcccg cgagtttgg 
        tccatatat agagttgat ctggagtat atcttggag 
        gtactcatt ccgagaatg agaaaactc tctgaacaa 
        tgttacagc gctataagc aaaaaattc gatgattta 
        aaattctat aacttgaac cgaggccag gtcaaaaag 
        cgcatgatg aagatcaaa cgccataat gggcagaca 
        aaactgtat ttggggact tgcgatata atggtatac 
        atttcagac atcattgag aagtctgaa atagtgtac 
        aaaaaattg caggagcac aatctccaa accatgcac 
        gagcgcgaa gttgttgga aagtgcatt gcggagctc 
        cgcactcac gtttttgaa tactacgag gctttggat 
        gttttgcat aaacattac gagataaat ctcaacaaa 
        ccaaaacgc gatccgcaa tcaaagctc gttgagata 
        ccagaggat ggccctatt ccctcaacc agtcgcggg 
        aacgctgct gatgcgagc ttgaatcct gttgaaccg 
        gacaaagac gaggaattt gaggcttac gttggggcc 
        aatgataat aattccgat agtagcacc agccacagt 
        gcttacgat gagcaggaa aaccgcaga tacgcctct 
        ctgctgagc caagaactg aagtccaaa ttgataact 
        cacgccact ttcatggcc gcaagacaa cgcagagga 
        cttgtcagt atttcagaa aatagtgcc gagatctgc 
        aactttctc ttttcaatt tttcagaag gacgaagac 
        cctgaagtg actactgaa gaggtgcct ccacgactt 
        ttcagggtg cccgaacat ttgccaccg ctgcctcct 
        ctcgctcct gtgccacaa tttcgtcct gttccactt 
        gcattaccc aacgatcaa cctgccagg cctctcgtt 
        cctcccccg cccccaccg ccgctaccg ccaattgat 
        ttgggcgaa tttgaggag ccgccccca caagagggc 
        gattacact gtgaaccac ttcctcgac tctctcaaa 
        gccgcagct cagagtcgc tccagcagt gaggaggtc 
        ttccaagat taa
      • >TC001771 RB
        atggctttc aatcagcac aatctccaa accatgcac 
        gagcgcgaa gttgttgga aagtgcatt gcggagctc 
        cgcactcac gtttttgaa tactacgag gctttggat 
        gttttgcat aaacattac gagataaat ctcaacaaa 
        ccaaaacgc gatccgcaa tcaaagctc gttgagata 
        ccagaggat ggccctatt ccctcaacc agtcgcggg 
        aacgctgct gatgcgagc ttgaatcct gttgaaccg 
        gacaaagac gaggaattt gaggcttac gttggggcc 
        aatgataat aattccgat agtagcacc agccacagt 
        gcttacgat gagcaggaa aaccgcaga tacgcctct 
        ctgctgagc caagaactg aagtccaaa ttgataact 
        cacgccact ttcatggcc gcaagacaa cgcagagga 
        cttgtcagt atttcagaa aatagtgcc gagatctgc 
        aactttctc ttttcaatt tttcagaag gacgaagac 
        cctgaagtg actactgaa gaggtgcct ccacgactt 
        ttcagggtg cccgaacat ttgccaccg ctgcctcct 
        ctcgctcct gtgccacaa tttcgtcct gttccactt 
        gcattaccc aacgatcaa cctgccagg cctctcgtt 
        cctcccccg cccccaccg ccgctaccg ccaattgat 
        ttgggcgaa tttgaggag ccgccccca caagagggc 
        gattacact gtgaaccac ttcctcgac tctctcaaa 
        gccgcagct cagagtcgc tccagcagt gaggaggtc 
        ttccaagat taa
      • >TC001771 RC
        atggctttc aatcagcac aatctccaa accatgcac 
        gagcgcgaa gttgttgga aagtgcatt gcggagctc 
        cgcactcac gtttttgaa tactacgag gctttggat 
        gttttgcat aaacattac gagataaat ctcaacaaa 
        ccaaaacgc gatccgcaa tcaaagctc gttgagata 
        ccagaggat ggccctatt ccctcaacc agtcgcggg 
        aacgctgct gatgcgagc ttgaatcct gttgaaccg 
        gacaaagac gaggaattt gaggcttac gttggggcc 
        aatgataat aattccgat agtagcacc agccacagt 
        gcttacgat gagcaggaa aaccgcaga tacgcctct 
        ctgctgagc caagaactg aagtccaaa ttgataact 
        cacgccact ttcatggcc gcaagacaa cgcagagga 
        cttaaggac gaagaccct gaagtgact actgaagag 
        gtgcctcca cgacttttc agggtgccc gaacatttg 
        ccaccgctg cctcctctc gctcctgtg ccacaattt 
        cgtcctgtt ccacttgca ttacccaac gatcaacct 
        gccaggcct ctcgttcct cccccgccc ccaccgccg 
        ctaccgcca attgatttg ggcgaattt gaggagccg 
        cccccacaa gagggcgat tacactgtg aaccacttc 
        ctcgactct ctcaaagcc gcagctcag agtcgctcc 
        agcagtgag gaggtcttc caagattaa
      • >TC001771 RD
        atggcacta gcacggaaa actcaattt gttatcaat 
        tgctcgcaa tccgattac tacattcat tgcattatt 
        aattctgga gtcatctta gacgaagac atggagtta 
        attttgacg tatttcgca agtgatgat cgttacagt 
        caggagatt aagttatcg gttttgcaa aaaataatt 
        ggttgtgtc tctttggct ggaacgatc tttttggtg 
        gaaagaaaa taccccctt tatcttttg ccgatttcg 
        gccacacca atgtttttg atgatgctt aaaacaatg 
        cgccgctcc attcaacaa aaaacacaa caacgtaac 
        ttcgaggtt tttgttgaa acgactggt aagatcgcg 
        aaactaaat cgcgtgatt tgtgaatat ttcaagcac 
        agaaacgtg ccaagtact gaaacttcc gatattctc 
        aatccgtac acgactgca atgaagaag ttgctcgaa 
        gtagttttc atggaggaa accactttg ttatacaaa 
        ctacgactt tgcgtacgc gaatttgtc ggttacgtg 
        cctttcctt aaaaaggac tatacccat gtggactat 
        attgaaata cataccttg accagccta aagattcgc 
        aatattaat gactgtgtt attcaactg aaacaactc 
        tgtgatatc tatgtatta ctagcttcc aattgccta 
        acttcgttg tcggtggct ttgagcccg cgagtttgg 
        tccatatat agagttgat ctggagtat atcttggag 
        gtactcatt ccgagaatg agaaaactc tctgaacaa 
        tgttacagc gctataagc aaaaaattc gatgattta 
        aaattctat aacttgaac cgaggccag gtcaaaaag 
        cgcatgatg aagatcaaa cgccataat gggcagaca 
        aaactgtat ttggggact tgcgatata atggtatac 
        atttcagac atcattgag aagtctgaa atagtgtac 
        aaaaaattg caggaggtg agtcttgtt tttatattg 
        attaaacga cgagggagg aggtataca aagattata 
        taa
      mRNA sequence
      • >TC001771 RA
        tttcgcaaa agtcaaatt ttcatcaaa gtttttgtc 
        tacgttatt tgtgagtat tttcatagt taaagttgt 
        ttattctga tattattac acaagaatc gacgctggg 
        ttgggccta gtacaccag agcgacgaa atttgctac 
        ttccaggaa gtccaggga agtgcaaaa acatcgaaa 
        atggcacta gcacggaaa actcaattt gttatcaat 
        tgctcgcaa tccgattac tacattcat tgcattatt 
        aattctgga gtcatctta gacgaagac atggagtta 
        attttgacg tatttcgca agtgatgat cgttacagt 
        caggagatt aagttatcg gttttgcaa aaaataatt 
        ggttgtgtc tctttggct ggaacgatc tttttggtg 
        gaaagaaaa taccccctt tatcttttg ccgatttcg 
        gccacacca atgtttttg atgatgctt aaaacaatg 
        cgccgctcc attcaacaa aaaacacaa caacgtaac 
        ttcgaggtt tttgttgaa acgactggt aagatcgcg 
        aaactaaat cgcgtgatt tgtgaatat ttcaagcac 
        agaaacgtg ccaagtact gaaacttcc gatattctc 
        aatccgtac acgactgca atgaagaag ttgctcgaa 
        gtagttttc atggaggaa accactttg ttatacaaa 
        ctacgactt tgcgtacgc gaatttgtc ggttacgtg 
        cctttcctt aaaaaggac tatacccat gtggactat 
        attgaaata cataccttg accagccta aagattcgc 
        aatattaat gactgtgtt attcaactg aaacaactc 
        tgtgatatc tatgtatta ctagcttcc aattgccta 
        acttcgttg tcggtggct ttgagcccg cgagtttgg 
        tccatatat agagttgat ctggagtat atcttggag 
        gtactcatt ccgagaatg agaaaactc tctgaacaa 
        tgttacagc gctataagc aaaaaattc gatgattta 
        aaattctat aacttgaac cgaggccag gtcaaaaag 
        cgcatgatg aagatcaaa cgccataat gggcagaca 
        aaactgtat ttggggact tgcgatata atggtatac 
        atttcagac atcattgag aagtctgaa atagtgtac 
        aaaaaattg caggagcac aatctccaa accatgcac 
        gagcgcgaa gttgttgga aagtgcatt gcggagctc 
        cgcactcac gtttttgaa tactacgag gctttggat 
        gttttgcat aaacattac gagataaat ctcaacaaa 
        ccaaaacgc gatccgcaa tcaaagctc gttgagata 
        ccagaggat ggccctatt ccctcaacc agtcgcggg 
        aacgctgct gatgcgagc ttgaatcct gttgaaccg 
        gacaaagac gaggaattt gaggcttac gttggggcc 
        aatgataat aattccgat agtagcacc agccacagt 
        gcttacgat gagcaggaa aaccgcaga tacgcctct 
        ctgctgagc caagaactg aagtccaaa ttgataact 
        cacgccact ttcatggcc gcaagacaa cgcagagga 
        cttgtcagt atttcagaa aatagtgcc gagatctgc 
        aactttctc ttttcaatt tttcagaag gacgaagac 
        cctgaagtg actactgaa gaggtgcct ccacgactt 
        ttcagggtg cccgaacat ttgccaccg ctgcctcct 
        ctcgctcct gtgccacaa tttcgtcct gttccactt 
        gcattaccc aacgatcaa cctgccagg cctctcgtt 
        cctcccccg cccccaccg ccgctaccg ccaattgat 
        ttgggcgaa tttgaggag ccgccccca caagagggc 
        gattacact gtgaaccac ttcctcgac tctctcaaa 
        gccgcagct cagagtcgc tccagcagt gaggaggtc 
        ttccaagat taaattttg ttcgcatgt ttttaaatt 
        ttgttaacg tttgatgtg ttcagttta gtttttgtt 
        ctttgttca ttcttttgt acttaattc gtttgcgtt 
        caaataaat gttaaaaaa atac
      • >TC001771 RB
        gacctctta acagattag ggagggcaa ttttttatt 
        taattgata aaaacaatt acaaatggc tttcaatca 
        gcacaatct ccaaaccat gcacgagcg cgaagttgt 
        tggaaagtg cattgcgga gctccgcac tcacgtttt 
        tgaatacta cgaggcttt ggatgtttt gcataaaca 
        ttacgagat aaatctcaa caaaccaaa acgcgatcc 
        gcaatcaaa gctcgttga gataccaga ggatggccc 
        tattccctc aaccagtcg cgggaacgc tgctgatgc 
        gagcttgaa tcctgttga accggacaa agacgagga 
        atttgaggc ttacgttgg ggccaatga taataattc 
        cgatagtag caccagcca cagtgctta cgatgagca 
        ggaaaaccg cagatacgc ctctctgct gagccaaga 
        actgaagtc caaattgat aactcacgc cactttcat 
        ggccgcaag acaacgcag aggacttgt cagtatttc 
        agaaaatag tgccgagat ctgcaactt tctcttttc 
        aatttttca gaaggacga agaccctga agtgactac 
        tgaagaggt gcctccacg acttttcag ggtgcccga 
        acatttgcc accgctgcc tcctctcgc tcctgtgcc 
        acaatttcg tcctgttcc acttgcatt acccaacga 
        tcaacctgc caggcctct cgttcctcc cccgccccc 
        accgccgct accgccaat tgatttggg cgaatttga 
        ggagccgcc cccacaaga gggcgatta cactgtgaa 
        ccacttcct cgactctct caaagccgc agctcagag 
        tcgctccag cagtgagga ggtcttcca agattaaat 
        tttgttcgc atgttttta aattttgtt aacgtttga 
        tgtgttcag tttagtttt tgttctttg ttcattctt 
        ttgtactta attcgtttg cgttcaaat aaatgttaa 
        aaaaatac
      • >TC001771 RC
        gacctctta acagattag ggagggcaa ttttttatt 
        taattgata aaaacaatt acaaatggc tttcaatca 
        gcacaatct ccaaaccat gcacgagcg cgaagttgt 
        tggaaagtg cattgcgga gctccgcac tcacgtttt 
        tgaatacta cgaggcttt ggatgtttt gcataaaca 
        ttacgagat aaatctcaa caaaccaaa acgcgatcc 
        gcaatcaaa gctcgttga gataccaga ggatggccc 
        tattccctc aaccagtcg cgggaacgc tgctgatgc 
        gagcttgaa tcctgttga accggacaa agacgagga 
        atttgaggc ttacgttgg ggccaatga taataattc 
        cgatagtag caccagcca cagtgctta cgatgagca 
        ggaaaaccg cagatacgc ctctctgct gagccaaga 
        actgaagtc caaattgat aactcacgc cactttcat 
        ggccgcaag acaacgcag aggacttaa ggacgaaga 
        ccctgaagt gactactga agaggtgcc tccacgact 
        tttcagggt gcccgaaca tttgccacc gctgcctcc 
        tctcgctcc tgtgccaca atttcgtcc tgttccact 
        tgcattacc caacgatca acctgccag gcctctcgt 
        tcctccccc gcccccacc gccgctacc gccaattga 
        tttgggcga atttgagga gccgccccc acaagaggg 
        cgattacac tgtgaacca cttcctcga ctctctcaa 
        agccgcagc tcagagtcg ctccagcag tgaggaggt 
        cttccaaga ttaaatttt gttcgcatg tttttaaat 
        tttgttaac gtttgatgt gttcagttt agtttttgt 
        tctttgttc attcttttg tacttaatt cgtttgcgt 
        tcaaataaa tgttaaaaa aatac
      • >TC001771 RD
        tttcgcaaa agtcaaatt ttcatcaaa gtttttgtc 
        tacgttatt tgtgagtat tttcatagt taaagttgt 
        ttattctga tattattac acaagaatc gacgctggg 
        ttgggccta gtacaccag agcgacgaa atttgctac 
        ttccaggaa gtccaggga agtgcaaaa acatcgaaa 
        atggcacta gcacggaaa actcaattt gttatcaat 
        tgctcgcaa tccgattac tacattcat tgcattatt 
        aattctgga gtcatctta gacgaagac atggagtta 
        attttgacg tatttcgca agtgatgat cgttacagt 
        caggagatt aagttatcg gttttgcaa aaaataatt 
        ggttgtgtc tctttggct ggaacgatc tttttggtg 
        gaaagaaaa taccccctt tatcttttg ccgatttcg 
        gccacacca atgtttttg atgatgctt aaaacaatg 
        cgccgctcc attcaacaa aaaacacaa caacgtaac 
        ttcgaggtt tttgttgaa acgactggt aagatcgcg 
        aaactaaat cgcgtgatt tgtgaatat ttcaagcac 
        agaaacgtg ccaagtact gaaacttcc gatattctc 
        aatccgtac acgactgca atgaagaag ttgctcgaa 
        gtagttttc atggaggaa accactttg ttatacaaa 
        ctacgactt tgcgtacgc gaatttgtc ggttacgtg 
        cctttcctt aaaaaggac tatacccat gtggactat 
        attgaaata cataccttg accagccta aagattcgc 
        aatattaat gactgtgtt attcaactg aaacaactc 
        tgtgatatc tatgtatta ctagcttcc aattgccta 
        acttcgttg tcggtggct ttgagcccg cgagtttgg 
        tccatatat agagttgat ctggagtat atcttggag 
        gtactcatt ccgagaatg agaaaactc tctgaacaa 
        tgttacagc gctataagc aaaaaattc gatgattta 
        aaattctat aacttgaac cgaggccag gtcaaaaag 
        cgcatgatg aagatcaaa cgccataat gggcagaca 
        aaactgtat ttggggact tgcgatata atggtatac 
        atttcagac atcattgag aagtctgaa atagtgtac 
        aaaaaattg caggaggtg agtcttgtt tttatattg 
        attaaacga cgagggagg aggtataca aagattata 
        taattgttt tgtgcaaga cgaactggg tataaaatg 
        tagtttgct tgataaaca tttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY