Status
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Gene information
General information
TC001770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7767320..7771066
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001770
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00302

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 44.44% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001770 RA
      MKLLLAVAT IITLLQPYD ATPARIVCY FSNWAIYRP 
      GIGRYAQED LPVDLCTHI VYSFIGVND ADWSVLVID 
      PELDVDQNG FRNFTNLKK THPKVKLQV AVGGWAEGG 
      SKYSAMVAE KSRRDAFIR SVISFMNQY EFDGFDLDW 
      EYPGAADRG GSFSDKDKF FYFVEELRR AFDKEGKNW 
      EITMAVPIA KFRLQEGYH VPELCELLD AIHVMSYDL 
      RGNWAGFAD SHSPLYKRP HDQWAYEKL NVNDGLQLW 
      VDYGCSPKK LIVGIPFYG RTFTLSNSN TNYNPGTYI 
      NKEAGGGSP GPYTNATGF LAYYEICPE ILNKDSGWT 
      KKWDEHGKV PYAYKGNQW VGYEDPESV QIKMDWIKS 
      KGYGGAMTW AIDMDDFHG LCGPKNPLI NILYANMNS 
      YSVPEPTIS TTPRPEWAR PPSTPSADG SKPVQTTPK 
      PGQWVPEKS TSTTQRTTT VSTTEAPPA PAVSNPQNE 
      VDGSVAPKE CKQDFMPHE LCDKYYRCV HGKPTEFAC 
      RPGTVFHTQ SNICDWPEH ADREKCKKI EQ
    CDS sequence
    • >TC001770 RA
      atgaagctg ctcctagct gtagcaaca ataataacc 
      ctactgcaa ccctacgac gcaaccccg gcccgcatc 
      gtttgctac ttcagcaac tgggctatt taccggcct 
      gggatcggg cgctacgcc caggaggac ctccctgtg 
      gacctatgc acccacata gtctactct ttcatcggc 
      gtaaatgac gcagattgg agcgtcctg gtcatagac 
      ccagagctg gacgtggac caaaacggc ttccgcaac 
      tttaccaat ttgaagaaa acgcacccc aaagtcaaa 
      ctccaagtt gcagtgggg ggctgggcc gagggcggc 
      tcgaaatac tcggccatg gtggcggag aagtcgcga 
      agggatgcg tttatcagg agcgtaatc agtttcatg 
      aatcaatac gagttcgat gggtttgat ctggactgg 
      gaatacccc ggggcggct gaccgtggg ggtagtttc 
      agcgataag gacaagttc ttttacttt gtggaggag 
      ctgagacgg gcgtttgat aaggaaggg aaaaattgg 
      gaaattaca atggcggtc ccgattgcc aaattccgg 
      ctgcaggaa ggttatcac gtcccggaa ttatgcgaa 
      ttgttggat gccattcat gtcatgtcg tacgatctg 
      agggggaat tgggccggg tttgccgat tctcatagt 
      cctctgtat aagaggcct catgaccag tgggcttac 
      gagaaactg aacgtcaac gatggtttg caactttgg 
      gtcgattac ggctgctcc ccgaaaaaa ctaatcgtt 
      ggaataccg ttctatggc cgaactttc acccttagt 
      aacagcaac accaactac aaccccgga acttacata 
      aataaagaa gcagggggt ggaagcccc ggaccatac 
      acaaacgcc accggcttt cttgcgtat tacgagatt 
      tgtcccgaa attctgaac aaagacagc ggttggacg 
      aaaaaatgg gacgaacac ggcaaagtc ccttacgcg 
      tacaagggc aatcagtgg gtcgggtac gaggacccc 
      gaaagtgtc caaatcaag atggactgg atcaaatca 
      aagggctac ggtggggcc atgacttgg gctattgac 
      atggacgac tttcatggt ctttgtggt cccaaaaac 
      cctcttatt aatattctt tacgccaac atgaactcg 
      tactcggtc ccagaaccc accatttcg accactccg 
      aggcctgag tgggcgcgc cctccgagt accccatct 
      gccgatggc tcaaaaccg gtgcaaacg accccaaaa 
      ccgggacaa tgggtgccc gagaagtcc acatcgacg 
      actcaaagg accacaacg gtgagtacg actgaggct 
      ccgccagcc ccagctgtc tcaaatccg caaaacgaa 
      gttgatgga tcagtggcc cccaaggag tgtaaacag 
      gactttatg ccacatgag ctttgcgat aagtactat 
      cgttgcgta catgggaaa cccacggaa tttgcatgc 
      agaccaggg actgttttc catactcag agcaatatt 
      tgtgattgg ccggaacat gccgatcgt gaaaaatgc 
      aaaaagatt gaacaataa
    mRNA sequence
    • >TC001770 RA
      ccacgctgc atattaatc agttaatca atgtcttgt 
      catgagaaa tgaacagtt aatatgcag atggttgaa 
      gagccacta tacagtttt atgatttcg aaaggtttg 
      cctcttggt tcgatgaca taaaaaacg atcaagaag 
      agccgcata taaaagttg aaggaacgc tgggactaa 
      catttattc gactgatct cgtcctagc ggcactaga 
      aaaaatgaa gctgctcct agctgtagc aacaataat 
      aaccctact gcaacccta cgacgcaac cccggcccg 
      catcgtttg ctacttcag caactgggc tatttaccg 
      gcctgggat cgggcgcta cgcccagga ggacctccc 
      tgtggacct atgcaccca catagtcta ctctttcat 
      cggcgtaaa tgacgcaga ttggagcgt cctggtcat 
      agacccaga gctggacgt ggaccaaaa cggcttccg 
      caactttac caatttgaa gaaaacgca ccccaaagt 
      caaactcca agttgcagt ggggggctg ggccgaggg 
      cggctcgaa atactcggc catggtggc ggagaagtc 
      gcgaaggga tgcgtttat caggagcgt aatcagttt 
      catgaatca atacgagtt cgatgggtt tgatctgga 
      ctgggaata ccccggggc ggctgaccg tgggggtag 
      tttcagcga taaggacaa gttctttta ctttgtgga 
      ggagctgag acgggcgtt tgataagga agggaaaaa 
      ttgggaaat tacaatggc ggtcccgat tgccaaatt 
      ccggctgca ggaaggtta tcacgtccc ggaattatg 
      cgaattgtt ggatgccat tcatgtcat gtcgtacga 
      tctgagggg gaattgggc cgggtttgc cgattctca 
      tagtcctct gtataagag gcctcatga ccagtgggc 
      ttacgagaa actgaacgt caacgatgg tttgcaact 
      ttgggtcga ttacggctg ctccccgaa aaaactaat 
      cgttggaat accgttcta tggccgaac tttcaccct 
      tagtaacag caacaccaa ctacaaccc cggaactta 
      cataaataa agaagcagg gggtggaag ccccggacc 
      atacacaaa cgccaccgg ctttcttgc gtattacga 
      gatttgtcc cgaaattct gaacaaaga cagcggttg 
      gacgaaaaa atgggacga acacggcaa agtccctta 
      cgcgtacaa gggcaatca gtgggtcgg gtacgagga 
      ccccgaaag tgtccaaat caagatgga ctggatcaa 
      atcaaaggg ctacggtgg ggccatgac ttgggctat 
      tgacatgga cgactttca tggtctttg tggtcccaa 
      aaaccctct tattaatat tctttacgc caacatgaa 
      ctcgtactc ggtcccaga acccaccat ttcgaccac 
      tccgaggcc tgagtgggc gcgccctcc gagtacccc 
      atctgccga tggctcaaa accggtgca aacgacccc 
      aaaaccggg acaatgggt gcccgagaa gtccacatc 
      gacgactca aaggaccac aacggtgag tacgactga 
      ggctccgcc agccccagc tgtctcaaa tccgcaaaa 
      cgaagttga tggatcagt ggcccccaa ggagtgtaa 
      acaggactt tatgccaca tgagctttg cgataagta 
      ctatcgttg cgtacatgg gaaacccac ggaatttgc 
      atgcagacc agggactgt tttccatac tcagagcaa 
      tatttgtga ttggccgga acatgccga tcgtgaaaa 
      atgcaaaaa gattgaaca ataataatg tttgactga 
      tttaacata agatatcct atgtattat tttattata 
      aataaaata ttttttgaa tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY