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Gene information
General information
TC001765 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7799594..7856099
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001765
    iBeetle screen iB_00300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection



    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001765 RA
      MKRPLFTLF VAVAAATAL TAAASSSND IIKTFTDPN 
      VQAFNHLVV DKNTGRVYI GAVNRLYQL SPDLDLVIS 
      EKTGPEMDS DDCSVLECT SAFSKKLTD NVNKALVID 
      YTTSRLISC GSLFQGICS VRNLDNIAE GVRVVREAV 
      VANNATAST VAFIAPGPP NPPVSQVMY VGVTFTLGS 
      PYRSEVPAV SSRSLDKDK MFTIAQTAV TTGTRMFVN 
      SLARERYPI TYVYGFASE GFSYFLTTQ MRHTGSNQF 
      ISKLVRVCH DDENYYSYT EIPIDCISD SQGGRKYNL 
      VQAAYVGKA GSDLASDLG ITAQDDVLF GVFSEGDPP 
      SSNKPSDLS ALCVYPLKA IRRKFMQNI KHCFSGKGQ 
      RGLDFISPS LQCVSTKLQ TIGEDFCGL DVNTPLGGE 
      QPISAVPVL LFTTRLTAV AATSTGDFT VVFIGTATG 
      HLKKVVVES RSSALEYGD LTVEEGSPV NPDLYFDSQ 
      LMHLYVMTE KKVSKVKVQ ECSVYRSCL ECLGAKDPY 
      CGWCSLENK CSLRSDCQD AAKDPLYWI SYKSGRCTT 
      ITSVIPNQL QRTTARTLD LIIDNLPSL SGHFLCAFT 
      VLDKPLITN ATRKGSGVN CTTPRTDLL PSIPPGQHH 
      FTAKLSVRM TSGPDFVAT NFTFFDCNT YSSCTQCVS 
      SSFPCDWCV DGHRCTHDT AENCRNDIL VTGVSRAGP 
      SYRSGPAFC PTINATVGN SPEILVASG IKKAIKVKV 
      HIIGQFIVQ TRFVCQFNI EGRVTSVNA QLLGDTIYC 
      DPMEFSYTS RAPNITATF AVIWGGSKP LDNPDNIHI 
      VIYRCRDMA DNCGMCLAL AKKYDCGWC QSSDRCEVK 
      DQCERGTAV WLNRSQTCP NPEVISFSP ELGPWEGGT 
      NITIQGINL GKTFQDIYT GVMIAGINC QPYEHLYIK 
      TKQIVCKVD GPGINEARH GPVLVRVED YRGESKTHF 
      NFVDPVITG IAPKYGPRS GGTMLQITG KYMNAGSNI 
      QAFLDDLPC RILSTDQNQ ALCITSASD KTGPKKLRM 
      KFDKGDRYF EKEYFEYVE DPIIESAES GVASQIKVP 
      KGIPAGGIK ISVTGKNLA YIQKPQMYV YYEQKMFIS 
      ECTVQSNTS MTCSSPVIE ADESKLDAD NPLKLEYGF 
      RMDNVSGVQ NLTLNKDFS PFLLFPNPV FIPFDKEVK 
      YYKSEYLNI NGQNIDRAC QESDVEVRI GNSFCNVTS 
      LSRQQLTCR PPETQPPAL NSEGQPNGE ALPEVTVIV 
      GGSLRFNIG HLSYSPAQG LNGPISKPT MIVGIAIVV 
      VILVIFSGF LIAYRRKST ESNRVLKNM QEQMDILEL 
      RVAAECKEA FAELQTEMT DLTGDLTSG GIPFLDYRT 
      YAMKILFPN VDDHAVLQW ERPELIRKE KGLRCFGQL 
      IMNKTFLLL FIRTLESNR YFSMRDKVN VASLIMVTL 
      QSKMEYCTD ILKTLLAEL IEKCMEGKS HPKLLLRRT 
      ESVAEKMLS AWFTFLLYK FLRECAGEP LFMLFRAVK 
      QQVDKGPVD AITSEARYS LSEEKLIRQ SIDFKPMTV 
      YVSISQQTV FIGGLDHNT ENVPVKVLD CDTISQVKE 
      KSLDTIYRA TPYSQRPRK DDLDLEWRT GTSGRLILY 
      DEDSTNKTE GEWKRRNTL NHYRVPDGG CLNLVSKQS 
      SIYNLSIFS EKNEKSHKY ETLNLSKFS SASPPLSRA 
      TSPLNHDQD QGYKVWHLV KHHDNDNQK EGERGNKMV 
      SEIYLTRLL ATKGTLQKF VDDLFETIF STAHRGSAL 
      PLAIKYMFD FLDDQALQH GISDPEVVH TWKSNSLPL 
      RFWVNIIKN PNFVFDIHK SNIVDSCLS VVAQTFMDS 
      CSTSDHRLG KDSPSSKLL YAKDIPCYK EWVERYYSD 
      IKMMSAISD QDMNAMLAE ESRLHTTEF NTNCALHEL 
      YTYAMKYNE QLTVTLEED EFSQKQRLH YKLEQVHNI 
      MSDQGQP
    CDS sequence
    • >TC001765 RA
      atgaagcgg ccgcttttt acattattt gtggctgtg 
      gccgctgcc acagcattg acagcagcc gcctcatcg 
      agcaacgac attatcaaa actttcacc gatccaaat 
      gtacaagca ttcaaccat cttgtggtg gataaaaat 
      acagggcgt gtatatatc ggcgctgtg aaccgttta 
      tatcaatta tcacccgat ctagactta gtaatatca 
      gaaaaaacg ggaccagag atggattca gatgactgt 
      tcggtgctc gaatgtaca tctgctttt agtaaaaaa 
      ttaaccgat aacgtaaac aaggccctc gtgatcgac 
      tacacgacg tcgcggctc atctcctgc ggcagcctg 
      ttccagggg atttgctcg gttcggaac ctggacaac 
      atcgccgaa ggggtgcgg gtggtccgc gaggccgtc 
      gtggcgaac aacgcgacg gcgtcaacg gtcgcgttc 
      atcgcccca gggccgccc aaccccccg gtctcgcaa 
      gtgatgtac gtgggggtg acgttcacg ttggggtcg 
      ccttaccgg tccgaggtg cccgccgtc agcagccgg 
      tcgctggac aaggacaaa atgttcacg atcgcgcaa 
      acggcggtc accactggc acgcgaatg ttcgtcaat 
      tcgctcgct cgcgaacga tatccgata acgtacgtc 
      tacggattc gcctcggaa gggttcagc tactttctg 
      accactcag atgaggcac accggctcg aaccagttc 
      ataagcaag ctagtgcgc gtttgccac gacgatgag 
      aactactac tcctacacg gagattccc atcgactgc 
      atcagcgac tcgcaaggc ggccgcaag tacaacctg 
      gtgcaggcc gcctacgtg ggcaaggcg ggctcagac 
      ctggcctcg gatttgggg attacggcc caggacgac 
      gtcctcttc ggggtgttc agtgaagga gacccgccg 
      agttccaac aaaccctcc gatttgtcg gctctctgc 
      gtctatccg ctgaaagcc atcaggagg aagtttatg 
      cagaacatc aagcactgc ttcagtggc aaaggccaa 
      cggggcctg gatttcatc tcgccaagt cttcagtgt 
      gtctccacg aaattgcaa accatcgga gaggacttc 
      tgtgggtta gatgtgaac acgcctttg ggaggagag 
      caacccatc tctgctgtt ccagtgcta cttttcacc 
      acgaggctg acggcagtc gctgccacc tccacaggt 
      gattttacc gtcgtcttc ataggcacc gccacggga 
      catctcaaa aaggtggtg gtggagtcg cgctcctcc 
      gcgctggaa tatggcgac ttgacggtg gaggagggc 
      tcacctgta aaccccgat ttgtatttc gactcgcag 
      ctgatgcat ttgtacgtg atgacggag aaaaaggtg 
      tcgaaagta aaagtgcag gaatgcagc gtgtacaga 
      agttgcttg gaatgcctc ggtgcgaaa gatccatac 
      tgtgggtgg tgttcgttg gaaaacaag tgtagcctc 
      cgctccgac tgtcaagac gctgccaaa gatccttta 
      tattggatt agctataag agcggaagg tgtacaaca 
      ataacttcc gttataccg aatcaactc caaagaacc 
      accgctcga acgctggac ctcatcatt gacaattta 
      ccgagtcta agcggccat ttcttatgt gcgttcact 
      gttcttgat aaaccatta ataacaaac gctacgagg 
      aagggatcc ggagttaat tgcaccaca ccaaggacc 
      gatctcctc ccgtcaatt cctcccggc caacatcac 
      tttactgcc aaactatca gtccgaatg acatccgga 
      ccggatttt gtcgcaaca aatttcaca ttcttcgat 
      tgcaacacg tatagttcc tgtacgcaa tgcgtatct 
      tcctccttt ccctgcgac tggtgcgtc gacggacac 
      agatgcacc cacgatacg gcggaaaat tgccggaac 
      gacatcctc gtaacaggt gttagccgt gccggccca 
      agctaccgg tccgggccg gcgttttgt cccacgatc 
      aacgcaacg gtgggtaat tccccggaa atcctcgtg 
      gcttcagga ataaaaaaa gcaatcaaa gtcaaagtg 
      catatcatc ggtcaattc atagtccaa acgcgtttt 
      gtttgccaa tttaacatc gaggggcgc gtcactagt 
      gtgaacgca caattgctg ggcgatacg atttattgc 
      gacccgatg gagttttcg tacacctcg cgcgccccc 
      aacatcacg gccaccttc gccgtgatc tggggcggc 
      tccaagccg ctcgacaac cccgacaac atccacatt 
      gttatctac cgatgccgc gacatggcc gataactgc 
      ggcatgtgc ctggctttg gcgaaaaaa tacgactgc 
      ggctggtgc cagagctcc gatcggtgc gaagtcaag 
      gatcagtgc gagagaggc accgcggtg tggctcaac 
      cggagccag acatgccct aacccggag gtgatctcc 
      ttcagcccg gagttaggg ccgtgggaa ggcggcacc 
      aatatcacg atccagggg atcaacctc gggaaaacc 
      ttccaggat atttacacc ggagtcatg atcgcggga 
      atcaattgc cagccgtac gaacatctc tacatcaaa 
      acgaagcaa atcgtgtgc aaagtcgac gggcccgga 
      attaatgaa gcgcgacac ggacccgtg ctggtccgg 
      gtggaggac tacaggggc gaatccaaa acacatttt 
      aatttcgtc gatccggtc atcaccgga atcgcgccc 
      aagtacggg cccaggtcc ggggggacc atgctccaa 
      atcaccggg aagtacatg aacgccggg agcaatatc 
      caggcgttc ctcgacgac ttgccttgt cggatactt 
      tcgaccgac caaaaccaa gccctgtgc attaccagt 
      gcgagtgat aagaccggt ccgaaaaaa ttgcgtatg 
      aaattcgat aaaggcgat cggtatttc gagaaagaa 
      tatttcgag tacgtggaa gatccgatt atagaatcg 
      gcggaatcg ggagttgct agtcagatt aaggttcct 
      aaaggcata ccagctgga ggcattaag atttcagtt 
      accggaaaa aatctcgcg tacatacaa aagccacaa 
      atgtatgtt tactacgag caaaaaatg ttcattagc 
      gaatgtacg gtgcagagt aatactagt atgacatgt 
      agtagtcca gttatagaa gcagacgag tcaaaatta 
      gacgcggat aatcccttg aaattagag tatggattt 
      aggatggac aatgtttcc ggagtgcag aatctcacg 
      ctgaataaa gatttctca cctttcttg ctgttccca 
      aatcccgtc tttattccc ttcgataaa gaagtgaaa 
      tactacaag tcggagtat ctcaatatc aacggccag 
      aatatagac cgtgcgtgt caagagtcg gacgttgag 
      gtccgaatt gggaatagt ttctgcaac gtaacttct 
      ctctcccga caacagcta acatgtcgc ccacccgaa 
      acccaacct ccggccctc aacagcgaa ggccaaccc 
      aacggtgaa gccttacct gaggttaca gtgatagtg 
      ggtggatca ttacgtttt aacatcggt catttgtca 
      tactcaccc gctcaaggt ctcaacggt cctatttcc 
      aaacccacg atgattgta ggcattgca atcgtcgtt 
      gtcattctc gtaatattc agcggtttc ttaatcgcc 
      tatcggcga aagtcaacc gaaagtaac cgggttctg 
      aaaaatatg caagaacag atggacatc ctcgaatta 
      cgagtggcc gccgagtgt aaggaggcc ttcgccgaa 
      ctgcagacc gaaatgact gacctgacc ggagacctg 
      acttccggc ggtattcca tttttggac taccggact 
      tacgctatg aagatcctg ttcccgaac gtggacgac 
      cacgctgtc ctgcagtgg gaacggccc gagctcatc 
      cgaaaagag aagggacta cgctgtttc ggccaatta 
      attatgaac aaaaccttc ctcctgctc ttcattcgt 
      acgctagaa agcaaccga tatttctcg atgcgagat 
      aaagtgaac gtggcgtcg ctcatcatg gtgacgctc 
      caaagcaag atggagtat tgcaccgac atcttgaag 
      acgctcctt gccgaactg atcgagaag tgcatggag 
      gggaaaagc cacccgaag ttgctgcta agacgtacg 
      gaaagtgtc gccgagaaa atgctaagt gcgtggttt 
      acattcctg ttgtacaag ttcttgcgt gagtgtgcc 
      ggggagccg ctgttcatg ttgttccga gcggtgaag 
      cagcaagtg gataagggg cccgtcgat gctatcact 
      tccgaagcg agatactca ctcagcgaa gagaaactc 
      atcagacaa tcaatagat tttaagcct atgactgta 
      tatgtttcc atttctcaa caaactgtg tttattggt 
      ggtctcgac cacaacact gagaatgtc cctgtaaag 
      gttttggac tgtgataca ataagtcaa gtgaaggag 
      aaatctctc gatacgatt tatagagca accccatac 
      tcgcaaaga cctagaaaa gacgattta gatttagag 
      tggcgtacg gggacctcc ggccgtctt attttgtac 
      gacgaagac agcacgaac aaaaccgag ggcgagtgg 
      aagcggaga aacaccctc aaccactac cgcgtgccc 
      gacggcggg tgcctaaac ctagtgtca aaacaaagt 
      tccatttat aatttaagt attttctcc gagaagaac 
      gagaagtcc cacaagtac gaaacccta aacctaagt 
      aaattcagc tcggcgagc ccgccgctg agccgcgcc 
      accagcccg ctgaaccac gaccaagac caagggtac 
      aaggtgtgg catctggtc aagcaccac gacaacgac 
      aaccaaaag gagggcgag agagggaac aagatggtc 
      tcggagatc tacctgacg cgccttctg gcgacgaag 
      gggacgctg caaaagttc gtcgacgat ttgttcgag 
      acgatattc agcacggcc caccgagga tcggcgcta 
      ccacttgcc atcaaatac atgttcgat tttctcgac 
      gatcaggcg ctgcagcat ggcatttct gatcccgag 
      gtcgtgcac acttggaag agtaattcc ttgccgctc 
      aggttttgg gtcaatatt attaaaaat cctaatttt 
      gtattcgac atacacaag tcaaatatc gtggattcg 
      tgtttatct gtagttgct caaactttc atggactcc 
      tgctccaca tccgatcat aggttaggt aaagactcg 
      ccaagttcc aagttactg tatgccaag gacattcca 
      tgttacaag gagtgggta gagaggtat tactccgat 
      atcaagatg atgtcggcg atttctgac caagatatg 
      aacgcgatg ttggcggaa gagtctagg cttcacaca 
      actgaattt aatacgaat tgtgcatta cacgagctt 
      tatacgtac gcgatgaaa tacaacgag cagctgact 
      gtgaccctt gaagaggac gagttctcg cagaagcag 
      cggttgcat tacaaatta gaacaggtt cataatata 
      atgtccgat caggggcaa ccgtga
    mRNA sequence
    • >TC001765 RA
      tagcttcag tttttgaac aagtctaat aacgcggtt 
      gttgtgttg aagggtgtt tcgttgctg ggtaaagtt 
      ggtgatacc ggtattgtg ttcaattct aagtaatac 
      tggccaaat ggtagagac tgggcgact gaaaccggg 
      gctatagct gagtttgga ccgccagca gagtgcacg 
      tcgaagctt agagcgata ttgtggacg ggactgatc 
      ctaacctaa atagtttcc acagatgca gtgaagttc 
      agaccagta tgttgtcca cctagagct ccggtcgtg 
      gatgagaca agtgcacgg cggttgtga gacacccgt 
      gtcgtgatt tcgggtccg aactgtgga tgtaacctt 
      aaagtgtaa tgtgtgtaa gtgggttgc actgagttt 
      cacttccta tgggaaatg gacactata taggtccca 
      tgccttgaa gttcctgga gagctggtg ccgctatga 
      agcggccgc tttttacat tatttgtgg ctgtggccg 
      ctgccacag cattgacag cagccgcct catcgagca 
      acgacatta tcaaaactt tcaccgatc caaatgtac 
      aagcattca accatcttg tggtggata aaaatacag 
      ggcgtgtat atatcggcg ctgtgaacc gtttatatc 
      aattatcac ccgatctag acttagtaa tatcagaaa 
      aaacgggac cagagatgg attcagatg actgttcgg 
      tgctcgaat gtacatctg cttttagta aaaaattaa 
      ccgataacg taaacaagg ccctcgtga tcgactaca 
      cgacgtcgc ggctcatct cctgcggca gcctgttcc 
      aggggattt gctcggttc ggaacctgg acaacatcg 
      ccgaagggg tgcgggtgg tccgcgagg ccgtcgtgg 
      cgaacaacg cgacggcgt caacggtcg cgttcatcg 
      ccccagggc cgcccaacc ccccggtct cgcaagtga 
      tgtacgtgg gggtgacgt tcacgttgg ggtcgcctt 
      accggtccg aggtgcccg ccgtcagca gccggtcgc 
      tggacaagg acaaaatgt tcacgatcg cgcaaacgg 
      cggtcacca ctggcacgc gaatgttcg tcaattcgc 
      tcgctcgcg aacgatatc cgataacgt acgtctacg 
      gattcgcct cggaagggt tcagctact ttctgacca 
      ctcagatga ggcacaccg gctcgaacc agttcataa 
      gcaagctag tgcgcgttt gccacgacg atgagaact 
      actactcct acacggaga ttcccatcg actgcatca 
      gcgactcgc aaggcggcc gcaagtaca acctggtgc 
      aggccgcct acgtgggca aggcgggct cagacctgg 
      cctcggatt tggggatta cggcccagg acgacgtcc 
      tcttcgggg tgttcagtg aaggagacc cgccgagtt 
      ccaacaaac cctccgatt tgtcggctc tctgcgtct 
      atccgctga aagccatca ggaggaagt ttatgcaga 
      acatcaagc actgcttca gtggcaaag gccaacggg 
      gcctggatt tcatctcgc caagtcttc agtgtgtct 
      ccacgaaat tgcaaacca tcggagagg acttctgtg 
      ggttagatg tgaacacgc ctttgggag gagagcaac 
      ccatctctg ctgttccag tgctacttt tcaccacga 
      ggctgacgg cagtcgctg ccacctcca caggtgatt 
      ttaccgtcg tcttcatag gcaccgcca cgggacatc 
      tcaaaaagg tggtggtgg agtcgcgct cctccgcgc 
      tggaatatg gcgacttga cggtggagg agggctcac 
      ctgtaaacc ccgatttgt atttcgact cgcagctga 
      tgcatttgt acgtgatga cggagaaaa aggtgtcga 
      aagtaaaag tgcaggaat gcagcgtgt acagaagtt 
      gcttggaat gcctcggtg cgaaagatc catactgtg 
      ggtggtgtt cgttggaaa acaagtgta gcctccgct 
      ccgactgtc aagacgctg ccaaagatc ctttatatt 
      ggattagct ataagagcg gaaggtgta caacaataa 
      cttccgtta taccgaatc aactccaaa gaaccaccg 
      ctcgaacgc tggacctca tcattgaca atttaccga 
      gtctaagcg gccatttct tatgtgcgt tcactgttc 
      ttgataaac cattaataa caaacgcta cgaggaagg 
      gatccggag ttaattgca ccacaccaa ggaccgatc 
      tcctcccgt caattcctc ccggccaac atcacttta 
      ctgccaaac tatcagtcc gaatgacat ccggaccgg 
      attttgtcg caacaaatt tcacattct tcgattgca 
      acacgtata gttcctgta cgcaatgcg tatcttcct 
      cctttccct gcgactggt gcgtcgacg gacacagat 
      gcacccacg atacggcgg aaaattgcc ggaacgaca 
      tcctcgtaa caggtgtta gccgtgccg gcccaagct 
      accggtccg ggccggcgt tttgtccca cgatcaacg 
      caacggtgg gtaattccc cggaaatcc tcgtggctt 
      caggaataa aaaaagcaa tcaaagtca aagtgcata 
      tcatcggtc aattcatag tccaaacgc gttttgttt 
      gccaattta acatcgagg ggcgcgtca ctagtgtga 
      acgcacaat tgctgggcg atacgattt attgcgacc 
      cgatggagt tttcgtaca cctcgcgcg cccccaaca 
      tcacggcca ccttcgccg tgatctggg gcggctcca 
      agccgctcg acaaccccg acaacatcc acattgtta 
      tctaccgat gccgcgaca tggccgata actgcggca 
      tgtgcctgg ctttggcga aaaaatacg actgcggct 
      ggtgccaga gctccgatc ggtgcgaag tcaaggatc 
      agtgcgaga gaggcaccg cggtgtggc tcaaccgga 
      gccagacat gccctaacc cggaggtga tctccttca 
      gcccggagt tagggccgt gggaaggcg gcaccaata 
      tcacgatcc aggggatca acctcggga aaaccttcc 
      aggatattt acaccggag tcatgatcg cgggaatca 
      attgccagc cgtacgaac atctctaca tcaaaacga 
      agcaaatcg tgtgcaaag tcgacgggc ccggaatta 
      atgaagcgc gacacggac ccgtgctgg tccgggtgg 
      aggactaca ggggcgaat ccaaaacac attttaatt 
      tcgtcgatc cggtcatca ccggaatcg cgcccaagt 
      acgggccca ggtccgggg ggaccatgc tccaaatca 
      ccgggaagt acatgaacg ccgggagca atatccagg 
      cgttcctcg acgacttgc cttgtcgga tactttcga 
      ccgaccaaa accaagccc tgtgcatta ccagtgcga 
      gtgataaga ccggtccga aaaaattgc gtatgaaat 
      tcgataaag gcgatcggt atttcgaga aagaatatt 
      tcgagtacg tggaagatc cgattatag aatcggcgg 
      aatcgggag ttgctagtc agattaagg ttcctaaag 
      gcataccag ctggaggca ttaagattt cagttaccg 
      gaaaaaatc tcgcgtaca tacaaaagc cacaaatgt 
      atgtttact acgagcaaa aaatgttca ttagcgaat 
      gtacggtgc agagtaata ctagtatga catgtagta 
      gtccagtta tagaagcag acgagtcaa aattagacg 
      cggataatc ccttgaaat tagagtatg gatttagga 
      tggacaatg tttccggag tgcagaatc tcacgctga 
      ataaagatt tctcacctt tcttgctgt tcccaaatc 
      ccgtcttta ttcccttcg ataaagaag tgaaatact 
      acaagtcgg agtatctca atatcaacg gccagaata 
      tagaccgtg cgtgtcaag agtcggacg ttgaggtcc 
      gaattggga atagtttct gcaacgtaa cttctctct 
      cccgacaac agctaacat gtcgcccac ccgaaaccc 
      aacctccgg ccctcaaca gcgaaggcc aacccaacg 
      gtgaagcct tacctgagg ttacagtga tagtgggtg 
      gatcattac gttttaaca tcggtcatt tgtcatact 
      cacccgctc aaggtctca acggtccta tttccaaac 
      ccacgatga ttgtaggca ttgcaatcg tcgttgtca 
      ttctcgtaa tattcagcg gtttcttaa tcgcctatc 
      ggcgaaagt caaccgaaa gtaaccggg ttctgaaaa 
      atatgcaag aacagatgg acatcctcg aattacgag 
      tggccgccg agtgtaagg aggccttcg ccgaactgc 
      agaccgaaa tgactgacc tgaccggag acctgactt 
      ccggcggta ttccatttt tggactacc ggacttacg 
      ctatgaaga tcctgttcc cgaacgtgg acgaccacg 
      ctgtcctgc agtgggaac ggcccgagc tcatccgaa 
      aagagaagg gactacgct gtttcggcc aattaatta 
      tgaacaaaa ccttcctcc tgctcttca ttcgtacgc 
      tagaaagca accgatatt tctcgatgc gagataaag 
      tgaacgtgg cgtcgctca tcatggtga cgctccaaa 
      gcaagatgg agtattgca ccgacatct tgaagacgc 
      tccttgccg aactgatcg agaagtgca tggagggga 
      aaagccacc cgaagttgc tgctaagac gtacggaaa 
      gtgtcgccg agaaaatgc taagtgcgt ggtttacat 
      tcctgttgt acaagttct tgcgtgagt gtgccgggg 
      agccgctgt tcatgttgt tccgagcgg tgaagcagc 
      aagtggata aggggcccg tcgatgcta tcacttccg 
      aagcgagat actcactca gcgaagaga aactcatca 
      gacaatcaa tagatttta agcctatga ctgtatatg 
      tttccattt ctcaacaaa ctgtgttta ttggtggtc 
      tcgaccaca acactgaga atgtccctg taaaggttt 
      tggactgtg atacaataa gtcaagtga aggagaaat 
      ctctcgata cgatttata gagcaaccc catactcgc 
      aaagaccta gaaaagacg atttagatt tagagtggc 
      gtacgggga cctccggcc gtcttattt tgtacgacg 
      aagacagca cgaacaaaa ccgagggcg agtggaagc 
      ggagaaaca ccctcaacc actaccgcg tgcccgacg 
      gcgggtgcc taaacctag tgtcaaaac aaagttcca 
      tttataatt taagtattt tctccgaga agaacgaga 
      agtcccaca agtacgaaa ccctaaacc taagtaaat 
      tcagctcgg cgagcccgc cgctgagcc gcgccacca 
      gcccgctga accacgacc aagaccaag ggtacaagg 
      tgtggcatc tggtcaagc accacgaca acgacaacc 
      aaaaggagg gcgagagag ggaacaaga tggtctcgg 
      agatctacc tgacgcgcc ttctggcga cgaagggga 
      cgctgcaaa agttcgtcg acgatttgt tcgagacga 
      tattcagca cggcccacc gaggatcgg cgctaccac 
      ttgccatca aatacatgt tcgattttc tcgacgatc 
      aggcgctgc agcatggca tttctgatc ccgaggtcg 
      tgcacactt ggaagagta attccttgc cgctcaggt 
      tttgggtca atattatta aaaatccta attttgtat 
      tcgacatac acaagtcaa atatcgtgg attcgtgtt 
      tatctgtag ttgctcaaa ctttcatgg actcctgct 
      ccacatccg atcataggt taggtaaag actcgccaa 
      gttccaagt tactgtatg ccaaggaca ttccatgtt 
      acaaggagt gggtagaga ggtattact ccgatatca 
      agatgatgt cggcgattt ctgaccaag atatgaacg 
      cgatgttgg cggaagagt ctaggcttc acacaactg 
      aatttaata cgaattgtg cattacacg agctttata 
      cgtacgcga tgaaataca acgagcagc tgactgtga 
      cccttgaag aggacgagt tctcgcaga agcagcggt 
      tgcattaca aattagaac aggttcata atataatgt 
      ccgatcagg ggcaaccgt gatactggc ctgatctga 
      tgcgtccgt cagcgcctc cgatatctc ctgtggtcc 
      ccctgcagc cctcagcac ctcaggaac tggcctgct 
      gatagaccg ttgcagcca cttatacat atccatcat 
      gaaactttc tctgtgtgt tgttggatt gctgtcatg 
      cagttatag tcgttctgt gtcggggtc ggtgcgcac 
      tcagtgact tcaagtgcc tctggtagg attgagtgg 
      tgtactgtc tccataaat aagtgtata tttttatga 
      taacgttat tctgtttat gctttgctg tgctgacac 
      tcagcagta gtccatata tcgatattt gcgtgaata 
      tcaactatg cttttttga taactgtat gatgaatgt 
      accttatta tctctgtac agtttaata tttttatat 
      ttagttgaa tgcaatcaa gacgattgt cttccgcat 
      tgaaggaag cagcagggt gtagtgttt atttgcgac 
      ttttcaatc atcgatgtc tcaacctat acatactgt 
      gcacgggtt gtgtcagtg tgatttgtg tacgtgtaa 
      taaggctgt gtacgttta cgttaaagc agacagcag 
      tactgtgcc gctagtgac agtgttgct gcgataatt 
      cgttcacct tcgttcctt cgttccaca tcatttgtt 
      attgtaaat tacatccaa atggaaatg ttcaggctg 
      taaactatc atttactaa acgtctgta aataagttc 
      ggggatcca aaactttgc attttaata acgttcttt 
      attacatta tcctttcat ttgtttact cattatctc 
      atacgaatt gtaaccgtc ctttaaata attattggg 
      aaaaaggaa agcaatgaa tcctcaact tagctatgg 
      acagctgcg catatcacg tccagtgta gcagtgcga 
      tttttatta tttatatct attttatat atttttact 
      tttttaaat tttaacata agttttacc aaaccaatg 
      cttctggac aatttgcgt agaaaaatt ttgtatata 
      ctaagtgtg tgtgcacga tgtattcat agtactgtg 
      tgtatactc gattgcatt tgcaataat atgaatggt 
      tatgtaata ctatatgtc attataaaa gactgaatg 
      gagttattt caaatactt a
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