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Gene information
General information
TC001761 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 7618151..7622135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001761
    iBeetle screen iB_00299
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001761 RA
      MASICKIVQ SSLTRRKPL SFCKFFHNA SPKSRPNRL 
      ASVVCAVGG TVVFYTAFK CRQTQSIHA SQLKEEESK 
      VVKLTSREK RFIRFASVE FDGQLYMTP QDFLESVVE 
      AEPRPRLKR KILTAKELE KIKDSTPPL NQGSPRFFR 
      TLRDKGIIS YTEYLFLLS ILTKPQSGF RIAFNMFDT 
      DGNERVDKN EFLVMEKIF SHAWKGKRG IASEENEPI 
      LPIQEHYVD DEQGLQRRH TVDTTLIVH FFGPKGNNE 
      LNFENFKQF MLHLQTEVL ELEFSEFSK GLPTISEVD 
      FAKIMLRYT YLDTDEYDM YLDRLLDRI KETKGITFE 
      EFNVFCQFL NNLEDFTIA MRMYTLADH PISKDEFHR 
      AVKICTGTS LSPHLVHTV FAIFDVDGD GLLSYREFI 
      AIMKDRLHR GFKSYTKNE GWEAFKSCI KQEMKNPT
    CDS sequence
    • >TC001761 RA
      atggctagc atttgcaag atcgtgcaa agttcacta 
      actcgaaga aagccgcta agtttttgt aagtttttc 
      cacaatgct agtcccaag tcacgacca aaccgtttg 
      gcttctgtc gtgtgtgcg gtcggtggg actgtcgtc 
      ttctacacg gcctttaaa tgccgacaa acccaaagc 
      atacatgcc tcccaattg aaagaggag gaatcaaaa 
      gttgtcaaa ttaacatcg cgggaaaag agatttata 
      cgcttcgct tcggtcgaa ttcgacgga caactctac 
      atgaccccg caggacttc ctcgaatcg gtggtcgaa 
      gccgagccc cgaccccgt cttaaacgc aaaatcctg 
      acggcgaaa gagctggaa aaaatcaaa gactcgact 
      ccgccgctc aaccaaggc tcgccccgg tttttccgc 
      actttgcgc gacaaaggc attatttca tacaccgag 
      tatttgttc cttctctca attttgaca aagccacaa 
      tccggcttc cgcatcgca tttaacatg ttcgacacc 
      gatggtaac gagcgagtc gacaaaaac gagtttctt 
      gtgatggag aaaatcttc agccacgcg tggaagggc 
      aagcggggg atcgccagc gaggagaac gagccgatc 
      ctcccgatc caggagcac tacgttgat gatgagcag 
      gggctccag cgaaggcac accgtggac accaccctc 
      attgtgcac ttcttcggg ccgaaggga aacaacgaa 
      ctcaacttc gagaatttt aaacagttc atgctccac 
      ctccaaacc gaggttctg gagctggag tttagcgag 
      ttctcgaag ggacttccg accatttct gaggtcgat 
      tttgccaaa atcatgctg cgttacact tatctagac 
      acggacgag tacgacatg tacttggac cgactactg 
      gacaggatt aaggagacc aaaggcatt acattcgag 
      gagttcaac gtgttttgc cagttcctc aacaatttg 
      gaagacttc accatcgca atgcgcatg tataccttg 
      gcggaccac ccgatttcc aaagatgag ttccatcgg 
      gccgtgaaa atatgcact ggtactagt ctaagcccg 
      catctagtc cacacagta tttgcgatt ttcgacgtc 
      gatggagac ggactgttg agttaccgc gagtttatc 
      gcaattatg aaagaccgc ctgcatcga ggcttcaaa 
      tcatacacc aaaaatgaa ggttgggag gcattcaaa 
      agttgcatc aagcaagaa atgaagaac ccgacatag
    mRNA sequence
    • >TC001761 RA
      ctccagtta tcagctgtt atcttaaaa aatggctag 
      catttgcaa gatcgtgca aagttcact aactcgaag 
      aaagccgct aagtttttg taagttttt ccacaatgc 
      tagtcccaa gtcacgacc aaaccgttt ggcttctgt 
      cgtgtgtgc ggtcggtgg gactgtcgt cttctacac 
      ggcctttaa atgccgaca aacccaaag catacatgc 
      ctcccaatt gaaagagga ggaatcaaa agttgtcaa 
      attaacatc gcgggaaaa gagatttat acgcttcgc 
      ttcggtcga attcgacgg acaactcta catgacccc 
      gcaggactt cctcgaatc ggtggtcga agccgagcc 
      ccgaccccg tcttaaacg caaaatcct gacggcgaa 
      agagctgga aaaaatcaa agactcgac tccgccgct 
      caaccaagg ctcgccccg gtttttccg cactttgcg 
      cgacaaagg cattatttc atacaccga gtatttgtt 
      ccttctctc aattttgac aaagccaca atccggctt 
      ccgcatcgc atttaacat gttcgacac cgatggtaa 
      cgagcgagt cgacaaaaa cgagtttct tgtgatgga 
      gaaaatctt cagccacgc gtggaaggg caagcgggg 
      gatcgccag cgaggagaa cgagccgat cctcccgat 
      ccaggagca ctacgttga tgatgagca ggggctcca 
      gcgaaggca caccgtgga caccaccct cattgtgca 
      cttcttcgg gccgaaggg aaacaacga actcaactt 
      cgagaattt taaacagtt catgctcca cctccaaac 
      cgaggttct ggagctgga gtttagcga gttctcgaa 
      gggacttcc gaccatttc tgaggtcga ttttgccaa 
      aatcatgct gcgttacac ttatctaga cacggacga 
      gtacgacat gtacttgga ccgactact ggacaggat 
      taaggagac caaaggcat tacattcga ggagttcaa 
      cgtgttttg ccagttcct caacaattt ggaagactt 
      caccatcgc aatgcgcat gtatacctt ggcggacca 
      cccgatttc caaagatga gttccatcg ggccgtgaa 
      aatatgcac tggtactag tctaagccc gcatctagt 
      ccacacagt atttgcgat tttcgacgt cgatggaga 
      cggactgtt gagttaccg cgagtttat cgcaattat 
      gaaagaccg cctgcatcg aggcttcaa atcatacac 
      caaaaatga aggttggga ggcattcaa aagttgcat 
      caagcaaga aatgaagaa cccgacata gaaactaag 
      tatttattt gtgataacc gcattttca cattggaga 
      gttctagta ttgccatct agtcagaat ttttatgac 
      gttcctgtc attttcacg gattgttac tagtcgt