Status
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Gene information
General information
TC001754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7963234..7969548
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001754
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00297

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 25.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001754 RA
      MSDDLHNSV LLSTFSSGN KKYRVIFHK GTIVWGNGK 
      PPIDQESLP LENVISVQH NYTRNRHPS DDAILDPNH 
      FTINYAKKD EENRWKPHS LTLGHSDPL QVSSWVKTL 
      QNHLQYFKR PKRLLLFVN PFGGKRNAL KIYEKYGKP 
      LFQTAGVDV TVNVSQRKN QIRDFVLNH SLDMFDSIA 
      CVGGDGTVS ELFNGLVLR ECKNLGIDA DDIEQDLPK 
      PKIPIGIIP GGSTDTIVY CLHGTIDPT TAVLNIIFG 
      ETLGLDLVS VYDESSLLR LYASVLSYG YLGDVAYHS 
      DKYRWMGPN RYNYSGFKK LMRNRGYEG EVAFFSEVG 
      DLKSSKCYE NCERCLSRR NNCDKTEKQ WRTLKGKFF 
      MISGANITC SCNRSPQGI APYSHLGDG NVHLVLVRH 
      TSILNNLKL LLRLSSNNQ AVDDLPFVE TYSAREFCF 
      RAINGPSRW NCDGEVQHQ TNIRAKVRC QLLTIFSRG 
      TPKPIQATR RCCGCY
    CDS sequence
    • >TC001754 RA
      atgtctgat gatttacac aattcagta cttctgagc 
      acattctcg agtggcaat aagaaatac cgtgtgatt 
      tttcacaaa ggaacaatc gtctggggc aacggcaag 
      ccaccgatc gaccaagaa tcgttgcct ctggaaaat 
      gttatctca gtccagcac aactacacg agaaatcgg 
      cacccttca gacgacgca atcctcgac cccaaccac 
      ttcacaata aactacgcg aagaaagac gaggagaat 
      cgatggaag ccccacagc ctcaccctg ggccactcg 
      gaccctctg caggtctcc tcgtgggtc aagacgctg 
      cagaaccac ctccaatat tttaagcga ccgaagcgc 
      ctgctcctg ttcgtgaac ccgttcggg ggcaagcgc 
      aacgcgctg aagatttac gagaagtac ggcaagccc 
      ctgttccaa accgcgggg gtggacgtc actgtcaac 
      gtctcgcag cggaagaac cagatccgc gacttcgtc 
      ctgaaccac agcctggac atgttcgac tcgatcgcg 
      tgcgtgggc ggcgacggc accgtctcg gagctgttc 
      aacgggctg gtgctccgc gagtgcaag aacctgggg 
      atcgacgcc gacgacatc gagcaagac ctgcccaag 
      ccgaagatt cccatcggg atcatccca gggggaagt 
      accgacacg atcgtctac tgtctgcac gggactatt 
      gacccgacg acggccgtc ttgaatatc attttcggc 
      gagactttg ggtctggat ctggtgtcg gtttacgac 
      gagagcagt ttattgcgg ttgtatgcc agtgttttg 
      agctatggg tatttgggc gacgtcgct tatcatagt 
      gataagtac cggtggatg ggtcccaat cggtacaat 
      tattcaggt tttaaaaaa ctgatgcgg aaccgaggt 
      tacgaggga gaagtagcg tttttctcc gaagtaggc 
      gatttgaaa agttcgaaa tgttacgag aattgcgaa 
      cgctgcctg tccagaaga aacaattgt gataaaacc 
      gagaagcaa tggcgcact ttaaagggg aaatttttc 
      atgataagt ggtgctaac ataacatgc tcgtgcaat 
      cgcagccct cagggaatc gcaccttac agtcacttg 
      ggtgacggc aacgtccac ttagtttta gttcgacat 
      acttcgatt ttgaacaat ttgaaacta ctattacgc 
      ttgtcgagt aataaccag gctgttgat gacttgcca 
      tttgttgaa acttactct gctagggaa ttctgcttt 
      cgtgcaatc aatggccct agcaggtgg aactgtgat 
      ggggaagtg cagcaccag acgaatatt cgtgcaaaa 
      gtacgttgc caattgctc acgattttt agccgcgga 
      actccgaag ccaatccaa gccacgagg aggtgctgc 
      gggtgctac taa
    mRNA sequence
    • >TC001754 RA
      caaccatta atgtttaat taaatttta tggcagaaa 
      caagtgtag acgggggca tttacccca cccaaacaa 
      caataaacg ctatttttg caagtattt accgcagat 
      aagaaaaac aaatttgaa atgtggcgc atgtgcagt 
      gttaccatc tccatatgt ctgaaaaaa atgagtgac 
      agtgacagc agtttgaca gtgacgttt ggtgcgata 
      aaagggctt taaaaacag taaacatgt ttgaatttt 
      ccctcagta ttatttccc aagcagtat gactaaaaa 
      aatgtgatg tatcatttc gaattattc aagcgtgaa 
      ttgcttcca ctccgattt ttacataat gtctgatga 
      tttacacaa ttcagtact tctgagcac attctcgag 
      tggcaataa gaaataccg tgtgatttt tcacaaagg 
      aacaatcgt ctggggcaa cggcaagcc accgatcga 
      ccaagaatc gttgcctct ggaaaatgt tatctcagt 
      ccagcacaa ctacacgag aaatcggca cccttcaga 
      cgacgcaat cctcgaccc caaccactt cacaataaa 
      ctacgcgaa gaaagacga ggagaatcg atggaagcc 
      ccacagcct caccctggg ccactcgga ccctctgca 
      ggtctcctc gtgggtcaa gacgctgca gaaccacct 
      ccaatattt taagcgacc gaagcgcct gctcctgtt 
      cgtgaaccc gttcggggg caagcgcaa cgcgctgaa 
      gatttacga gaagtacgg caagcccct gttccaaac 
      cgcgggggt ggacgtcac tgtcaacgt ctcgcagcg 
      gaagaacca gatccgcga cttcgtcct gaaccacag 
      cctggacat gttcgactc gatcgcgtg cgtgggcgg 
      cgacggcac cgtctcgga gctgttcaa cgggctggt 
      gctccgcga gtgcaagaa cctggggat cgacgccga 
      cgacatcga gcaagacct gcccaagcc gaagattcc 
      catcgggat catcccagg gggaagtac cgacacgat 
      cgtctactg tctgcacgg gactattga cccgacgac 
      ggccgtctt gaatatcat tttcggcga gactttggg 
      tctggatct ggtgtcggt ttacgacga gagcagttt 
      attgcggtt gtatgccag tgttttgag ctatgggta 
      tttgggcga cgtcgctta tcatagtga taagtaccg 
      gtggatggg tcccaatcg gtacaatta ttcaggttt 
      taaaaaact gatgcggaa ccgaggtta cgagggaga 
      agtagcgtt tttctccga agtaggcga tttgaaaag 
      ttcgaaatg ttacgagaa ttgcgaacg ctgcctgtc 
      cagaagaaa caattgtga taaaaccga gaagcaatg 
      gcgcacttt aaaggggaa atttttcat gataagtgg 
      tgctaacat aacatgctc gtgcaatcg cagccctca 
      gggaatcgc accttacag tcacttggg tgacggcaa 
      cgtccactt agttttagt tcgacatac ttcgatttt 
      gaacaattt gaaactact attacgctt gtcgagtaa 
      taaccaggc tgttgatga cttgccatt tgttgaaac 
      ttactctgc tagggaatt ctgctttcg tgcaatcaa 
      tggccctag caggtggaa ctgtgatgg ggaagtgca 
      gcaccagac gaatattcg tgcaaaagt acgttgcca 
      attgctcac gatttttag ccgcggaac tccgaagcc 
      aatccaagc cacgaggag gtgctgcgg gtgctacta 
      agccttagc tgccttatg ttaattgcc caatgttag 
      ttcagtatt ggttattgg ttgtgtaac ggaaaacta 
      tattttgta attgtaatt gctcacatg tattaaaag 
      tgatatgaa tttgacacg ttatgttcc gtcattcca 
      gttacgatg tattatttt tttattttg taacagtat 
      ttaggtgtt gaggccgtc tatacttaa attaccgat 
      cgctctata tgttagaga ctgactctc attccatgt 
      tcgtggtga aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY