Status
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Gene information
General information
TC001752 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7976440..7981727
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001752
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00296

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001752 RA
      MDQNTRDNK DRNIDWSRF GLGSETAQR VRANTSGFV 
      DLSNDYGAG GHQASGANE LFAEEQGDV PWWKSNFFI 
      SQPVLFGTW DGVFTSCLI NIFGVIVFL RSGWIVGQA 
      GVISAVLIV LATVFIALI SVLSAVGIC ERCRIESGG 
      VYFVIAHVL GSRFGGSLG LLYCFGQAV GCALNVLGF 
      GESIAELIG FGEYKIAVR IIASAAVLF LSVINVAGV 
      KWVIKLQFI LLLILLLAG LDFMVGSFV HTDVENGFD 
      GWLGGNLHA NLWSEYKDG YSWFTVFGV FFPTITGIL 
      SGINMSGDL KAPSTNIPN GSLAAISTG TFLYLVFVV 
      FLGATCHRN VLLTDFMIA AKVSAIRVL LLAGLYVSS 
      MSSCLGAMY GTPRVLQSI ALENVIPGI SILGMGRGP 
      NKVPLYSMA VVAIVTLTF IMIGEINTL APIVTMPYL 
      LTYACIDYS YFALAQTFD IQHQREQRF DRPKIHQTF 
      SVDSADNDL DRLFPERTR HKTLRGESN SGSPSSPDD 
      TIETTPIAP KTHIHSKPK NWYSNLCNR WFSLFGAAL 
      KIVIMFLVN WVYAIACIS VVFLVWLYI GTANPAVKP 
      GLASEFRFF RWLKTAILR CMGKRVYDY EQIVVTPVH 
      PGLDVTSSQ LNEDNEDFS NRRRYHQSA TVHGHMVAI 
      DD
    CDS sequence
    • >TC001752 RA
      atggatcaa aatacccgc gacaataaa gaccggaac 
      atcgactgg tcccgcttc ggcctggga tcggaaaca 
      gctcaaagg gtccgtgca aacactagt ggttttgtc 
      gacttgtcg aacgattat ggggccggt ggccaccaa 
      gcgtccggt gctaacgaa cttttcgcc gaggagcag 
      ggagacgtc ccttggtgg aaatcaaac tttttcata 
      tcacaacct gttctcttc ggcacttgg gacggtgtt 
      ttcacctca tgtctcatc aacattttc ggggtgatt 
      gtctttctg cggtcgggc tggatcgtg ggccaggct 
      ggcgtgatc agcgcagta ttaatcgtt ttagcgacc 
      gtatttatt gctcttatc tcggttttg tcagccgtg 
      ggcatctgc gagcggtgc cggattgaa agcggtggc 
      gtttatttc gtgatagcg cacgtcctt ggctcgaga 
      tttgggggc tccttgggg ctgctgtac tgcttcgga 
      caggccgta gggtgtgct ttaaatgtg ctaggtttt 
      ggggagtcg atagctgag ttgatcggt tttggtgaa 
      tacaaaatc gcagttagg atcatagca agtgcggcg 
      gttttgttc ttaagtgtg attaacgtt gctggcgtc 
      aaatgggtc ataaagctg cagtttata cttttgtta 
      atactactt ttggccgga ttggatttt atggttggc 
      agctttgtt catacagat gttgagaat ggttttgat 
      ggttggttg ggcggcaac ttgcatgcg aatttgtgg 
      tcggagtac aaggatggt tatagttgg tttaccgtg 
      ttcggggtg ttttttccg acaatcacc gggattttg 
      tccgggatt aacatgagt ggggatttg aaggcgccg 
      tctacgaat atcccgaac ggatcattg gcagctata 
      agcactggg acgtttctt tatttagtt tttgttgta 
      ttcctagga gcgacgtgt catagaaac gttctattg 
      actgatttt atgatcgcc gctaaagtt tccgccatt 
      cgtgtctta ctactggct gggctttat gtctcgtca 
      atgtccagc tgtttgggg gctatgtac gggactccg 
      agagtcttg cagtccata gctttggaa aacgtcatt 
      ccagggatc agtatttta ggaatgggg agaggacca 
      aataaagtt cccctatac tcaatggca gtcgttgca 
      atcgtaaca ctgacattt ataatgata ggggaaata 
      aatacgtta gcaccaatt gtaacaatg ccttattta 
      ctgacatat gcctgtata gactattca tattttgct 
      ttagcccaa acctttgat atacaacat caaagggaa 
      cagcgattc gatcgcccg aaaatccac caaacattc 
      agcgtcgac tcggccgat aacgacctc gaccggctg 
      tttcccgag cgcacaagg cacaaaaca ttgcggggg 
      gagtccaac tcgggttcg cccagcagc cccgacgac 
      acgatcgag acgaccccc atcgcccca aaaacccac 
      atccactca aaaccgaaa aactggtac tccaactta 
      tgcaatcgc tggttttca ctttttggc gccgcttta 
      aaaatcgtc atcatgttt ctcgtaaat tgggtttac 
      gcaattgct tgtataagc gttgtgttt ctcgtatgg 
      ttgtacatc gggaccgct aatccggcc gttaaaccc 
      ggcctagcg tccgaattt cgatttttc agatggctg 
      aaaacggcc attttgcga tgcatgggg aagagagtt 
      tatgactac gaacaaatc gttgttacc cccgtccat 
      ccaggcctt gatgttacg tcatcgcaa ttaaatgaa 
      gataatgaa gatttttcc aatcgccga cgctatcac 
      cagtcggcc actgtgcac ggccacatg gtggccatc 
      gacgactaa
    mRNA sequence
    • >TC001752 RA
      tcatcacaa aaaccatat cacttgtca ggttataaa 
      ttaggagaa agatatgat aaccgagag ataatgtag 
      actttttac aatcgataa tgatcttgt gaggtagaa 
      gtataatgc ggaattcag tttttatcg tcccgataa 
      tgttttgtt catgaaaga aaatggatc aaaataccc 
      gcgacaata aagaccgga acatcgact ggtcccgct 
      tcggcctgg gatcggaaa cagctcaaa gggtccgtg 
      caaacacta gtggttttg tcgacttgt cgaacgatt 
      atggggccg gtggccacc aagcgtccg gtgctaacg 
      aacttttcg ccgaggagc agggagacg tcccttggt 
      ggaaatcaa actttttca tatcacaac ctgttctct 
      tcggcactt gggacggtg ttttcacct catgtctca 
      tcaacattt tcggggtga ttgtctttc tgcggtcgg 
      gctggatcg tgggccagg ctggcgtga tcagcgcag 
      tattaatcg ttttagcga ccgtattta ttgctctta 
      tctcggttt tgtcagccg tgggcatct gcgagcggt 
      gccggattg aaagcggtg gcgtttatt tcgtgatag 
      cgcacgtcc ttggctcga gatttgggg gctccttgg 
      ggctgctgt actgcttcg gacaggccg tagggtgtg 
      ctttaaatg tgctaggtt ttggggagt cgatagctg 
      agttgatcg gttttggtg aatacaaaa tcgcagtta 
      ggatcatag caagtgcgg cggttttgt tcttaagtg 
      tgattaacg ttgctggcg tcaaatggg tcataaagc 
      tgcagttta tacttttgt taatactac ttttggccg 
      gattggatt ttatggttg gcagctttg ttcatacag 
      atgttgaga atggttttg atggttggt tgggcggca 
      acttgcatg cgaatttgt ggtcggagt acaaggatg 
      gttatagtt ggtttaccg tgttcgggg tgttttttc 
      cgacaatca ccgggattt tgtccggga ttaacatga 
      gtggggatt tgaaggcgc cgtctacga atatcccga 
      acggatcat tggcagcta taagcactg ggacgtttc 
      tttatttag tttttgttg tattcctag gagcgacgt 
      gtcatagaa acgttctat tgactgatt ttatgatcg 
      ccgctaaag tttccgcca ttcgtgtct tactactgg 
      ctgggcttt atgtctcgt caatgtcca gctgtttgg 
      gggctatgt acgggactc cgagagtct tgcagtcca 
      tagctttgg aaaacgtca ttccaggga tcagtattt 
      taggaatgg ggagaggac caaataaag ttcccctat 
      actcaatgg cagtcgttg caatcgtaa cactgacat 
      ttataatga taggggaaa taaatacgt tagcaccaa 
      ttgtaacaa tgccttatt tactgacat atgcctgta 
      tagactatt catattttg ctttagccc aaacctttg 
      atatacaac atcaaaggg aacagcgat tcgatcgcc 
      cgaaaatcc accaaacat tcagcgtcg actcggccg 
      ataacgacc tcgaccggc tgtttcccg agcgcacaa 
      ggcacaaaa cattgcggg gggagtcca actcgggtt 
      cgcccagca gccccgacg acacgatcg agacgaccc 
      ccatcgccc caaaaaccc acatccact caaaaccga 
      aaaactggt actccaact tatgcaatc gctggtttt 
      cactttttg gcgccgctt taaaaatcg tcatcatgt 
      ttctcgtaa attgggttt acgcaattg cttgtataa 
      gcgttgtgt ttctcgtat ggttgtaca tcgggaccg 
      ctaatccgg ccgttaaac ccggcctag cgtccgaat 
      ttcgatttt tcagatggc tgaaaacgg ccattttgc 
      gatgcatgg ggaagagag tttatgact acgaacaaa 
      tcgttgtta cccccgtcc atccaggcc ttgatgtta 
      cgtcatcgc aattaaatg aagataatg aagattttt 
      ccaatcgcc gacgctatc accagtcgg ccactgtgc 
      acggccaca tggtggcca tcgacgact aatctgcct 
      cattttaac ttaacaaca ctaattgta tatattttt 
      gtaattatg gacttatca agattaatg ttgtaatta 
      tacccgtta ataatcagt cttttttat ggcgcaaac 
      caactttat tatttattt tcaatataa tttaaacac 
      aaattggcg agtttcatt gaataaaat tgacaacat 
      ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY