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Gene information
General information
TC001751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7984110..7986007
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001751
    iBeetle screen iB_00295

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 6
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 85.71% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001751 RA
      MSEIDEIRA KLRDQKHEH LLRYWPELN NEEKKHLLE 
QLKSIDIEK INDLFRRAE SSYKETQRT YLDDQMKPL 
DPSHFESEE TSTEETLNK YRTFGLEEI AAGRVAVLL 
LAGGQGTRL GVSYPKGMY NVGLPSGKT LFQLQAERI 
RRLQTLAKG KTGKSGKIP WYIMTSGPT DEATEKFLQ 
QNNYFGLDK TDVVLFKQG LLPCFDFEG RAFLDGKAN 
IARAPDGNG GIYRALAQN KILDDMQQR GVKYVHVHS 
VDNILVKVA DPVFVGYCK SKEADCAAK VVQKTNPDE 
AVGVVCFVD GQVRVVEYS EITPSTASL TDKNDRLVF 
NAGNICNHL FTVDFLKEV ADKYEDKLK LHVAKKKIP 
YLDDEGNLV KPAQVSGVK IEKFVFDVF PFSKKFVAW 
EVPRNSEFS AMKNADKDK KDCPSTAKS DLLALHKRY 
IEKAGGVVR CDEVEISPL LSYEGENLE QVRGKVFEK 
PIILAPDEE LTCS
    CDS sequence
    • >TC001751 RA
      atgtcagaa atcgacgaa atcagggcg aaactccgt 
gaccagaag cacgaacac ttgctccgg tattggccc 
gaactcaac aatgaagaa aaaaagcac ctgctcgag 
cagctcaaa tccatcgac atcgaaaaa atcaacgat 
ttgtttcga cgagccgag agctcgtac aaagagacc 
caacggacg tacttggac gaccaaatg aaacccctg 
gacccgtcc cacttcgag tccgaagaa acctccacg 
gaggagacc ctcaacaag taccgaact ttcggactt 
gaggaaatc gcggccgga cgggtggcc gttctcctg 
ctggccggg ggtcaaggg acgcgccta ggggtctcc 
taccccaag ggtatgtac aacgtgggc ctcccctcc 
ggaaaaacc ctcttccag ctccaggcg gagcgcatc 
cgccggctg caaaccctg gcgaagggg aagacggga 
aagtcgggg aaaatcccg tggtacatc atgaccagc 
ggaccgact gacgaagcc acggagaag tttctccaa 
cagaataac tactttggg ctcgacaaa accgacgtt 
gtgttattc aagcaggga cttctccct tgtttcgat 
ttcgaaggc cgcgctttt ctggacggg aaggcgaac 
atcgcccgc gctccggac gggaacggg gggatctac 
cgggcttta gcccagaac aaaatcctg gatgacatg 
cagcagcgc ggggtgaaa tacgtgcat gtgcacagt 
gtcgacaat attttggtc aaagtggcg gaccccgtt 
ttcgtcggt tactgcaag tcgaaggag gcggattgt 
gcggcgaaa gtggtgcaa aaaaccaac ccggacgaa 
gccgtcggg gtggtttgt ttcgtcgac ggccaggtc 
cgggtggtt gaatacagc gaaataacg ccaagtacc 
gcgagtttg accgataag aacgaccgt ttggtcttc 
aatgccggc aatatttgt aaccattta ttcacggtt 
gacttcctc aaagaagtg gcggacaag tacgaagat 
aagcttaaa ctccacgtc gccaaaaag aaaatcccg 
tatttggac gacgaggga aaccttgtc aagccggcc 
caagtgtcg ggggtcaaa atcgagaaa ttcgtgttc 
gacgttttc cccttttcg aaaaagttc gtcgcttgg 
gaagtaccg cgaaatagc gagttcagt gcgatgaaa 
aacgccgat aaagataaa aaagactgt ccctcaacc 
gcaaagagt gacttactc gctctgcac aaaagatac 
atcgagaaa gctggcggg gtcgtgcga tgcgacgaa 
gtggaaatt tctccgctg ttgtcgtac gaaggtgaa 
aatctggag caagtgcgc ggaaaagtg ttcgagaaa 
cccatcatt ttggcaccc gacgaagaa ctcacctgt 
tcgtaa
    mRNA sequence
    • >TC001751 RA
      cgtaaaacg aaaactcaa aaattggta aacgacgct 
caacgtact tttttcttg atttcttat ctcgccaag 
tagaaattc cacattttt tgccttgtt cgaaacagt 
atgcatttg gagctgcta tctaagcta tcgtttatc 
agatcagaa accgctata ggtgatagt ttcctaata 
aaacaatcg agcaccgtt cgaaaagcg agagtaatc 
agtgcggta gtgaacagt cgacggtcg aagaacgtt 
caaggcgac aaatgtttc ctacctttt gaaataaat 
aaataagtg tcagtcaaa tccgcaact cggccgaaa 
tcatgtcag aaatcgacg aaatcaggg cgaaactcc 
gtgaccaga agcacgaac acttgctcc ggtattggc 
ccgaactca acaatgaag aaaaaaagc acctgctcg 
agcagctca aatccatcg acatcgaaa aaatcaacg 
atttgtttc gacgagccg agagctcgt acaaagaga 
cccaacgga cgtacttgg acgaccaaa tgaaacccc 
tggacccgt cccacttcg agtccgaag aaacctcca 
cggaggaga ccctcaaca agtaccgaa ctttcggac 
ttgaggaaa tcgcggccg gacgggtgg ccgttctcc 
tgctggccg ggggtcaag ggacgcgcc taggggtct 
cctacccca agggtatgt acaacgtgg gcctcccct 
ccggaaaaa ccctcttcc agctccagg cggagcgca 
tccgccggc tgcaaaccc tggcgaagg ggaagacgg 
gaaagtcgg ggaaaatcc cgtggtaca tcatgacca 
gcggaccga ctgacgaag ccacggaga agtttctcc 
aacagaata actactttg ggctcgaca aaaccgacg 
ttgtgttat tcaagcagg gacttctcc cttgtttcg 
atttcgaag gccgcgctt ttctggacg ggaaggcga 
acatcgccc gcgctccgg acgggaacg gggggatct 
accgggctt tagcccaga acaaaatcc tggatgaca 
tgcagcagc gcggggtga aatacgtgc atgtgcaca 
gtgtcgaca atattttgg tcaaagtgg cggaccccg 
ttttcgtcg gttactgca agtcgaagg aggcggatt 
gtgcggcga aagtggtgc aaaaaacca acccggacg 
aagccgtcg gggtggttt gtttcgtcg acggccagg 
tccgggtgg ttgaataca gcgaaataa cgccaagta 
ccgcgagtt tgaccgata agaacgacc gtttggtct 
tcaatgccg gcaatattt gtaaccatt tattcacgg 
ttgacttcc tcaaagaag tggcggaca agtacgaag 
ataagctta aactccacg tcgccaaaa agaaaatcc 
cgtatttgg acgacgagg gaaaccttg tcaagccgg 
cccaagtgt cgggggtca aaatcgaga aattcgtgt 
tcgacgttt tcccctttt cgaaaaagt tcgtcgctt 
gggaagtac cgcgaaata gcgagttca gtgcgatga 
aaaacgccg ataaagata aaaaagact gtccctcaa 
ccgcaaaga gtgacttac tcgctctgc acaaaagat 
acatcgaga aagctggcg gggtcgtgc gatgcgacg 
aagtggaaa tttctccgc tgttgtcgt acgaaggtg 
aaaatctgg agcaagtgc gcggaaaag tgttcgaga 
aacccatca ttttggcac ccgacgaag aactcacct 
gttcgtaaa tcagataac cagatattt tatctaaag 
tattgtgct caaaacata tttatttat tgtatttta 
ttgggtgaa aggtactac aaactgtaa aaacatatt 
tttttaaat aaatcattc ttctatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY