General information | |||
TC001751 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7984110..7986007 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001751 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00295 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 85.71% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
- >TC001751 RA
MSEIDEIRA KLRDQKHEH LLRYWPELN NEEKKHLLE QLKSIDIEK INDLFRRAE SSYKETQRT YLDDQMKPL DPSHFESEE TSTEETLNK YRTFGLEEI AAGRVAVLL LAGGQGTRL GVSYPKGMY NVGLPSGKT LFQLQAERI RRLQTLAKG KTGKSGKIP WYIMTSGPT DEATEKFLQ QNNYFGLDK TDVVLFKQG LLPCFDFEG RAFLDGKAN IARAPDGNG GIYRALAQN KILDDMQQR GVKYVHVHS VDNILVKVA DPVFVGYCK SKEADCAAK VVQKTNPDE AVGVVCFVD GQVRVVEYS EITPSTASL TDKNDRLVF NAGNICNHL FTVDFLKEV ADKYEDKLK LHVAKKKIP YLDDEGNLV KPAQVSGVK IEKFVFDVF PFSKKFVAW EVPRNSEFS AMKNADKDK KDCPSTAKS DLLALHKRY IEKAGGVVR CDEVEISPL LSYEGENLE QVRGKVFEK PIILAPDEE LTCS
- >TC001751 RA
atgtcagaa atcgacgaa atcagggcg aaactccgt gaccagaag cacgaacac ttgctccgg tattggccc gaactcaac aatgaagaa aaaaagcac ctgctcgag cagctcaaa tccatcgac atcgaaaaa atcaacgat ttgtttcga cgagccgag agctcgtac aaagagacc caacggacg tacttggac gaccaaatg aaacccctg gacccgtcc cacttcgag tccgaagaa acctccacg gaggagacc ctcaacaag taccgaact ttcggactt gaggaaatc gcggccgga cgggtggcc gttctcctg ctggccggg ggtcaaggg acgcgccta ggggtctcc taccccaag ggtatgtac aacgtgggc ctcccctcc ggaaaaacc ctcttccag ctccaggcg gagcgcatc cgccggctg caaaccctg gcgaagggg aagacggga aagtcgggg aaaatcccg tggtacatc atgaccagc ggaccgact gacgaagcc acggagaag tttctccaa cagaataac tactttggg ctcgacaaa accgacgtt gtgttattc aagcaggga cttctccct tgtttcgat ttcgaaggc cgcgctttt ctggacggg aaggcgaac atcgcccgc gctccggac gggaacggg gggatctac cgggcttta gcccagaac aaaatcctg gatgacatg cagcagcgc ggggtgaaa tacgtgcat gtgcacagt gtcgacaat attttggtc aaagtggcg gaccccgtt ttcgtcggt tactgcaag tcgaaggag gcggattgt gcggcgaaa gtggtgcaa aaaaccaac ccggacgaa gccgtcggg gtggtttgt ttcgtcgac ggccaggtc cgggtggtt gaatacagc gaaataacg ccaagtacc gcgagtttg accgataag aacgaccgt ttggtcttc aatgccggc aatatttgt aaccattta ttcacggtt gacttcctc aaagaagtg gcggacaag tacgaagat aagcttaaa ctccacgtc gccaaaaag aaaatcccg tatttggac gacgaggga aaccttgtc aagccggcc caagtgtcg ggggtcaaa atcgagaaa ttcgtgttc gacgttttc cccttttcg aaaaagttc gtcgcttgg gaagtaccg cgaaatagc gagttcagt gcgatgaaa aacgccgat aaagataaa aaagactgt ccctcaacc gcaaagagt gacttactc gctctgcac aaaagatac atcgagaaa gctggcggg gtcgtgcga tgcgacgaa gtggaaatt tctccgctg ttgtcgtac gaaggtgaa aatctggag caagtgcgc ggaaaagtg ttcgagaaa cccatcatt ttggcaccc gacgaagaa ctcacctgt tcgtaa
- >TC001751 RA
cgtaaaacg aaaactcaa aaattggta aacgacgct caacgtact tttttcttg atttcttat ctcgccaag tagaaattc cacattttt tgccttgtt cgaaacagt atgcatttg gagctgcta tctaagcta tcgtttatc agatcagaa accgctata ggtgatagt ttcctaata aaacaatcg agcaccgtt cgaaaagcg agagtaatc agtgcggta gtgaacagt cgacggtcg aagaacgtt caaggcgac aaatgtttc ctacctttt gaaataaat aaataagtg tcagtcaaa tccgcaact cggccgaaa tcatgtcag aaatcgacg aaatcaggg cgaaactcc gtgaccaga agcacgaac acttgctcc ggtattggc ccgaactca acaatgaag aaaaaaagc acctgctcg agcagctca aatccatcg acatcgaaa aaatcaacg atttgtttc gacgagccg agagctcgt acaaagaga cccaacgga cgtacttgg acgaccaaa tgaaacccc tggacccgt cccacttcg agtccgaag aaacctcca cggaggaga ccctcaaca agtaccgaa ctttcggac ttgaggaaa tcgcggccg gacgggtgg ccgttctcc tgctggccg ggggtcaag ggacgcgcc taggggtct cctacccca agggtatgt acaacgtgg gcctcccct ccggaaaaa ccctcttcc agctccagg cggagcgca tccgccggc tgcaaaccc tggcgaagg ggaagacgg gaaagtcgg ggaaaatcc cgtggtaca tcatgacca gcggaccga ctgacgaag ccacggaga agtttctcc aacagaata actactttg ggctcgaca aaaccgacg ttgtgttat tcaagcagg gacttctcc cttgtttcg atttcgaag gccgcgctt ttctggacg ggaaggcga acatcgccc gcgctccgg acgggaacg gggggatct accgggctt tagcccaga acaaaatcc tggatgaca tgcagcagc gcggggtga aatacgtgc atgtgcaca gtgtcgaca atattttgg tcaaagtgg cggaccccg ttttcgtcg gttactgca agtcgaagg aggcggatt gtgcggcga aagtggtgc aaaaaacca acccggacg aagccgtcg gggtggttt gtttcgtcg acggccagg tccgggtgg ttgaataca gcgaaataa cgccaagta ccgcgagtt tgaccgata agaacgacc gtttggtct tcaatgccg gcaatattt gtaaccatt tattcacgg ttgacttcc tcaaagaag tggcggaca agtacgaag ataagctta aactccacg tcgccaaaa agaaaatcc cgtatttgg acgacgagg gaaaccttg tcaagccgg cccaagtgt cgggggtca aaatcgaga aattcgtgt tcgacgttt tcccctttt cgaaaaagt tcgtcgctt gggaagtac cgcgaaata gcgagttca gtgcgatga aaaacgccg ataaagata aaaaagact gtccctcaa ccgcaaaga gtgacttac tcgctctgc acaaaagat acatcgaga aagctggcg gggtcgtgc gatgcgacg aagtggaaa tttctccgc tgttgtcgt acgaaggtg aaaatctgg agcaagtgc gcggaaaag tgttcgaga aacccatca ttttggcac ccgacgaag aactcacct gttcgtaaa tcagataac cagatattt tatctaaag tattgtgct caaaacata tttatttat tgtatttta ttgggtgaa aggtactac aaactgtaa aaacatatt tttttaaat aaatcattc ttctatcc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
