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Gene information
General information
TC001744 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8121559..8130345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001744
    iBeetle screen iB_00293

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001744 RA
      MASRKNVLN LLQLVTSNS CKCPAHSNV GLVHSSTVP 
      NALPSKEYA FEMSSSTVR YGPGVTQEV GMDIVNMRA 
      KNVCVVTDP KLAKLPPVQ ATLDSLTKN NVQFQVYDQ 
      VRVEPTEES LLHAANFAK SSNFDAFIA VGGGSVIDT 
      AKAANLYAC NPDAEFLDY VNAPIGKAK PIEKPLKPL 
      IAIPTTSGT GSETTGVSI FDYKPLHAK TGISHKALR 
      PTLGLIDPL HTLSLPEKV AAYSGFDVF CHALESFTA 
      IPYNERKPC PSNPALRPT YQGQNPISD VWARYALNI 
      IKKYFKRAV YQPDDLEAR SAMHLASTM AGVGFGNAG 
      VHLCHGLSY PISGNVRTF KPKDYDDDH AIIPHGLSV 
      VMSAPAVFQ FTAPACPER HLEAAEVLG TDVRNAKKA 
      DAGKILSET MREYMNVIK IENGLSALG FVKDDIPQL 
      VKGTLPQNR ITKMAPREQ SEEDLAHLF EQSMTVY
    CDS sequence
    • >TC001744 RA
      atggcatcc cgtaagaac gtcttaaac ctcctccag 
      ctcgtaaca tctaattcg tgtaaatgt ccggcccat 
      tccaatgtt ggattagtg cattccagc actgtacct 
      aatgcccta ccctccaag gaatacgct ttcgaaatg 
      agttcctcc accgttcgt tacggccca ggtgtgacc 
      caagaagtt ggcatggac atagtcaac atgagagcc 
      aaaaacgtc tgcgttgta accgacccc aaactcgca 
      aaactgcct ccagtgcaa gccactctc gactcgcta 
      acaaaaaac aacgtccag ttccaagtc tacgaccaa 
      gtcagagtc gaaccaacc gaagaaagc ctcctccat 
      gcagctaac tttgcaaaa agcagcaat tttgatgcg 
      tttattgct gtaggtggg ggctcagtc atcgacaca 
      gccaaagca gccaactta tatgcgtgt aacccggat 
      gccgagttc ttggactat gttaacgca cccattggg 
      aaagccaaa ccgatcgaa aagccgctg aaaccacta 
      attgccatt ccaaccact tcagggacc gggagcgag 
      accaccggg gttagcatt ttcgactac aagccgctc 
      cacgcaaaa accggaatt tcccacaag gctttaagg 
      cccactttg ggtctaatt gatccgctt cacacgtta 
      tcgcttccg gaaaaagtg gcggcatac agcggcttt 
      gacgtgttc tgtcatgct ttggagtct ttcactgct 
      attccctac aatgagagg aaaccttgt ccgtcaaac 
      ccggcctta aggccgact tatcaaggc cagaatccc 
      atatcagat gtatgggcg cgctacgcc cttaatatt 
      atcaagaaa tatttcaaa agggctgtg tatcaaccc 
      gacgatctt gaggcaagg tctgcgatg catttagcg 
      tccactatg gcaggtgtt gggtttggc aacgcaggg 
      gtgcacctt tgtcacggg ttgtcgtat cccatatct 
      gggaacgtc cggactttc aaacccaag gattacgat 
      gacgaccat gctatcatt cctcatggg ttgagtgtg 
      gtcatgagt gcacccgct gtgttccaa tttaccgcg 
      cctgcgtgc ccagagaga catttggag gcagctgaa 
      gtgcttggg accgacgtt cgcaatgcc aaaaaagcc 
      gatgctggg aaaatcctt tctgaaact atgagagaa 
      tacatgaat gtgatcaag attgaaaat ggtttgtcg 
      gctctagga ttcgtcaaa gatgatatc ccccaacta 
      gtcaaagga actcttcca cagaatcgc attaccaag 
      atggcacca cgggaacaa tcagaagaa gacttggcg 
      catttgttt gaacaatca atgactgta tactga
    mRNA sequence
    • >TC001744 RA
      atcaattta aaaacaatt ttcaaatta gtcgaatat 
      tttttaaaa caaaaagat ccgtatatt ttgaaagtg 
      agggttcag aaatgattt aagtaagaa ttggagaaa 
      cctttggac ttgtttatc aaccacaaa cgaaagcga 
      ttcctgttc atagttgtc tgatccgcg aaaggttca 
      gaatcgtta aagatggca tcccgtaag aacgtctta 
      aacctcctc cagctcgta acatctaat tcgtgtaaa 
      tgtccggcc cattccaat gttggatta gtgcattcc 
      agcactgta cctaatgcc ctaccctcc aaggaatac 
      gctttcgaa atgagttcc tccaccgtt cgttacggc 
      ccaggtgtg acccaagaa gttggcatg gacatagtc 
      aacatgaga gccaaaaac gtctgcgtt gtaaccgac 
      cccaaactc gcaaaactg cctccagtg caagccact 
      ctcgactcg ctaacaaaa aacaacgtc cagttccaa 
      gtctacgac caagtcaga gtcgaacca accgaagaa 
      agcctcctc catgcagct aactttgca aaaagcagc 
      aattttgat gcgtttatt gctgtaggt gggggctca 
      gtcatcgac acagccaaa gcagccaac ttatatgcg 
      tgtaacccg gatgccgag ttcttggac tatgttaac 
      gcacccatt gggaaagcc aaaccgatc gaaaagccg 
      ctgaaacca ctaattgcc attccaacc acttcaggg 
      accgggagc gagaccacc ggggttagc attttcgac 
      tacaagccg ctccacgca aaaaccgga atttcccac 
      aaggcttta aggcccact ttgggtcta attgatccg 
      cttcacacg ttatcgctt ccggaaaaa gtggcggca 
      tacagcggc tttgacgtg ttctgtcat gctttggag 
      tctttcact gctattccc tacaatgag aggaaacct 
      tgtccgtca aacccggcc ttaaggccg acttatcaa 
      ggccagaat cccatatca gatgtatgg gcgcgctac 
      gcccttaat attatcaag aaatatttc aaaagggct 
      gtgtatcaa cccgacgat cttgaggca aggtctgcg 
      atgcattta gcgtccact atggcaggt gttgggttt 
      ggcaacgca ggggtgcac ctttgtcac gggttgtcg 
      tatcccata tctgggaac gtccggact ttcaaaccc 
      aaggattac gatgacgac catgctatc attcctcat 
      gggttgagt gtggtcatg agtgcaccc gctgtgttc 
      caatttacc gcgcctgcg tgcccagag agacatttg 
      gaggcagct gaagtgctt gggaccgac gttcgcaat 
      gccaaaaaa gccgatgct gggaaaatc ctttctgaa 
      actatgaga gaatacatg aatgtgatc aagattgaa 
      aatggtttg tcggctcta ggattcgtc aaagatgat 
      atcccccaa ctagtcaaa ggaactctt ccacagaat 
      cgcattacc aagatggca ccacgggaa caatcagaa 
      gaagacttg gcgcatttg tttgaacaa tcaatgact 
      gtatactga gtatttaat aaaactcga cgtatctac 
      aatacattc tggctaatt atattagtt acaggaaat 
      atattacgt acatcaaaa gccattaaa tcggtttaa 
      tagcatgtt tgtgcccaa acaatactg taaataaat 
      ttcgatatg ttcctaata agtgataca aataaatca 
      ttccagatt attgtgcga aataaatat ttcatttac 
      ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY