Status
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Gene information
General information
TC001742 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8156137..8160954
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001742
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    follicular epithelium surface irregular - Number of animals showing the phenotype: 4

    Comment: potentially detached from basal membrane
    vitellogenic egg chamber potentially latedegenerated - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late rounded - Number of animals showing the phenotype: 4
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentially fused - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001742 RA
      MTLKGAVFC VIFFTSVTG NFLSCPKLC ICPPGFTDC 
      KYNNFSNLP LGLDKNVTI LDLSYNAFQ VFPDDLNNY 
      PSLEYLNLS YNKIHRLQN DNFRRLTKL ERLDLSHNS 
      INWANIGPY TFSSTLNLL FLDLSYNPL GSQHVGENP 
      LQIQSLEIL RLNNCSIDH LDNKLLDKL PKLKELHLS 
      ENPITSLFW SLASTSLKF LDLSRCNLK HVSPRVFVN 
      LTSLQTLYM QQNINLKSF YCNSPSLRY LDISNCNLE 
      SVPRGYLPN INAANLHGN HFRIIPNKS FANYTNLVM 
      LDLSYNALH VIDENAFVG LDKIERLDL SQNTLSDLS 
      PNVFFSNTN LQFLNLSRN YLNTVPNLN SDFLEILDL 
      SFCEIASVD QDCLVNMPR LKILNLSKN IISVLPDNF 
      RGDALRVLD VSLCRIKTV NNKTFALMS SLRSVNLAG 
      NRLTYASPS SFAQVVSLN ISDNPWRCD CSKLKDLYD 
      WLSKTGKKL DNLYCESPE AFEGRSWLS ACQKDWQPD 
      ITRSDSVWW YTTGLIITV FVILFVILA MRRMYLLKE 
      KRLREANEA RRNEEREAL RRMHRMQAE AREEASRNA 
      PDPRELQTP PSYNEALLL PRLDSSHPS LSASLHSIA 
      SKHSINGSL TDVAKRRRK RRRRKSDSS EGQRASRVV 
      VVDSDTSEH EPEPRTTLE SDF
    CDS sequence
    • >TC001742 RA
      atgacgctg aagggtgca gtgttctgt gtcatattt 
      ttcacatca gtaaccggc aattttttg tcatgtcca 
      aaattatgt atatgccca cctggtttc acggactgc 
      aaatataac aatttttcg aatttaccc ctcggactt 
      gacaaaaat gtgaccatt ctggacctg tcctacaac 
      gcgtttcaa gtatttccc gacgattta aacaattat 
      ccatctctg gagtactta aacctttcg tataacaaa 
      atccaccga ctccagaat gacaatttt cgaagatta 
      acgaaacta gaacgattg gacttgagc cacaattcg 
      atcaactgg gcaaacatc ggaccatac acattttcg 
      tcgactctc aacttactg ttcctggat ttgtcatac 
      aacccgctt ggaagtcaa catgtgggc gaaaaccca 
      ctacaaatc cagtcgttg gaaatccta cgcttaaac 
      aactgctca atagatcac ctcgataat aagctcctc 
      gataaatta ccaaaactc aaggaacta cacctttcc 
      gaaaacccg atcacgtcg ttattttgg agtttggca 
      tcaactagt ttaaaattc ttggatttg agccgatgt 
      aacttgaaa catgtgagt ccgagagtt ttcgtaaac 
      ctaacttcg ttgcaaact ctctacatg cagcaaaat 
      attaatttg aagagtttt tactgcaac tcgcccagt 
      ttgcgctat ttggacatt tcgaactgt aatctggag 
      tcggttccg cgtggttac ctccccaat ataaacgcg 
      gccaatttg cacggtaac cactttaga atcattcca 
      aacaaaagc tttgccaat tatactaat cttgtgatg 
      ttggacttg tcgtataat gctctccat gttattgac 
      gaaaacgcc ttcgttgga cttgacaaa atcgaacgg 
      ttagacttg tcccaaaac accttatcg gacctaagc 
      cccaacgta ttcttcagt aatactaac ttacagttc 
      ttgaatttg tcaaggaat tacttgaat acggtacca 
      aatctgaac agtgacttt ttggagatt ttagatcta 
      agtttctgt gagatagct tcggttgat caggactgt 
      ttggttaat atgccacga ttgaaaatt ttgaattta 
      tccaaaaat atcatttcg gtccttccg gacaatttc 
      cgaggtgat gccttacgg gttctggac gttagtctt 
      tgtaggatt aaaaccgtc aataataaa acctttgca 
      ttgatgtca tcgctccgt tctgtgaat ttggctgga 
      aatcgtttg acttatgcc agcccttcg agtttcgcc 
      caagtggtt tcgttgaac atttcggac aatccctgg 
      cgttgcgat tgctcaaaa ctcaaagat ttgtacgac 
      tggttgtca aaaacgggc aagaagctg gacaacttg 
      tactgtgaa tcgcctgaa gctttcgaa ggtcgttcg 
      tggttgtcc gcatgtcag aaagactgg cagcctgac 
      ataacccga agcgattcc gtttggtgg tacacaacc 
      gggttgata ataaccgtc ttcgtgatt ttattcgtc 
      attctcgcc atgaggaga atgtactta ttaaaagag 
      aaacgcctt cgcgaggcg aacgaagca aggagaaac 
      gaagaacgc gaagctctt cgccgaatg catcgcatg 
      caggcggaa gctcgtgaa gaagcaagc agaaacgca 
      ccagatcct cgagagttg caaacacct ccgtcctac 
      aacgaagcc ttgctctta ccaagacta gactcctcg 
      catccgagt ttatcggcc agcttgcac agcattgcc 
      tccaaacat agcattaac ggcagtcta acggacgtg 
      gcgaaaaga aggcgaaag cggagaaga aggaaatcg 
      gattcttcc gaaggtcaa cgtgcgtct cgggtcgtc 
      gtcgttgac tcggacacc tcagaacac gaacctgaa 
      cctcgcaca acgttagaa agtgatttt taa
    mRNA sequence
    • >TC001742 RA
      actaaaaac ctagccagt aaccgtacc aaaatggtg 
      tagtgttaa attgtttca cgttataat aaaaataat 
      gtatataaa tgattaata ccaaagctc agtaatgac 
      gctgaaggg tgcagtgtt ctgtgtcat atttttcac 
      atcagtaac cggcaattt tttgtcatg tccaaaatt 
      atgtatatg cccacctgg tttcacgga ctgcaaata 
      taacaattt ttcgaattt acccctcgg acttgacaa 
      aaatgtgac cattctgga cctgtccta caacgcgtt 
      tcaagtatt tcccgacga tttaaacaa ttatccatc 
      tctggagta cttaaacct ttcgtataa caaaatcca 
      ccgactcca gaatgacaa ttttcgaag attaacgaa 
      actagaacg attggactt gagccacaa ttcgatcaa 
      ctgggcaaa catcggacc atacacatt ttcgtcgac 
      tctcaactt actgttcct ggatttgtc atacaaccc 
      gcttggaag tcaacatgt gggcgaaaa cccactaca 
      aatccagtc gttggaaat cctacgctt aaacaactg 
      ctcaataga tcacctcga taataagct cctcgataa 
      attaccaaa actcaagga actacacct ttccgaaaa 
      cccgatcac gtcgttatt ttggagttt ggcatcaac 
      tagtttaaa attcttgga tttgagccg atgtaactt 
      gaaacatgt gagtccgag agttttcgt aaacctaac 
      ttcgttgca aactctcta catgcagca aaatattaa 
      tttgaagag tttttactg caactcgcc cagtttgcg 
      ctatttgga catttcgaa ctgtaatct ggagtcggt 
      tccgcgtgg ttacctccc caatataaa cgcggccaa 
      tttgcacgg taaccactt tagaatcat tccaaacaa 
      aagctttgc caattatac taatcttgt gatgttgga 
      cttgtcgta taatgctct ccatgttat tgacgaaaa 
      cgccttcgt tggacttga caaaatcga acggttaga 
      cttgtccca aaacacctt atcggacct aagccccaa 
      cgtattctt cagtaatac taacttaca gttcttgaa 
      tttgtcaag gaattactt gaatacggt accaaatct 
      gaacagtga ctttttgga gattttaga tctaagttt 
      ctgtgagat agcttcggt tgatcagga ctgtttggt 
      taatatgcc acgattgaa aattttgaa tttatccaa 
      aaatatcat ttcggtcct tccggacaa tttccgagg 
      tgatgcctt acgggttct ggacgttag tctttgtag 
      gattaaaac cgtcaataa taaaacctt tgcattgat 
      gtcatcgct ccgttctgt gaatttggc tggaaatcg 
      tttgactta tgccagccc ttcgagttt cgcccaagt 
      ggtttcgtt gaacatttc ggacaatcc ctggcgttg 
      cgattgctc aaaactcaa agatttgta cgactggtt 
      gtcaaaaac gggcaagaa gctggacaa cttgtactg 
      tgaatcgcc tgaagcttt cgaaggtcg ttcgtggtt 
      gtccgcatg tcagaaaga ctggcagcc tgacataac 
      ccgaagcga ttccgtttg gtggtacac aaccgggtt 
      gataataac cgtcttcgt gattttatt cgtcattct 
      cgccatgag gagaatgta cttattaaa agagaaacg 
      ccttcgcga ggcgaacga agcaaggag aaacgaaga 
      acgcgaagc tcttcgccg aatgcatcg catgcaggc 
      ggaagctcg tgaagaagc aagcagaaa cgcaccaga 
      tcctcgaga gttgcaaac acctccgtc ctacaacga 
      agccttgct cttaccaag actagactc ctcgcatcc 
      gagtttatc ggccagctt gcacagcat tgcctccaa 
      acatagcat taacggcag tctaacgga cgtggcgaa 
      aagaaggcg aaagcggag aagaaggaa atcggattc 
      ttccgaagg tcaacgtgc gtctcgggt cgtcgtcgt 
      tgactcgga cacctcaga acacgaacc tgaacctcg 
      cacaacgtt agaaagtga tttttaaac accgattat 
      ggaatcgat ttggtgatt tttttgctg acaggcccg 
      gactagtcg gaactgcat aggagacga ttgtggcga 
      tttttagtc cagtgatat tcccaaaag agatgcaaa 
      ctgtagtaa cactaagca ggattttga taatgttgt 
      ggtgtgact caagttgta ataaatata actaggtta 
      acagtttta tttattctc caccccagc tcaagcgaa 
      ttgaaaaaa atatttggc caatttgct aatgacagc 
      tcactaaat actttaaaa cagtaggaa taatgaagg 
      acttaattt ttgccgcca cgtcggacc aaaactagg 
      tataggcga agaagaact ataaaaata ttaaaataa 
      ctaaatgtt attctagtt ccttttgca actatgtgt 
      tcgtcactt ttccttata aacgagaaa cccaataaa 
      aacactcaa tggct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY