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Gene information
General information
TC034534 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8486167..8496961
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034534
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber early potentiallydegenerated - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection



    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034534 RA
      MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS 
      GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY 
      GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR 
      NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ 
      VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN 
      KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK 
      LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV 
      CVSTPEELI IPDQDISEE TLINSLKDE ASPVGDVNV 
      DNNGSFDNQ KVFLNPSTQ DTLLSNLIK NNTTKDGAK 
      NGKPINAAT SLSSAPVTS NNMAIATRD TNLCDVIKS 
      MPGPSDTYT MDLNNHVEA TQQELDQFK DILNGYNPF 
      VDANALLGE LNKIPQNFK DVYLFNGDS PVYDLNVND 
      DNKNENLID PSDPLVSGS ELTPYGGNF DFTELFDDT 
      INEGNVQTT EATSSTVNT PQIIKEEPI FPAIQNKK
    • >TC034534 RB
      MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS 
      GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY 
      GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR 
      NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ 
      VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN 
      KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK 
      LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV 
      CVSTPEELI IPDQDISEE TLINSLKDE ASPVGDVNV 
      DNNGSFDNQ KVFLNPSTQ DTLLSNLIK NNTTKDGAK 
      NGKPINAAT SLSSAPVTS NNMAIATRD TNLCDVIKS 
      MPGPSDTYT MDLNNHVEA TQQELDQFK DILNGYNPF 
      VDANALLGL FNGDSPVYD LNVNDDNKN ENLIDPSDP 
      LVSGSELTP YGGNFDFTE LFDDTINEG NVQTTEATS 
      STVNTPQII KEEPIFPAI QNKK
    • >TC034534 RC
      MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS 
      GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY 
      GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR 
      NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ 
      VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN 
      KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK 
      LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV 
      VEGSNIEIE NDLCVSTPE ELIIPDQDI SEETLINSL 
      KDEASPVGD VNVDNNGSF DNQKVFLNP STQDTLLSN 
      LIKNNTTKD GAKNGKPIN AATSLSSAP VTSNNMAIA 
      TRDTNLCDV IKSMPGPSD TYTMDLNNH VEATQQELD 
      QFKDILNGY NPFVDANAL LGELNKIPQ NFKDVYLFN 
      GDSPVYDLN VNDDNKNEN LIDPSDPLV SGSELTPYG 
      GNFDFTELF DDTINEGNV QTTEATSST VNTPQIIKE 
      EPIFPAIQN KK
    CDS sequence
    • >TC034534 RA
      atgcaaccg ttaggggaa aatgctgca aacactcca 
      gccttttta ggtaaatta tggaaaatg gttaatgac 
      ccatcgacc gaccacctg atctgctgg agtccgtcc 
      ggcactagc tttgtgata ccaaatcaa gctcaattt 
      tggtatgaa cttcttcct ctctattac aaacacaat 
      aacatgagt agtttcgtg aggcagtta aacatgtat 
      ggtttccat aaaatgtcg acggttgaa aatggtaca 
      atggactca gataaagat gaaattcaa ttttcacat 
      ccgtatttt cttaaagac cagcctgaa ctcttgcgc 
      aatattaaa agaaaagca accacttct aaaacttct 
      aacgaaaat aacaataaa catgacgag ttaactaaa 
      gttctaagc gatgttaaa caactcaga ggacgtcaa 
      gtcagtgtt gataatcaa ctaaatgca atgaaacaa 
      gaaaatgcg cttttgtgg cgcgaagtg gctattctt 
      aggcagaaa catttgaaa caacaaaaa atcgtcaat 
      aagctcatt caattctta gttacgtta gttcaaccg 
      tctagaatg acagttggt gtaaaaagg gggtaccaa 
      ttgatgttg cacgaaagt ccaaataag aaaagcaaa 
      cttagtaat aacggaaaa agtaacatt aatgcagag 
      ggacccaca attcatgaa ttggacagc gaactggat 
      ttaacacca aatttatac gaagaggaa gtaccagtt 
      tgtgtcagt acaccagaa gaattaatt attccggat 
      caggatata agcgaagaa actttgatt aatagttta 
      aaagatgaa gcatcacca gttggtgat gttaatgtt 
      gacaataac ggcagtttt gataatcaa aaagtgttt 
      ttgaatccg tcaactcag gacacgttg ttaagtaat 
      ttaataaaa aataatacg actaaagat ggggctaag 
      aatggaaaa cctataaac gcagccact tcactgtca 
      tctgctcct gttactagt aacaatatg gccattgcc 
      actcgtgat acgaatttg tgtgatgta attaagagt 
      atgccagga cctagcgat acttatacc atggattta 
      aataaccac gtggaagcc actcaacag gaattagac 
      caattcaaa gatatttta aatggatat aatccattt 
      gttgatgct aatgcctta ctaggggaa ttaaacaag 
      atccctcaa aacttcaaa gatgtctat ctctttaat 
      ggcgattct cccgtttat gatctgaat gttaatgat 
      gataataaa aatgaaaac ttgatcgat ccttctgat 
      ccgttggtt tctgggtct gaattaacc ccatatggt 
      ggtaatttt gattttact gaactattt gatgatacc 
      attaatgaa gggaatgta cagacaact gaagctaca 
      tcttcaact gttaataca ccgcaaatt attaaagaa 
      gaacctatt tttccggct atacaaaac aaaaagtaa
    • >TC034534 RB
      atgcaaccg ttaggggaa aatgctgca aacactcca 
      gccttttta ggtaaatta tggaaaatg gttaatgac 
      ccatcgacc gaccacctg atctgctgg agtccgtcc 
      ggcactagc tttgtgata ccaaatcaa gctcaattt 
      tggtatgaa cttcttcct ctctattac aaacacaat 
      aacatgagt agtttcgtg aggcagtta aacatgtat 
      ggtttccat aaaatgtcg acggttgaa aatggtaca 
      atggactca gataaagat gaaattcaa ttttcacat 
      ccgtatttt cttaaagac cagcctgaa ctcttgcgc 
      aatattaaa agaaaagca accacttct aaaacttct 
      aacgaaaat aacaataaa catgacgag ttaactaaa 
      gttctaagc gatgttaaa caactcaga ggacgtcaa 
      gtcagtgtt gataatcaa ctaaatgca atgaaacaa 
      gaaaatgcg cttttgtgg cgcgaagtg gctattctt 
      aggcagaaa catttgaaa caacaaaaa atcgtcaat 
      aagctcatt caattctta gttacgtta gttcaaccg 
      tctagaatg acagttggt gtaaaaagg gggtaccaa 
      ttgatgttg cacgaaagt ccaaataag aaaagcaaa 
      cttagtaat aacggaaaa agtaacatt aatgcagag 
      ggacccaca attcatgaa ttggacagc gaactggat 
      ttaacacca aatttatac gaagaggaa gtaccagtt 
      tgtgtcagt acaccagaa gaattaatt attccggat 
      caggatata agcgaagaa actttgatt aatagttta 
      aaagatgaa gcatcacca gttggtgat gttaatgtt 
      gacaataac ggcagtttt gataatcaa aaagtgttt 
      ttgaatccg tcaactcag gacacgttg ttaagtaat 
      ttaataaaa aataatacg actaaagat ggggctaag 
      aatggaaaa cctataaac gcagccact tcactgtca 
      tctgctcct gttactagt aacaatatg gccattgcc 
      actcgtgat acgaatttg tgtgatgta attaagagt 
      atgccagga cctagcgat acttatacc atggattta 
      aataaccac gtggaagcc actcaacag gaattagac 
      caattcaaa gatatttta aatggatat aatccattt 
      gttgatgct aatgcctta ctagggctc tttaatggc 
      gattctccc gtttatgat ctgaatgtt aatgatgat 
      aataaaaat gaaaacttg atcgatcct tctgatccg 
      ttggtttct gggtctgaa ttaacccca tatggtggt 
      aattttgat tttactgaa ctatttgat gataccatt 
      aatgaaggg aatgtacag acaactgaa gctacatct 
      tcaactgtt aatacaccg caaattatt aaagaagaa 
      cctattttt ccggctata caaaacaaa aagtaa
    • >TC034534 RC
      atgcaaccg ttaggggaa aatgctgca aacactcca 
      gccttttta ggtaaatta tggaaaatg gttaatgac 
      ccatcgacc gaccacctg atctgctgg agtccgtcc 
      ggcactagc tttgtgata ccaaatcaa gctcaattt 
      tggtatgaa cttcttcct ctctattac aaacacaat 
      aacatgagt agtttcgtg aggcagtta aacatgtat 
      ggtttccat aaaatgtcg acggttgaa aatggtaca 
      atggactca gataaagat gaaattcaa ttttcacat 
      ccgtatttt cttaaagac cagcctgaa ctcttgcgc 
      aatattaaa agaaaagca accacttct aaaacttct 
      aacgaaaat aacaataaa catgacgag ttaactaaa 
      gttctaagc gatgttaaa caactcaga ggacgtcaa 
      gtcagtgtt gataatcaa ctaaatgca atgaaacaa 
      gaaaatgcg cttttgtgg cgcgaagtg gctattctt 
      aggcagaaa catttgaaa caacaaaaa atcgtcaat 
      aagctcatt caattctta gttacgtta gttcaaccg 
      tctagaatg acagttggt gtaaaaagg gggtaccaa 
      ttgatgttg cacgaaagt ccaaataag aaaagcaaa 
      cttagtaat aacggaaaa agtaacatt aatgcagag 
      ggacccaca attcatgaa ttggacagc gaactggat 
      ttaacacca aatttatac gaagaggaa gtaccagtt 
      gtggaagga agcaacatc gaaattgaa aatgacttg 
      tgtgtcagt acaccagaa gaattaatt attccggat 
      caggatata agcgaagaa actttgatt aatagttta 
      aaagatgaa gcatcacca gttggtgat gttaatgtt 
      gacaataac ggcagtttt gataatcaa aaagtgttt 
      ttgaatccg tcaactcag gacacgttg ttaagtaat 
      ttaataaaa aataatacg actaaagat ggggctaag 
      aatggaaaa cctataaac gcagccact tcactgtca 
      tctgctcct gttactagt aacaatatg gccattgcc 
      actcgtgat acgaatttg tgtgatgta attaagagt 
      atgccagga cctagcgat acttatacc atggattta 
      aataaccac gtggaagcc actcaacag gaattagac 
      caattcaaa gatatttta aatggatat aatccattt 
      gttgatgct aatgcctta ctaggggaa ttaaacaag 
      atccctcaa aacttcaaa gatgtctat ctctttaat 
      ggcgattct cccgtttat gatctgaat gttaatgat 
      gataataaa aatgaaaac ttgatcgat ccttctgat 
      ccgttggtt tctgggtct gaattaacc ccatatggt 
      ggtaatttt gattttact gaactattt gatgatacc 
      attaatgaa gggaatgta cagacaact gaagctaca 
      tcttcaact gttaataca ccgcaaatt attaaagaa 
      gaacctatt tttccggct atacaaaac aaaaagtaa
    mRNA sequence
    • >TC034534 RA
      gtagtgcca ccactcacc atctgtttg tttaatgtt 
      caggtcaat aaacacacg ttagggagt attgtacaa 
      taagaaaag gtcctacaa tgcaaccgt taggggaaa 
      atgctgcaa acactccag cctttttag gtaaattat 
      ggaaaatgg ttaatgacc catcgaccg accacctga 
      tctgctgga gtccgtccg gcactagct ttgtgatac 
      caaatcaag ctcaatttt ggtatgaac ttcttcctc 
      tctattaca aacacaata acatgagta gtttcgtga 
      ggcagttaa acatgtatg gtttccata aaatgtcga 
      cggttgaaa atggtacaa tggactcag ataaagatg 
      aaattcaat tttcacatc cgtattttc ttaaagacc 
      agcctgaac tcttgcgca atattaaaa gaaaagcaa 
      ccacttcta aaacttcta acgaaaata acaataaac 
      atgacgagt taactaaag ttctaagcg atgttaaac 
      aactcagag gacgtcaag tcagtgttg ataatcaac 
      taaatgcaa tgaaacaag aaaatgcgc ttttgtggc 
      gcgaagtgg ctattctta ggcagaaac atttgaaac 
      aacaaaaaa tcgtcaata agctcattc aattcttag 
      ttacgttag ttcaaccgt ctagaatga cagttggtg 
      taaaaaggg ggtaccaat tgatgttgc acgaaagtc 
      caaataaga aaagcaaac ttagtaata acggaaaaa 
      gtaacatta atgcagagg gacccacaa ttcatgaat 
      tggacagcg aactggatt taacaccaa atttatacg 
      aagaggaag taccagttt gtgtcagta caccagaag 
      aattaatta ttccggatc aggatataa gcgaagaaa 
      ctttgatta atagtttaa aagatgaag catcaccag 
      ttggtgatg ttaatgttg acaataacg gcagttttg 
      ataatcaaa aagtgtttt tgaatccgt caactcagg 
      acacgttgt taagtaatt taataaaaa ataatacga 
      ctaaagatg gggctaaga atggaaaac ctataaacg 
      cagccactt cactgtcat ctgctcctg ttactagta 
      acaatatgg ccattgcca ctcgtgata cgaatttgt 
      gtgatgtaa ttaagagta tgccaggac ctagcgata 
      cttatacca tggatttaa ataaccacg tggaagcca 
      ctcaacagg aattagacc aattcaaag atattttaa 
      atggatata atccatttg ttgatgcta atgccttac 
      taggggaat taaacaaga tccctcaaa acttcaaag 
      atgtctatc tctttaatg gcgattctc ccgtttatg 
      atctgaatg ttaatgatg ataataaaa atgaaaact 
      tgatcgatc cttctgatc cgttggttt ctgggtctg 
      aattaaccc catatggtg gtaattttg attttactg 
      aactatttg atgatacca ttaatgaag ggaatgtac 
      agacaactg aagctacat cttcaactg ttaatacac 
      cgcaaatta ttaaagaag aacctattt ttccggcta 
      tacaaaaca aaaagtaat ttctaattt taggccaga 
      gatagaaac aaatgtaca aaattggat cgattgagt 
      tgatccgtc ataaaattc gcatttttt ttataaact 
      agcgtaatg tggtaggga gactactgt gacttcaga 
      ttgtgaata caagaaaat tccctagta tttttgttt 
      ggcgatttt atttttcaa ctgatacga tctaatgta 
      aggcttggt ttgttttgt atattttag cccattgta 
      tttttcaat atataaata ttgctattt ttgtatatc 
      tatataaga cagggaatt tctctattt atgtatttt 
      tttaaactt gataggttt tataagttc acactgaaa 
      gtccgttgt ttcatgcag caaaaaatc aaacaaatc 
      aacgcgata tagatcata acagtcaaa attaacacc 
      aagtgttgg ttttttgtt ggataaaat gttgtactt 
      gtgtagtaa aggatacag ctatcgcca cttttcaca 
      acggttttt ctgtagagt ctcaagaag ttcacctca 
      aaatttttg tggcgataa aaatgttcc gagatttaa 
      caattgctt gatgctatt tccttgtaa gcttttata 
      ggcatcaac cagactgta ggtttccta aaactttta 
      atcttgatt attcaagtt actagttag ggagtgcta 
      aattttgtc caaagtaaa atgatcatc cgtactttt 
      gtccaccat aaaaatgtc caaaatagc agaatgtaa 
      ttttatcca acgtaaaag catccgaag tatttttgt 
      cgaacgtaa atgagcatc cgtattttt gtggaccat 
      aaaagtatc caaaatttc tagtgttat tttcaatac 
      caacatggt aacaaaact gtctaaagt aaaatttaa 
      atttgtaag tgtctctag taacacact gtatcacta 
      ttttccgac cacaagcat aaatggctt atatttttt 
      ttctacaaa gctatttac gatatccag tcctccgtc 
      aatgctgcc atgatccta gatcgttta acgttttta 
      cattggatt attttactt tgtacaatt ttgtatgcg 
      tccacaagt taacttttt ttaatctta atatatttt 
      ttgttatta t
    • >TC034534 RB
      gtagtgcca ccactcacc atctgtttg tttaatgtt 
      caggtcaat aaacacacg ttagggagt attgtacaa 
      taagaaaag gtcctacaa tgcaaccgt taggggaaa 
      atgctgcaa acactccag cctttttag gtaaattat 
      ggaaaatgg ttaatgacc catcgaccg accacctga 
      tctgctgga gtccgtccg gcactagct ttgtgatac 
      caaatcaag ctcaatttt ggtatgaac ttcttcctc 
      tctattaca aacacaata acatgagta gtttcgtga 
      ggcagttaa acatgtatg gtttccata aaatgtcga 
      cggttgaaa atggtacaa tggactcag ataaagatg 
      aaattcaat tttcacatc cgtattttc ttaaagacc 
      agcctgaac tcttgcgca atattaaaa gaaaagcaa 
      ccacttcta aaacttcta acgaaaata acaataaac 
      atgacgagt taactaaag ttctaagcg atgttaaac 
      aactcagag gacgtcaag tcagtgttg ataatcaac 
      taaatgcaa tgaaacaag aaaatgcgc ttttgtggc 
      gcgaagtgg ctattctta ggcagaaac atttgaaac 
      aacaaaaaa tcgtcaata agctcattc aattcttag 
      ttacgttag ttcaaccgt ctagaatga cagttggtg 
      taaaaaggg ggtaccaat tgatgttgc acgaaagtc 
      caaataaga aaagcaaac ttagtaata acggaaaaa 
      gtaacatta atgcagagg gacccacaa ttcatgaat 
      tggacagcg aactggatt taacaccaa atttatacg 
      aagaggaag taccagttt gtgtcagta caccagaag 
      aattaatta ttccggatc aggatataa gcgaagaaa 
      ctttgatta atagtttaa aagatgaag catcaccag 
      ttggtgatg ttaatgttg acaataacg gcagttttg 
      ataatcaaa aagtgtttt tgaatccgt caactcagg 
      acacgttgt taagtaatt taataaaaa ataatacga 
      ctaaagatg gggctaaga atggaaaac ctataaacg 
      cagccactt cactgtcat ctgctcctg ttactagta 
      acaatatgg ccattgcca ctcgtgata cgaatttgt 
      gtgatgtaa ttaagagta tgccaggac ctagcgata 
      cttatacca tggatttaa ataaccacg tggaagcca 
      ctcaacagg aattagacc aattcaaag atattttaa 
      atggatata atccatttg ttgatgcta atgccttac 
      tagggctct ttaatggcg attctcccg tttatgatc 
      tgaatgtta atgatgata ataaaaatg aaaacttga 
      tcgatcctt ctgatccgt tggtttctg ggtctgaat 
      taaccccat atggtggta attttgatt ttactgaac 
      tatttgatg ataccatta atgaaggga atgtacaga 
      caactgaag ctacatctt caactgtta atacaccgc 
      aaattatta aagaagaac ctatttttc cggctatac 
      aaaacaaaa agtaatttc taattttag gccagagat 
      agaaacaaa tgtacaaaa ttggatcga ttgagttga 
      tccgtcata aaattcgca tttttttta taaactagc 
      gtaatgtgg tagggagac tactgtgac ttcagattg 
      tgaatacaa gaaaattcc ctagtattt ttgtttggc 
      gattttatt tttcaactg atacgatct aatgtaagg 
      cttggtttg ttttgtata ttttagccc attgtattt 
      ttcaatata taaatattg ctatttttg tatatctat 
      ataagacag ggaatttct ctatttatg tattttttt 
      aaacttgat aggttttat aagttcaca ctgaaagtc 
      cgttgtttc atgcagcaa aaaatcaaa caaatcaac 
      gcgatatag atcataaca gtcaaaatt aacaccaag 
      tgttggttt tttgttgga taaaatgtt gtacttgtg 
      tagtaaagg atacagcta tcgccactt ttcacaacg 
      gtttttctg tagagtctc aagaagttc acctcaaaa 
      tttttgtgg cgataaaaa tgttccgag atttaacaa 
      ttgcttgat gctatttcc ttgtaagct tttataggc 
      atcaaccag actgtaggt ttcctaaaa cttttaatc 
      ttgattatt caagttact agttaggga gtgctaaat 
      tttgtccaa agtaaaatg atcatccgt acttttgtc 
      caccataaa aatgtccaa aatagcaga atgtaattt 
      tatccaacg taaaagcat ccgaagtat ttttgtcga 
      acgtaaatg agcatccgt atttttgtg gaccataaa 
      agtatccaa aatttctag tgttatttt caataccaa 
      catggtaac aaaactgtc taaagtaaa atttaaatt 
      tgtaagtgt ctctagtaa cacactgta tcactattt 
      tccgaccac aagcataaa tggcttata ttttttttc 
      tacaaagct atttacgat atccagtcc tccgtcaat 
      gctgccatg atcctagat cgtttaacg tttttacat 
      tggattatt ttactttgt acaattttg tatgcgtcc 
      acaagttaa cttttttta atcttaata tattttttg 
      ttattat
    • >TC034534 RC
      gtagtgcca ccactcacc atctgtttg tttaatgtt 
      caggtcaat aaacacacg ttagggagt attgtacaa 
      taagaaaag gtcctacaa tgcaaccgt taggggaaa 
      atgctgcaa acactccag cctttttag gtaaattat 
      ggaaaatgg ttaatgacc catcgaccg accacctga 
      tctgctgga gtccgtccg gcactagct ttgtgatac 
      caaatcaag ctcaatttt ggtatgaac ttcttcctc 
      tctattaca aacacaata acatgagta gtttcgtga 
      ggcagttaa acatgtatg gtttccata aaatgtcga 
      cggttgaaa atggtacaa tggactcag ataaagatg 
      aaattcaat tttcacatc cgtattttc ttaaagacc 
      agcctgaac tcttgcgca atattaaaa gaaaagcaa 
      ccacttcta aaacttcta acgaaaata acaataaac 
      atgacgagt taactaaag ttctaagcg atgttaaac 
      aactcagag gacgtcaag tcagtgttg ataatcaac 
      taaatgcaa tgaaacaag aaaatgcgc ttttgtggc 
      gcgaagtgg ctattctta ggcagaaac atttgaaac 
      aacaaaaaa tcgtcaata agctcattc aattcttag 
      ttacgttag ttcaaccgt ctagaatga cagttggtg 
      taaaaaggg ggtaccaat tgatgttgc acgaaagtc 
      caaataaga aaagcaaac ttagtaata acggaaaaa 
      gtaacatta atgcagagg gacccacaa ttcatgaat 
      tggacagcg aactggatt taacaccaa atttatacg 
      aagaggaag taccagttg tggaaggaa gcaacatcg 
      aaattgaaa atgacttgt gtgtcagta caccagaag 
      aattaatta ttccggatc aggatataa gcgaagaaa 
      ctttgatta atagtttaa aagatgaag catcaccag 
      ttggtgatg ttaatgttg acaataacg gcagttttg 
      ataatcaaa aagtgtttt tgaatccgt caactcagg 
      acacgttgt taagtaatt taataaaaa ataatacga 
      ctaaagatg gggctaaga atggaaaac ctataaacg 
      cagccactt cactgtcat ctgctcctg ttactagta 
      acaatatgg ccattgcca ctcgtgata cgaatttgt 
      gtgatgtaa ttaagagta tgccaggac ctagcgata 
      cttatacca tggatttaa ataaccacg tggaagcca 
      ctcaacagg aattagacc aattcaaag atattttaa 
      atggatata atccatttg ttgatgcta atgccttac 
      taggggaat taaacaaga tccctcaaa acttcaaag 
      atgtctatc tctttaatg gcgattctc ccgtttatg 
      atctgaatg ttaatgatg ataataaaa atgaaaact 
      tgatcgatc cttctgatc cgttggttt ctgggtctg 
      aattaaccc catatggtg gtaattttg attttactg 
      aactatttg atgatacca ttaatgaag ggaatgtac 
      agacaactg aagctacat cttcaactg ttaatacac 
      cgcaaatta ttaaagaag aacctattt ttccggcta 
      tacaaaaca aaaagtaat ttctaattt taggccaga 
      gatagaaac aaatgtaca aaattggat cgattgagt 
      tgatccgtc ataaaattc gcatttttt ttataaact 
      agcgtaatg tggtaggga gactactgt gacttcaga 
      ttgtgaata caagaaaat tccctagta tttttgttt 
      ggcgatttt atttttcaa ctgatacga tctaatgta 
      aggcttggt ttgttttgt atattttag cccattgta 
      tttttcaat atataaata ttgctattt ttgtatatc 
      tatataaga cagggaatt tctctattt atgtatttt 
      tttaaactt gataggttt tataagttc acactgaaa 
      gtccgttgt ttcatgcag caaaaaatc aaacaaatc 
      aacgcgata tagatcata acagtcaaa attaacacc 
      aagtgttgg ttttttgtt ggataaaat gttgtactt 
      gtgtagtaa aggatacag ctatcgcca cttttcaca 
      acggttttt ctgtagagt ctcaagaag ttcacctca 
      aaatttttg tggcgataa aaatgttcc gagatttaa 
      caattgctt gatgctatt tccttgtaa gcttttata 
      ggcatcaac cagactgta ggtttccta aaactttta 
      atcttgatt attcaagtt actagttag ggagtgcta 
      aattttgtc caaagtaaa atgatcatc cgtactttt 
      gtccaccat aaaaatgtc caaaatagc agaatgtaa 
      ttttatcca acgtaaaag catccgaag tatttttgt 
      cgaacgtaa atgagcatc cgtattttt gtggaccat 
      aaaagtatc caaaatttc tagtgttat tttcaatac 
      caacatggt aacaaaact gtctaaagt aaaatttaa 
      atttgtaag tgtctctag taacacact gtatcacta 
      ttttccgac cacaagcat aaatggctt atatttttt 
      ttctacaaa gctatttac gatatccag tcctccgtc 
      aatgctgcc atgatccta gatcgttta acgttttta 
      cattggatt attttactt tgtacaatt ttgtatgcg 
      tccacaagt taacttttt ttaatctta atatatttt 
      ttgttatta t
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