| General information | |||
| TC034534 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 8486167..8496961 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034534 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00291 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
vitellogenic egg chamber early potentiallydegenerated - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC034534 RA
MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV CVSTPEELI IPDQDISEE TLINSLKDE ASPVGDVNV DNNGSFDNQ KVFLNPSTQ DTLLSNLIK NNTTKDGAK NGKPINAAT SLSSAPVTS NNMAIATRD TNLCDVIKS MPGPSDTYT MDLNNHVEA TQQELDQFK DILNGYNPF VDANALLGE LNKIPQNFK DVYLFNGDS PVYDLNVND DNKNENLID PSDPLVSGS ELTPYGGNF DFTELFDDT INEGNVQTT EATSSTVNT PQIIKEEPI FPAIQNKK
- >TC034534 RB
MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV CVSTPEELI IPDQDISEE TLINSLKDE ASPVGDVNV DNNGSFDNQ KVFLNPSTQ DTLLSNLIK NNTTKDGAK NGKPINAAT SLSSAPVTS NNMAIATRD TNLCDVIKS MPGPSDTYT MDLNNHVEA TQQELDQFK DILNGYNPF VDANALLGL FNGDSPVYD LNVNDDNKN ENLIDPSDP LVSGSELTP YGGNFDFTE LFDDTINEG NVQTTEATS STVNTPQII KEEPIFPAI QNKK
- >TC034534 RC
MQPLGENAA NTPAFLGKL WKMVNDPST DHLICWSPS GTSFVIPNQ AQFWYELLP LYYKHNNMS SFVRQLNMY GFHKMSTVE NGTMDSDKD EIQFSHPYF LKDQPELLR NIKRKATTS KTSNENNNK HDELTKVLS DVKQLRGRQ VSVDNQLNA MKQENALLW REVAILRQK HLKQQKIVN KLIQFLVTL VQPSRMTVG VKRGYQLML HESPNKKSK LSNNGKSNI NAEGPTIHE LDSELDLTP NLYEEEVPV VEGSNIEIE NDLCVSTPE ELIIPDQDI SEETLINSL KDEASPVGD VNVDNNGSF DNQKVFLNP STQDTLLSN LIKNNTTKD GAKNGKPIN AATSLSSAP VTSNNMAIA TRDTNLCDV IKSMPGPSD TYTMDLNNH VEATQQELD QFKDILNGY NPFVDANAL LGELNKIPQ NFKDVYLFN GDSPVYDLN VNDDNKNEN LIDPSDPLV SGSELTPYG GNFDFTELF DDTINEGNV QTTEATSST VNTPQIIKE EPIFPAIQN KK
- >TC034534 RA
atgcaaccg ttaggggaa aatgctgca aacactcca gccttttta ggtaaatta tggaaaatg gttaatgac ccatcgacc gaccacctg atctgctgg agtccgtcc ggcactagc tttgtgata ccaaatcaa gctcaattt tggtatgaa cttcttcct ctctattac aaacacaat aacatgagt agtttcgtg aggcagtta aacatgtat ggtttccat aaaatgtcg acggttgaa aatggtaca atggactca gataaagat gaaattcaa ttttcacat ccgtatttt cttaaagac cagcctgaa ctcttgcgc aatattaaa agaaaagca accacttct aaaacttct aacgaaaat aacaataaa catgacgag ttaactaaa gttctaagc gatgttaaa caactcaga ggacgtcaa gtcagtgtt gataatcaa ctaaatgca atgaaacaa gaaaatgcg cttttgtgg cgcgaagtg gctattctt aggcagaaa catttgaaa caacaaaaa atcgtcaat aagctcatt caattctta gttacgtta gttcaaccg tctagaatg acagttggt gtaaaaagg gggtaccaa ttgatgttg cacgaaagt ccaaataag aaaagcaaa cttagtaat aacggaaaa agtaacatt aatgcagag ggacccaca attcatgaa ttggacagc gaactggat ttaacacca aatttatac gaagaggaa gtaccagtt tgtgtcagt acaccagaa gaattaatt attccggat caggatata agcgaagaa actttgatt aatagttta aaagatgaa gcatcacca gttggtgat gttaatgtt gacaataac ggcagtttt gataatcaa aaagtgttt ttgaatccg tcaactcag gacacgttg ttaagtaat ttaataaaa aataatacg actaaagat ggggctaag aatggaaaa cctataaac gcagccact tcactgtca tctgctcct gttactagt aacaatatg gccattgcc actcgtgat acgaatttg tgtgatgta attaagagt atgccagga cctagcgat acttatacc atggattta aataaccac gtggaagcc actcaacag gaattagac caattcaaa gatatttta aatggatat aatccattt gttgatgct aatgcctta ctaggggaa ttaaacaag atccctcaa aacttcaaa gatgtctat ctctttaat ggcgattct cccgtttat gatctgaat gttaatgat gataataaa aatgaaaac ttgatcgat ccttctgat ccgttggtt tctgggtct gaattaacc ccatatggt ggtaatttt gattttact gaactattt gatgatacc attaatgaa gggaatgta cagacaact gaagctaca tcttcaact gttaataca ccgcaaatt attaaagaa gaacctatt tttccggct atacaaaac aaaaagtaa
- >TC034534 RB
atgcaaccg ttaggggaa aatgctgca aacactcca gccttttta ggtaaatta tggaaaatg gttaatgac ccatcgacc gaccacctg atctgctgg agtccgtcc ggcactagc tttgtgata ccaaatcaa gctcaattt tggtatgaa cttcttcct ctctattac aaacacaat aacatgagt agtttcgtg aggcagtta aacatgtat ggtttccat aaaatgtcg acggttgaa aatggtaca atggactca gataaagat gaaattcaa ttttcacat ccgtatttt cttaaagac cagcctgaa ctcttgcgc aatattaaa agaaaagca accacttct aaaacttct aacgaaaat aacaataaa catgacgag ttaactaaa gttctaagc gatgttaaa caactcaga ggacgtcaa gtcagtgtt gataatcaa ctaaatgca atgaaacaa gaaaatgcg cttttgtgg cgcgaagtg gctattctt aggcagaaa catttgaaa caacaaaaa atcgtcaat aagctcatt caattctta gttacgtta gttcaaccg tctagaatg acagttggt gtaaaaagg gggtaccaa ttgatgttg cacgaaagt ccaaataag aaaagcaaa cttagtaat aacggaaaa agtaacatt aatgcagag ggacccaca attcatgaa ttggacagc gaactggat ttaacacca aatttatac gaagaggaa gtaccagtt tgtgtcagt acaccagaa gaattaatt attccggat caggatata agcgaagaa actttgatt aatagttta aaagatgaa gcatcacca gttggtgat gttaatgtt gacaataac ggcagtttt gataatcaa aaagtgttt ttgaatccg tcaactcag gacacgttg ttaagtaat ttaataaaa aataatacg actaaagat ggggctaag aatggaaaa cctataaac gcagccact tcactgtca tctgctcct gttactagt aacaatatg gccattgcc actcgtgat acgaatttg tgtgatgta attaagagt atgccagga cctagcgat acttatacc atggattta aataaccac gtggaagcc actcaacag gaattagac caattcaaa gatatttta aatggatat aatccattt gttgatgct aatgcctta ctagggctc tttaatggc gattctccc gtttatgat ctgaatgtt aatgatgat aataaaaat gaaaacttg atcgatcct tctgatccg ttggtttct gggtctgaa ttaacccca tatggtggt aattttgat tttactgaa ctatttgat gataccatt aatgaaggg aatgtacag acaactgaa gctacatct tcaactgtt aatacaccg caaattatt aaagaagaa cctattttt ccggctata caaaacaaa aagtaa
- >TC034534 RC
atgcaaccg ttaggggaa aatgctgca aacactcca gccttttta ggtaaatta tggaaaatg gttaatgac ccatcgacc gaccacctg atctgctgg agtccgtcc ggcactagc tttgtgata ccaaatcaa gctcaattt tggtatgaa cttcttcct ctctattac aaacacaat aacatgagt agtttcgtg aggcagtta aacatgtat ggtttccat aaaatgtcg acggttgaa aatggtaca atggactca gataaagat gaaattcaa ttttcacat ccgtatttt cttaaagac cagcctgaa ctcttgcgc aatattaaa agaaaagca accacttct aaaacttct aacgaaaat aacaataaa catgacgag ttaactaaa gttctaagc gatgttaaa caactcaga ggacgtcaa gtcagtgtt gataatcaa ctaaatgca atgaaacaa gaaaatgcg cttttgtgg cgcgaagtg gctattctt aggcagaaa catttgaaa caacaaaaa atcgtcaat aagctcatt caattctta gttacgtta gttcaaccg tctagaatg acagttggt gtaaaaagg gggtaccaa ttgatgttg cacgaaagt ccaaataag aaaagcaaa cttagtaat aacggaaaa agtaacatt aatgcagag ggacccaca attcatgaa ttggacagc gaactggat ttaacacca aatttatac gaagaggaa gtaccagtt gtggaagga agcaacatc gaaattgaa aatgacttg tgtgtcagt acaccagaa gaattaatt attccggat caggatata agcgaagaa actttgatt aatagttta aaagatgaa gcatcacca gttggtgat gttaatgtt gacaataac ggcagtttt gataatcaa aaagtgttt ttgaatccg tcaactcag gacacgttg ttaagtaat ttaataaaa aataatacg actaaagat ggggctaag aatggaaaa cctataaac gcagccact tcactgtca tctgctcct gttactagt aacaatatg gccattgcc actcgtgat acgaatttg tgtgatgta attaagagt atgccagga cctagcgat acttatacc atggattta aataaccac gtggaagcc actcaacag gaattagac caattcaaa gatatttta aatggatat aatccattt gttgatgct aatgcctta ctaggggaa ttaaacaag atccctcaa aacttcaaa gatgtctat ctctttaat ggcgattct cccgtttat gatctgaat gttaatgat gataataaa aatgaaaac ttgatcgat ccttctgat ccgttggtt tctgggtct gaattaacc ccatatggt ggtaatttt gattttact gaactattt gatgatacc attaatgaa gggaatgta cagacaact gaagctaca tcttcaact gttaataca ccgcaaatt attaaagaa gaacctatt tttccggct atacaaaac aaaaagtaa
- >TC034534 RA
gtagtgcca ccactcacc atctgtttg tttaatgtt caggtcaat aaacacacg ttagggagt attgtacaa taagaaaag gtcctacaa tgcaaccgt taggggaaa atgctgcaa acactccag cctttttag gtaaattat ggaaaatgg ttaatgacc catcgaccg accacctga tctgctgga gtccgtccg gcactagct ttgtgatac caaatcaag ctcaatttt ggtatgaac ttcttcctc tctattaca aacacaata acatgagta gtttcgtga ggcagttaa acatgtatg gtttccata aaatgtcga cggttgaaa atggtacaa tggactcag ataaagatg aaattcaat tttcacatc cgtattttc ttaaagacc agcctgaac tcttgcgca atattaaaa gaaaagcaa ccacttcta aaacttcta acgaaaata acaataaac atgacgagt taactaaag ttctaagcg atgttaaac aactcagag gacgtcaag tcagtgttg ataatcaac taaatgcaa tgaaacaag aaaatgcgc ttttgtggc gcgaagtgg ctattctta ggcagaaac atttgaaac aacaaaaaa tcgtcaata agctcattc aattcttag ttacgttag ttcaaccgt ctagaatga cagttggtg taaaaaggg ggtaccaat tgatgttgc acgaaagtc caaataaga aaagcaaac ttagtaata acggaaaaa gtaacatta atgcagagg gacccacaa ttcatgaat tggacagcg aactggatt taacaccaa atttatacg aagaggaag taccagttt gtgtcagta caccagaag aattaatta ttccggatc aggatataa gcgaagaaa ctttgatta atagtttaa aagatgaag catcaccag ttggtgatg ttaatgttg acaataacg gcagttttg ataatcaaa aagtgtttt tgaatccgt caactcagg acacgttgt taagtaatt taataaaaa ataatacga ctaaagatg gggctaaga atggaaaac ctataaacg cagccactt cactgtcat ctgctcctg ttactagta acaatatgg ccattgcca ctcgtgata cgaatttgt gtgatgtaa ttaagagta tgccaggac ctagcgata cttatacca tggatttaa ataaccacg tggaagcca ctcaacagg aattagacc aattcaaag atattttaa atggatata atccatttg ttgatgcta atgccttac taggggaat taaacaaga tccctcaaa acttcaaag atgtctatc tctttaatg gcgattctc ccgtttatg atctgaatg ttaatgatg ataataaaa atgaaaact tgatcgatc cttctgatc cgttggttt ctgggtctg aattaaccc catatggtg gtaattttg attttactg aactatttg atgatacca ttaatgaag ggaatgtac agacaactg aagctacat cttcaactg ttaatacac cgcaaatta ttaaagaag aacctattt ttccggcta tacaaaaca aaaagtaat ttctaattt taggccaga gatagaaac aaatgtaca aaattggat cgattgagt tgatccgtc ataaaattc gcatttttt ttataaact agcgtaatg tggtaggga gactactgt gacttcaga ttgtgaata caagaaaat tccctagta tttttgttt ggcgatttt atttttcaa ctgatacga tctaatgta aggcttggt ttgttttgt atattttag cccattgta tttttcaat atataaata ttgctattt ttgtatatc tatataaga cagggaatt tctctattt atgtatttt tttaaactt gataggttt tataagttc acactgaaa gtccgttgt ttcatgcag caaaaaatc aaacaaatc aacgcgata tagatcata acagtcaaa attaacacc aagtgttgg ttttttgtt ggataaaat gttgtactt gtgtagtaa aggatacag ctatcgcca cttttcaca acggttttt ctgtagagt ctcaagaag ttcacctca aaatttttg tggcgataa aaatgttcc gagatttaa caattgctt gatgctatt tccttgtaa gcttttata ggcatcaac cagactgta ggtttccta aaactttta atcttgatt attcaagtt actagttag ggagtgcta aattttgtc caaagtaaa atgatcatc cgtactttt gtccaccat aaaaatgtc caaaatagc agaatgtaa ttttatcca acgtaaaag catccgaag tatttttgt cgaacgtaa atgagcatc cgtattttt gtggaccat aaaagtatc caaaatttc tagtgttat tttcaatac caacatggt aacaaaact gtctaaagt aaaatttaa atttgtaag tgtctctag taacacact gtatcacta ttttccgac cacaagcat aaatggctt atatttttt ttctacaaa gctatttac gatatccag tcctccgtc aatgctgcc atgatccta gatcgttta acgttttta cattggatt attttactt tgtacaatt ttgtatgcg tccacaagt taacttttt ttaatctta atatatttt ttgttatta t
- >TC034534 RB
gtagtgcca ccactcacc atctgtttg tttaatgtt caggtcaat aaacacacg ttagggagt attgtacaa taagaaaag gtcctacaa tgcaaccgt taggggaaa atgctgcaa acactccag cctttttag gtaaattat ggaaaatgg ttaatgacc catcgaccg accacctga tctgctgga gtccgtccg gcactagct ttgtgatac caaatcaag ctcaatttt ggtatgaac ttcttcctc tctattaca aacacaata acatgagta gtttcgtga ggcagttaa acatgtatg gtttccata aaatgtcga cggttgaaa atggtacaa tggactcag ataaagatg aaattcaat tttcacatc cgtattttc ttaaagacc agcctgaac tcttgcgca atattaaaa gaaaagcaa ccacttcta aaacttcta acgaaaata acaataaac atgacgagt taactaaag ttctaagcg atgttaaac aactcagag gacgtcaag tcagtgttg ataatcaac taaatgcaa tgaaacaag aaaatgcgc ttttgtggc gcgaagtgg ctattctta ggcagaaac atttgaaac aacaaaaaa tcgtcaata agctcattc aattcttag ttacgttag ttcaaccgt ctagaatga cagttggtg taaaaaggg ggtaccaat tgatgttgc acgaaagtc caaataaga aaagcaaac ttagtaata acggaaaaa gtaacatta atgcagagg gacccacaa ttcatgaat tggacagcg aactggatt taacaccaa atttatacg aagaggaag taccagttt gtgtcagta caccagaag aattaatta ttccggatc aggatataa gcgaagaaa ctttgatta atagtttaa aagatgaag catcaccag ttggtgatg ttaatgttg acaataacg gcagttttg ataatcaaa aagtgtttt tgaatccgt caactcagg acacgttgt taagtaatt taataaaaa ataatacga ctaaagatg gggctaaga atggaaaac ctataaacg cagccactt cactgtcat ctgctcctg ttactagta acaatatgg ccattgcca ctcgtgata cgaatttgt gtgatgtaa ttaagagta tgccaggac ctagcgata cttatacca tggatttaa ataaccacg tggaagcca ctcaacagg aattagacc aattcaaag atattttaa atggatata atccatttg ttgatgcta atgccttac tagggctct ttaatggcg attctcccg tttatgatc tgaatgtta atgatgata ataaaaatg aaaacttga tcgatcctt ctgatccgt tggtttctg ggtctgaat taaccccat atggtggta attttgatt ttactgaac tatttgatg ataccatta atgaaggga atgtacaga caactgaag ctacatctt caactgtta atacaccgc aaattatta aagaagaac ctatttttc cggctatac aaaacaaaa agtaatttc taattttag gccagagat agaaacaaa tgtacaaaa ttggatcga ttgagttga tccgtcata aaattcgca tttttttta taaactagc gtaatgtgg tagggagac tactgtgac ttcagattg tgaatacaa gaaaattcc ctagtattt ttgtttggc gattttatt tttcaactg atacgatct aatgtaagg cttggtttg ttttgtata ttttagccc attgtattt ttcaatata taaatattg ctatttttg tatatctat ataagacag ggaatttct ctatttatg tattttttt aaacttgat aggttttat aagttcaca ctgaaagtc cgttgtttc atgcagcaa aaaatcaaa caaatcaac gcgatatag atcataaca gtcaaaatt aacaccaag tgttggttt tttgttgga taaaatgtt gtacttgtg tagtaaagg atacagcta tcgccactt ttcacaacg gtttttctg tagagtctc aagaagttc acctcaaaa tttttgtgg cgataaaaa tgttccgag atttaacaa ttgcttgat gctatttcc ttgtaagct tttataggc atcaaccag actgtaggt ttcctaaaa cttttaatc ttgattatt caagttact agttaggga gtgctaaat tttgtccaa agtaaaatg atcatccgt acttttgtc caccataaa aatgtccaa aatagcaga atgtaattt tatccaacg taaaagcat ccgaagtat ttttgtcga acgtaaatg agcatccgt atttttgtg gaccataaa agtatccaa aatttctag tgttatttt caataccaa catggtaac aaaactgtc taaagtaaa atttaaatt tgtaagtgt ctctagtaa cacactgta tcactattt tccgaccac aagcataaa tggcttata ttttttttc tacaaagct atttacgat atccagtcc tccgtcaat gctgccatg atcctagat cgtttaacg tttttacat tggattatt ttactttgt acaattttg tatgcgtcc acaagttaa cttttttta atcttaata tattttttg ttattat
- >TC034534 RC
gtagtgcca ccactcacc atctgtttg tttaatgtt caggtcaat aaacacacg ttagggagt attgtacaa taagaaaag gtcctacaa tgcaaccgt taggggaaa atgctgcaa acactccag cctttttag gtaaattat ggaaaatgg ttaatgacc catcgaccg accacctga tctgctgga gtccgtccg gcactagct ttgtgatac caaatcaag ctcaatttt ggtatgaac ttcttcctc tctattaca aacacaata acatgagta gtttcgtga ggcagttaa acatgtatg gtttccata aaatgtcga cggttgaaa atggtacaa tggactcag ataaagatg aaattcaat tttcacatc cgtattttc ttaaagacc agcctgaac tcttgcgca atattaaaa gaaaagcaa ccacttcta aaacttcta acgaaaata acaataaac atgacgagt taactaaag ttctaagcg atgttaaac aactcagag gacgtcaag tcagtgttg ataatcaac taaatgcaa tgaaacaag aaaatgcgc ttttgtggc gcgaagtgg ctattctta ggcagaaac atttgaaac aacaaaaaa tcgtcaata agctcattc aattcttag ttacgttag ttcaaccgt ctagaatga cagttggtg taaaaaggg ggtaccaat tgatgttgc acgaaagtc caaataaga aaagcaaac ttagtaata acggaaaaa gtaacatta atgcagagg gacccacaa ttcatgaat tggacagcg aactggatt taacaccaa atttatacg aagaggaag taccagttg tggaaggaa gcaacatcg aaattgaaa atgacttgt gtgtcagta caccagaag aattaatta ttccggatc aggatataa gcgaagaaa ctttgatta atagtttaa aagatgaag catcaccag ttggtgatg ttaatgttg acaataacg gcagttttg ataatcaaa aagtgtttt tgaatccgt caactcagg acacgttgt taagtaatt taataaaaa ataatacga ctaaagatg gggctaaga atggaaaac ctataaacg cagccactt cactgtcat ctgctcctg ttactagta acaatatgg ccattgcca ctcgtgata cgaatttgt gtgatgtaa ttaagagta tgccaggac ctagcgata cttatacca tggatttaa ataaccacg tggaagcca ctcaacagg aattagacc aattcaaag atattttaa atggatata atccatttg ttgatgcta atgccttac taggggaat taaacaaga tccctcaaa acttcaaag atgtctatc tctttaatg gcgattctc ccgtttatg atctgaatg ttaatgatg ataataaaa atgaaaact tgatcgatc cttctgatc cgttggttt ctgggtctg aattaaccc catatggtg gtaattttg attttactg aactatttg atgatacca ttaatgaag ggaatgtac agacaactg aagctacat cttcaactg ttaatacac cgcaaatta ttaaagaag aacctattt ttccggcta tacaaaaca aaaagtaat ttctaattt taggccaga gatagaaac aaatgtaca aaattggat cgattgagt tgatccgtc ataaaattc gcatttttt ttataaact agcgtaatg tggtaggga gactactgt gacttcaga ttgtgaata caagaaaat tccctagta tttttgttt ggcgatttt atttttcaa ctgatacga tctaatgta aggcttggt ttgttttgt atattttag cccattgta tttttcaat atataaata ttgctattt ttgtatatc tatataaga cagggaatt tctctattt atgtatttt tttaaactt gataggttt tataagttc acactgaaa gtccgttgt ttcatgcag caaaaaatc aaacaaatc aacgcgata tagatcata acagtcaaa attaacacc aagtgttgg ttttttgtt ggataaaat gttgtactt gtgtagtaa aggatacag ctatcgcca cttttcaca acggttttt ctgtagagt ctcaagaag ttcacctca aaatttttg tggcgataa aaatgttcc gagatttaa caattgctt gatgctatt tccttgtaa gcttttata ggcatcaac cagactgta ggtttccta aaactttta atcttgatt attcaagtt actagttag ggagtgcta aattttgtc caaagtaaa atgatcatc cgtactttt gtccaccat aaaaatgtc caaaatagc agaatgtaa ttttatcca acgtaaaag catccgaag tatttttgt cgaacgtaa atgagcatc cgtattttt gtggaccat aaaagtatc caaaatttc tagtgttat tttcaatac caacatggt aacaaaact gtctaaagt aaaatttaa atttgtaag tgtctctag taacacact gtatcacta ttttccgac cacaagcat aaatggctt atatttttt ttctacaaa gctatttac gatatccag tcctccgtc aatgctgcc atgatccta gatcgttta acgttttta cattggatt attttactt tgtacaatt ttgtatgcg tccacaagt taacttttt ttaatctta atatatttt ttgttatta t
