Status
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Gene information
General information
TC001714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8103857..8121557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001714
    iBeetle screen iB_00287

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001714 RA
      MRKAIKAIH TSGHAHVEN EFSLSKALE KLGSKDRED 
      VIGEAFDKF SVVTNQLSL LKKTLMQNL NNIVMFPLD 
      NLLKGDLRG IKGDLKRPF DKASKDYDS KYAKIEKEK 
      KQQAKEAGL IRSEITAAE IADEMEKER RLFQLQMCE 
      YLIKYNEIK TKKGIDLLQ NLVEYYHAQ INYFQDGLK 
      TIQHFGFYV ADLSVKLQK IKQVQDDEK RKLTDLRNL 
      IKSTPSIEK EVSLPADKG AAGYSLHQL QGDKHHGVT 
      RSGHLLKKS EGKMRRVWQ KRKCRVQAE GYLEICHAD 
      ESKPPTKVN LLTCQIKVV PDDKRGFDL ISYNRTYHF 
      QAEDEADQH AWMSVLINC KEMALMKAF DNSGKTGSD 
      KVNHSLIEL QQAIIRYIQ KIPGNDRCC DCNSQNDAT 
      WLSTNFGII VCIECSGIH RDLGVHISR IQSLTLDNV 
      GTSQLVLAR FMTNHSFND VMEAQLSPN EKLEPSSSM 
      EERLNFIRA KYVDKQFAD KTCDNEIKK LYYLEEAVN 
      NGDLGLLLQ MFAENVDFG ATLPSSKNG ETALHRAIL 
      NENGRTLHI VDFLIQNMS SSAIDKATS PPSDLENCG 
      SNTALHLCA IYDKVECMK LLLRSGANA NVKNSQNKT 
      PMDIAQELG HHTCEDLLM NTANRQKNF FDNINIDWN 
      LSHDDGSTD FSDDETIIE DRNGSLTPE KKNRSRPPS 
      YTGGDSPVT LRSRSSTCD SLQLGSSPN SNSRQMPPP 
      PPPQARKPE FLVHSSLKK RVAPLPPPP NSMQLQYGT 
      LPSSHSRTP SDPIAAGYH TLSHTHKRS PSSDSSSGR 
      SVIPIGNKL VISSDCKSS DKLGIVRRP QNPPPPAPN 
      PSNRLSNGR STESISSMA SDVDVPGHN PVPPPRRAS 
      DSPPHRVSS SPISTGSLC SPRSIITGR LPYTPNGSN 
      GLRRCRALY NCSADNDDE LSFSEGEII IVINEHTDD 
      ENWMEGMVE NEPERKGMF PVSFVHRLP D
    CDS sequence
    • >TC001714 RA
      atgaggaaa gcaattaag gcaatccac acatctgga 
      cacgcacat gtcgaaaat gaattttca ttgagtaaa 
      gctttggaa aaactgggg agtaaggat cgggaggat 
      gtgatagga gaagctttc gataagttt tcggtggta 
      acaaatcag ttgtcgctt ttgaagaaa acgttaatg 
      caaaatttg aataacatc gttatgttt cctttggat 
      aatttattg aagggtgat ttgaggggc ataaagggg 
      gatttgaaa cggccgttc gataaagca tcgaaggat 
      tatgacagc aaatatgcc aaaatagaa aaagaaaag 
      aagcaacaa gccaaagaa gcgggtcta attagatca 
      gaaattaca gcagcagaa atagccgac gaaatggaa 
      aaagaacgg agattattt cagctgcaa atgtgtgaa 
      tatctaatc aaatataat gaaattaaa accaagaag 
      ggtattgat ttgctccaa aacttagta gaatattac 
      cacgctcaa atcaattat tttcaagac ggattaaaa 
      acaatccaa cactttgga ttctacgta gccgacctt 
      agcgtaaaa ctacagaaa atcaagcaa gtgcaagat 
      gacgagaaa agaaaattg acggattta cgcaactta 
      attaaatca acgccaagt atcgaaaag gaagtgagt 
      ttaccggct gataaaggc gctgccggt tactcgtta 
      caccagctc caaggagac aaacaccac ggtgtgaca 
      agaagcggc cacctactt aaaaaatcc gaaggaaaa 
      atgaggcga gtctggcaa aaaagaaaa tgtagagtg 
      caagccgaa ggttattta gaaatctgt cacgctgat 
      gaaagtaaa ccacccact aaagtaaat ttgctcacc 
      tgccaaata aaagttgtg cctgacgat aaacgcggc 
      tttgattta atttcatac aaccggacg tatcatttc 
      caagcggaa gacgaagcg gatcaacac gcttggatg 
      tcggtttta atcaattgt aaagaaatg gccctaatg 
      aaggctttc gataatagc ggaaaaacg gggagtgat 
      aaagtcaac catagttta attgagttg caacaggcg 
      ataatacgg tatatacag aaaattccg gggaatgat 
      cgctgttgt gattgcaat tcgcaaaat gatgccact 
      tggttgtcg accaatttc ggtattatt gtctgtatc 
      gagtgctca ggtattcac agggatttg ggtgtacat 
      atttccagg attcagtct ttgactctt gataacgtt 
      ggaactagt caattggtc ctcgctagg tttatgaca 
      aatcatagt tttaatgat gtgatggaa gctcagttg 
      tcacctaat gaaaaactc gaaccgtcc agttctatg 
      gaagaaaga cttaatttt attagggcg aaatacgta 
      gacaaacaa ttcgctgac aaaacgtgt gacaatgaa 
      atcaaaaaa ctttactat ttagaagaa gcggttaat 
      aacggagat ttaggttta cttctacag atgtttgcc 
      gaaaatgtt gattttggt gctactttg ccctcctca 
      aagaacggc gaaacggcg ttacatcga gcgattctg 
      aacgagaat ggaagaacg ttacatata gttgatttt 
      ctcattcaa aacatgtcc agttcagcg attgacaaa 
      gcaacgagt cctccctcc gatttagaa aattgtgga 
      tccaacact gctctgcat ttatgtgcc atttacgat 
      aaagtagaa tgtatgaag cttctatta cgaagtggt 
      gctaacgct aatgttaag aattcacaa aacaaaacc 
      ccgatggat attgctcag gagttgggc catcacacg 
      tgtgaagat ctgctcatg aacactgca aataggcaa 
      aagaacttc ttcgataat attaatatc gactggaat 
      ttatcgcac gacgatggt tcgaccgat ttttcggac 
      gatgaaaca ataatagag gataggaat ggttcctta 
      acaccagaa aagaaaaac cgttcacgc ccacctagt 
      tatacaggg ggcgattcg ccagtaact ttaaggtca 
      cgatcgtcc acttgtgac agtttacag ttaggaagt 
      agtcctaat agtaacagt aggcaaatg ccaccacca 
      ccaccccca caggctcgg aaacctgag tttttagtc 
      cattcgagt ttaaagaag agagtggcg cctcttcca 
      ccgccccca aattcaatg cagttacaa tacggaaca 
      ttacctagt agccatagt cgaacacca tccgatcct 
      atagcagca ggctaccat accttaagt catacccat 
      aagcgttca ccgagcagt gattcaagc tcaggtaga 
      tctgtgata cctatagga aataaacta gtcatatct 
      tcagattgt aagagctct gataaattg gggatagtt 
      cgaaggcca caaaatcct cctccacca gctccaaac 
      ccttcgaat cgtctttca aacggtcgt tcaacggaa 
      agcatttct tcgatggca tctgatgtt gatgtccca 
      ggacataat cccgtacca ccaccaaga agggcatca 
      gactctcct cctcataga gtatcatct agtccaatc 
      tctacagga tctctttgc tctccacgc tcaataatc 
      actggaaga ttaccttac acaccaaac ggaagcaat 
      gggttgcga aggtgtaga gctttatac aactgcagt 
      gcagataac gatgacgaa ttatcgttt agtgaagga 
      gaaattata attgttata aatgaacac acagatgat 
      gagaattgg atggaagga atggtcgag aatgaacct 
      gagagaaag ggaatgttc cctgtaagt tttgttcat 
      agattaccc gactag
    mRNA sequence
    • >TC001714 RA
      aacagttgg cataagtgt gtaatagaa gtgatcaac 
      aatctctga aatagcaaa gcaatgaat gcaatatat 
      caataaata gtttgtgat aatggatgt gcgggtgcg 
      aaaagtgca gtgaaatat tggaagtgc gtctggtgg 
      tggttttta cgatgtgtt ggttgcgta aagctctta 
      cgagcacgt aaaaagtga taaactgat gctattttg 
      acttatttc tattagaat acatgatgc ctacgatag 
      tcggagttg ccgaatttg taaaagaaa ctaaggatg 
      attaccatt ccccaatta cttcaacat ttgtgtcta 
      gaatgccac aatttaaaa ataccattt ctcaattgg 
      aagagacgc tggattgcg atcgagatg ggctgacta 
      aaatgagga aagcaatta aggcaatcc acacatctg 
      gacacgcac atgtcgaaa atgaatttt cattgagta 
      aagctttgg aaaaactgg ggagtaagg atcgggagg 
      atgtgatag gagaagctt tcgataagt tttcggtgg 
      taacaaatc agttgtcgc ttttgaaga aaacgttaa 
      tgcaaaatt tgaataaca tcgttatgt ttcctttgg 
      ataatttat tgaagggtg atttgaggg gcataaagg 
      gggatttga aacggccgt tcgataaag catcgaagg 
      attatgaca gcaaatatg ccaaaatag aaaaagaaa 
      agaagcaac aagccaaag aagcgggtc taattagat 
      cagaaatta cagcagcag aaatagccg acgaaatgg 
      aaaaagaac ggagattat ttcagctgc aaatgtgtg 
      aatatctaa tcaaatata atgaaatta aaaccaaga 
      agggtattg atttgctcc aaaacttag tagaatatt 
      accacgctc aaatcaatt attttcaag acggattaa 
      aaacaatcc aacactttg gattctacg tagccgacc 
      ttagcgtaa aactacaga aaatcaagc aagtgcaag 
      atgacgaga aaagaaaat tgacggatt tacgcaact 
      taattaaat caacgccaa gtatcgaaa aggaagtga 
      gtttaccgg ctgataaag gcgctgccg gttactcgt 
      tacaccagc tccaaggag acaaacacc acggtgtga 
      caagaagcg gccacctac ttaaaaaat ccgaaggaa 
      aaatgaggc gagtctggc aaaaaagaa aatgtagag 
      tgcaagccg aaggttatt tagaaatct gtcacgctg 
      atgaaagta aaccaccca ctaaagtaa atttgctca 
      cctgccaaa taaaagttg tgcctgacg ataaacgcg 
      gctttgatt taatttcat acaaccgga cgtatcatt 
      tccaagcgg aagacgaag cggatcaac acgcttgga 
      tgtcggttt taatcaatt gtaaagaaa tggccctaa 
      tgaaggctt tcgataata gcggaaaaa cggggagtg 
      ataaagtca accatagtt taattgagt tgcaacagg 
      cgataatac ggtatatac agaaaattc cggggaatg 
      atcgctgtt gtgattgca attcgcaaa atgatgcca 
      cttggttgt cgaccaatt tcggtatta ttgtctgta 
      tcgagtgct caggtattc acagggatt tgggtgtac 
      atatttcca ggattcagt ctttgactc ttgataacg 
      ttggaacta gtcaattgg tcctcgcta ggtttatga 
      caaatcata gttttaatg atgtgatgg aagctcagt 
      tgtcaccta atgaaaaac tcgaaccgt ccagttcta 
      tggaagaaa gacttaatt ttattaggg cgaaatacg 
      tagacaaac aattcgctg acaaaacgt gtgacaatg 
      aaatcaaaa aactttact atttagaag aagcggtta 
      ataacggag atttaggtt tacttctac agatgtttg 
      ccgaaaatg ttgattttg gtgctactt tgccctcct 
      caaagaacg gcgaaacgg cgttacatc gagcgattc 
      tgaacgaga atggaagaa cgttacata tagttgatt 
      ttctcattc aaaacatgt ccagttcag cgattgaca 
      aagcaacga gtcctccct ccgatttag aaaattgtg 
      gatccaaca ctgctctgc atttatgtg ccatttacg 
      ataaagtag aatgtatga agcttctat tacgaagtg 
      gtgctaacg ctaatgtta agaattcac aaaacaaaa 
      ccccgatgg atattgctc aggagttgg gccatcaca 
      cgtgtgaag atctgctca tgaacactg caaataggc 
      aaaagaact tcttcgata atattaata tcgactgga 
      atttatcgc acgacgatg gttcgaccg atttttcgg 
      acgatgaaa caataatag aggatagga atggttcct 
      taacaccag aaaagaaaa accgttcac gcccaccta 
      gttatacag ggggcgatt cgccagtaa ctttaaggt 
      cacgatcgt ccacttgtg acagtttac agttaggaa 
      gtagtccta atagtaaca gtaggcaaa tgccaccac 
      caccacccc cacaggctc ggaaacctg agtttttag 
      tccattcga gtttaaaga agagagtgg cgcctcttc 
      caccgcccc caaattcaa tgcagttac aatacggaa 
      cattaccta gtagccata gtcgaacac catccgatc 
      ctatagcag caggctacc ataccttaa gtcataccc 
      ataagcgtt caccgagca gtgattcaa gctcaggta 
      gatctgtga tacctatag gaaataaac tagtcatat 
      cttcagatt gtaagagct ctgataaat tggggatag 
      ttcgaaggc cacaaaatc ctcctccac cagctccaa 
      acccttcga atcgtcttt caaacggtc gttcaacgg 
      aaagcattt cttcgatgg catctgatg ttgatgtcc 
      caggacata atcccgtac caccaccaa gaagggcat 
      cagactctc ctcctcata gagtatcat ctagtccaa 
      tctctacag gatctcttt gctctccac gctcaataa 
      tcactggaa gattacctt acacaccaa acggaagca 
      atgggttgc gaaggtgta gagctttat acaactgca 
      gtgcagata acgatgacg aattatcgt ttagtgaag 
      gagaaatta taattgtta taaatgaac acacagatg 
      atgagaatt ggatggaag gaatggtcg agaatgaac 
      ctgagagaa agggaatgt tccctgtaa gttttgttc 
      atagattac ccgactagt agttttttt aacgttgaa 
      aaatctgtt gagtattat taaaattta aagattttt 
      atctatcgg ttcaaaaac tatttgaag ccatattcg 
      tcacgtaga aaatgacta taaattttc tgccatact 
      ttctgtatt tattttttt atcgtgaaa ttcgtattt 
      atttagcga catacatgg ttcagtgta tatataatt 
      tataattgt tgtgagcct ggagatgtt ttgttcctc 
      agattatta aatttgact gatgtacct atttgtcgc 
      ttgtttaca ttacataat ataaaagca tgtttgtgt 
      atataaagt catattttt ctagaaaaa ttaatttat 
      acaagtgat cgcctgtta gattctcaa accaaagaa 
      aagtctatc gttcttatg cagatgtag tatttatag 
      ctatgatta tataattgc cagtgtaca taatataag 
      ataagtgta ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY