| General information | |||
| TC001709 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7937490..7942890 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001709 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00284 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 33.33% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)
- >TC001709 RA
MLKNLLENR HSIGSVNND TCLTTRRAN ATVISNVYE GIPETILLN VITWILLIL LFAILRNRA WDYGRLALV HTEKWTQLF YKNTDDAVA VEESSADVS LIPDAGCLW FPSIFKISR ERIFTRCGP DASHYLSFQ EQLLFLSTT ITVFALCVI LPINFQGTL QGGKTTFGH TTLSNLEPS SYWLWVHVV ASFCFVPLT VLIMRKSSG RIPSASALT SRTVMITHI SHAHRNIDD IKNYFTVRF PDTEIKDIQ IAYRIKKLT ILEKQRALT HEAKMYCIL NNKPDLKVQ PYGCIICCP WKTQNALEY YTEEEARLT DLVVAERRK VLESPLGIA FITLNSEER AHHVIKSFT PGSLRHWLI TKAPSPSDI NWENLEISY RNWYSKAIL INAILFLVL FFLTTPVIV VNVFNNLTS AQDKFLSKL TPLLSDFLP TLLLLSVSA LMPVLVAYS DEWMSHWTK SKQNHATMH KAFFFLLFM VLILPSLGL TSAQAFVEW SLQTGNLTV RWECVFLAD KGAFFINYV ITSALIGTA LELLRFPEL AMYAFRLLR IKSEAEKTS IRKEIMSEF PFGIHYAWT LLIFTISTV YSLTCPLIT PFGLLYLCL KHFVDKYNI YYVYRPITM CGEGQQIHA DAVRMVRVA ILLLQLIMA AFSFIRGGF NIMCAVSFI AFIVTVTFF FFLSPFPSC RPIQNNNTA LPEFSEQYI APVLLSSQN IEQGDEYIV SSPSSYGSS NINDIGVRL MSEIGSSVA
- >TC001709 RA
atgctgaag aatcttctg gagaaccgc cactccatc ggatcggtc aataacgac acatgtttg acaacacgg cgtgccaat gccactgtc atcagcaac gtgtacgag ggtatcccc gagacgatc ctcctgaac gtcatcacc tggatcttg ctgatcctc ctctttgcc attttgcgc aacagggcg tgggattac ggccgtttg gccctcgtc cacacggaa aaatggacc cagctgttc tacaaaaac acagatgat gccgtcgcc gttgaagaa tcaagcgcc gacgtgtcg ttaatcccc gacgcgggc tgtctctgg ttcccttct attttcaaa atcagcaga gagcggatt ttcacacgt tgtggcccg gacgcctca cactatctc tctttccaa gaacaactc cttttctta tccactact ataacagtg ttcgctctt tgcgttata ctcccgatc aacttccaa gggactctc caagggggt aaaaccact tttggtcac accactctg agcaacctg gagcccagc tcctactgg ttgtgggta catgttgtc gctagtttt tgcttcgtc cctttgact gttttgatc atgcggaag agctccggt cggattccc agtgcctcg gcactgacc tcacggact gttatgata acacacatc tcacacgca caccgaaat atcgacgat attaagaat tatttcact gtaaggttc ccggatacg gagatcaag gacatacaa attgcctac aggattaag aaattgacg atacttgag aaacagcga gccctcact catgaggct aagatgtac tgtattttg aacaacaaa cctgacttg aaggtgcaa ccgtacggt tgtattatt tgttgtccg tggaagaca caaaacgca cttgagtac tacaccgaa gaggaggca cgcctcact gatttagtt gtggcggag cgacgaaaa gtcttagag agtccgctt ggtatcgct ttcattacc ttaaattca gaagaacgt gcgcaccac gtcatcaaa tcattcact ccggggtcg ctccgccac tggctcata acaaaagcc ccttcgccg tcagacatc aactgggag aacttagaa atttcgtac cggaactgg tactcgaaa gcaatcctc atcaacgca atactcttc ctggtgctg tttttcctc acaactccc gtgattgtg gtcaacgtc ttcaacaac ttgacttcc gcacaagat aaattcttg agtaagctg acgcctctc ctttccgat tttcttcca actctcctc ctcctctcc gtgtccgcc ctaatgccg gttctggtg gcatattcg gacgaatgg atgtcgcac tggaccaaa tcgaagcaa aatcacgca actatgcac aaagccttt ttctttttg ctcttcatg gtgctcatc ctcccctcg ttgggtctg acgagtgcg caagcgttc gtcgaatgg tcccttcaa accggcaat ctgacggtg cgctgggag tgtgtcttt ttggccgac aaaggcgcg ttcttcatc aattacgtg atcacgtcc gctttgatc ggaacagct ttggagttg ttgcgcttc cccgagttg gccatgtac gcgttccgc ctccttcgg atcaaatca gaggcggag aagaccagc ataaggaag gaaatcatg tcggagttt ccttttggc attcactac gcctggacc ttgcttatc ttcaccata agcacggtg tacagtctc acgtgccct ttgataacg cccttcggg ttgctgtac ttgtgcttg aagcacttc gtcgataaa tacaatatt tactacgtc tacagaccc atcaccatg tgtggcgag ggccagcaa atccatgcc gatgccgtc aggatggtg cgcgtcgcc attttgctc ctccagcta ataatggcc gcgttttcc ttcattaga ggcggtttc aatataatg tgtgcggtt agttttatt gcgttcata gtcactgtc acgttcttc ttcttcttg agtccgttc ccctcttgt aggccgatt caaaataac aacacagct ttacccgag tttagtgag cagtacatc gcgccggtg ctattaagt tcgcaaaat attgagcaa ggggatgag tatattgta tcgtcacct tcgtcgtac ggttcctcg aatattaac gatattggt gttaggcta atgtctgaa attggttct tcggttgca taa
- >TC001709 RA
tgtcatttt tattacggt ggttgttca tgataaaat tattttaca gtagaaagt tgaaaatat gtacttggt ttacgtaat ttagttaag tttggtgct tctgaagtt gtttgtgat gaaattata gccctacaa aatgctgaa gaatcttct ggagaaccg ccactccat cggatcggt caataacga cacatgttt gacaacacg gcgtgccaa tgccactgt catcagcaa cgtgtacga gggtatccc cgagacgat cctcctgaa cgtcatcac ctggatctt gctgatcct cctctttgc cattttgcg caacagggc gtgggatta cggccgttt ggccctcgt ccacacgga aaaatggac ccagctgtt ctacaaaaa cacagatga tgccgtcgc cgttgaaga atcaagcgc cgacgtgtc gttaatccc cgacgcggg ctgtctctg gttcccttc tattttcaa aatcagcag agagcggat tttcacacg ttgtggccc ggacgcctc acactatct ctctttcca agaacaact ccttttctt atccactac tataacagt gttcgctct ttgcgttat actcccgat caacttcca agggactct ccaaggggg taaaaccac ttttggtca caccactct gagcaacct ggagcccag ctcctactg gttgtgggt acatgttgt cgctagttt ttgcttcgt ccctttgac tgttttgat catgcggaa gagctccgg tcggattcc cagtgcctc ggcactgac ctcacggac tgttatgat aacacacat ctcacacgc acaccgaaa tatcgacga tattaagaa ttatttcac tgtaaggtt cccggatac ggagatcaa ggacataca aattgccta caggattaa gaaattgac gatacttga gaaacagcg agccctcac tcatgaggc taagatgta ctgtatttt gaacaacaa acctgactt gaaggtgca accgtacgg ttgtattat ttgttgtcc gtggaagac acaaaacgc acttgagta ctacaccga agaggaggc acgcctcac tgatttagt tgtggcgga gcgacgaaa agtcttaga gagtccgct tggtatcgc tttcattac cttaaattc agaagaacg tgcgcacca cgtcatcaa atcattcac tccggggtc gctccgcca ctggctcat aacaaaagc cccttcgcc gtcagacat caactggga gaacttaga aatttcgta ccggaactg gtactcgaa agcaatcct catcaacgc aatactctt cctggtgct gtttttcct cacaactcc cgtgattgt ggtcaacgt cttcaacaa cttgacttc cgcacaaga taaattctt gagtaagct gacgcctct cctttccga ttttcttcc aactctcct cctcctctc cgtgtccgc cctaatgcc ggttctggt ggcatattc ggacgaatg gatgtcgca ctggaccaa atcgaagca aaatcacgc aactatgca caaagcctt tttcttttt gctcttcat ggtgctcat cctcccctc gttgggtct gacgagtgc gcaagcgtt cgtcgaatg gtcccttca aaccggcaa tctgacggt gcgctggga gtgtgtctt tttggccga caaaggcgc gttcttcat caattacgt gatcacgtc cgctttgat cggaacagc tttggagtt gttgcgctt ccccgagtt ggccatgta cgcgttccg cctccttcg gatcaaatc agaggcgga gaagaccag cataaggaa ggaaatcat gtcggagtt tccttttgg cattcacta cgcctggac cttgcttat cttcaccat aagcacggt gtacagtct cacgtgccc tttgataac gcccttcgg gttgctgta cttgtgctt gaagcactt cgtcgataa atacaatat ttactacgt ctacagacc catcaccat gtgtggcga gggccagca aatccatgc cgatgccgt caggatggt gcgcgtcgc cattttgct cctccagct aataatggc cgcgttttc cttcattag aggcggttt caatataat gtgtgcggt tagttttat tgcgttcat agtcactgt cacgttctt cttcttctt gagtccgtt cccctcttg taggccgat tcaaaataa caacacagc tttacccga gtttagtga gcagtacat cgcgccggt gctattaag ttcgcaaaa tattgagca aggggatga gtatattgt atcgtcacc ttcgtcgta cggttcctc gaatattaa cgatattgg tgttaggct aatgtctga aattggttc ttcggttgc ataaatttc ttttctgct tctggtatg gctgtgatt atttaggaa gtgtttcac ccgttggtt ccacctttc atgtgataa ttccctaac ttttaaagt attgactca ttcgtttta attcctagt ccttctttc ggtcgctta acttgaaat tgcaatgaa cggtgataa cacgtaact gtaggaaga ttataatat ataactatt tatttgtaa agtctattt tgtatattt tagcacata actgtagag ttcttgtat aaatatata attgctatg tta
