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Gene information
General information
TC001709 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7937490..7942890
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001709
    iBeetle screen iB_00284

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 33.33% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001709 RA
      MLKNLLENR HSIGSVNND TCLTTRRAN ATVISNVYE 
GIPETILLN VITWILLIL LFAILRNRA WDYGRLALV 
HTEKWTQLF YKNTDDAVA VEESSADVS LIPDAGCLW 
FPSIFKISR ERIFTRCGP DASHYLSFQ EQLLFLSTT 
ITVFALCVI LPINFQGTL QGGKTTFGH TTLSNLEPS 
SYWLWVHVV ASFCFVPLT VLIMRKSSG RIPSASALT 
SRTVMITHI SHAHRNIDD IKNYFTVRF PDTEIKDIQ 
IAYRIKKLT ILEKQRALT HEAKMYCIL NNKPDLKVQ 
PYGCIICCP WKTQNALEY YTEEEARLT DLVVAERRK 
VLESPLGIA FITLNSEER AHHVIKSFT PGSLRHWLI 
TKAPSPSDI NWENLEISY RNWYSKAIL INAILFLVL 
FFLTTPVIV VNVFNNLTS AQDKFLSKL TPLLSDFLP 
TLLLLSVSA LMPVLVAYS DEWMSHWTK SKQNHATMH 
KAFFFLLFM VLILPSLGL TSAQAFVEW SLQTGNLTV 
RWECVFLAD KGAFFINYV ITSALIGTA LELLRFPEL 
AMYAFRLLR IKSEAEKTS IRKEIMSEF PFGIHYAWT 
LLIFTISTV YSLTCPLIT PFGLLYLCL KHFVDKYNI 
YYVYRPITM CGEGQQIHA DAVRMVRVA ILLLQLIMA 
AFSFIRGGF NIMCAVSFI AFIVTVTFF FFLSPFPSC 
RPIQNNNTA LPEFSEQYI APVLLSSQN IEQGDEYIV 
SSPSSYGSS NINDIGVRL MSEIGSSVA
    CDS sequence
    • >TC001709 RA
      atgctgaag aatcttctg gagaaccgc cactccatc 
ggatcggtc aataacgac acatgtttg acaacacgg 
cgtgccaat gccactgtc atcagcaac gtgtacgag 
ggtatcccc gagacgatc ctcctgaac gtcatcacc 
tggatcttg ctgatcctc ctctttgcc attttgcgc 
aacagggcg tgggattac ggccgtttg gccctcgtc 
cacacggaa aaatggacc cagctgttc tacaaaaac 
acagatgat gccgtcgcc gttgaagaa tcaagcgcc 
gacgtgtcg ttaatcccc gacgcgggc tgtctctgg 
ttcccttct attttcaaa atcagcaga gagcggatt 
ttcacacgt tgtggcccg gacgcctca cactatctc 
tctttccaa gaacaactc cttttctta tccactact 
ataacagtg ttcgctctt tgcgttata ctcccgatc 
aacttccaa gggactctc caagggggt aaaaccact 
tttggtcac accactctg agcaacctg gagcccagc 
tcctactgg ttgtgggta catgttgtc gctagtttt 
tgcttcgtc cctttgact gttttgatc atgcggaag 
agctccggt cggattccc agtgcctcg gcactgacc 
tcacggact gttatgata acacacatc tcacacgca 
caccgaaat atcgacgat attaagaat tatttcact 
gtaaggttc ccggatacg gagatcaag gacatacaa 
attgcctac aggattaag aaattgacg atacttgag 
aaacagcga gccctcact catgaggct aagatgtac 
tgtattttg aacaacaaa cctgacttg aaggtgcaa 
ccgtacggt tgtattatt tgttgtccg tggaagaca 
caaaacgca cttgagtac tacaccgaa gaggaggca 
cgcctcact gatttagtt gtggcggag cgacgaaaa 
gtcttagag agtccgctt ggtatcgct ttcattacc 
ttaaattca gaagaacgt gcgcaccac gtcatcaaa 
tcattcact ccggggtcg ctccgccac tggctcata 
acaaaagcc ccttcgccg tcagacatc aactgggag 
aacttagaa atttcgtac cggaactgg tactcgaaa 
gcaatcctc atcaacgca atactcttc ctggtgctg 
tttttcctc acaactccc gtgattgtg gtcaacgtc 
ttcaacaac ttgacttcc gcacaagat aaattcttg 
agtaagctg acgcctctc ctttccgat tttcttcca 
actctcctc ctcctctcc gtgtccgcc ctaatgccg 
gttctggtg gcatattcg gacgaatgg atgtcgcac 
tggaccaaa tcgaagcaa aatcacgca actatgcac 
aaagccttt ttctttttg ctcttcatg gtgctcatc 
ctcccctcg ttgggtctg acgagtgcg caagcgttc 
gtcgaatgg tcccttcaa accggcaat ctgacggtg 
cgctgggag tgtgtcttt ttggccgac aaaggcgcg 
ttcttcatc aattacgtg atcacgtcc gctttgatc 
ggaacagct ttggagttg ttgcgcttc cccgagttg 
gccatgtac gcgttccgc ctccttcgg atcaaatca 
gaggcggag aagaccagc ataaggaag gaaatcatg 
tcggagttt ccttttggc attcactac gcctggacc 
ttgcttatc ttcaccata agcacggtg tacagtctc 
acgtgccct ttgataacg cccttcggg ttgctgtac 
ttgtgcttg aagcacttc gtcgataaa tacaatatt 
tactacgtc tacagaccc atcaccatg tgtggcgag 
ggccagcaa atccatgcc gatgccgtc aggatggtg 
cgcgtcgcc attttgctc ctccagcta ataatggcc 
gcgttttcc ttcattaga ggcggtttc aatataatg 
tgtgcggtt agttttatt gcgttcata gtcactgtc 
acgttcttc ttcttcttg agtccgttc ccctcttgt 
aggccgatt caaaataac aacacagct ttacccgag 
tttagtgag cagtacatc gcgccggtg ctattaagt 
tcgcaaaat attgagcaa ggggatgag tatattgta 
tcgtcacct tcgtcgtac ggttcctcg aatattaac 
gatattggt gttaggcta atgtctgaa attggttct 
tcggttgca taa
    mRNA sequence
    • >TC001709 RA
      tgtcatttt tattacggt ggttgttca tgataaaat 
tattttaca gtagaaagt tgaaaatat gtacttggt 
ttacgtaat ttagttaag tttggtgct tctgaagtt 
gtttgtgat gaaattata gccctacaa aatgctgaa 
gaatcttct ggagaaccg ccactccat cggatcggt 
caataacga cacatgttt gacaacacg gcgtgccaa 
tgccactgt catcagcaa cgtgtacga gggtatccc 
cgagacgat cctcctgaa cgtcatcac ctggatctt 
gctgatcct cctctttgc cattttgcg caacagggc 
gtgggatta cggccgttt ggccctcgt ccacacgga 
aaaatggac ccagctgtt ctacaaaaa cacagatga 
tgccgtcgc cgttgaaga atcaagcgc cgacgtgtc 
gttaatccc cgacgcggg ctgtctctg gttcccttc 
tattttcaa aatcagcag agagcggat tttcacacg 
ttgtggccc ggacgcctc acactatct ctctttcca 
agaacaact ccttttctt atccactac tataacagt 
gttcgctct ttgcgttat actcccgat caacttcca 
agggactct ccaaggggg taaaaccac ttttggtca 
caccactct gagcaacct ggagcccag ctcctactg 
gttgtgggt acatgttgt cgctagttt ttgcttcgt 
ccctttgac tgttttgat catgcggaa gagctccgg 
tcggattcc cagtgcctc ggcactgac ctcacggac 
tgttatgat aacacacat ctcacacgc acaccgaaa 
tatcgacga tattaagaa ttatttcac tgtaaggtt 
cccggatac ggagatcaa ggacataca aattgccta 
caggattaa gaaattgac gatacttga gaaacagcg 
agccctcac tcatgaggc taagatgta ctgtatttt 
gaacaacaa acctgactt gaaggtgca accgtacgg 
ttgtattat ttgttgtcc gtggaagac acaaaacgc 
acttgagta ctacaccga agaggaggc acgcctcac 
tgatttagt tgtggcgga gcgacgaaa agtcttaga 
gagtccgct tggtatcgc tttcattac cttaaattc 
agaagaacg tgcgcacca cgtcatcaa atcattcac 
tccggggtc gctccgcca ctggctcat aacaaaagc 
cccttcgcc gtcagacat caactggga gaacttaga 
aatttcgta ccggaactg gtactcgaa agcaatcct 
catcaacgc aatactctt cctggtgct gtttttcct 
cacaactcc cgtgattgt ggtcaacgt cttcaacaa 
cttgacttc cgcacaaga taaattctt gagtaagct 
gacgcctct cctttccga ttttcttcc aactctcct 
cctcctctc cgtgtccgc cctaatgcc ggttctggt 
ggcatattc ggacgaatg gatgtcgca ctggaccaa 
atcgaagca aaatcacgc aactatgca caaagcctt 
tttcttttt gctcttcat ggtgctcat cctcccctc 
gttgggtct gacgagtgc gcaagcgtt cgtcgaatg 
gtcccttca aaccggcaa tctgacggt gcgctggga 
gtgtgtctt tttggccga caaaggcgc gttcttcat 
caattacgt gatcacgtc cgctttgat cggaacagc 
tttggagtt gttgcgctt ccccgagtt ggccatgta 
cgcgttccg cctccttcg gatcaaatc agaggcgga 
gaagaccag cataaggaa ggaaatcat gtcggagtt 
tccttttgg cattcacta cgcctggac cttgcttat 
cttcaccat aagcacggt gtacagtct cacgtgccc 
tttgataac gcccttcgg gttgctgta cttgtgctt 
gaagcactt cgtcgataa atacaatat ttactacgt 
ctacagacc catcaccat gtgtggcga gggccagca 
aatccatgc cgatgccgt caggatggt gcgcgtcgc 
cattttgct cctccagct aataatggc cgcgttttc 
cttcattag aggcggttt caatataat gtgtgcggt 
tagttttat tgcgttcat agtcactgt cacgttctt 
cttcttctt gagtccgtt cccctcttg taggccgat 
tcaaaataa caacacagc tttacccga gtttagtga 
gcagtacat cgcgccggt gctattaag ttcgcaaaa 
tattgagca aggggatga gtatattgt atcgtcacc 
ttcgtcgta cggttcctc gaatattaa cgatattgg 
tgttaggct aatgtctga aattggttc ttcggttgc 
ataaatttc ttttctgct tctggtatg gctgtgatt 
atttaggaa gtgtttcac ccgttggtt ccacctttc 
atgtgataa ttccctaac ttttaaagt attgactca 
ttcgtttta attcctagt ccttctttc ggtcgctta 
acttgaaat tgcaatgaa cggtgataa cacgtaact 
gtaggaaga ttataatat ataactatt tatttgtaa 
agtctattt tgtatattt tagcacata actgtagag 
ttcttgtat aaatatata attgctatg tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY