Status
Please wait ...
Gene information
General information
TC001698 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7751028..7754695
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001698
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00283

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001698 RA
      MRLNVALVL LFREIFCYN YTTKFIDVP LDHFSFTNN 
      ATFKLKYLI NDSFWIDDG PIFFYTGNE GAVETFAEN 
      TGFIFDIAP TFNALIVFA EHRYYGATL PFGNASFSN 
      PGHLGFLTS SQALADYVY LINHLQTTH QRSEYLSKV 
      PVVAFGGSY GGMLAAWLR MKYPASVVG AIAASAPIW 
      QFQGLTPCE NFNRIVSNV YKTAVDDDC SAPIQKSWK 
      IIRNITAND DGKAWLTKA WKLCSPLKS SDIDDLLEW 
      YSEILVNMA MVNYPYPTK FLAPLPAFP VRNFCYKLT 
      GEKITDDKS LVTAIGNAL EIYTNFTKA TKCNNINQT 
      AASLGEEGW DFQACTEMI MPMCSDDND MFENQSWDF 
      KKYSDKCYT KWGVRQTNA ELPILEYGG KDITAASNI 
      VFSNGLLDP WSSGGVLSN ISSTVSSVI IPEGAHHLD 
      LRGENRNDP KSVIEARQF HVSSIRKWI TDFYYSRDK 
      NYFKKSIFF NKITYTHGN NI
    CDS sequence
    • >TC001698 RA
      atgagactt aacgtagcg ttagtgctt ttattcaga 
      gagattttt tgctacaat tatacaacc aaattcatt 
      gatgtgcct ctagaccac ttcagtttt acaaacaat 
      gcgactttc aagctgaaa tacttaatc aatgatagt 
      ttttggatc gacgatggt ccgatattc ttctacacg 
      ggtaacgag ggggctgtt gagaccttc gccgaaaac 
      accggcttt atatttgat atagccccc acgttcaat 
      gctcttatc gttttcgcc gaacaccga tattatggg 
      gccaccctc ccgttcggt aacgcctct tttagcaac 
      cccggtcat ttggggttc ctgacctca agtcaagcc 
      ctcgctgat tacgtctat ttgatcaac cacctgcaa 
      acaacccac caacgctcc gagtactta agcaaagtc 
      cctgtcgtt gcctttggg ggctcgtac ggcggaatg 
      ttagcagcg tggttgcga atgaaatac ccagcgtct 
      gtcgtgggg gccattgcg gcctcggcc cccatctgg 
      cagtttcag gggctcacc ccctgcgag aatttcaac 
      cggattgtt agcaatgtg tacaaaacg gcagtcgac 
      gacgactgc agtgccccc attcaaaag tcgtggaaa 
      attataagg aacatcaca gcaaatgac gacggcaaa 
      gcctggctt acaaaagca tggaaattg tgctcgccg 
      ctcaaaagc tcagacatt gatgatttg cttgaatgg 
      tattcggaa attttggtc aacatggca atggtcaat 
      tacccatac cctacgaag tttctggcc cccttaccc 
      gcatttccg gtcagaaat ttttgctat aaactgacc 
      ggagagaaa atcactgat gataagtcg ctcgtgact 
      gcgatcggt aacgctcta gaaatttac acgaacttt 
      actaaagca accaagtgt aataacatc aaccaaact 
      gcggcgtct ttgggcgaa gagggctgg gatttccag 
      gcttgtacc gagatgata atgcccatg tgctcggac 
      gataacgac atgttcgaa aaccagtct tgggatttt 
      aaaaaatat tccgacaag tgttacacc aaatggggg 
      gtcaggcaa acaaacgcc gaattaccg attttggaa 
      tacgggggg aaggacata accgccgcc agtaacatt 
      gttttcagc aacggactg ctcgaccct tggtcaagc 
      gggggcgtt ttaagcaac atttcctct acagtttcc 
      tccgtcata atcccagaa ggggcgcac cacttggac 
      ttacgcggc gaaaatcgc aacgacccc aaatcggtc 
      atcgaagcc cgtcagttt cacgtcagc agcataagg 
      aaatggatc acggatttt tactactcg cgcgataaa 
      aactacttc aaaaaatcg attttcttc aacaagatt 
      acgtacaca catggaaac aacatttag
    mRNA sequence
    • >TC001698 RA
      ggtcaaagt gcatcactt aactcctga ttgaacaat 
      tcttcagtc accacaatg agacttaac gtagcgtta 
      gtgctttta ttcagagag attttttgc tacaattat 
      acaaccaaa ttcattgat gtgcctcta gaccacttc 
      agttttaca aacaatgcg actttcaag ctgaaatac 
      ttaatcaat gatagtttt tggatcgac gatggtccg 
      atattcttc tacacgggt aacgagggg gctgttgag 
      accttcgcc gaaaacacc ggctttata tttgatata 
      gcccccacg ttcaatgct cttatcgtt ttcgccgaa 
      caccgatat tatggggcc accctcccg ttcggtaac 
      gcctctttt agcaacccc ggtcatttg gggttcctg 
      acctcaagt caagccctc gctgattac gtctatttg 
      atcaaccac ctgcaaaca acccaccaa cgctccgag 
      tacttaagc aaagtccct gtcgttgcc tttgggggc 
      tcgtacggc ggaatgtta gcagcgtgg ttgcgaatg 
      aaataccca gcgtctgtc gtgggggcc attgcggcc 
      tcggccccc atctggcag tttcagggg ctcaccccc 
      tgcgagaat ttcaaccgg attgttagc aatgtgtac 
      aaaacggca gtcgacgac gactgcagt gcccccatt 
      caaaagtcg tggaaaatt ataaggaac atcacagca 
      aatgacgac ggcaaagcc tggcttaca aaagcatgg 
      aaattgtgc tcgccgctc aaaagctca gacattgat 
      gatttgctt gaatggtat tcggaaatt ttggtcaac 
      atggcaatg gtcaattac ccataccct acgaagttt 
      ctggccccc ttacccgca tttccggtc agaaatttt 
      tgctataaa ctgaccgga gagaaaatc actgatgat 
      aagtcgctc gtgactgcg atcggtaac gctctagaa 
      atttacacg aactttact aaagcaacc aagtgtaat 
      aacatcaac caaactgcg gcgtctttg ggcgaagag 
      ggctgggat ttccaggct tgtaccgag atgataatg 
      cccatgtgc tcggacgat aacgacatg ttcgaaaac 
      cagtcttgg gattttaaa aaatattcc gacaagtgt 
      tacaccaaa tggggggtc aggcaaaca aacgccgaa 
      ttaccgatt ttggaatac ggggggaag gacataacc 
      gccgccagt aacattgtt ttcagcaac ggactgctc 
      gacccttgg tcaagcggg ggcgtttta agcaacatt 
      tcctctaca gtttcctcc gtcataatc ccagaaggg 
      gcgcaccac ttggactta cgcggcgaa aatcgcaac 
      gaccccaaa tcggtcatc gaagcccgt cagtttcac 
      gtcagcagc ataaggaaa tggatcacg gatttttac 
      tactcgcgc gataaaaac tacttcaaa aaatcgatt 
      ttcttcaac aagattacg tacacacat ggaaacaac 
      atttagaac tgtataaaa atttatttc ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY