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Gene information
General information
TC001697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7745228..7750981
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001697
    iBeetle screen iB_00282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 7

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection



    Pupal morphology
    head appendages orientation irregular - Number of animals showing the phenotype: 4

    head anteroposterior-axis shortened - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001697 RA
      MENAINLSS QAPESDMEC SDNDNDCFD DDDYYNIVD 
      DSEIEQIDP SKTDPENFK YDCLGEEQV ERLLNETVE 
      CLSYNLKIT PSLAKVLLL SHQWRVNKI IEKFRKDAS 
      ELLISSRIK PPVTPPSLS LSRYITCPV CVVVQPAEK 
      FFSLSCSHM FCKDCWVTH FEVQINQGI STAISCMAR 
      DCVVLAPED FVLKHLSRP SMREKYQQF TFQDYVKSH 
      PELRFCPGP NCPIVVHST EIRAKRATC SNCKTAFCF 
      QCGMDYHAP TECSIIKKW LTKCADDSE TANYISAHT 
      KDCPKCHIC IEKNGGCNH MQCYNCKHD FCWMCLGDW 
      KSHGSEYYE CSRYRENPN IAHESAHTQ AREALKKYL 
      HYYERWENH SKSLKLEEQ TLEKLRARI NQKVMAGVG 
      TWIDWQYLF TAASLLAKC RYTLQYTYP YAYYMDVGP 
      RKELFEYQQ AQLEAEIEN LSWKIERAE TTDRGDLEN 
      QMDIAEKRR TTLLKDFLD V
    CDS sequence
    • >TC001697 RA
      atggagaac gcaataaac ttgagctcc caggccccg 
      gagtcggac atggagtgc tcggataat gataatgac 
      tgttttgac gatgatgat tactacaac atcgtcgac 
      gacagcgag atcgagcaa attgatcca agcaaaacc 
      gacccggag aacttcaag tacgactgc ctgggcgag 
      gaacaagtg gagcggcta cttaacgaa actgttgaa 
      tgcctcagc tacaacctc aaaatcact ccttcccta 
      gccaaagtt ttgttgctc agccaccag tggcgcgtc 
      aacaaaatc attgaaaag ttccgcaag gacgcctcc 
      gaactgctc atcagttcg aggatcaag cccccagtc 
      acgcccccg agtctctcc ctttccaga tacatcacg 
      tgtccagtg tgtgtcgtt gtccaaccg gctgagaag 
      tttttcagc ctgtcgtgt tctcacatg ttttgtaag 
      gactgttgg gtcacccat tttgaagta caaataaat 
      cagggaata tcaacggct ataagttgt atggcccgc 
      gattgtgta gtgttggca cctgaagac ttcgttttg 
      aagcacttg tctcgtccc agcatgcgt gaaaaatac 
      cagcagttt acgttccaa gattacgtc aagtcccac 
      cctgagtta cgcttctgt ccgggccca aattgccca 
      attgttgta catagtacg gaaattagg gcaaaacgg 
      gcgacttgt tcaaactgc aaaaccgcc ttttgcttc 
      cagtgcgga atggattat cacgcacct accgagtgt 
      tcaataatc aagaagtgg ctaacgaag tgtgccgat 
      gacagtgaa accgctaac tatataagt gcacatacc 
      aaagactgt ccaaagtgc cacatttgc attgagaaa 
      aacggcggg tgcaatcat atgcaatgt tataattgt 
      aagcacgat ttttgctgg atgtgctta ggcgactgg 
      aagtcgcac ggctccgag tactacgag tgctcgcgc 
      taccgggaa aacccgaac attgctcac gaatccgcc 
      cacacccag gctcgcgag gcgctgaag aagtacctc 
      cactactac gagcggtgg gagaaccac tcgaagtcg 
      ctgaagttg gaggagcag acgctggag aagctgcga 
      gctcgcatc aaccaaaag gtgatggcg ggtgtgggc 
      acctggatc gattggcaa tatctcttc acggcggca 
      agtctcctt gcgaaatgt cgttacacg ttgcaatat 
      acatatcca tatgcctat tatatggat gtaggtcct 
      cgtaaagaa ctattcgaa tatcagcaa gcgcaatta 
      gaagctgaa atcgaaaac ttatcgtgg aaaatcgaa 
      cgcgcggaa accaccgat cggggcgat ctggagaac 
      cagatggac attgctgag aaacgaaga accacactt 
      ctaaaggac tttctagat gtttga
    mRNA sequence
    • >TC001697 RA
      agcgcgata aggcgccgc aaagctatc agtatcact 
      tactttgtt tatataatg atcctaagg attaataac 
      cattaacca aaattatat cttgaagcc gtcagtacg 
      aaatttgat ttttcgtct gcggacatt cggtcggcc 
      caccataat tttctaaag ttttttacc acaataacg 
      tcctcaaaa cgcgaatat tcaccaaaa tgttcccaa 
      tgtcttcag ggagattga cgccataac acagacatg 
      acagttcgc agtagttct ggaatcgta gaaatttgg 
      aaaatcgag ccaggcgct tactcggcg gctaccatg 
      actggttgg gggaaatgt cgcccattt gtgacgtca 
      cgcgccata ccaccggcc cggtcgggc gaaccgggc 
      ttgcaccaa cgccggtac acttgcagt tgccttatc 
      tcacttatc ttgtgtttt tgtggtagt tataagact 
      cagtgttgc gattttttg ttttgttta aatatagtt 
      cggtagctt tgggtgagt ccacgcaga gccgggctc 
      agccggtag gacaggacg ctgcattca ccaggtgca 
      tgtcagtgt cagtgtgct ataaaatgt gattgtgtt 
      tgtttggag aaatggcgg ccgtaatct gacacagta 
      atgacgact tcttgatcg cccgttaac cagcgccag 
      aatggagaa cgcaataaa cttgagctc ccaggcccc 
      ggagtcgga catggagtg ctcggataa tgataatga 
      ctgttttga cgatgatga ttactacaa catcgtcga 
      cgacagcga gatcgagca aattgatcc aagcaaaac 
      cgacccgga gaacttcaa gtacgactg cctgggcga 
      ggaacaagt ggagcggct acttaacga aactgttga 
      atgcctcag ctacaacct caaaatcac tccttccct 
      agccaaagt tttgttgct cagccacca gtggcgcgt 
      caacaaaat cattgaaaa gttccgcaa ggacgcctc 
      cgaactgct catcagttc gaggatcaa gcccccagt 
      cacgccccc gagtctctc cctttccag atacatcac 
      gtgtccagt gtgtgtcgt tgtccaacc ggctgagaa 
      gtttttcag cctgtcgtg ttctcacat gttttgtaa 
      ggactgttg ggtcaccca ttttgaagt acaaataaa 
      tcagggaat atcaacggc tataagttg tatggcccg 
      cgattgtgt agtgttggc acctgaaga cttcgtttt 
      gaagcactt gtctcgtcc cagcatgcg tgaaaaata 
      ccagcagtt tacgttcca agattacgt caagtccca 
      ccctgagtt acgcttctg tccgggccc aaattgccc 
      aattgttgt acatagtac ggaaattag ggcaaaacg 
      ggcgacttg ttcaaactg caaaaccgc cttttgctt 
      ccagtgcgg aatggatta tcacgcacc taccgagtg 
      ttcaataat caagaagtg gctaacgaa gtgtgccga 
      tgacagtga aaccgctaa ctatataag tgcacatac 
      caaagactg tccaaagtg ccacatttg cattgagaa 
      aaacggcgg gtgcaatca tatgcaatg ttataattg 
      taagcacga tttttgctg gatgtgctt aggcgactg 
      gaagtcgca cggctccga gtactacga gtgctcgcg 
      ctaccggga aaacccgaa cattgctca cgaatccgc 
      ccacaccca ggctcgcga ggcgctgaa gaagtacct 
      ccactacta cgagcggtg ggagaacca ctcgaagtc 
      gctgaagtt ggaggagca gacgctgga gaagctgcg 
      agctcgcat caaccaaaa ggtgatggc gggtgtggg 
      cacctggat cgattggca atatctctt cacggcggc 
      aagtctcct tgcgaaatg tcgttacac gttgcaata 
      tacatatcc atatgccta ttatatgga tgtaggtcc 
      tcgtaaaga actattcga atatcagca agcgcaatt 
      agaagctga aatcgaaaa cttatcgtg gaaaatcga 
      acgcgcgga aaccaccga tcggggcga tctggagaa 
      ccagatgga cattgctga gaaacgaag aaccacact 
      tctaaagga ctttctaga tgtttgata cagtttttt 
      aaattattt ccttcgttt gcaatgttg ccaatattg 
      ctttggatg aatgcggat tagtcgagg cgggttatt 
      ttattttgt tatcaccag agaactcat tggggaaaa 
      ttttacctt aagcacaca ctacagggt gacccagtc 
      ttgtggccc gaacaaagt agttatcca cattaaacg 
      tgcggtagc aagattttt caatattga agctcgaag 
      cacaagtga aatttttag acaaagacc gttcctcat 
      aattcaaat ttacatgac ggatctatg cgtttattt 
      ccatgagga gaaggtcga aaaaaatga ctttattta 
      tttattttt tttataaag aaagcagcg gaatcttct 
      ttcaacact aataaactt aattacatt gatttcaga 
      tttatctaa gctgcattt tcttagctt aaaatagtt 
      attaaggcc caatttctg gtacgatta aactaaact 
      tcaattaaa ttttatctc tccgttatg atgcaagtt 
      aattatttt tgcttaaag ttacccttt atatacgta 
      catattgta ctgtgagtt ttattatta atattacaa 
      atgttttgt ccagcatta tctggagtg tctgaatta 
      agtacactt ttattttaa aattacctc agctgaata 
      tttaatcat ggcaccgtt gtcggcagc ggaggcgct 
      gctaaaatt tatatattt tgtgattta cgctattat 
      ttataaact agaaatatt tctttttac tggggccac 
      aagattcgg aacacggtg taaacagat aaataaaaa 
      cttaaagta aaaaagtat tggcggggt ttttactta 
      tcaaacccc aggcacgac gaaagtcta aacaaaaag 
      ctgaacaat atagcaata aaacttata atgaagagc 
      cttgtagta gaagcaagg tttaaatta tgtctaatt 
      atttatgct ttatattag taacgattt aagagaaac 
      agctattgg aacaatagt agagtgtgc ccaaagtaa 
      aattttgtc cttgttgtt gcatccgat ggtaggtta 
      gtttgcggt ggcgaaatg tccgaatca agctactta 
      ttttattta aactagatc gatggtgga aggcgcagg 
      cgcagtagc gtccacaac cgcgcacat ctttccgac 
      ttgaagtca atcaaacta cgtattttt atatgagta 
      gtcgtaaca tattcatct cggaaagga ggaacatat 
      tgtgcaacg atattaatt ttggcgttt ctcttgtat 
      aataaaatt cctcttgtt atatttcat ttgttcgca 
      aataaacac tctcaatcg aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY