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Gene information
General information
TC031054
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002637,TC001683 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 1407..15304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031054
    iBeetle screen iB_00281
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Adult morphology
    hindwing irregular - Number of animals showing the phenotype: 4

    elytra not closed - Number of animals showing the phenotype: 4


    iBeetle screen iB_07039
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031054 RA
      MIFFKPSWV THEGSKPIF SVDIHPKGG RFATGGQGG 
SGGRIVIWN IAPVLSEND ESSLKIPKM LCQMDNHLA 
CVNVVRWSS EGHLLASGG DDKLVMIWR LTNEGSSSI 
FGSGKVNVE TWKCVHTLN SHNGDVLDL AWAPHDGWL 
ASGSVDNTV IIWNAHKFP EKVAVLKGH TGMVKGVTW 
DPVGKYIAS QSDDKSLRI WRTCDWVQQ EVVKDPFVD 
CSATTHVLR LSWSPDGQY LVSSHAMNG GGPTAQIIE 
REGWRQDKD FVGHRKAVT CVRFNSNIL KKISAGSPK 
SQQYCCCAI GSRDRSISV WLTSLKRPL VVIKDLFDD 
SVLDISWSS NGLYLFACS WDGSVACVI FDQREIGIP 
LSIKEKNDL YERIYHKSL QKSWNLSFS GTQIVENPE 
LLTAIDKIN ESSNNSVNL ELPKPASRD VSFESPKPN 
TTILVPANK QVETRLPSG KRRITPMLL TTVQTTTVE 
ATNSPPKTN LEVPTKEST SFSSSSPTK SKIVVETVV 
DQVKHPTFG GSSMKSSIL NDANKLISA PPGTQINQL 
TCCEVPGDQ PIVQFINVL DPLKLPSGT SVTKQCGTL 
KIQITNNCH KTSKGSLSS IEAYKNIDD KEPNWDTYI 
GSTIRCLAA NRKFIVACC EDLSINCYE IKSGARPLP 
PIIIEDVVS SISLSQDSH CLVLTKTGL LYMWNFELC 
KSILSRISI RSLLNNKGV VSSCGLTSS NLPVITLTD 
GRAYSYASD LQTWVQLSN PLDPLSRVG GSLTRRVSP 
TLPLASLQR VMPFHGPHD TLPSGVTLS FLENQITAS 
SIISSHDEY RHWLIATVN YLLDKGPEC RLRVILDEL 
LGPTYSSTA AKKSKNSDK ILGKPSHAF LQEILEIIK 
TKLPWQRLY KEYREQLDE ILHTM
    CDS sequence
    • >TC031054 RA
      atgattttt ttcaaaccg tcttgggta actcacgaa 
gggagtaaa ccaattttc tcagtggat atccacccc 
aaaggcgga cgctttgcc accggaggc caaggtggt 
tccggcggc cgcatagta atctggaat attgctccc 
gttttgtcc gaaaatgac gaaagtagc ctgaaaatt 
ccgaaaatg ttgtgccag atggacaat cacttggcg 
tgtgtaaat gtggtgagg tggagcagt gagggtcat 
ttattggcc tcagggggt gatgataaa cttgtcatg 
atttggagg cttacaaac gagggtagt tcatcgatt 
tttggtagt ggtaaagtc aatgtggag acttggaag 
tgtgttcac actctcaat tcacacaat ggggatgtt 
ttggatctg gcttgggcc ccacacgat ggttggttg 
gcatctgga agtgtggat aataccgta ataatctgg 
aatgctcat aagtttcca gaaaaggtt gctgttttg 
aaaggacac accggcatg gtcaagggt gttacttgg 
gatcccgtg ggcaaatat atagctagt cagtccgat 
gataaatca ttgagaatt tggagaact tgcgattgg 
gttcagcag gaggtggtt aaagaccct tttgttgat 
tgttcagca accacacat gttttaaga ctatcatgg 
tcacctgat gggcaatac cttgtctct tcacatgcc 
atgaatgga ggaggacct actgctcaa attattgaa 
cgagaaggt tggagacaa gataaggat tttgttggt 
caccgaaaa gcagtcaca tgtgttaga ttcaattca 
aacatttta aaaaagatc tctgctggc agtcccaag 
agtcagcaa tactgttgc tgtgcaatt ggttcaagg 
gacaggtct atcagtgta tggttgacg tctctgaaa 
cgaccatta gtggtaatc aaagatctg tttgacgac 
agtgtatta gacatttcg tggagtagc aatggttta 
tatctattc gcatgttct tgggacgga agcgtagct 
tgcgttata ttcgaccag cgagaaatc ggaatcccg 
ttatcaatt aaagaaaaa aatgatctc tatgaacgt 
atttatcat aaatctctg caaaaaagt tggaattta 
agctttagt gggactcaa atcgtggaa aatcccgaa 
cttttaact gccattgac aaaattaac gaaagtagt 
aataacagt gttaatctt gagttaccc aaaccggcg 
tcgagagat gtttcgttt gagtcacca aaaccaaac 
accacgatt ttagtacct gctaataag caagtggaa 
acgagactt ccttccgga aaaagacgt ataactccc 
atgttgttg accaccgtt caaacaacc accgtggaa 
gcaacaaac agtcctcca aagacaaat ctagaagtg 
ccgacaaaa gaaagcaca tcttttagt agttcttcg 
ccaacaaag agtaaaatt gttgttgag actgtagta 
gatcaagtc aaacacccc acttttggt ggctcatca 
atgaaaagt tcgatcttg aatgatgca aataaattg 
ataagtgcg cctccagga acccaaatt aatcagtta 
acttgttgc gaagtgccg ggagatcag cctatagtc 
cagtttatt aatgtttta gatccactg aaactgcct 
tcaggtaca tcagtcacc aaacagtgc gggacctta 
aaaatacag ataaccaat aactgccac aaaacttcg 
aaaggatct ttatcgagt attgaagcg tataaaaat 
atcgatgat aaagaacca aactgggat acgtacatt 
ggttccacc attaggtgt ttagcagca aacagaaaa 
tttatcgtg gcatgttgt gaagacctt agtattaat 
tgttacgag atcaagtca ggagcacgt ccattacct 
ccaataatt atcgaagat gtcgtttct tcaattagt 
ttatcacaa gatagccac tgtttagta ttgacaaag 
actggtctt ttgtacatg tggaatttc gagctttgt 
aagagcatt ttaagtagg atatcaatc agaagtttg 
ctcaacaat aaaggtgta gttagtagc tgcggtttg 
acttcttcc aatttacca gtaattact ctgacagat 
ggacgggcg tattcctac gctagtgat ctccaaaca 
tgggtacag ttatcgaat cctcttgat ccactaagt 
cgagtgggt ggaagtctc acaaggaga gtgtctcca 
actttacca ctggcctca ttacaacga gtgatgccc 
tttcatggt cctcacgat actctacct tcgggtgtt 
acgttgagt ttcttagag aatcagata actgcgagt 
tctattatt agttcgcat gatgaatac aggcattgg 
ttgattgcc accgtcaat tatttactt gataaagga 
cctgagtgt cggttgaga gtaattttg gatgagtta 
ttggggccc acatactcg tcaacagcg gcgaaaaaa 
tcgaaaaac agcgacaag attttgggg aaaccaagt 
cacgcgttt ctgcaagaa attttggaa ataatcaag 
acaaaattg ccatggcag cggctgtat aaggaatac 
agagagcag ttagatgaa attcttcac acaatgtaa
    mRNA sequence
    • >TC031054 RA
      tcgttttac caactcgct gggatatcc tgtataagc 
aaccagtaa aacacaatg ttatcaaaa tgaacgcca 
ctgtccaat ggccaacgc agggcgtac gccaatggt 
gatggcttg ctttgtttg acatttctg ttatttaag 
gttagaaaa gtttacacc gatcgtagt taatacatc 
aattctttt attaaacat gcttcaagt gtaaatcat 
agattaaca ctaccatta acgatcaag ttttgaagt 
gttcaataa acaagtaaa atgattttt ttcaaaccg 
tcttgggta actcacgaa gggagtaaa ccaattttc 
tcagtggat atccacccc aaaggcgga cgctttgcc 
accggaggc caaggtggt tccggcggc cgcatagta 
atctggaat attgctccc gttttgtcc gaaaatgac 
gaaagtagc ctgaaaatt ccgaaaatg ttgtgccag 
atggacaat cacttggcg tgtgtaaat gtggtgagg 
tggagcagt gagggtcat ttattggcc tcagggggt 
gatgataaa cttgtcatg atttggagg cttacaaac 
gagggtagt tcatcgatt tttggtagt ggtaaagtc 
aatgtggag acttggaag tgtgttcac actctcaat 
tcacacaat ggggatgtt ttggatctg gcttgggcc 
ccacacgat ggttggttg gcatctgga agtgtggat 
aataccgta ataatctgg aatgctcat aagtttcca 
gaaaaggtt gctgttttg aaaggacac accggcatg 
gtcaagggt gttacttgg gatcccgtg ggcaaatat 
atagctagt cagtccgat gataaatca ttgagaatt 
tggagaact tgcgattgg gttcagcag gaggtggtt 
aaagaccct tttgttgat tgttcagca accacacat 
gttttaaga ctatcatgg tcacctgat gggcaatac 
cttgtctct tcacatgcc atgaatgga ggaggacct 
actgctcaa attattgaa cgagaaggt tggagacaa 
gataaggat tttgttggt caccgaaaa gcagtcaca 
tgtgttaga ttcaattca aacatttta aaaaagatc 
tctgctggc agtcccaag agtcagcaa tactgttgc 
tgtgcaatt ggttcaagg gacaggtct atcagtgta 
tggttgacg tctctgaaa cgaccatta gtggtaatc 
aaagatctg tttgacgac agtgtatta gacatttcg 
tggagtagc aatggttta tatctattc gcatgttct 
tgggacgga agcgtagct tgcgttata ttcgaccag 
cgagaaatc ggaatcccg ttatcaatt aaagaaaaa 
aatgatctc tatgaacgt atttatcat aaatctctg 
caaaaaagt tggaattta agctttagt gggactcaa 
atcgtggaa aatcccgaa cttttaact gccattgac 
aaaattaac gaaagtagt aataacagt gttaatctt 
gagttaccc aaaccggcg tcgagagat gtttcgttt 
gagtcacca aaaccaaac accacgatt ttagtacct 
gctaataag caagtggaa acgagactt ccttccgga 
aaaagacgt ataactccc atgttgttg accaccgtt 
caaacaacc accgtggaa gcaacaaac agtcctcca 
aagacaaat ctagaagtg ccgacaaaa gaaagcaca 
tcttttagt agttcttcg ccaacaaag agtaaaatt 
gttgttgag actgtagta gatcaagtc aaacacccc 
acttttggt ggctcatca atgaaaagt tcgatcttg 
aatgatgca aataaattg ataagtgcg cctccagga 
acccaaatt aatcagtta acttgttgc gaagtgccg 
ggagatcag cctatagtc cagtttatt aatgtttta 
gatccactg aaactgcct tcaggtaca tcagtcacc 
aaacagtgc gggacctta aaaatacag ataaccaat 
aactgccac aaaacttcg aaaggatct ttatcgagt 
attgaagcg tataaaaat atcgatgat aaagaacca 
aactgggat acgtacatt ggttccacc attaggtgt 
ttagcagca aacagaaaa tttatcgtg gcatgttgt 
gaagacctt agtattaat tgttacgag atcaagtca 
ggagcacgt ccattacct ccaataatt atcgaagat 
gtcgtttct tcaattagt ttatcacaa gatagccac 
tgtttagta ttgacaaag actggtctt ttgtacatg 
tggaatttc gagctttgt aagagcatt ttaagtagg 
atatcaatc agaagtttg ctcaacaat aaaggtgta 
gttagtagc tgcggtttg acttcttcc aatttacca 
gtaattact ctgacagat ggacgggcg tattcctac 
gctagtgat ctccaaaca tgggtacag ttatcgaat 
cctcttgat ccactaagt cgagtgggt ggaagtctc 
acaaggaga gtgtctcca actttacca ctggcctca 
ttacaacga gtgatgccc tttcatggt cctcacgat 
actctacct tcgggtgtt acgttgagt ttcttagag 
aatcagata actgcgagt tctattatt agttcgcat 
gatgaatac aggcattgg ttgattgcc accgtcaat 
tatttactt gataaagga cctgagtgt cggttgaga 
gtaattttg gatgagtta ttggggccc acatactcg 
tcaacagcg gcgaaaaaa tcgaaaaac agcgacaag 
attttgggg aaaccaagt cacgcgttt ctgcaagaa 
attttggaa ataatcaag acaaaattg ccatggcag 
cggctgtat aaggaatac agagagcag ttagatgaa 
attcttcac acaatgtaa taacatttt ttttacatt 
ttataaaat aaaatatgc cataactac ttgtgccga 
cgtttcatt tcttcttca atgtattta tccaagtcg 
ctcaatggt ttgtagtag atgtggaca gtttgggcc 
cctgcaaac atagtgact gtcgctgcg gctaggaat 
gagaggtac ctagaccat gacacacct gccggcatt 
ttgctattg tgaggattc ttctaccaa aattttcgc 
ttgtctgcg tttgtacac tttatctgt taaacaatt 
attaatcat ttgattatt cgtttggtt taggaactt 
acagaaatt tgtttcacc gacttgcca gttgatcat 
agaaaactc taatgcggc tcctaggac gaaaaatag 
tggtaaaaa cctgtaaat tccgaaata cttttttcc 
aggccactt attcaaaag caactgaag agacacact 
tcgcttcat agtcaaaaa ttattacat aatagacac 
tacgtttta tattcgttg tacaacttg ttataatac 
caagggcct ttcgatgtt tttatgtct gataatggt 
aggactaat aatgacaaa tgttgatac aaatgccct 
tttcctgtg tttttcacg tgaaacgtc aaaaaaaca 
t