Status
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Gene information
General information
TC031054
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002637,TC001683
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16232596..16246493
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031054
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    hindwing irregular - Number of animals showing the phenotype: 4

    elytra not closed - Number of animals showing the phenotype: 4


    iBeetle screen iB_07039

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031054 RA
      MIFFKPSWV THEGSKPIF SVDIHPKGG RFATGGQGG 
      SGGRIVIWN IAPVLSEND ESSLKIPKM LCQMDNHLA 
      CVNVVRWSS EGHLLASGG DDKLVMIWR LTNEGSSSI 
      FGSGKVNVE TWKCVHTLN SHNGDVLDL AWAPHDGWL 
      ASGSVDNTV IIWNAHKFP EKVAVLKGH TGMVKGVTW 
      DPVGKYIAS QSDDKSLRI WRTCDWVQQ EVVKDPFVD 
      CSATTHVLR LSWSPDGQY LVSSHAMNG GGPTAQIIE 
      REGWRQDKD FVGHRKAVT CVRFNSNIL KKISAGSPK 
      SQQYCCCAI GSRDRSISV WLTSLKRPL VVIKDLFDD 
      SVLDISWSS NGLYLFACS WDGSVACVI FDQREIGIP 
      LSIKEKNDL YERIYHKSL QKSWNLSFS GTQIVENPE 
      LLTAIDKIN ESSNNSVNL ELPKPASRD VSFESPKPN 
      TTILVPANK QVETRLPSG KRRITPMLL TTVQTTTVE 
      ATNSPPKTN LEVPTKEST SFSSSSPTK SKIVVETVV 
      DQVKHPTFG GSSMKSSIL NDANKLISA PPGTQINQL 
      TCCEVPGDQ PIVQFINVL DPLKLPSGT SVTKQCGTL 
      KIQITNNCH KTSKGSLSS IEAYKNIDD KEPNWDTYI 
      GSTIRCLAA NRKFIVACC EDLSINCYE IKSGARPLP 
      PIIIEDVVS SISLSQDSH CLVLTKTGL LYMWNFELC 
      KSILSRISI RSLLNNKGV VSSCGLTSS NLPVITLTD 
      GRAYSYASD LQTWVQLSN PLDPLSRVG GSLTRRVSP 
      TLPLASLQR VMPFHGPHD TLPSGVTLS FLENQITAS 
      SIISSHDEY RHWLIATVN YLLDKGPEC RLRVILDEL 
      LGPTYSSTA AKKSKNSDK ILGKPSHAF LQEILEIIK 
      TKLPWQRLY KEYREQLDE ILHTM
    CDS sequence
    • >TC031054 RA
      atgattttt ttcaaaccg tcttgggta actcacgaa 
      gggagtaaa ccaattttc tcagtggat atccacccc 
      aaaggcgga cgctttgcc accggaggc caaggtggt 
      tccggcggc cgcatagta atctggaat attgctccc 
      gttttgtcc gaaaatgac gaaagtagc ctgaaaatt 
      ccgaaaatg ttgtgccag atggacaat cacttggcg 
      tgtgtaaat gtggtgagg tggagcagt gagggtcat 
      ttattggcc tcagggggt gatgataaa cttgtcatg 
      atttggagg cttacaaac gagggtagt tcatcgatt 
      tttggtagt ggtaaagtc aatgtggag acttggaag 
      tgtgttcac actctcaat tcacacaat ggggatgtt 
      ttggatctg gcttgggcc ccacacgat ggttggttg 
      gcatctgga agtgtggat aataccgta ataatctgg 
      aatgctcat aagtttcca gaaaaggtt gctgttttg 
      aaaggacac accggcatg gtcaagggt gttacttgg 
      gatcccgtg ggcaaatat atagctagt cagtccgat 
      gataaatca ttgagaatt tggagaact tgcgattgg 
      gttcagcag gaggtggtt aaagaccct tttgttgat 
      tgttcagca accacacat gttttaaga ctatcatgg 
      tcacctgat gggcaatac cttgtctct tcacatgcc 
      atgaatgga ggaggacct actgctcaa attattgaa 
      cgagaaggt tggagacaa gataaggat tttgttggt 
      caccgaaaa gcagtcaca tgtgttaga ttcaattca 
      aacatttta aaaaagatc tctgctggc agtcccaag 
      agtcagcaa tactgttgc tgtgcaatt ggttcaagg 
      gacaggtct atcagtgta tggttgacg tctctgaaa 
      cgaccatta gtggtaatc aaagatctg tttgacgac 
      agtgtatta gacatttcg tggagtagc aatggttta 
      tatctattc gcatgttct tgggacgga agcgtagct 
      tgcgttata ttcgaccag cgagaaatc ggaatcccg 
      ttatcaatt aaagaaaaa aatgatctc tatgaacgt 
      atttatcat aaatctctg caaaaaagt tggaattta 
      agctttagt gggactcaa atcgtggaa aatcccgaa 
      cttttaact gccattgac aaaattaac gaaagtagt 
      aataacagt gttaatctt gagttaccc aaaccggcg 
      tcgagagat gtttcgttt gagtcacca aaaccaaac 
      accacgatt ttagtacct gctaataag caagtggaa 
      acgagactt ccttccgga aaaagacgt ataactccc 
      atgttgttg accaccgtt caaacaacc accgtggaa 
      gcaacaaac agtcctcca aagacaaat ctagaagtg 
      ccgacaaaa gaaagcaca tcttttagt agttcttcg 
      ccaacaaag agtaaaatt gttgttgag actgtagta 
      gatcaagtc aaacacccc acttttggt ggctcatca 
      atgaaaagt tcgatcttg aatgatgca aataaattg 
      ataagtgcg cctccagga acccaaatt aatcagtta 
      acttgttgc gaagtgccg ggagatcag cctatagtc 
      cagtttatt aatgtttta gatccactg aaactgcct 
      tcaggtaca tcagtcacc aaacagtgc gggacctta 
      aaaatacag ataaccaat aactgccac aaaacttcg 
      aaaggatct ttatcgagt attgaagcg tataaaaat 
      atcgatgat aaagaacca aactgggat acgtacatt 
      ggttccacc attaggtgt ttagcagca aacagaaaa 
      tttatcgtg gcatgttgt gaagacctt agtattaat 
      tgttacgag atcaagtca ggagcacgt ccattacct 
      ccaataatt atcgaagat gtcgtttct tcaattagt 
      ttatcacaa gatagccac tgtttagta ttgacaaag 
      actggtctt ttgtacatg tggaatttc gagctttgt 
      aagagcatt ttaagtagg atatcaatc agaagtttg 
      ctcaacaat aaaggtgta gttagtagc tgcggtttg 
      acttcttcc aatttacca gtaattact ctgacagat 
      ggacgggcg tattcctac gctagtgat ctccaaaca 
      tgggtacag ttatcgaat cctcttgat ccactaagt 
      cgagtgggt ggaagtctc acaaggaga gtgtctcca 
      actttacca ctggcctca ttacaacga gtgatgccc 
      tttcatggt cctcacgat actctacct tcgggtgtt 
      acgttgagt ttcttagag aatcagata actgcgagt 
      tctattatt agttcgcat gatgaatac aggcattgg 
      ttgattgcc accgtcaat tatttactt gataaagga 
      cctgagtgt cggttgaga gtaattttg gatgagtta 
      ttggggccc acatactcg tcaacagcg gcgaaaaaa 
      tcgaaaaac agcgacaag attttgggg aaaccaagt 
      cacgcgttt ctgcaagaa attttggaa ataatcaag 
      acaaaattg ccatggcag cggctgtat aaggaatac 
      agagagcag ttagatgaa attcttcac acaatgtaa
    mRNA sequence
    • >TC031054 RA
      tcgttttac caactcgct gggatatcc tgtataagc 
      aaccagtaa aacacaatg ttatcaaaa tgaacgcca 
      ctgtccaat ggccaacgc agggcgtac gccaatggt 
      gatggcttg ctttgtttg acatttctg ttatttaag 
      gttagaaaa gtttacacc gatcgtagt taatacatc 
      aattctttt attaaacat gcttcaagt gtaaatcat 
      agattaaca ctaccatta acgatcaag ttttgaagt 
      gttcaataa acaagtaaa atgattttt ttcaaaccg 
      tcttgggta actcacgaa gggagtaaa ccaattttc 
      tcagtggat atccacccc aaaggcgga cgctttgcc 
      accggaggc caaggtggt tccggcggc cgcatagta 
      atctggaat attgctccc gttttgtcc gaaaatgac 
      gaaagtagc ctgaaaatt ccgaaaatg ttgtgccag 
      atggacaat cacttggcg tgtgtaaat gtggtgagg 
      tggagcagt gagggtcat ttattggcc tcagggggt 
      gatgataaa cttgtcatg atttggagg cttacaaac 
      gagggtagt tcatcgatt tttggtagt ggtaaagtc 
      aatgtggag acttggaag tgtgttcac actctcaat 
      tcacacaat ggggatgtt ttggatctg gcttgggcc 
      ccacacgat ggttggttg gcatctgga agtgtggat 
      aataccgta ataatctgg aatgctcat aagtttcca 
      gaaaaggtt gctgttttg aaaggacac accggcatg 
      gtcaagggt gttacttgg gatcccgtg ggcaaatat 
      atagctagt cagtccgat gataaatca ttgagaatt 
      tggagaact tgcgattgg gttcagcag gaggtggtt 
      aaagaccct tttgttgat tgttcagca accacacat 
      gttttaaga ctatcatgg tcacctgat gggcaatac 
      cttgtctct tcacatgcc atgaatgga ggaggacct 
      actgctcaa attattgaa cgagaaggt tggagacaa 
      gataaggat tttgttggt caccgaaaa gcagtcaca 
      tgtgttaga ttcaattca aacatttta aaaaagatc 
      tctgctggc agtcccaag agtcagcaa tactgttgc 
      tgtgcaatt ggttcaagg gacaggtct atcagtgta 
      tggttgacg tctctgaaa cgaccatta gtggtaatc 
      aaagatctg tttgacgac agtgtatta gacatttcg 
      tggagtagc aatggttta tatctattc gcatgttct 
      tgggacgga agcgtagct tgcgttata ttcgaccag 
      cgagaaatc ggaatcccg ttatcaatt aaagaaaaa 
      aatgatctc tatgaacgt atttatcat aaatctctg 
      caaaaaagt tggaattta agctttagt gggactcaa 
      atcgtggaa aatcccgaa cttttaact gccattgac 
      aaaattaac gaaagtagt aataacagt gttaatctt 
      gagttaccc aaaccggcg tcgagagat gtttcgttt 
      gagtcacca aaaccaaac accacgatt ttagtacct 
      gctaataag caagtggaa acgagactt ccttccgga 
      aaaagacgt ataactccc atgttgttg accaccgtt 
      caaacaacc accgtggaa gcaacaaac agtcctcca 
      aagacaaat ctagaagtg ccgacaaaa gaaagcaca 
      tcttttagt agttcttcg ccaacaaag agtaaaatt 
      gttgttgag actgtagta gatcaagtc aaacacccc 
      acttttggt ggctcatca atgaaaagt tcgatcttg 
      aatgatgca aataaattg ataagtgcg cctccagga 
      acccaaatt aatcagtta acttgttgc gaagtgccg 
      ggagatcag cctatagtc cagtttatt aatgtttta 
      gatccactg aaactgcct tcaggtaca tcagtcacc 
      aaacagtgc gggacctta aaaatacag ataaccaat 
      aactgccac aaaacttcg aaaggatct ttatcgagt 
      attgaagcg tataaaaat atcgatgat aaagaacca 
      aactgggat acgtacatt ggttccacc attaggtgt 
      ttagcagca aacagaaaa tttatcgtg gcatgttgt 
      gaagacctt agtattaat tgttacgag atcaagtca 
      ggagcacgt ccattacct ccaataatt atcgaagat 
      gtcgtttct tcaattagt ttatcacaa gatagccac 
      tgtttagta ttgacaaag actggtctt ttgtacatg 
      tggaatttc gagctttgt aagagcatt ttaagtagg 
      atatcaatc agaagtttg ctcaacaat aaaggtgta 
      gttagtagc tgcggtttg acttcttcc aatttacca 
      gtaattact ctgacagat ggacgggcg tattcctac 
      gctagtgat ctccaaaca tgggtacag ttatcgaat 
      cctcttgat ccactaagt cgagtgggt ggaagtctc 
      acaaggaga gtgtctcca actttacca ctggcctca 
      ttacaacga gtgatgccc tttcatggt cctcacgat 
      actctacct tcgggtgtt acgttgagt ttcttagag 
      aatcagata actgcgagt tctattatt agttcgcat 
      gatgaatac aggcattgg ttgattgcc accgtcaat 
      tatttactt gataaagga cctgagtgt cggttgaga 
      gtaattttg gatgagtta ttggggccc acatactcg 
      tcaacagcg gcgaaaaaa tcgaaaaac agcgacaag 
      attttgggg aaaccaagt cacgcgttt ctgcaagaa 
      attttggaa ataatcaag acaaaattg ccatggcag 
      cggctgtat aaggaatac agagagcag ttagatgaa 
      attcttcac acaatgtaa taacatttt ttttacatt 
      ttataaaat aaaatatgc cataactac ttgtgccga 
      cgtttcatt tcttcttca atgtattta tccaagtcg 
      ctcaatggt ttgtagtag atgtggaca gtttgggcc 
      cctgcaaac atagtgact gtcgctgcg gctaggaat 
      gagaggtac ctagaccat gacacacct gccggcatt 
      ttgctattg tgaggattc ttctaccaa aattttcgc 
      ttgtctgcg tttgtacac tttatctgt taaacaatt 
      attaatcat ttgattatt cgtttggtt taggaactt 
      acagaaatt tgtttcacc gacttgcca gttgatcat 
      agaaaactc taatgcggc tcctaggac gaaaaatag 
      tggtaaaaa cctgtaaat tccgaaata cttttttcc 
      aggccactt attcaaaag caactgaag agacacact 
      tcgcttcat agtcaaaaa ttattacat aatagacac 
      tacgtttta tattcgttg tacaacttg ttataatac 
      caagggcct ttcgatgtt tttatgtct gataatggt 
      aggactaat aatgacaaa tgttgatac aaatgccct 
      tttcctgtg tttttcacg tgaaacgtc aaaaaaaca 
      t
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