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Gene information
General information
TC001682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid529: 2566..5832
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001682
    iBeetle screen iB_00280
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001682 RA
      MKLIDKSID KEGQGYVTL VPEEPEDMW HAYNLISEG 
      DYVRSSTVR KVQSESSTG SSTSNRVRT MLTIAVETI 
      DFDTQACML RLKGKNVEE NQYVKMGAY HTLDLEMNR 
      KFRLTKHEW DSVSLERVE MACDPTKSA DLAAVIMQD 
      GLAQICLIT SSMTIVRAK IEASIPRKR KNFIQQHEK 
      ALAKFYDNI IQGILRHID FDIVKCVII ASPGFVRDQ 
      FYEYMFQMA VKTDNKTLL ENKSKFMLI HSSTGFKHS 
      LKEILQDPA VVNKISDTK AASEVKALE VFYTTLQCE 
      PDKAFYGKK HVEKANEAQ AIETLLISD NLFRCKDVN 
      LRKEYVKLV DSVRENGGD VKIFSSLHI SGEQLEQLT 
      GIAAILRFP MPELDEDDE SETED
    CDS sequence
    • >TC001682 RA
      atgaaactt attgacaaa tccattgat aaagaaggc 
      caagggtat gtgaccctt gtgcccgag gaacctgaa 
      gatatgtgg cacgcgtac aatctcata tcggaggga 
      gattacgtc cgcagtagc acagtccga aaagtgcag 
      tccgagtcg tcaacaggt tcttccaca tcaaaccgt 
      gtcagaacc atgctgacg atcgccgtt gaaacgatc 
      gactttgat actcaagca tgcatgcta agactgaag 
      gggaaaaac gtggaagag aaccaatac gtcaagatg 
      ggggcctac cacacgttg gacctcgaa atgaaccga 
      aagtttcgc ctgaccaag cacgagtgg gactccgtg 
      tccctcgag cgcgtggaa atggcctgc gacccgacc 
      aagagcgcc gacttggcc gctgtgata atgcaagat 
      gggttggca cagatctgc ttaataacg tccagcatg 
      accatagtt cgggccaaa atcgaggcg tccattccg 
      cgaaaacgc aaaaacttc atccaacag cacgaaaag 
      gcgttggcg aagttttac gacaatatt attcaaggc 
      atcttgagg cacattgac ttcgatatc gtcaagtgt 
      gtcataatt gcctcgcca gggttcgtc cgcgaccag 
      ttttacgaa tacatgttt caaatggct gttaaaacc 
      gataacaag acgcttttg gaaaataag tctaagttt 
      atgttgatt cactcgtct acagggttc aaacattcg 
      ttgaaagaa attctccaa gatccggct gtggttaat 
      aaaatttcc gacaccaaa gcggctagc gaagttaaa 
      gcacttgaa gttttctac acgacgctg caatgtgag 
      ccggataag gcattttat gggaaaaaa cacgtggag 
      aaggctaac gaggcacaa gcgattgag acacttctt 
      atatctgat aatctgttt aggtgtaaa gatgtaaat 
      ttacgtaaa gagtatgta aaattggta gactctgtg 
      agggaaaat ggaggcgat gttaagata ttctcaagt 
      ctgcatata tcaggagag caactagaa caattgacc 
      ggcattgca gcaattctc cgcttccca atgcccgaa 
      ctcgacgaa gacgatgaa agcgagacc gaagactaa
    mRNA sequence
    • >TC001682 RA
      gatcggcga aatcaaaat ggcgaaaat taacacgag 
      tgttgtcat gtatgtcct tgaaaaaag taattcttt 
      aaaaatgat gaattccca aaacatcgc atttttatc 
      gcatttagt gagttcgaa aatatcgtc aaaatgaaa 
      cttattgac aaatccatt gataaagaa ggccaaggg 
      tatgtgacc cttgtgccc gaggaacct gaagatatg 
      tggcacgcg tacaatctc atatcggag ggagattac 
      gtccgcagt agcacagtc cgaaaagtg cagtccgag 
      tcgtcaaca ggttcttcc acatcaaac cgtgtcaga 
      accatgctg acgatcgcc gttgaaacg atcgacttt 
      gatactcaa gcatgcatg ctaagactg aaggggaaa 
      aacgtggaa gagaaccaa tacgtcaag atgggggcc 
      taccacacg ttggacctc gaaatgaac cgaaagttt 
      cgcctgacc aagcacgag tgggactcc gtgtccctc 
      gagcgcgtg gaaatggcc tgcgacccg accaagagc 
      gccgacttg gccgctgtg ataatgcaa gatgggttg 
      gcacagatc tgcttaata acgtccagc atgaccata 
      gttcgggcc aaaatcgag gcgtccatt ccgcgaaaa 
      cgcaaaaac ttcatccaa cagcacgaa aaggcgttg 
      gcgaagttt tacgacaat attattcaa ggcatcttg 
      aggcacatt gacttcgat atcgtcaag tgtgtcata 
      attgcctcg ccagggttc gtccgcgac cagttttac 
      gaatacatg tttcaaatg gctgttaaa accgataac 
      aagacgctt ttggaaaat aagtctaag tttatgttg 
      attcactcg tctacaggg ttcaaacat tcgttgaaa 
      gaaattctc caagatccg gctgtggtt aataaaatt 
      tccgacacc aaagcggct agcgaagtt aaagcactt 
      gaagttttc tacacgacg ctgcaatgt gagccggat 
      aaggcattt tatgggaaa aaacacgtg gagaaggct 
      aacgaggca caagcgatt gagacactt cttatatct 
      gataatctg tttaggtgt aaagatgta aatttacgt 
      aaagagtat gtaaaattg gtagactct gtgagggaa 
      aatggaggc gatgttaag atattctca agtctgcat 
      atatcagga gagcaacta gaacaattg accggcatt 
      gcagcaatt ctccgcttc ccaatgccc gaactcgac 
      gaagacgat gaaagcgag accgaagac taatcatgt 
      caaaaacac tggttgtat tcgcgtttt taactcttt 
      gttgatcat ttttaaata aattaggga atttgttgt 
      atattttta attgttatg tgaataatt gctgtagat 
      tttt