| General information | |||
| TC001676 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 2603671..2634753 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001676 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00279 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC001676 RA
MGDTETRNR KYAEPSRDL LRRDQHVDG CDEVDLHPL SNQVGGHTR LMVLNPSTI CKPLNYREL DFYQNIQDQ DIKMFVPKY KGVMQATLC SGGKIEKRY SPSFRDESS RSKTDRKRK REDVLKMRI HHTGNPKDV LKSISQSDN TNKQYFLML ENITSHYTH PCILDLKMG TRQHGDDAS AEKRSKQMA KCAASTSAS LGVRLCGMQ VYQAASEIY YKKDKYWGR ELNEEGFKN ALCTFFDNG FGLRVSVIQ QVISKLEQL RRVIEKQSC YRFYSCSLL IVYEGNGGI PLLKPEPCL EPLFCESDC TEESSREVP CCYDADTSN SSADFNLSQ DEVSQDSHH RGFGEAAAR GAKYANFYP ISEETVFLD SPPTIPTIT TSSPLSIDS WMMYSNSSS DEYSNLNGM SSNEDTSDF EPCLKTSLQ FGPLELDDE DEELAATVS HKNATKRLR VKERAGRGA PANVDVRMI DFAHTSFIS TSDEGSSGV VHQGPDGGF LTGLDSLKR LLSQILAEG
- >TC001676 RB
MQATLCSGG KIEKRYSPS FRDESSRSK TDRKRKRED VLKMRIHHT GNPKDVLKS ISQSDNTNK QYFLMLENI TSHYTHPCI LDLKMGTRQ HGDDASAEK RSKQMAKCA ASTSASLGV RLCGMQVYQ AASEIYYKK DKYWGRELN EEGFKNALC TFFDNGFGL RVSVIQQVI SKLEQLRRV IEKQSCYRF YSCSLLIVY EGNGGIPLL KPEPCLEPL FCESDCTEE SSREVPCCY DADTSNSSA DFNLSQDEV SQDSHHRGF GEAAARGAK YANFYPISE ETVFLDSPP TIPTITTSS PLSIDSWMM YSNSSSDEY SNLNGMSSN EDTSDFEPC LKTSLQFGP LELDDEDEE LAATVSHKN ATKRLRVKE RAGRGAPAN VDVRMIDFA HTSFISTSD EGSSGVVHQ GPDGGFLTG LDSLKRLLS QILAEG
- >TC001676 RC
MTGVWEILK PATGNMRNH LGTSSDVTN MLMAVMKST YIHLAIKSE ATRVSWC
- >TC001676 RA
atgggagat actgaaacc cgcaaccgg aaatatgcg gaaccatct cgggacctc ctcagacgt gaccaacat gttgatggc tgtgatgaa gtcgactta catccactt agcaatcaa gtcggaggc cacacgcgt ctcatggtg ctgaacccg agcacgatc tgtaagccc ctgaattac agagagttg gatttttac cagaacatt caggaccaa gatatcaaa atgtttgta ccgaaatat aaaggggtt atgcaggcg acgctttgt agcggaggc aaaatcgaa aaacgctac agtcccagt tttcgagat gaatcttcc agaagtaag actgaccgc aagagaaag cgagaagat gttttgaaa atgaggatc caccacacg ggcaacccc aaagacgtc ctgaaaagc atctcgcaa tcagacaat acgaacaag caatacttc ctaatgtta gaaaatatc acgtcccac tacacgcat ccgtgcatc ctcgacctg aagatgggc actcgccag cacggcgac gacgcttcg gccgaaaaa cgttccaaa cagatggca aagtgcgcc gcaagcact tcggcatcg ttgggggtg cgtttgtgc ggaatgcaa gtgtatcaa gcggcgtcc gaaatctac tacaaaaag gacaaatat tggggaaga gagttgaac gaagaaggt ttcaaaaat gccttgtgt acgtttttc gacaacgga ttcggactt agagtctcc gtgatacaa caagtgatc tcaaaattg gagcagctg cgaagggtt atcgagaag caaagctgc tacaggttc tattcgtgc tctttactt atagtatac gaaggcaac ggtggcatt ccgctccta aagcccgaa ccctgcctg gagcccctc ttctgcgag agcgactgc actgaagaa agtagtcgg gaagtcccc tgttgctac gacgccgac accagcaac tcctctgcc gacttcaac ctgagccaa gacgaagtc tcgcaagac tcccaccat cgtggtttc ggcgaggcg gcggctcgc ggcgccaaa tacgccaac ttctacccc atttccgaa gagacagta ttcctggat tcgcccccg actataccc accatcacc acaagcagc cccctctcc atcgacagc tggatgatg tactcgaac agcagcagt gacgaatat tcgaacttg aacgggatg agctcgaac gaagacact tccgatttt gagccgtgt ctgaagaca agtcttcag ttcggccct ttggagctg gacgatgaa gatgaagag ttggctgcg acggtgtcg cacaagaac gcgaccaag aggttaagg gtgaaggaa agggcaggg aggggggcg ccggccaac gttgatgtg cgcatgatc gattttgcg cacacttcg tttatatcg acgagcgac gaagggtcg agtggcgtt gtgcaccaa gggccagat gggggtttc ctgacaggt ttggacagt ttaaagcgg ttattgtcg cagattttg gccgaaggg tga
- >TC001676 RB
atgcaggcg acgctttgt agcggaggc aaaatcgaa aaacgctac agtcccagt tttcgagat gaatcttcc agaagtaag actgaccgc aagagaaag cgagaagat gttttgaaa atgaggatc caccacacg ggcaacccc aaagacgtc ctgaaaagc atctcgcaa tcagacaat acgaacaag caatacttc ctaatgtta gaaaatatc acgtcccac tacacgcat ccgtgcatc ctcgacctg aagatgggc actcgccag cacggcgac gacgcttcg gccgaaaaa cgttccaaa cagatggca aagtgcgcc gcaagcact tcggcatcg ttgggggtg cgtttgtgc ggaatgcaa gtgtatcaa gcggcgtcc gaaatctac tacaaaaag gacaaatat tggggaaga gagttgaac gaagaaggt ttcaaaaat gccttgtgt acgtttttc gacaacgga ttcggactt agagtctcc gtgatacaa caagtgatc tcaaaattg gagcagctg cgaagggtt atcgagaag caaagctgc tacaggttc tattcgtgc tctttactt atagtatac gaaggcaac ggtggcatt ccgctccta aagcccgaa ccctgcctg gagcccctc ttctgcgag agcgactgc actgaagaa agtagtcgg gaagtcccc tgttgctac gacgccgac accagcaac tcctctgcc gacttcaac ctgagccaa gacgaagtc tcgcaagac tcccaccat cgtggtttc ggcgaggcg gcggctcgc ggcgccaaa tacgccaac ttctacccc atttccgaa gagacagta ttcctggat tcgcccccg actataccc accatcacc acaagcagc cccctctcc atcgacagc tggatgatg tactcgaac agcagcagt gacgaatat tcgaacttg aacgggatg agctcgaac gaagacact tccgatttt gagccgtgt ctgaagaca agtcttcag ttcggccct ttggagctg gacgatgaa gatgaagag ttggctgcg acggtgtcg cacaagaac gcgaccaag aggttaagg gtgaaggaa agggcaggg aggggggcg ccggccaac gttgatgtg cgcatgatc gattttgcg cacacttcg tttatatcg acgagcgac gaagggtcg agtggcgtt gtgcaccaa gggccagat gggggtttc ctgacaggt ttggacagt ttaaagcgg ttattgtcg cagattttg gccgaaggg tga
- >TC001676 RC
atgactggg gtatgggag atactgaaa cccgcaacc ggaaatatg cggaaccat ctcgggacc tcctcagac gtgaccaac atgttgatg gctgtgatg aagtcgact tacatccac ttagcaatc aagtcggag gccacacgc gtctcatgg tgctga
- >TC001676 RA
tgtttcacc aacattcaa taagaaagc gtttcgacc tcgcgttca gccagtttt cttcaaaca cagttcaac gaaggaccg catttcaca tttatttat ttatttttt taacaacac gacgtttgt attgtattt tgtacagga agtcttaaa aaaaacaaa aattcgatc atatgttga tgaggtgtt caattttcg tcagaggcc actttcaca attcgtcat gatcgtagc gggagtgaa acccgttgt aatgtgacc aatagttcg tttgcagtg tttctgtgt ttgtttttc tgttttctg ttgttgaaa agtgcagtg tgagaagaa ctgtgggcg tgatttcag ccccgaact gcatcgtaa tcgatagtg ttttcaagc aacgttgcc acgtcacgc aattgaact gacaacgtc gcacagctg accgggaca tcgtacttt gttatttgt tgcgaattt cctcggaga agaaaggac acttcagaa gtgtgtttg tgccaacaa gaaggggat ggtgtactt gttggatga ctggggtat gggagatac tgaaacccg caaccggaa atatgcgga accatctcg ggacctcct cagacgtga ccaacatgt tgatggctg tgatgaagt cgacttaca tccacttag caatcaagt cggaggcca cacgcgtct catggtgct gaacccgag cacgatctg taagcccct gaattacag agagttgga tttttacca gaacattca ggaccaaga tatcaaaat gtttgtacc gaaatataa aggggttat gcaggcgac gctttgtag cggaggcaa aatcgaaaa acgctacag tcccagttt tcgagatga atcttccag aagtaagac tgaccgcaa gagaaagcg agaagatgt tttgaaaat gaggatcca ccacacggg caaccccaa agacgtcct gaaaagcat ctcgcaatc agacaatac gaacaagca atacttcct aatgttaga aaatatcac gtcccacta cacgcatcc gtgcatcct cgacctgaa gatgggcac tcgccagca cggcgacga cgcttcggc cgaaaaacg ttccaaaca gatggcaaa gtgcgccgc aagcacttc ggcatcgtt gggggtgcg tttgtgcgg aatgcaagt gtatcaagc ggcgtccga aatctacta caaaaagga caaatattg gggaagaga gttgaacga agaaggttt caaaaatgc cttgtgtac gtttttcga caacggatt cggacttag agtctccgt gatacaaca agtgatctc aaaattgga gcagctgcg aagggttat cgagaagca aagctgcta caggttcta ttcgtgctc tttacttat agtatacga aggcaacgg tggcattcc gctcctaaa gcccgaacc ctgcctgga gcccctctt ctgcgagag cgactgcac tgaagaaag tagtcggga agtcccctg ttgctacga cgccgacac cagcaactc ctctgccga cttcaacct gagccaaga cgaagtctc gcaagactc ccaccatcg tggtttcgg cgaggcggc ggctcgcgg cgccaaata cgccaactt ctaccccat ttccgaaga gacagtatt cctggattc gcccccgac tatacccac catcaccac aagcagccc cctctccat cgacagctg gatgatgta ctcgaacag cagcagtga cgaatattc gaacttgaa cgggatgag ctcgaacga agacacttc cgattttga gccgtgtct gaagacaag tcttcagtt cggcccttt ggagctgga cgatgaaga tgaagagtt ggctgcgac ggtgtcgca caagaacgc gaccaagag gttaagggt gaaggaaag ggcagggag gggggcgcc ggccaacgt tgatgtgcg catgatcga ttttgcgca cacttcgtt tatatcgac gagcgacga agggtcgag tggcgttgt gcaccaagg gccagatgg gggtttcct gacaggttt ggacagttt aaagcggtt attgtcgca gattttggc cgaagggtg agataaaga atcatcgca atgctctgt atttaacaa taaaatttt aaacatcaa aattttgat ggttattta gcctggtgt ttagacttt ccagatggt gtaaatagg acgtttagt atttgttac gttttaatt tataacagt attcttgta caaaattgt aggataaaa ttgaatgga tattttcat attccctag aggtatgaa agtttttca ttttctctt ttttcattt tattctgtg attcaactt gttttattt tttatggca gagtgggtg agtggatga gcaatttct agacattcc agaatttgt gtagagtta acttaacac gcttgtatt caaaatgaa tttcagaaa ttcatccat gttgaacat gacgctgtt gtaaaaaat gaatttgtt tcatattgt tgctcttat aattcccaa agtgggacc attgggtat atttgaatg tttctgatg ttatggttt ttacaaatt acgggatat gtttaggaa tttgagcga attgtgatg ttttgtgtt caaacttgt actattgtt aatttaaaa ataaaacac aagttgcat a
- >TC001676 RB
acgcttttc ataaggtat tacgtacaa aatttaaaa tttatttat taatttaag ggccgataa tgtgttggg acaccctat gtatgattt ttttgtatt tctctttgt ttttgtcgc attttgcga atcttgcca cctctggtg ccactctct cctgtgtga gtgagcgaa tggcgaatg cttggagcg atctggtta tcccaaaaa tcgtttact tgttttatt gagggaacc gccccctgg tggactggc tgacagaca gacggctgc cgctgcctg ccccacctc tcgaatgcc acttcgtcc attctgcga gggtttgta tggacgtcc ttcacgata aaagggttg gaagcccat tttatatac tatttttaa tcaagcaaa tagaatcca gattcgctt cataatcgc gatctgaat tctattccc aatactctt tgattcagt cagtactta aagtaaacg aacgctctg ttttttgtt ttgttgtca atttttacg tttctcatc acattatta ttcaacaca caatgtagc gtctgatta tttttctag tggcatttt tgctacact actaattcg tttgtacta aaatattac taaaaccta agtcggaat tatataagt ttaaggctt tggggggtt atgcaggcg acgctttgt agcggaggc aaaatcgaa aaacgctac agtcccagt tttcgagat gaatcttcc agaagtaag actgaccgc aagagaaag cgagaagat gttttgaaa atgaggatc caccacacg ggcaacccc aaagacgtc ctgaaaagc atctcgcaa tcagacaat acgaacaag caatacttc ctaatgtta gaaaatatc acgtcccac tacacgcat ccgtgcatc ctcgacctg aagatgggc actcgccag cacggcgac gacgcttcg gccgaaaaa cgttccaaa cagatggca aagtgcgcc gcaagcact tcggcatcg ttgggggtg cgtttgtgc ggaatgcaa gtgtatcaa gcggcgtcc gaaatctac tacaaaaag gacaaatat tggggaaga gagttgaac gaagaaggt ttcaaaaat gccttgtgt acgtttttc gacaacgga ttcggactt agagtctcc gtgatacaa caagtgatc tcaaaattg gagcagctg cgaagggtt atcgagaag caaagctgc tacaggttc tattcgtgc tctttactt atagtatac gaaggcaac ggtggcatt ccgctccta aagcccgaa ccctgcctg gagcccctc ttctgcgag agcgactgc actgaagaa agtagtcgg gaagtcccc tgttgctac gacgccgac accagcaac tcctctgcc gacttcaac ctgagccaa gacgaagtc tcgcaagac tcccaccat cgtggtttc ggcgaggcg gcggctcgc ggcgccaaa tacgccaac ttctacccc atttccgaa gagacagta ttcctggat tcgcccccg actataccc accatcacc acaagcagc cccctctcc atcgacagc tggatgatg tactcgaac agcagcagt gacgaatat tcgaacttg aacgggatg agctcgaac gaagacact tccgatttt gagccgtgt ctgaagaca agtcttcag ttcggccct ttggagctg gacgatgaa gatgaagag ttggctgcg acggtgtcg cacaagaac gcgaccaag aggttaagg gtgaaggaa agggcaggg aggggggcg ccggccaac gttgatgtg cgcatgatc gattttgcg cacacttcg tttatatcg acgagcgac gaagggtcg agtggcgtt gtgcaccaa gggccagat gggggtttc ctgacaggt ttggacagt ttaaagcgg ttattgtcg cagattttg gccgaaggg tgagataaa gaatcatcg caatgctct gtatttaac aataaaatt ttaaacatc aaaattttg atggttatt tagcctggt gtttagact ttccagatg gtgtaaata ggacgttta gtatttgtt acgttttaa tttataaca gtattcttg tacaaaatt gtaggataa aattgaatg gatattttc atattccct agaggtatg aaagttttt cattttctc ttttttcat tttattctg tgattcaac ttgttttat tttttatgg cagagtggg tgagtggat gagcaattt ctagacatt ccagaattt gtgtagagt taacttaac acgcttgta ttcaaaatg aatttcaga aattcatcc atgttgaac atgacgctg ttgtaaaaa atgaatttg tttcatatt gttgctctt ataattccc aaagtggga ccattgggt atatttgaa tgtttctga tgttatggt ttttacaaa ttacgggat atgtttagg aatttgagc gaattgtga tgttttgtg ttcaaactt gtactattg ttaatttaa aaataaaac acaagttgc ata
- >TC001676 RC
tgtttcacc aacattcaa taagaaagc gtttcgacc tcgcgttca gccagtttt cttcaaaca cagttcaac gaaggaccg catttcaca tttatttat ttatttttt taacaacac gacgtttgt attgtattt tgtacagga agtcttaaa aaaaacaaa aattcgatc atatgttga tgaggtgtt caattttcg tcagaggcc actttcaca attcgtcat gatcgtagc gggagtgaa acccgttgt aatgtgacc aatagttcg tttgcagtg tttctgtgt ttgtttttc tgttttctg ttgttgaaa agtgcagtg tgagaagaa ctgtgggcg tgatttcag ccccgaact gcatcgtaa tcgatagtg ttttcaagc aacgttgcc acgtcacgc aattgaact gacaacgtc gcacagctg accgggaca tcgtacttt gttatttgt tgcgaattt cctcggaga agaaaggac acttcagaa gtgtgtttg tgccaacaa gaaggggat ggtgtactt gttggatga ctggggtat gggagatac tgaaacccg caaccggaa atatgcgga accatctcg ggacctcct cagacgtga ccaacatgt tgatggctg tgatgaagt cgacttaca tccacttag caatcaagt cggaggcca cacgcgtct catggtgct gaacccgag cacgatctg taagcccct gaattacag agagttgga tttttacca gaacattca ggaccaaga tatcaaaat gtttgtacc gaaatataa ag
