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Gene information
General information
TC001670 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2291366..2303457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001670
      iBeetle screen iB_00278

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      remarks: animals lost,
      Adult morphology
      remarks: animals lost,
      Fertility
      egg production: number <50% of expected -
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1

      ovary without detectable defects - Number of animals showing the phenotype: 3
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001670 RA
        MSGGPRART SPLRATVPL SCVTKGGKD APKGAAIRR 
TASLDAIHR RLAARPAPS AVPTHTDRA SSLDSLQAL 
VDAVEDKGA KVGPKTRRD DKGAQSRNL PSPFAKSSV 
VVPPKSSRN SEEGLNQEL ELYANVETL ECDRLQEPI 
PEGRKAPVA ELIREGPYS RIYQPTNPN SSEDSQGSS 
PDQERILSG SSPQINRFS SSAPPDGCD RVLSKSVPI 
LRPIPREIV VSSHPNTSF TLKPSSYSA FESLQTSKD 
SSDDSPKVP EQEDP
      CDS sequence
      • >TC001670 RA
        atgtcgggc ggtccccgg gccaggact tcgcccctg 
agggccacc gtgcccttg tcgtgcgtc accaaaggt 
ggaaaagac gcccccaag ggggctgct atccggcgg 
acggcgtcg ctggacgcc atacacaga cgcttggcg 
gcgaggcct gcccccagc gccgtcccc acgcacacc 
gacagggcc agcagtttg gactcgttg caggctttg 
gtcgatgcg gtggaagac aagggggcg aaagtgggg 
cccaagact aggagggat gacaagggg gcacaaagt 
aggaacctt ccgtctccg tttgcgaaa agttcagtg 
gttgttcca ccgaaatcg tcgcgaaat tccgaagaa 
ggtctcaat caagaattg gaactttat gccaatgtg 
gaaacactc gaatgtgat agattacaa gaaccgata 
cctgaagga cgaaaagct ccagtagct gaactgata 
agagaagga ccatattcg agaatatat caaccaacc 
aatccgaat tcatcagaa gacagtcaa ggttcgagt 
cccgatcaa gagcgcatt ctgtcaggt tcctcgccc 
caaatcaac cgtttttca tcaagtgcg ccccctgac 
ggctgcgac cgcgtgcta tcgaaatca gtacctatc 
ctacgaccg atacctcgg gaaatcgtc gtttcgtcg 
catcccaac acctcattc acgctcaaa ccaagctca 
tattcggct tttgagagt ctccaaaca tccaaagac 
tcctcggac gattcacca aaagtgccc gaacaagag 
gacccatag
      mRNA sequence
      • >TC001670 RA
        tccgatcag ctgattttg acattcgtg acatcacca 
aaactccac cactgtcga attttcctc cctgaaaag 
cgacctgcg cactcctca actctcagc agacaaaag 
ctccctcaa caatgctaa gaaaatcag cgaaaaaca 
caattccgg tgttccggc gcaataccc gtcattgaa 
cgaacacac agagacagt gccgagatg tcgggcggt 
ccccgggcc aggacttcg cccctgagg gccaccgtg 
cccttgtcg tgcgtcacc aaaggtgga aaagacgcc 
cccaagggg gctgctatc cggcggacg gcgtcgctg 
gacgccata cacagacgc ttggcggcg aggcctgcc 
cccagcgcc gtccccacg cacaccgac agggccagc 
agtttggac tcgttgcag gctttggtc gatgcggtg 
gaagacaag ggggcgaaa gtggggccc aagactagg 
agggatgac aagggggca caaagtagg aaccttccg 
tctccgttt gcgaaaagt tcagtggtt gttccaccg 
aaatcgtcg cgaaattcc gaagaaggt ctcaatcaa 
gaattggaa ctttatgcc aatgtggaa acactcgaa 
tgtgataga ttacaagaa ccgatacct gaaggacga 
aaagctcca gtagctgaa ctgataaga gaaggacca 
tattcgaga atatatcaa ccaaccaat ccgaattca 
tcagaagac agtcaaggt tcgagtccc gatcaagag 
cgcattctg tcaggttcc tcgccccaa atcaaccgt 
ttttcatca agtgcgccc cctgacggc tgcgaccgc 
gtgctatcg aaatcagta cctatccta cgaccgata 
cctcgggaa atcgtcgtt tcgtcgcat cccaacacc 
tcattcacg ctcaaacca agctcatat tcggctttt 
gagagtctc caaacatcc aaagactcc tcggacgat 
tcaccaaaa gtgcccgaa caagaggac ccatagaca 
ttttcgagt ttcagatcg ttgcgaact tgacgcgac 
tgtctttta acacagtcg agtaatttt tatttaatt 
gtgtagaat ctcgctttt ttagttgtc gtctcaaac 
aaaggctgt ggacatgaa gtgtttttg ccaaaattg 
actgataat tgtttaatt ggaaacgat ctgaaattc 
agttcacag caacacatt ccagagtga actgtttaa 
taaaacgct gtgattctt tttataata ataataatg 
gcagaaatg atttgatca aagttgttt ttatgtaga 
aattttcta acctagaat tgattgatt gttaccttt 
cttgttact tgacgcatt ctcgtgtat gtacaaaca 
tatctaata aacagactg acagcat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY