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Gene information
General information
TC001668 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2407695..2418393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001668
      iBeetle screen iB_00277

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001668 RA
        MHVINYFII YWKPLAWSL LLLLVTCLS QFFTLPISG 
        LYLLGYVVT VAISTTWVS FVTVQNFLH QNKPLNLAK 
        LLNFKVKQF DDHFNNAAS IKQLTLDID KYFIEKWFV 
        YISKDPTFS DESRVILEE ILRKIINVQ LNLDNEVIV 
        QGVLNLYLK HLKEFRRSL KRRDKYSGK ISDLYRYSH 
        MCSASKKAK SYFIHQLTI NLLNHFINW ELGNSLPYQ 
        VLVSIISKK VMTYILKLL SNPEFINYY ILKSCAPEN 
        KIQELNLNS YNRVKITKI TEKGDTTPS DLVKSMSKT 
        NLAKVDTIE PQTKEIVEF KTEDVKIYE PKSCKTWYD 
        SLDLTSIPL GQDILDSIS LGESDKDVD NTELEKEEV 
        LSPISSAKN LLDEMKQMT NFEGLKTSM KPISDATAT 
        TIHNIKDFQ ESTVNNVVK PVSQVTSTA LHRIGGLHL 
        QDEAAGVVE GILDFGRTR IRKGLRLTG LQDSPPEEP 
        IKPPKKQIV KMDKIDDFE TTWINPVQE EQPKPETVT 
        KVPSIQTHE EISPEPEYE EAADLATSI AKLRSLLEQ 
        KSSESSLST PAVSPMPQD DISNKILLE DGDETDGVM 
        PSFYKFCAK TATGVIHNT LNTIKTALP GNVAECDRY 
        DGLEKWLFV EANTTDCDL YERMTKLLG ERKVFCTVD 
        TAYEALDSL ESLQQTDKL RQSNIQFDD ELDDFEVKL 
        PITKTLFDI LCELLADNK ETSLTQEPV IKSILLLFG 
        NYIEETVVT QANYYLRYC CANAKIPSE GKIETLSME 
        LEEFVTVVL SGIPNSFKC LVGENTVKD VINLLVSSL 
        QIEKINQDI MLQVFEILC MKLMEGSKQ LSPMISA
      CDS sequence
      • >TC001668 RA
        atgcatgtc atcaactat ttcataata tattggaaa 
        ccattggct tggtctctg ctcctctta ctcgtgaca 
        tgtttgagc cagtttttc acgttgccc ataagtggt 
        ctttatttg ctgggctat gtcgtaaca gtcgcaata 
        tcgacaact tgggtgagc tttgtgaca gtacagaat 
        tttttgcac cagaacaaa ccgctaaat ttggcaaaa 
        ctcctcaat tttaaagta aaacaattt gacgatcat 
        ttcaacaat gcggcctca atcaagcaa ctaacttta 
        gatatcgac aagtatttt atcgaaaag tggtttgtt 
        tacatcagt aaagacccg actttcagt gatgagagt 
        cgggtcatt ctcgaggag atcctccgg aaaattatc 
        aatgtccag ttgaatctt gataacgaa gtgatcgtt 
        caaggggtt ctcaatttg tatttgaaa cacttgaaa 
        gagtttcgg cggagtttg aaacgcagg gataaatat 
        tcggggaag atcagcgat ctgtacaga tactcacac 
        atgtgcagt gcttcaaag aaagcaaaa agttatttc 
        attcatcag ctcaccatt aatttattg aaccatttc 
        atcaactgg gaattggga aattcacta ccgtaccaa 
        gttctagtc tctattatt tcaaaaaaa gtcatgacg 
        tacattttg aaattgcta tcgaaccca gaatttata 
        aattattac attttgaaa agttgtgcg ccagaaaac 
        aagatacag gaactgaac ttgaacagt tataatcga 
        gtcaaaatt actaaaatt acggaaaaa ggtgacacc 
        acacctagc gatctagtc aagtcaatg agcaaaaca 
        aacttggcc aaagttgac acaattgag cctcagacc 
        aaagaaata gttgaattt aaaactgaa gatgtaaag 
        atttatgag cccaaaagt tgcaaaacg tggtacgat 
        tcgttagac ttgacttca attcctttg gggcaagat 
        atactcgat agtatcagt ctgggagag tcggataag 
        gatgttgat aatacagaa ttggaaaag gaggaggtt 
        ttgtctccg atttcttca gcaaagaat cttcttgac 
        gaaatgaag caaatgacc aattttgag ggtttgaag 
        acttccatg aaaccgata agtgacgcc acggctact 
        acgattcat aatatcaaa gattttcaa gaaagtaca 
        gtaaataat gttgttaag cctgtgtca caggtcact 
        tcgactgca ttacacaga attggtggc ttacactta 
        caggacgag gctgcagga gtcgtcgaa ggaattttg 
        gattttggt agaacccga atacgtaaa ggccttcgt 
        cttaccggt cttcaagac agcccccct gaggagccc 
        attaaaccc ccaaaaaag caaattgtc aaaatggac 
        aaaatcgat gactttgaa acaacttgg atcaatcca 
        gtacaagag gaacaacca aaacctgaa actgtaacc 
        aaagtccct tcgatacaa actcacgaa gaaataagt 
        ccagaacct gaatatgaa gaagccgct gatttagcg 
        acaagtatc gcaaaattg cgatctttg ctcgagcaa 
        aagtcgtct gaatcgagt ttatcgaca cctgccgtc 
        agtccgatg ccacaggac gatatttct aataaaatt 
        ttgctagaa gacggtgac gagaccgac ggtgtaatg 
        ccgagtttt tataaattt tgtgcgaaa actgcaact 
        ggagtcatt cataatacg ctaaatacg attaaaacc 
        gctcttccg ggaaatgtg gcagagtgt gacagatat 
        gatggtctg gagaagtgg ctgtttgtt gaggcgaac 
        acgacggat tgcgatttg tacgaacgt atgactaaa 
        cttctcggc gaaaggaaa gttttctgc acagtagac 
        acggcctat gaagctctc gattcctta gaatctctc 
        caacaaacc gacaaactt cgtcaatct aatatccaa 
        ttcgacgat gaacttgac gattttgaa gtcaaatta 
        ccgattact aaaaccctt tttgacatt ttatgcgaa 
        cttttagca gacaacaaa gaaacgtct ctgacccag 
        gaaccagtc atcaaatct attttatta ctttttgga 
        aattatatt gaagaaact gttgtaaca caagctaac 
        tattatcta cgttattgt tgtgctaac gctaaaata 
        ccttcagag ggtaaaatt gagacgctt tctatggaa 
        ctagaagag tttgttaca gttgttttg agcggtatt 
        ccaaattct tttaaatgt ctagtaggg gaaaataca 
        gtaaaagat gttataaat ttgttggtt tcttccttg 
        caaatcgag aagatcaat caggacatc atgttgcaa 
        gtttttgaa atactttgc atgaaatta atggaaggg 
        agtaaacag ctatcgccg atgatttca gcttaa
      mRNA sequence
      • >TC001668 RA
        aaataagtg aggttatgt ctgtaaaca aaatgtaaa 
        taaaacgct ccaaatcag taaattcac cacaaaatc 
        agtattatt tcacctcgg tgatcaccc cacgactca 
        tttaaacca aaccaacgc gaatgtgta tgtcgtcac 
        cacgcgtcc ccccattta tggcatcgt attaaaatt 
        ctggtgaca aaatgtgtg agtagataa ggaaacaat 
        gtaatcaga agaacaaca gtaagtgtc ccacaatgc 
        atgtcatca actatttca taatatatt ggaaaccat 
        tggcttggt ctctgctcc tcttactcg tgacatgtt 
        tgagccagt ttttcacgt tgcccataa gtggtcttt 
        atttgctgg gctatgtcg taacagtcg caatatcga 
        caacttggg tgagctttg tgacagtac agaattttt 
        tgcaccaga acaaaccgc taaatttgg caaaactcc 
        tcaatttta aagtaaaac aatttgacg atcatttca 
        acaatgcgg cctcaatca agcaactaa ctttagata 
        tcgacaagt attttatcg aaaagtggt ttgtttaca 
        tcagtaaag acccgactt tcagtgatg agagtcggg 
        tcattctcg aggagatcc tccggaaaa ttatcaatg 
        tccagttga atcttgata acgaagtga tcgttcaag 
        gggttctca atttgtatt tgaaacact tgaaagagt 
        ttcggcgga gtttgaaac gcagggata aatattcgg 
        ggaagatca gcgatctgt acagatact cacacatgt 
        gcagtgctt caaagaaag caaaaagtt atttcattc 
        atcagctca ccattaatt tattgaacc atttcatca 
        actgggaat tgggaaatt cactaccgt accaagttc 
        tagtctcta ttatttcaa aaaaagtca tgacgtaca 
        ttttgaaat tgctatcga acccagaat ttataaatt 
        attacattt tgaaaagtt gtgcgccag aaaacaaga 
        tacaggaac tgaacttga acagttata atcgagtca 
        aaattacta aaattacgg aaaaaggtg acaccacac 
        ctagcgatc tagtcaagt caatgagca aaacaaact 
        tggccaaag ttgacacaa ttgagcctc agaccaaag 
        aaatagttg aatttaaaa ctgaagatg taaagattt 
        atgagccca aaagttgca aaacgtggt acgattcgt 
        tagacttga cttcaattc ctttggggc aagatatac 
        tcgatagta tcagtctgg gagagtcgg ataaggatg 
        ttgataata cagaattgg aaaaggagg aggttttgt 
        ctccgattt cttcagcaa agaatcttc ttgacgaaa 
        tgaagcaaa tgaccaatt ttgagggtt tgaagactt 
        ccatgaaac cgataagtg acgccacgg ctactacga 
        ttcataata tcaaagatt ttcaagaaa gtacagtaa 
        ataatgttg ttaagcctg tgtcacagg tcacttcga 
        ctgcattac acagaattg gtggcttac acttacagg 
        acgaggctg caggagtcg tcgaaggaa ttttggatt 
        ttggtagaa cccgaatac gtaaaggcc ttcgtctta 
        ccggtcttc aagacagcc cccctgagg agcccatta 
        aacccccaa aaaagcaaa ttgtcaaaa tggacaaaa 
        tcgatgact ttgaaacaa cttggatca atccagtac 
        aagaggaac aaccaaaac ctgaaactg taaccaaag 
        tcccttcga tacaaactc acgaagaaa taagtccag 
        aacctgaat atgaagaag ccgctgatt tagcgacaa 
        gtatcgcaa aattgcgat ctttgctcg agcaaaagt 
        cgtctgaat cgagtttat cgacacctg ccgtcagtc 
        cgatgccac aggacgata tttctaata aaattttgc 
        tagaagacg gtgacgaga ccgacggtg taatgccga 
        gtttttata aattttgtg cgaaaactg caactggag 
        tcattcata atacgctaa atacgatta aaaccgctc 
        ttccgggaa atgtggcag agtgtgaca gatatgatg 
        gtctggaga agtggctgt ttgttgagg cgaacacga 
        cggattgcg atttgtacg aacgtatga ctaaacttc 
        tcggcgaaa ggaaagttt tctgcacag tagacacgg 
        cctatgaag ctctcgatt ccttagaat ctctccaac 
        aaaccgaca aacttcgtc aatctaata tccaattcg 
        acgatgaac ttgacgatt ttgaagtca aattaccga 
        ttactaaaa ccctttttg acattttat gcgaacttt 
        tagcagaca acaaagaaa cgtctctga cccaggaac 
        cagtcatca aatctattt tattacttt ttggaaatt 
        atattgaag aaactgttg taacacaag ctaactatt 
        atctacgtt attgttgtg ctaacgcta aaatacctt 
        cagagggta aaattgaga cgctttcta tggaactag 
        aagagtttg ttacagttg ttttgagcg gtattccaa 
        attctttta aatgtctag taggggaaa atacagtaa 
        aagatgtta taaatttgt tggtttctt ccttgcaaa 
        tcgagaaga tcaatcagg acatcatgt tgcaagttt 
        ttgaaatac tttgcatga aattaatgg aagggagta 
        aacagctat cgccgatga tttcagctt aattgtatt 
        aatgttgtt acgctttga atagatttt atgtcaatg 
        tttaacaaa tgtagaaaa taaaaagac acgttttat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY