Status
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Gene information
General information
TC001634 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1233836..1237517
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001634
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001634 RA
      MAYAYWKSG KTKDYAVFD LFFRKNPFQ GEFTIFAGL 
      EECLKFLEK FHYSISDIE YLKQTLPPN IEEEFYEFL 
      SSVSAKDVT LYAIAEGSV AFPRVPLMR VEGPLIIVQ 
      LLETTLLTL VNYASLMAT NAARYRMAA GNMKLFEFG 
      LRRAQGPDG GLSASKYSY IGGFDGTSN VLAGKLFNI 
      PVKGTHAHA YITSFNGFT DLRNTMLEP KDGAEPRDL 
      LQLALSWRT KLVPIFKVV ASEASDGEL AALISFAIA 
      FPDGFMALV DTYDVKRYP SHMNTTSVW SVTLPNTQS 
      HNEYRSGLL NFCSVALAL NDLGYRAVG IRLDSGDLA 
      YLSNVARQF FCEISDKFN IPWFEDLMI MASNDINEE 
      TILSLNEQG HKINCFGIG THLVTCQRQ PALGCVYKL 
      VELNGQPRI KLSQDVGKV TMPGRKDAY RLYSESGHA 
      LVDLLQRST EPPPAINEK VLCRHPFIE SKRAYVIPS 
      HVEKLLTLH WKNGAICQP LPNLQQIKE RVQASLRTL 
      RPDIKRNLN PTPFKVAVS DALYTFLHD LWLENAPIG 
      ELS
    CDS sequence
    • >TC001634 RA
      atggcttat gcatactgg aagtcgggg aagacaaag 
      gattacgcc gtgtttgac ttgttcttc cgcaaaaac 
      ccttttcaa ggggagttc acgattttc gcgggtttg 
      gaggaatgt ttgaaattt ttggaaaag tttcactac 
      tcgatcagc gatattgag tatttgaaa cagactctt 
      ccgccgaat attgaagaa gagttttac gagttcttg 
      agttcggtg tccgcgaag gatgtcact ttgtatgct 
      attgcggaa ggatcagtc gcatttcct agagtgcca 
      cttatgcga gttgaaggt cctttaatc atagtgcaa 
      ttgcttgaa acgacactg ctgactttg gttaattat 
      gcaagtctg atggcgaca aatgctgct aggtaccgg 
      atggcggca ggaaacatg aagttgttc gagtttgga 
      ctgcgccgg gcgcagggc cccgatggt gggctttca 
      gcgtcaaaa tattcttac atagggggc ttcgacggc 
      accagcaac gtattagcc gggaaactg ttcaacatc 
      ccggtgaag ggaacccac gcccatgcc tacatcacg 
      tcttttaac ggcttcacc gacttgcgg aacaccatg 
      ctggagccc aaagacggc gccgagccg cgggacctc 
      ctccagctg gccctctcc tggaggacc aagctggtt 
      ccgatcttc aaggttgtg gcttccgaa gcgagcgac 
      ggcgaactg gccgccctt atttcgttc gctattgcc 
      ttccctgac ggatttatg gccttagtt gacacgtat 
      gacgttaag aggtatccc tctcacatg aataccacc 
      tccgtgtgg tctgtcact ctgcccaac acccaatcg 
      cataacgag tacaggagt ggccttctc aacttttgc 
      tcagtagcg ctagctcta aacgatttg ggatatcgg 
      gctgtgggt atccgttta gatagtggg gacttggct 
      tatctatcg aatgtcgct cgccagttt ttctgcgag 
      attagtgac aagtttaac attccctgg tttgaggac 
      ttgatgata atggcttcc aacgacatc aacgaggag 
      acaatcctg agtctgaac gagcaaggg cacaaaatc 
      aattgtttc gggataggc acccatcta gtgacttgt 
      cagagacaa ccagccctg ggctgtgtc tacaagctg 
      gtcgagctg aacggccaa cccagaata aaacttagc 
      caggacgtc gggaaagtc acaatgcct ggacgcaaa 
      gacgcctat cgcctatac agcgaaagt gggcatgcc 
      ctcgttgat ctgctacaa agatcaacg gaaccgccc 
      ccagccatc aacgaaaaa gtcttatgc cgccatccg 
      ttcatcgag tccaaacgg gcatacgtt ataccatca 
      cacgtcgaa aaacttcta actcttcac tggaaaaac 
      ggcgccatt tgtcagcct ttacccaat cttcagcaa 
      ataaaagaa agagtgcag gcatctcta aggacctta 
      agacccgac attaagaga aaccttaat cctactcct 
      ttcaaggtt gcggtcagt gacgcgctc tacactttc 
      cttcacgac ttgtggctt gaaaacgcc ccaatcgga 
      gaattatcc taa
    mRNA sequence
    • >TC001634 RA
      cggtgcaaa acaagtccg atttgtgta caatttaaa 
      ttagttttc gcattgcaa atgataatt tttgtagta 
      aaacgccaa tctacagga gtttaatgc tatgatgcc 
      cttgcattg acctccgca ttgatcaat attgctact 
      caacacgct tgtacacta aaactaaat aatatttga 
      tcgacgagt gtaagattt gagcggttt acaatttta 
      gtagcgcac acgaactca acttgtaca gatgcgaat 
      ttcaaaaat cattcggaa gtgtgcgag ggttctttc 
      acgaaatgg ctgacaagc aacaaccat ccaaaatga 
      cagaaagaa ggttagggc aaaatggaa tcgtacagc 
      cgctactta cagaccttt atcagataa caatggctt 
      atgcatact ggaagtcgg ggaagacaa aggattacg 
      ccgtgtttg acttgttct tccgcaaaa acccttttc 
      aaggggagt tcacgattt tcgcgggtt tggaggaat 
      gtttgaaat ttttggaaa agtttcact actcgatca 
      gcgatattg agtatttga aacagactc ttccgccga 
      atattgaag aagagtttt acgagttct tgagttcgg 
      tgtccgcga aggatgtca ctttgtatg ctattgcgg 
      aaggatcag tcgcatttc ctagagtgc cacttatgc 
      gagttgaag gtcctttaa tcatagtgc aattgcttg 
      aaacgacac tgctgactt tggttaatt atgcaagtc 
      tgatggcga caaatgctg ctaggtacc ggatggcgg 
      caggaaaca tgaagttgt tcgagtttg gactgcgcc 
      gggcgcagg gccccgatg gtgggcttt cagcgtcaa 
      aatattctt acatagggg gcttcgacg gcaccagca 
      acgtattag ccgggaaac tgttcaaca tcccggtga 
      agggaaccc acgcccatg cctacatca cgtctttta 
      acggcttca ccgacttgc ggaacacca tgctggagc 
      ccaaagacg gcgccgagc cgcgggacc tcctccagc 
      tggccctct cctggagga ccaagctgg ttccgatct 
      tcaaggttg tggcttccg aagcgagcg acggcgaac 
      tggccgccc ttatttcgt tcgctattg ccttccctg 
      acggattta tggccttag ttgacacgt atgacgtta 
      agaggtatc cctctcaca tgaatacca cctccgtgt 
      ggtctgtca ctctgccca acacccaat cgcataacg 
      agtacagga gtggccttc tcaactttt gctcagtag 
      cgctagctc taaacgatt tgggatatc gggctgtgg 
      gtatccgtt tagatagtg gggacttgg cttatctat 
      cgaatgtcg ctcgccagt ttttctgcg agattagtg 
      acaagttta acattccct ggtttgagg acttgatga 
      taatggctt ccaacgaca tcaacgagg agacaatcc 
      tgagtctga acgagcaag ggcacaaaa tcaattgtt 
      tcgggatag gcacccatc tagtgactt gtcagagac 
      aaccagccc tgggctgtg tctacaagc tggtcgagc 
      tgaacggcc aacccagaa taaaactta gccaggacg 
      tcgggaaag tcacaatgc ctggacgca aagacgcct 
      atcgcctat acagcgaaa gtgggcatg ccctcgttg 
      atctgctac aaagatcaa cggaaccgc ccccagcca 
      tcaacgaaa aagtcttat gccgccatc cgttcatcg 
      agtccaaac gggcatacg ttataccat cacacgtcg 
      aaaaacttc taactcttc actggaaaa acggcgcca 
      tttgtcagc ctttaccca atcttcagc aaataaaag 
      aaagagtgc aggcatctc taaggacct taagacccg 
      acattaaga gaaacctta atcctactc ctttcaagg 
      ttgcggtca gtgacgcgc tctacactt tccttcacg 
      acttgtggc ttgaaaacg ccccaatcg gagaattat 
      cctaatcta cttaaaatg gaatagcac agttaagat 
      atcaaatgc ttcatagta caaattcaa tgttcacat 
      gttacacgt cattatcaa aagcacaag gacctgtca 
      cgtatcttg gctgtgagt tgtattatt ttattgtta 
      cggtaggag tagctttag tggaatgcc ttcgatcgg 
      cactacgaa atgtatttt attaacagt ttacatagc 
      aagaagcat cgaaatcac tgcatgttt gtactttcc 
      aaattttat aggcgactt atttgatac agcagtctg 
      ataatattt ttataattt ctcttagag acctacaca 
      ttattttgt aataaacca tacaaaacg ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY