Status
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Gene information
General information
TC031483
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001629,TC001628
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1279415..1283237
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031483
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00274

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031483 RA
      MELEDRDPN NLSRHLQVS WEDVFGEPE AIRSPECAW 
      AVSNQCFRL SKNFCYVCL SVICAPVAA FCLGITFAC 
      LSFEHIWCL TPCLRIWKI SCAATRNFV TAFAHAVII 
      PCTSACGYY WSEIKVKTQ AISPDVNEE KKDVLVVKS 
      AIRCDNKRW LNSCQLCTV CYREMSLRK LNSSRCIFN 
      AAKRLFSDK SSSKVLFNW EDPLNLESQ LTDEEKSVR 
      DSFRAYCQE KLQPRIIQA NRNETFDRE IMAEMGALG 
      VLGCTIKEY GCAGISNVG YGLLTREVE RVDSAYRSA 
      VSVQSSLVM GAIYYFGSK EQKEEYLPK LAKGEIIGC 
      FGLTEPNHG SDPSSMETR AKFNSSSKT YTLNGSKTW 
      ITNAPLADV LIIWGKNDD GKIRGFLID RKKHGKGLD 
      TPKIQGKFS LRASTTGMI LMDGVEIPE SNILPNVEG 
      LKGPFSCLN NARYGIAWG ALGAAESCL HIARNYTLE 
      RKQFGRPLA ANQLMQKKF ADMITDISL GLQGCLQVG 
      RLMDKKLHT PEMISLLKR NNAGKALVI ARVARDMLG 
      GNGIHDEYH IIRHVMNLE SVNTYEGTH DIHALILGR 
      TVTGISAF
    • >TC031483 RB
      MSLRKLNSS RCIFNAAKR LFSDKSSSK VLFNWEDPL 
      NLESQLTDE EKSVRDSFR AYCQEKLQP RIIQANRNE 
      TFDREIMAE MGALGVLGC TIKEYGCAG ISNVGYGLL 
      TREVERVDS AYRSAVSVQ SSLVMGAIY YFGSKEQKE 
      EYLPKLAKG EIIGCFGLT EPNHGSDPS SMETRAKFN 
      SSSKTYTLN GSKTWITNA PLADVLIIW GKNDDGKIR 
      GFLIDRKKH GKGLDTPKI QGKFSLRAS TTGMILMDG 
      VEIPESNIL PNVEGLKGP FSCLNNARY GIAWGALGA 
      AESCLHIAR NYTLERKQF GRPLAANQL MQKKFADMI 
      TDISLGLQG CLQVGRLMD KKLHTPEMI SLLKRNNAG 
      KALVIARVA RDMLGGNGI HDEYHIIRH VMNLESVNT 
      YEGTHDIHA LILGRTVTG ISAF
    CDS sequence
    • >TC031483 RA
      atggagttg gaagaccgc gacccgaac aacctcagc 
      cgccacttg caggtgtca tgggaagac gtttttgga 
      gaaccggaa gctatcaga agcccagaa tgcgcctgg 
      gctgtcagc aaccagtgt ttccgctta tcaaaaaac 
      ttctgctac gtctgtctt tcagttatt tgtgcccca 
      gttgccgct ttctgcctc gggatcacg ttcgcttgt 
      ttgtctttc gagcatata tggtgcctg acgccatgt 
      ttacgaatt tggaaaatt tcctgcgct gccacgagg 
      aattttgtg acggctttc gcccatgct gttatcatc 
      ccttgtacc agcgcgtgc ggctattat tggtcggaa 
      attaaagtc aaaacgcaa gcaatttcc cccgatgtt 
      aatgaggaa aagaaagac gttttagtg gtcaagtcg 
      gcgataaga tgcgataac aagagatgg cttaactct 
      tgtcagttg tgtacggtt tgttacaga gagatgtcg 
      ctacgtaaa ttaaattcg tcccggtgt atcttcaat 
      gccgccaaa agacttttc tctgacaaa tcctcgagt 
      aaagtgtta ttcaattgg gaagatcct cttaattta 
      gaatcacaa ttaaccgat gaggaaaaa agtgtgagg 
      gattctttt cgggcgtat tgccaggaa aaactgcaa 
      ccacgcata atccaagcc aatcgaaat gaaaccttt 
      gaccgcgaa attatggcg gaaatgggc gccttgggt 
      gtgctcgga tgcacgata aaagaatac ggctgtgcg 
      ggaatttca aatgtaggc tacggcctc ctcactcga 
      gaggtcgaa agggttgac agtgcctac agatcagca 
      gtcagcgtc cagtcatca ctcgtaatg ggcgcaatc 
      tattacttc gggtcaaaa gaacagaaa gaagagtat 
      ttgcccaaa ctcgcgaaa ggagaaatc atcggttgt 
      ttcgggctg acggaaccc aaccatggt agcgaccca 
      agttcgatg gaaacgcgc gctaagttc aactcatcc 
      tcgaagacg tacaccctg aacggtagc aaaacctgg 
      attacgaac gcgccactt gccgacgtt ttaattatt 
      tggggcaaa aacgacgac gggaaaatc cgcggtttt 
      ctaatcgac aggaaaaag cacgggaag gggttagac 
      acccctaaa atccagggg aagttctcg ctccgcgcc 
      tccaccacg ggcatgatc ctaatggac ggggtggaa 
      atccccgaa agcaacatt ttgccgaac gttgagggg 
      ctcaaaggg ccgttctcg tgcctgaac aacgcccgc 
      tacggcatt gcctggggg gccctgggg gccgccgag 
      tcgtgtctc cacatcgcc cggaactac accctcgaa 
      aggaagcag ttcgggcgc cccctggcc gccaaccaa 
      ctcatgcaa aaaaaattc gcggacatg attaccgac 
      atttcgctc gggctgcaa gggtgcctc caagtcggg 
      cgcttgatg gataaaaaa ttacacacg cctgagatg 
      atttcgctc ctgaagaga aacaacgcg gggaaagct 
      ttggtaatc gcgagggtc gcaagggac atgttgggc 
      ggaaacgga attcacgac gagtaccac atcatccga 
      catgtaatg aatttggag tcggtcaac acgtacgaa 
      ggtacccac gacatccat gccttgatt ttgggacgc 
      accgtcacg ggaatatcc gctttttaa
    • >TC031483 RB
      atgtcgcta cgtaaatta aattcgtcc cggtgtatc 
      ttcaatgcc gccaaaaga cttttctct gacaaatcc 
      tcgagtaaa gtgttattc aattgggaa gatcctctt 
      aatttagaa tcacaatta accgatgag gaaaaaagt 
      gtgagggat tcttttcgg gcgtattgc caggaaaaa 
      ctgcaacca cgcataatc caagccaat cgaaatgaa 
      acctttgac cgcgaaatt atggcggaa atgggcgcc 
      ttgggtgtg ctcggatgc acgataaaa gaatacggc 
      tgtgcggga atttcaaat gtaggctac ggcctcctc 
      actcgagag gtcgaaagg gttgacagt gcctacaga 
      tcagcagtc agcgtccag tcatcactc gtaatgggc 
      gcaatctat tacttcggg tcaaaagaa cagaaagaa 
      gagtatttg cccaaactc gcgaaagga gaaatcatc 
      ggttgtttc gggctgacg gaacccaac catggtagc 
      gacccaagt tcgatggaa acgcgcgct aagttcaac 
      tcatcctcg aagacgtac accctgaac ggtagcaaa 
      acctggatt acgaacgcg ccacttgcc gacgtttta 
      attatttgg ggcaaaaac gacgacggg aaaatccgc 
      ggttttcta atcgacagg aaaaagcac gggaagggg 
      ttagacacc cctaaaatc caggggaag ttctcgctc 
      cgcgcctcc accacgggc atgatccta atggacggg 
      gtggaaatc cccgaaagc aacattttg ccgaacgtt 
      gaggggctc aaagggccg ttctcgtgc ctgaacaac 
      gcccgctac ggcattgcc tggggggcc ctgggggcc 
      gccgagtcg tgtctccac atcgcccgg aactacacc 
      ctcgaaagg aagcagttc gggcgcccc ctggccgcc 
      aaccaactc atgcaaaaa aaattcgcg gacatgatt 
      accgacatt tcgctcggg ctgcaaggg tgcctccaa 
      gtcgggcgc ttgatggat aaaaaatta cacacgcct 
      gagatgatt tcgctcctg aagagaaac aacgcgggg 
      aaagctttg gtaatcgcg agggtcgca agggacatg 
      ttgggcgga aacggaatt cacgacgag taccacatc 
      atccgacat gtaatgaat ttggagtcg gtcaacacg 
      tacgaaggt acccacgac atccatgcc ttgattttg 
      ggacgcacc gtcacggga atatccgct ttttaa
    mRNA sequence
    • >TC031483 RA
      cgatttaaa accctggct tccattttc cctcgaact 
      tcaaattct tccagctct ccaatggcg aaagaaagt 
      gacacaatg gagttggaa gaccgcgac ccgaacaac 
      ctcagccgc cacttgcag gtgtcatgg gaagacgtt 
      tttggagaa ccggaagct atcagaagc ccagaatgc 
      gcctgggct gtcagcaac cagtgtttc cgcttatca 
      aaaaacttc tgctacgtc tgtctttca gttatttgt 
      gccccagtt gccgctttc tgcctcggg atcacgttc 
      gcttgtttg tctttcgag catatatgg tgcctgacg 
      ccatgttta cgaatttgg aaaatttcc tgcgctgcc 
      acgaggaat tttgtgacg gctttcgcc catgctgtt 
      atcatccct tgtaccagc gcgtgcggc tattattgg 
      tcggaaatt aaagtcaaa acgcaagca atttccccc 
      gatgttaat gaggaaaag aaagacgtt ttagtggtc 
      aagtcggcg ataagatgc gataacaag agatggctt 
      aactcttgt cagttgtgt acggtttgt tacagagag 
      atgtcgcta cgtaaatta aattcgtcc cggtgtatc 
      ttcaatgcc gccaaaaga cttttctct gacaaatcc 
      tcgagtaaa gtgttattc aattgggaa gatcctctt 
      aatttagaa tcacaatta accgatgag gaaaaaagt 
      gtgagggat tcttttcgg gcgtattgc caggaaaaa 
      ctgcaacca cgcataatc caagccaat cgaaatgaa 
      acctttgac cgcgaaatt atggcggaa atgggcgcc 
      ttgggtgtg ctcggatgc acgataaaa gaatacggc 
      tgtgcggga atttcaaat gtaggctac ggcctcctc 
      actcgagag gtcgaaagg gttgacagt gcctacaga 
      tcagcagtc agcgtccag tcatcactc gtaatgggc 
      gcaatctat tacttcggg tcaaaagaa cagaaagaa 
      gagtatttg cccaaactc gcgaaagga gaaatcatc 
      ggttgtttc gggctgacg gaacccaac catggtagc 
      gacccaagt tcgatggaa acgcgcgct aagttcaac 
      tcatcctcg aagacgtac accctgaac ggtagcaaa 
      acctggatt acgaacgcg ccacttgcc gacgtttta 
      attatttgg ggcaaaaac gacgacggg aaaatccgc 
      ggttttcta atcgacagg aaaaagcac gggaagggg 
      ttagacacc cctaaaatc caggggaag ttctcgctc 
      cgcgcctcc accacgggc atgatccta atggacggg 
      gtggaaatc cccgaaagc aacattttg ccgaacgtt 
      gaggggctc aaagggccg ttctcgtgc ctgaacaac 
      gcccgctac ggcattgcc tggggggcc ctgggggcc 
      gccgagtcg tgtctccac atcgcccgg aactacacc 
      ctcgaaagg aagcagttc gggcgcccc ctggccgcc 
      aaccaactc atgcaaaaa aaattcgcg gacatgatt 
      accgacatt tcgctcggg ctgcaaggg tgcctccaa 
      gtcgggcgc ttgatggat aaaaaatta cacacgcct 
      gagatgatt tcgctcctg aagagaaac aacgcgggg 
      aaagctttg gtaatcgcg agggtcgca agggacatg 
      ttgggcgga aacggaatt cacgacgag taccacatc 
      atccgacat gtaatgaat ttggagtcg gtcaacacg 
      tacgaaggt acccacgac atccatgcc ttgattttg 
      ggacgcacc gtcacggga atatccgct ttttaatca 
      ttttgtacc aatgaatga aataaatct ttgctcttg 
      at
    • >TC031483 RB
      ctcgacggg tataaaaat agtaacttc gtcaattga 
      atgttggcc gactgtaaa accaactaa ttccattgc 
      gagaatttt gagagatgt cgctacgta aattaaatt 
      cgtcccggt gtatcttca atgccgcca aaagacttt 
      tctctgaca aatcctcga gtaaagtgt tattcaatt 
      gggaagatc ctcttaatt tagaatcac aattaaccg 
      atgaggaaa aaagtgtga gggattctt ttcgggcgt 
      attgccagg aaaaactgc aaccacgca taatccaag 
      ccaatcgaa atgaaacct ttgaccgcg aaattatgg 
      cggaaatgg gcgccttgg gtgtgctcg gatgcacga 
      taaaagaat acggctgtg cgggaattt caaatgtag 
      gctacggcc tcctcactc gagaggtcg aaagggttg 
      acagtgcct acagatcag cagtcagcg tccagtcat 
      cactcgtaa tgggcgcaa tctattact tcgggtcaa 
      aagaacaga aagaagagt atttgccca aactcgcga 
      aaggagaaa tcatcggtt gtttcgggc tgacggaac 
      ccaaccatg gtagcgacc caagttcga tggaaacgc 
      gcgctaagt tcaactcat cctcgaaga cgtacaccc 
      tgaacggta gcaaaacct ggattacga acgcgccac 
      ttgccgacg ttttaatta tttggggca aaaacgacg 
      acgggaaaa tccgcggtt ttctaatcg acaggaaaa 
      agcacggga aggggttag acaccccta aaatccagg 
      ggaagttct cgctccgcg cctccacca cgggcatga 
      tcctaatgg acggggtgg aaatccccg aaagcaaca 
      ttttgccga acgttgagg ggctcaaag ggccgttct 
      cgtgcctga acaacgccc gctacggca ttgcctggg 
      gggccctgg gggccgccg agtcgtgtc tccacatcg 
      cccggaact acaccctcg aaaggaagc agttcgggc 
      gccccctgg ccgccaacc aactcatgc aaaaaaaat 
      tcgcggaca tgattaccg acatttcgc tcgggctgc 
      aagggtgcc tccaagtcg ggcgcttga tggataaaa 
      aattacaca cgcctgaga tgatttcgc tcctgaaga 
      gaaacaacg cggggaaag ctttggtaa tcgcgaggg 
      tcgcaaggg acatgttgg gcggaaacg gaattcacg 
      acgagtacc acatcatcc gacatgtaa tgaatttgg 
      agtcggtca acacgtacg aaggtaccc acgacatcc 
      atgccttga ttttgggac gcaccgtca cgggaatat 
      ccgcttttt aatcatttt gtaccaatg aatgaaata 
      aatctttgc tcttgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY