Status
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Gene information
General information
TC001623 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1344211..1350089
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001623
    iBeetle screen iB_00273

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001623 RA
      MNLFGCLFP CALQHSWWS VGNEQCMTE EFVHRMGVN 
      ASTAPGVEA GPMPNSMGV ADPCTPPFR DTIGVIVRE 
      RKKKVTGFA TLKKKFIRR RRSSKACDH GRMLRELVS 
      DWSPLEVAA LLEEYEALA ALKDLCVQA ELARPPAAT 
      FKQDLSALY DFKYCTDAD LVYRGACFP IHRALLSAR 
      CPYFRDLLA GCPGYGARI CLELRTATV DVQLFSALL 
      RYLYTGDIC PHDTNIDIN LLRRLREEF GTPNALEAD 
      LRYLLEIGD YADAALVFT SDGSDCQRP DSGSSEYGF 
      RPKLELPCH KAILSARSP FFRNLLQRR SRSGEEHTE 
      RALHIPTRI VLDESVIPK RYARVLLHA IYLDHVDLS 
      LISRGGCGG GLGEAQALT HTGRARPSP LEEAMELYQ 
      IGRFLELDI LSQGCEDLI LEWLSLDTL PTVLQWAKQ 
      PHGSAWVHR QALHYLREE FQPVAQSPV LHQLDKAHL 
      IEVLKSPFL QASELEILH AVLKWGENE LVRRMEDRE 
      PNIVSHTAH SVARKGVKK RDLSDVELR EILSDLLPL 
      VRMDHVLPP NSDILNQAI RRGLVSTPP SHMIGGDRE 
      NLRVNAWIR GDKNNGLFV QPRLFMPYY DEVKVLVED 
      QLVQEVEIM RMRRRYMPD IPDTLYMVE DKPRVHGAA 
      GVDVLASVL PVPDPAVMN AMLKREQKL RQSPSCQRA 
      LNMPLSSRH EINRQIRLR VVREFNLPD PVADMLENC 
      YCLPESDDN AESHTISPN KGLRGCYSR NMTFPRPGA 
      YSQRHELPA VIPEGEFRF ATEPESSSC SDGHLSDIM 
      PDVAMATAT LGQMQLQEQ QEMELDLGD GATHLHAQM 
      AGSLHHPMS YRRYQPSTY PHLRSDSQS YHTPMPRFL
    CDS sequence
    • >TC001623 RA
      atgaactta tttggctgc ctctttccg tgtgcactg 
      cagcacagt tggtggagt gttggcaac gagcagtgc 
      atgactgag gagttcgtc cacaggatg ggcgtgaac 
      gcttccacg gcacccggt gtcgaggct ggtcctatg 
      cccaacagc atgggagtc gcggatcct tgcacgcct 
      ccgttcagg gacacgatt ggggtgatc gtacgggag 
      cgtaaaaag aaagtgact ggttttgcc accttgaag 
      aagaagttc atacgtcgg agaagatcg tcgaaggcg 
      tgcgaccac ggaaggatg ctgcgcgag ctggtgtct 
      gactggagt cctctggag gtggcggcc ttgctggag 
      gagtacgag gcgttggcc gctttgaaa gatctctgt 
      gttcaagcc gagctggcg cggccgccg gccgccacc 
      ttcaagcag gacctctcc gccttgtac gacttcaag 
      tactgcacc gatgccgat ttggtgtac agaggggct 
      tgctttccc atccaccgc gccctactc tccgccagg 
      tgcccttat tttagagac ttactcgcc gggtgtccc 
      ggctacggc gctcgaata tgtctcgaa ttgagaacg 
      gccacggtc gatgtccag ctgttctcg gcgcttttg 
      cgatatctg tacaccggg gacatctgt ccgcacgat 
      accaacatc gatatcaac ctcctgagg cggctgcga 
      gaggagttc ggaacaccc aatgccttg gaagccgat 
      ctgcgttac ttacttgaa attggcgac tacgccgat 
      gctgctcta gtgttcact tccgacggt tccgactgc 
      cagaggccc gattcgggc agttcggaa tacggtttt 
      aggccaaaa ttagaatta ccatgccac aaagccata 
      ctctccgct cgatcgccc ttcttccgg aacctgcta 
      caaaggcga tcgaggagc ggcgaggag cacacagaa 
      cgagcctta cacatacct accagaatc gtactcgac 
      gaatcagtc atacctaaa cggtacgca cgcgttcta 
      ctacacgct atttattta gatcacgtt gatctctcg 
      ttaatttca cgcggtggc tgtgggggc ggtctaggc 
      gaagcccaa gcacttact cacaccgga cgggcgcgg 
      ccgtcaccc ctggaggaa gccatggag ttatatcaa 
      atcggacgg ttcctcgaa ctagatatt ctctcacaa 
      ggctgtgaa gatttaatt ttagaatgg ttgtcgctt 
      gacacactc cccacagta cttcaatgg gccaaacaa 
      ccgcacgga tcggcctgg gtccaccga caggcgctg 
      cattacttg cgcgaggag ttccaaccg gtggcccag 
      tccccggtg ttgcaccaa ctggacaag gctcacctg 
      atcgaggtg ttgaaaagt ccgtttttg caagcctcc 
      gagctggaa atcctccac gctgtactg aaatggggc 
      gagaacgag ttggttcgt aggatggag gatcgggaa 
      ccgaacatt gtgagtcac accgcgcat tcggtggcc 
      cgcaaaggt gtgaaaaag cgcgatttg agtgatgtc 
      gagcttcgc gagatttta tccgatttg cttccattg 
      gtccgcatg gaccacgta ctgcccccc aatagtgat 
      attctaaac caggcgatt cgacgtggc ttggttagc 
      actcccccc agccatatg ataggcggc gaccgggag 
      aatctgagg gtgaacgcg tggattagg ggtgataag 
      aataatggt ttattcgta caaccgcgc ctgtttatg 
      ccatactat gacgaggtt aaggttctt gtggaagat 
      cagttggtc caagaagtt gaaataatg cgaatgcgc 
      cgccggtac atgcccgac attcccgat acgctgtac 
      atggtcgag gacaagcca cgggtgcat ggggccgcc 
      ggggttgat gttttggct tcggttctt ccagtgcca 
      gacccggcg gttatgaac gcaatgttg aaacgggaa 
      caaaaactc cgccaatcg ccgagctgt caacgtgcc 
      cttaacatg cctttatcc tcacgtcat gaaatcaac 
      cggcagatc cggctccgg gtcgtccgc gagttcaat 
      ctgcctgac ccagtggcc gatatgttg gagaactgc 
      tattgtctc cctgagagc gatgataat gctgagagc 
      cacacaata tcgccgaat aaggggctc agggggtgc 
      tatagtcgc aatatgacg tttccgagg cctggggcg 
      tactcgcag aggcacgag ctgccggcg gtgatcccc 
      gagggggag ttccggttt gcgacggag cccgagagc 
      agttcgtgc agcgatggg catttgtcg gatataatg 
      cccgacgtg gcgatggcg acggcgacc ttgggccag 
      atgcagctg caggagcag caggagatg gagctggac 
      ttgggggat ggggccacc catctgcac gcccagatg 
      gccgggtcg ctgcaccac ccgatgtcc tacaggcgg 
      taccaaccc tcgacgtac cctcacctg aggtctgat 
      tcgcaatct taccacact cctatgccg cgattttta 
      tag
    mRNA sequence
    • >TC001623 RA
      gatcaattt tttattttt ttttatgat taataaaaa 
      ataatggac gtgggttga ataacaata atcaaattc 
      gctgaaaat attgacatg aattattgt ggcgagtgg 
      cgacccagt ttggagttt gtgtagccg gtgtcaatg 
      taggtgaaa tgaagttgc tccaaacaa gagaaatcg 
      tcgtttgtg ttccctatg tgctgagag actcgcaaa 
      tcggtggtt aatatatga aaaaagggc agtgttcca 
      cgcagaaaa cggcagcaa gatgtagga agtgttcgt 
      ttcggccgc tgttaatat ttccagtgt ttatgtggc 
      acagataat tgttttcga cttggacga tataaacat 
      ggctactat tcagactcc tcgaactct ttcggctta 
      aaatcgcct taaatggac gttaatggt acaaataca 
      gaagtacgc cgagaaagc agtatgatt atgacatgc 
      ccaggctgt gtctgcatg tcaggctgt agtctgggc 
      aaactatga acttatttg gctgcctct ttccgtgtg 
      cactgcagc acagttggt ggagtgttg gcaacgagc 
      agtgcatga ctgaggagt tcgtccaca ggatgggcg 
      tgaacgctt ccacggcac ccggtgtcg aggctggtc 
      ctatgccca acagcatgg gagtcgcgg atccttgca 
      cgcctccgt tcagggaca cgattgggg tgatcgtac 
      gggagcgta aaaagaaag tgactggtt ttgccacct 
      tgaagaaga agttcatac gtcggagaa gatcgtcga 
      aggcgtgcg accacggaa ggatgctgc gcgagctgg 
      tgtctgact ggagtcctc tggaggtgg cggccttgc 
      tggaggagt acgaggcgt tggccgctt tgaaagatc 
      tctgtgttc aagccgagc tggcgcggc cgccggccg 
      ccaccttca agcaggacc tctccgcct tgtacgact 
      tcaagtact gcaccgatg ccgatttgg tgtacagag 
      gggcttgct ttcccatcc accgcgccc tactctccg 
      ccaggtgcc cttatttta gagacttac tcgccgggt 
      gtcccggct acggcgctc gaatatgtc tcgaattga 
      gaacggcca cggtcgatg tccagctgt tctcggcgc 
      ttttgcgat atctgtaca ccggggaca tctgtccgc 
      acgatacca acatcgata tcaacctcc tgaggcggc 
      tgcgagagg agttcggaa cacccaatg ccttggaag 
      ccgatctgc gttacttac ttgaaattg gcgactacg 
      ccgatgctg ctctagtgt tcacttccg acggttccg 
      actgccaga ggcccgatt cgggcagtt cggaatacg 
      gttttaggc caaaattag aattaccat gccacaaag 
      ccatactct ccgctcgat cgcccttct tccggaacc 
      tgctacaaa ggcgatcga ggagcggcg aggagcaca 
      cagaacgag ccttacaca tacctacca gaatcgtac 
      tcgacgaat cagtcatac ctaaacggt acgcacgcg 
      ttctactac acgctattt atttagatc acgttgatc 
      tctcgttaa tttcacgcg gtggctgtg ggggcggtc 
      taggcgaag cccaagcac ttactcaca ccggacggg 
      cgcggccgt cacccctgg aggaagcca tggagttat 
      atcaaatcg gacggttcc tcgaactag atattctct 
      cacaaggct gtgaagatt taattttag aatggttgt 
      cgcttgaca cactcccca cagtacttc aatgggcca 
      aacaaccgc acggatcgg cctgggtcc accgacagg 
      cgctgcatt acttgcgcg aggagttcc aaccggtgg 
      cccagtccc cggtgttgc accaactgg acaaggctc 
      acctgatcg aggtgttga aaagtccgt ttttgcaag 
      cctccgagc tggaaatcc tccacgctg tactgaaat 
      ggggcgaga acgagttgg ttcgtagga tggaggatc 
      gggaaccga acattgtga gtcacaccg cgcattcgg 
      tggcccgca aaggtgtga aaaagcgcg atttgagtg 
      atgtcgagc ttcgcgaga ttttatccg atttgcttc 
      cattggtcc gcatggacc acgtactgc cccccaata 
      gtgatattc taaaccagg cgattcgac gtggcttgg 
      ttagcactc cccccagcc atatgatag gcggcgacc 
      gggagaatc tgagggtga acgcgtgga ttaggggtg 
      ataagaata atggtttat tcgtacaac cgcgcctgt 
      ttatgccat actatgacg aggttaagg ttcttgtgg 
      aagatcagt tggtccaag aagttgaaa taatgcgaa 
      tgcgccgcc ggtacatgc ccgacattc ccgatacgc 
      tgtacatgg tcgaggaca agccacggg tgcatgggg 
      ccgccgggg ttgatgttt tggcttcgg ttcttccag 
      tgccagacc cggcggtta tgaacgcaa tgttgaaac 
      gggaacaaa aactccgcc aatcgccga gctgtcaac 
      gtgccctta acatgcctt tatcctcac gtcatgaaa 
      tcaaccggc agatccggc tccgggtcg tccgcgagt 
      tcaatctgc ctgacccag tggccgata tgttggaga 
      actgctatt gtctccctg agagcgatg ataatgctg 
      agagccaca caatatcgc cgaataagg ggctcaggg 
      ggtgctata gtcgcaata tgacgtttc cgaggcctg 
      gggcgtact cgcagaggc acgagctgc cggcggtga 
      tccccgagg gggagttcc ggtttgcga cggagcccg 
      agagcagtt cgtgcagcg atgggcatt tgtcggata 
      taatgcccg acgtggcga tggcgacgg cgaccttgg 
      gccagatgc agctgcagg agcagcagg agatggagc 
      tggacttgg gggatgggg ccacccatc tgcacgccc 
      agatggccg ggtcgctgc accacccga tgtcctaca 
      ggcggtacc aaccctcga cgtaccctc acctgaggt 
      ctgattcgc aatcttacc acactccta tgccgcgat 
      ttttatagt tttaattct ccttttcgc tttcgtgtc 
      ttgtacata agagaagca ttcttttcg ggaataata 
      tacatctgt gattttctg tgacgagac atctctagt 
      tttaatatt tattagatc gtttctttg ttttcgaga 
      ctgatccga gattctttc gattgcaga tgatgcccg 
      agtaatact gagagttgg ttgtttaac tgatcaaca 
      tattccgtt aattctgta ttcatgtac ctgtgcgtg 
      tcttgtctt agatgttgc tgtgttgct gttacatgt 
      aaatatatt tagagtcca agtgacaac tcttaaacg 
      gtgcgattt tatccatta ctaagtgca atgtgtact 
      taaacactc tcataacga ctatcatta actgtttca 
      acaacgaac atttcattc tacagtttg ctttttatt 
      gctactggt ttcttactt tttgcatat cattcgggg 
      agagagctg gaaacggac cgaatcgtg agggtggca 
      aattgaatc tcaacttaa cttaagctc ttactactt 
      aagacttgg cgcatttac gttaattag tttacagct 
      ccctctttt aatttagtt aaattgtct gtgattaat 
      gtttctttt gaagttgct acatataac atttcttga 
      tcggttgat gttgcagat caccatttt gtgatatgt 
      attgtaatc tacgttgat gtaatcaca ttattgtta 
      tacatacat gttagctta gaactgtat ggtttatag 
      gcttctatg ggtaattat tatttaata gaatcctaa 
      atgtactca atcggataa ttcttgcga ttcagggta 
      tccacataa acatgaaaa atgtttatt tgtgatatg 
      tactttgta ttcattgat tcgattatg ttgctacgt 
      tttaaatac ttttgcaaa ctgcagtat gttacattt 
      caatataaa tattaacac aaatacgaa agacattat 
      agcttatta cataattta tttcaaaat gtaaatata 
      ttaaataca aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY