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Gene information
General information
TC001622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1094934..1099445
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001622
    iBeetle screen iB_00272
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles anterior shortened - Number of animals showing the phenotype: 3

    oblique dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 2

    Adult morphology
    wings orientation irregular - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001622 RA
      MPVRKAIHA GSWYTDSGP ELSRQLEYW LHQADLIHG 
      PARAIIAPH AGYQYCGAC GGHAYRQVS PVVVRRIFI 
      LGPSHHVRL SGCALSTAR KYKTPLYDL HVDSQVNGE 
      LEMTGQFEW MDLDTDENE HSIEMHLPY IAKVMENYK 
      HQFTIVPIL VGSLSPERE ACYGRILSS YLGDPQNLF 
      IISSDFCHW GHRFRYTYY DRSYGSIYQ SIEELDRLG 
      MNIIENLNP TDFTNYLKK FGNTICGRH PIGILLQAV 
      QELLRNDSR RNANLKFLK YAQSSQCRN MNDSSVSYA 
      SAALVFE
    CDS sequence
    • >TC001622 RA
      atgcctgtt agaaaagcc atacatgct ggtagttgg 
      tacacagac tcaggtccc gaattatcg cggcagctc 
      gaatattgg ctgcaccaa gccgactta atccacggt 
      cctgctcgg gctataatc gctccacat gcgggctat 
      cagtactgc ggcgcttgc ggggggcac gcgtacaga 
      caagtcagt cctgtcgtg gtaaggcgt atatttatt 
      ttaggacct tctcaccat gtccgttta tcaggatgt 
      gccttgtcc acagcgcga aaatacaag acccccctt 
      tacgactta catgtagat tctcaagta aacggcgaa 
      ttagaaatg acgggtcag ttcgaatgg atggactta 
      gatactgac gaaaatgag cacagtatt gaaatgcat 
      ttaccctac atagcgaaa gttatggaa aattacaaa 
      caccaattc accatagta cctattctt gtgggttcg 
      ctcagtccc gagcgggag gcttgctac ggtcggata 
      ctatcgagc tacctagga gaccctcaa aaccttttc 
      ataatatcg tcagatttc tgccactgg ggccaccgg 
      tttcgctac acgtattac gaccgttct tacggcagc 
      atctaccaa tcgatagaa gaactcgat cgtttgggg 
      atgaacata atagaaaat ctaaatccg accgacttc 
      acgaattat ttaaagaaa ttcggaaac acgatttgt 
      ggtagacat ccgatcggg atactactg caggccgtg 
      caggagctc ctgcggaac gacagtcgg agaaacgcg 
      aatttgaaa ttcctgaaa tatgctcag tcaagtcag 
      tgtcgtaat atgaatgac tcgtcagtg agttacgca 
      tcggctgcc cttgtgttc gagtag
    mRNA sequence
    • >TC001622 RA
      ggggtttaa cctaactta taacagcta atacgtaag 
      atggtaaat cagttatta cagttgggt aaatttact 
      attttacgt tgtggaata acatcacga ctgagcttc 
      gtttaataa ttcccgttt aaccatttt ttgtccact 
      gttttttaa ttcttcagt tgttccttt tggtgtcag 
      ttcctcatt taccaattt ttaacccaa ctgcatgtt 
      tcattttat ttcctctaa aggtgatgg gtaactttt 
      ccccgtctt tccattata attttcatt aattcattt 
      catttttcc tactaatgg atcaagaaa cttgttatt 
      ttctccagt aaaaggtaa ggaaatgat attattaac 
      ggctgtccg ctccgcgga tgattttat attatgata 
      atatttatt gtaatataa acgtttgaa tcttttaag 
      agaccagtc tcgcaaatt ttgtagaaa agaaatatt 
      taaatttca gttattcaa ttatctgat gtggtcgca 
      aactgaaat ttaaatact gtgaacatt ctacgtctt 
      cttttctaa gccagtatt ttcattttt atagatctc 
      ggtgcctat taatcgttt gtttgacat tttaaagaa 
      tggttaaat tttttgttt cagtctcac gaagtactt 
      caacccacg gaaaacgaa tcgtattac tatattagt 
      aattcgggc cagtacttg ctcactgtt gcggaattc 
      aaagatatt gttagaact ctcgaaaat tctgagaat 
      aatctactc attttcttt ttagaaatg ttttctaaa 
      gtgaaagta tcttaaact catgcctgg atttttaca 
      ggtgtctta gtagtaata acaactttt ttaaatatg 
      ttgatcaac cgtgaaata cacttgtag tattattta 
      ctacgaagt tttacgacg tctgatacc gtgttaata 
      gacttaaaa tttaacttt ttaaccgaa tctttgatt 
      tcacctcag tatcacttt tcctgttga tgctaagcg 
      tgaaatatc ggtgttttt tcactttgt gctaggttt 
      tgattcttg tcagtacaa gggtttagt ttgtgttta 
      aaataattg catttttcg gtccgccat gttgtcggc 
      gttaaagtg ttgctcagt tcgcagctg cgcagcttt 
      tcgttacct aataggttg tttcactca gcttaagtg 
      tatttgtgt tgtttcatt ttgaatttg aaatatagt 
      ttggtttgt aaaactttg ttttctatt tttaacttg 
      aaaatcgca cgcttttca ttcggtaaa attagtgag 
      aattacttg acttcttcg ccgaatttc tttgcgcca 
      ttccgtaac aagttttat tctgtgaaa gaacatatc 
      gatctctaa acaattttc ggaattact tctcggata 
      aagtttgga aaccgccgc agtcgtgcc caagccaat 
      tttaggtcc ctaagaaac acgaagcac cttaaataa 
      atgtcgtcg gaagactcg gagaggaag atttcgtta 
      aaaaatcga atgcgtttc gcagtttta ttgaggtta 
      ctttaggaa actttattt aaaatcacc aggtttgta 
      tttttttgt taaatattt aaataagta gctattttg 
      gttgaaaca actgacgca ttgaaatag tctatttgc 
      tacaaggtg gttataaat gatttccat caagtgggc 
      cggtggcga cttgatgga aaatcattt ataatcact 
      ttgtagttg ccgacacgg tgaaataca tggggcggg 
      gcttgcgaa agcgacgca cgccattgg ctcatatat 
      tttcactgt gattctaac gcccttctt ctcgaataa 
      cttaactta tgcgaaaat agaatataa tcggcctac 
      tcatacttg aaatgtcga actatatat aattctctg 
      atcaataac tagtcactt tttattcta cattaaatg 
      ggaggctat gcaaaactt gatcaaaat tagctgtca 
      tagccaact gtattgcca tattcaacc aagcgaatt 
      gatttgagt ttttggcaa caatatatt gtattgatt 
      ttaattgtg attttgatg ttcacaggt acataaaaa 
      atcgcaccc tccgcgata tcgaagcaa aggctgagg 
      attaggtac cacccgagc ccgaaggac tacgactgt 
      gatattctg tacctgatt ttattaaaa aatatattt 
      tttactcga gtttttatc ccccctcac ctttgttac 
      tgaatagtg tagtcacaa aagacatgg tgcacaccg 
      gaagtggtt ccaaaaatt ttgcaaaaa atttgaagc 
      gaaaaaatc gtactggcg gcgcatgcg tgccatcaa 
      ttagccgac gctcatgcg tgttgtcga atttccacg 
      aaaagtgtt gtctatttt agcaagtaa tctagtatt 
      aaaaattca tttgtttgt ttgatttcg agtttgcgg 
      atccaggcg accaaaaaa tgcctgtta gaaaagcca 
      tacatgctg gtagttggt acacagact caggtcccg 
      aattatcgc ggcagctcg aatattggc tgcaccaag 
      ccgacttaa tccacggtc ctgctcggg ctataatcg 
      ctccacatg cgggctatc agtactgcg gcgcttgcg 
      gggggcacg cgtacagac aagtcagtc ctgtcgtgg 
      taaggcgta tatttattt taggacctt ctcaccatg 
      tccgtttat caggatgtg ccttgtcca cagcgcgaa 
      aatacaaga ccccccttt acgacttac atgtagatt 
      ctcaagtaa acggcgaat tagaaatga cgggtcagt 
      tcgaatgga tggacttag atactgacg aaaatgagc 
      acagtattg aaatgcatt taccctaca tagcgaaag 
      ttatggaaa attacaaac accaattca ccatagtac 
      ctattcttg tgggttcgc tcagtcccg agcgggagg 
      cttgctacg gtcggatac tatcgagct acctaggag 
      accctcaaa accttttca taatatcgt cagatttct 
      gccactggg gccaccggt ttcgctaca cgtattacg 
      accgttctt acggcagca tctaccaat cgatagaag 
      aactcgatc gtttgggga tgaacataa tagaaaatc 
      taaatccga ccgacttca cgaattatt taaagaaat 
      tcggaaaca cgatttgtg gtagacatc cgatcggga 
      tactactgc aggccgtgc aggagctcc tgcggaacg 
      acagtcgga gaaacgcga atttgaaat tcctgaaat 
      atgctcagt caagtcagt gtcgtaata tgaatgact 
      cgtcagtga gttacgcat cggctgccc ttgtgttcg 
      agtagtcag tggccttac agctatcac attgactgt 
      tattaaaat ggaacacct tgattaatg attgtgcga 
      aatttttta actacccta gggataatt acgttgatt 
      ttaagctta tcgtaacga ttaattttt gtaaagcta 
      aattctaaa acgatagtt taggtttgt taagtttat 
      tttttacta acggttcca ccaagtgac atcactgtg 
      agttggcgc gtttcggga cggacaaga tcaattcaa 
      ttttatcgt gttatattt gttaaatgt ggtggtttg 
      caatattag tataaggaa ggttgtgaa attttgcat 
      cgtaggtcc cccgaatgt tttgtttta tgcttgcta 
      tgcagtaca actctaatg atctggagg gtgttgatc 
      gcttggtag atgtattta agagtacca aaatgttag 
      aataatctt tatatagtg gatctggat attgggagt 
      tatgctgta ttttcagtc ctgtgagca tagtgatag 
      gctaggagt ttggtttat tatgttctc gtaattagc 
      ttgccggcg tttatagga ctaaaatca gcgcgaatt 
      taattaatt tctaccttt atataaatt ttaatagag 
      tatgtgcta atgttaggg gtatgaata tacaataaa 
      ttgtcctac tgtaggcct acggttcgt aagcctaaa 
      gactgtaaa tttacaata tgaagtaca tatgccttt 
      aatcaatca atctattgg atgtataaa ctgtgaatg 
      caattttat atcattctt gtaacacga aataaatgt 
      gaaaaatat tt