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Gene information
General information
TC001606 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1264049..1265980
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001606
    iBeetle screen iB_00270

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001606 RA
      MNSYADNIT ASLENSRNS LRRIARNDD 
      TEFSHQSIL NLMEKFVKT VNAMDETIL 
      VPCRLMDLK VGDENDPTC PKKHNQKSK 
      HGVQELLSS ADLFQIYNM LNSVKADLL 
      WGQGQAAEE APAPAPAAT VVKGHVRRP 
      STVSVASTN SSASTISDS ESDAGNEND 
      SGIEESQEC FGDRTEQVA QNFRRHLVG 
      LTRSLKQMT EAAQYLTSR YQHDIGGPV
    CDS sequence
    • >TC001606 RA
      atgaattcc tatgcagat aatatcaca gcaagcttg 
      gaaaacagc agaaacagc cttcggcgc atcgcccgc 
      aacgacgac acggaattc tcgcaccag agcattttg 
      aacctgatg gagaagttc gtgaagacg gtgaacgcg 
      atggacgag acaatccta gtgccttgc cgcctgatg 
      gacctgaag gtgggggac gagaacgac ccgacgtgt 
      cccaaaaag cataaccaa aagtcaaaa cacggtgtc 
      caagagctg ctgagttcc gccgatctg ttccaaatc 
      tacaacatg ctgaacagc gtgaaggcc gatttgctg 
      tggggacaa ggacaggcg gccgaggag gctccggct 
      ccggccccg gcggcgaca gtggtcaag ggacacgtg 
      cgaagaccg tcgactgtc agcgtagcc tcgacgaat 
      tcgtcggcg tcgacgatc agcgactcc gagtcggac 
      gccgggaac gagaacgac tccggaatc gaggaatcg 
      caggagtgt ttcggggat cggacggaa caagtcgcg 
      caaaacttc aggaggcat ctggtggga ctcacccgg 
      tcgctgaaa cagatgacc gaagctgcc cagtacctc 
      acttcgcgt tatcagcac gatatcgga gggccagtg 
      tga
    mRNA sequence
    • >TC001606 RA
      aggcgattg cgggcgatt tgtccccaa ttcccaaac 
      agccgaaaa aacaaaccc ggggctgtt cccgccccg 
      cgggtccct cccgtgggt gcaaacgca ccaaaggtg 
      aactcctcg cagctatcg tttgtgttt gaatagttt 
      tttgaataa aatcagaga tcacgacct ctgtacgtc 
      gatactacc gtcacgcct tctccagag ccccactcc 
      tgtcatcag tcatcctca tataaaact ctaatgtga 
      gtgtggagt gccgtagta aagcgcgag gactaactc 
      aaattctca attcgaaga attgagcag tttgttgtt 
      gtgttttgt gaattttgg tttggtttt gtgacagtt 
      gggctaaca atactcgaa atgaattcc tatgcagat 
      aatatcaca gcaagcttg gaaaacagc agaaacagc 
      cttcggcgc atcgcccgc aacgacgac acggaattc 
      tcgcaccag agcattttg aacctgatg gagaagttc 
      gtgaagacg gtgaacgcg atggacgag acaatccta 
      gtgccttgc cgcctgatg gacctgaag gtgggggac 
      gagaacgac ccgacgtgt cccaaaaag cataaccaa 
      aagtcaaaa cacggtgtc caagagctg ctgagttcc 
      gccgatctg ttccaaatc tacaacatg ctgaacagc 
      gtgaaggcc gatttgctg tggggacaa ggacaggcg 
      gccgaggag gctccggct ccggccccg gcggcgaca 
      gtggtcaag ggacacgtg cgaagaccg tcgactgtc 
      agcgtagcc tcgacgaat tcgtcggcg tcgacgatc 
      agcgactcc gagtcggac gccgggaac gagaacgac 
      tccggaatc gaggaatcg caggagtgt ttcggggat 
      cggacggaa caagtcgcg caaaacttc aggaggcat 
      ctggtggga ctcacccgg tcgctgaaa cagatgacc 
      gaagctgcc cagtacctc acttcgcgt tatcagcac 
      gatatcgga gggccagtg tgacaaaga cctcttcga 
      agctcgagg gggagggtg tgcgtgggg gatgcgtga 
      gtggatgca tggatgcgt ggactgtat ttaaaagag 
      tgatattct caacgtttg tgttgctcc ttgattgtt 
      attaattat tatatttat tttagtgag agttacttg 
      tatacctct ggataagta aaggaaatt aaatacatt 
      atttaattt aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY