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Gene information
General information
TC001594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1: 10866..17081
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001594
    iBeetle screen iB_00267
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001594 RA
      MSGDVQPRK RKDKKRKKE ESTEHVPLQ PQTPSGAQN 
      EDLNIHVHK EDGTGGGIC AKIVFFLLF SALAVLIGL 
      IITEHRGLT DLDVVETES RFSHLFEGW VDNSLKHDD 
      HGDDDHIVS SVEEEEEEE EEDHDEVSH EDEEEEEEA 
      EASEEVTHS EEETPQTEE DEEEEEEEA EDNESGLKD 
      DDEEEADED DEEQDVEKS VEEEEEQED EDEEEEERV 
      VSEEKASEE VKEEEEEEE EAAEEPEVA EEKEEEEEE 
      EDATGDEQN SKMVVKIGV GVALLVVAH VVLVRKWKS 
      ASPSTETPP KDELPDLSR RNTIVVPPK YEEIEQDIE 
      ENVEEEYSD EDDEVESED EEKLAKVKY QELRTTYTR 
      SLTPESEPE ETKEEEYED EEEEEEEEV EDEEDEEES 
      EEDDDDLLK RLEAKYGKL QREDKEDEQ FSSWKRVNV 
      REDNEPEES DSDKQSDYE YENITSKDD YPIKEKLDE 
      AQKNIQNNT AYALKLFDA LLQNYSSSP RSLYGKAQA 
      LDVLAEQKR SNELLQKAI EAYLKVLDL DQVPDILFE 
      AAAERCINR MRFIGNYNR AIEVHYKLI ARFPQKTHH 
      RNQLVVSLL TINRVSKAR EILQETLSK NPNDGFALV 
      HYGFILKTV DNNLNESIS YLERGLSTK EPGVVDGRF 
      YFHLGDALS RVGRTLDAM KVYEEGVRN KLFLSKYQR 
      SLYNVARLK GQPWWKKEE TPYAALFQA LEANWKQIR 
      AEGVSVLSK DGHFQAESE NLKDTGDWK QFELFARGH 
      KNANNCRKC PLTCKIIES VPDAKGCRR GQTKFSVMH 
      PGTHVWPHC GPTNCRLRV HLGLQVPAN TFIRVAEKT 
      RSWKEGEVL IFDDSFEHE VWHNGTSLR LVLIVDVWH 
      PDLTPGEKR TLSPI
    CDS sequence
    • >TC001594 RA
      atgtcgggc gatgttcaa ccaagaaaa cgcaaagat 
      aaaaagaga aagaaagaa gaaagcacc gaacatgtg 
      ccgttgcaa ccccagaca ccttctggc gcacaaaac 
      gaagatctt aatattcat gtacataaa gaagacgga 
      acaggtggt ggcatttgc gcgaaaata gtcttcttt 
      ctacttttc tcagctctt gcggttctt atcggcttg 
      attataacc gaacatcgc ggattaaca gacttggat 
      gtagtggaa accgaatcc aggttttca catttgttt 
      gaaggctgg gttgataat agtttaaag catgatgat 
      catggtgat gacgatcat atcgtcagt tctgtagaa 
      gaagaagaa gaagaagaa gaagaagat cacgacgag 
      gtttcacac gaagacgag gaagaggaa gaagaagct 
      gaggcttcg gaagaagta actcattcg gaagaagag 
      acgccccaa actgaagaa gacgaagag gaggaagaa 
      gaagaagca gaggataac gaaagcggg ctaaaagac 
      gatgatgag gaggaagcc gacgaagat gatgaggaa 
      caggatgtt gaaaaaagc gtagaagag gaggaggaa 
      caagaggat gaagacgag gaagaagag gaaagagtt 
      gtatccgaa gagaaggcc agtgaggaa gtcaaagaa 
      gaagaagaa gaggaggaa gaagctgcc gaagagcct 
      gaagttgcc gaagagaaa gaagaagaa gaagaagaa 
      gaagacgcc accggagat gagcagaat tctaaaatg 
      gttgtaaag attggcgtc ggcgtggct cttttggta 
      gtagcccac gtcgttctg gttagaaag tggaagtct 
      gcaagtcca tcaactgaa acgccccca aaagacgaa 
      cttccagat ctcagccgc agaaacaca atcgtcgtg 
      cccccgaaa tacgaagag atagagcaa gacattgaa 
      gaaaatgtc gaagaagaa tattcggac gaagacgac 
      gaggttgaa agtgaagac gaggagaaa ctggctaaa 
      gtcaagtac caagagttg cggacgact tataccaga 
      tccctaaca ccggagagc gaacccgag gaaactaaa 
      gaggaggag tatgaagat gaggaggag gaggaagag 
      gaagaggtc gaggacgaa gaagatgag gaagaatcg 
      gaggaagac gatgacgat ctgttaaaa agactggaa 
      gccaagtac ggaaaattg cagcgtgaa gacaaggaa 
      gatgagcag ttttcgtcc tggaaacgt gtgaacgtc 
      cgtgaagac aatgaacct gaagaatcc gactctgat 
      aaacaatca gactacgag tacgaaaac ataacaagc 
      aaagacgac tacccgatc aaggaaaaa ctcgacgag 
      gctcagaaa aacatccaa aataatacg gcttatgca 
      cttaagctt tttgacgcc ctcctccaa aactattcc 
      tcttcaccc agaagtctc tacgggaaa gcccaagct 
      ctggacgtt ttagctgaa cagaaacgc agtaatgaa 
      ttgttacaa aaggcaatt gaagcttac ttgaaagtc 
      ctggacctt gatcaggtc ccagacatt ttattcgaa 
      gccgcagct gagaggtgt atcaatcgg atgagattt 
      ataggtaac tacaaccgt gctattgag gttcattac 
      aagttaatt gctcggttt ccgcaaaaa acgcaccat 
      cggaatcaa ttagtggtc agtttattg acaataaac 
      cgggtgagc aaggctcgg gaaattcta caagagaca 
      ctctctaaa aacccgaac gatggtttc gctcttgtc 
      cattacggg ttcattctt aaaacagtt gataataac 
      ttgaacgag tctataagt tatctcgaa aggggttta 
      tccacaaag gagcctggg gtggtcgat ggccgcttt 
      tacttccac ctaggggat gcactatct cgtgttggg 
      cgcactcta gatgccatg aaagtgtac gaagaaggc 
      gttcgtaac aagcttttc ctatccaag taccaacgt 
      tccctctac aatgtggca cgtctcaag ggccagcct 
      tggtggaaa aaggaagag acgccctac gcggccctt 
      ttccaagcg ttggaagcc aactggaag caaatccga 
      gccgagggc gtttccgtt ttgagcaaa gacggccac 
      ttccaagcc gaatcggaa aatttgaaa gacacgggc 
      gattggaaa cagttcgag ctgttcgca agagggcac 
      aaaaacgcg aacaattgc agaaagtgc ccccttacg 
      tgcaaaatc atcgaaagc gtccccgac gccaaaggg 
      tgcaggagg gggcaaacc aagttcagt gtgatgcat 
      ccgggcacc cacgtgtgg cctcactgt ggaccaaca 
      aactgtagg ttgagagta catttagga cttcaggtt 
      ccggcgaac acctttatc cgggttgct gagaagacc 
      aggagttgg aaggaggga gaagtgctc attttcgac 
      gacagtttc gagcacgaa gtgtggcac aacgggact 
      tcgctccga cttgtccta attgtggac gtgtggcac 
      ccggactta acaccgggc gaaaaacgg actctctcg 
      ccgatttga
    mRNA sequence
    • >TC001594 RA
      caccgcagc agatgcaac tattatcat tactaaata 
      taataaagt accgtgtaa tcggaactt gtcgtggac 
      tcccgacgt cgtttttgg ttctaaata aaactcgta 
      cgtttccta cctcttatc gcatggtga aaataccct 
      tccgaaata atttgcact caaagagga ggttttatg 
      gtggtgtgg tctaaatac tagacgata aacctgaca 
      cgaaaatgt cgggcgatg ttcaaccaa gaaaacgca 
      aagataaaa agagaaaga aagaagaaa gcaccgaac 
      atgtgccgt tgcaacccc agacacctt ctggcgcac 
      aaaacgaag atcttaata ttcatgtac ataaagaag 
      acggaacag gtggtggca tttgcgcga aaatagtct 
      tctttctac ttttctcag ctcttgcgg ttcttatcg 
      gcttgatta taaccgaac atcgcggat taacagact 
      tggatgtag tggaaaccg aatccaggt tttcacatt 
      tgtttgaag gctgggttg ataatagtt taaagcatg 
      atgatcatg gtgatgacg atcatatcg tcagttctg 
      tagaagaag aagaagaag aagaagaag aagatcacg 
      acgaggttt cacacgaag acgaggaag aggaagaag 
      aagctgagg cttcggaag aagtaactc attcggaag 
      aagagacgc cccaaactg aagaagacg aagaggagg 
      aagaagaag aagcagagg ataacgaaa gcgggctaa 
      aagacgatg atgaggagg aagccgacg aagatgatg 
      aggaacagg atgttgaaa aaagcgtag aagaggagg 
      aggaacaag aggatgaag acgaggaag aagaggaaa 
      gagttgtat ccgaagaga aggccagtg aggaagtca 
      aagaagaag aagaagagg aggaagaag ctgccgaag 
      agcctgaag ttgccgaag agaaagaag aagaagaag 
      aagaagaag acgccaccg gagatgagc agaattcta 
      aaatggttg taaagattg gcgtcggcg tggctcttt 
      tggtagtag cccacgtcg ttctggtta gaaagtgga 
      agtctgcaa gtccatcaa ctgaaacgc ccccaaaag 
      acgaacttc cagatctca gccgcagaa acacaatcg 
      tcgtgcccc cgaaatacg aagagatag agcaagaca 
      ttgaagaaa atgtcgaag aagaatatt cggacgaag 
      acgacgagg ttgaaagtg aagacgagg agaaactgg 
      ctaaagtca agtaccaag agttgcgga cgacttata 
      ccagatccc taacaccgg agagcgaac ccgaggaaa 
      ctaaagagg aggagtatg aagatgagg aggaggagg 
      aagaggaag aggtcgagg acgaagaag atgaggaag 
      aatcggagg aagacgatg acgatctgt taaaaagac 
      tggaagcca agtacggaa aattgcagc gtgaagaca 
      aggaagatg agcagtttt cgtcctgga aacgtgtga 
      acgtccgtg aagacaatg aacctgaag aatccgact 
      ctgataaac aatcagact acgagtacg aaaacataa 
      caagcaaag acgactacc cgatcaagg aaaaactcg 
      acgaggctc agaaaaaca tccaaaata atacggctt 
      atgcactta agctttttg acgccctcc tccaaaact 
      attcctctt cacccagaa gtctctacg ggaaagccc 
      aagctctgg acgttttag ctgaacaga aacgcagta 
      atgaattgt tacaaaagg caattgaag cttacttga 
      aagtcctgg accttgatc aggtcccag acattttat 
      tcgaagccg cagctgaga ggtgtatca atcggatga 
      gatttatag gtaactaca accgtgcta ttgaggttc 
      attacaagt taattgctc ggtttccgc aaaaaacgc 
      accatcgga atcaattag tggtcagtt tattgacaa 
      taaaccggg tgagcaagg ctcgggaaa ttctacaag 
      agacactct ctaaaaacc cgaacgatg gtttcgctc 
      ttgtccatt acgggttca ttcttaaaa cagttgata 
      ataacttga acgagtcta taagttatc tcgaaaggg 
      gtttatcca caaaggagc ctggggtgg tcgatggcc 
      gcttttact tccacctag gggatgcac tatctcgtg 
      ttgggcgca ctctagatg ccatgaaag tgtacgaag 
      aaggcgttc gtaacaagc ttttcctat ccaagtacc 
      aacgttccc tctacaatg tggcacgtc tcaagggcc 
      agccttggt ggaaaaagg aagagacgc cctacgcgg 
      cccttttcc aagcgttgg aagccaact ggaagcaaa 
      tccgagccg agggcgttt ccgttttga gcaaagacg 
      gccacttcc aagccgaat cggaaaatt tgaaagaca 
      cgggcgatt ggaaacagt tcgagctgt tcgcaagag 
      ggcacaaaa acgcgaaca attgcagaa agtgccccc 
      ttacgtgca aaatcatcg aaagcgtcc ccgacgcca 
      aagggtgca ggagggggc aaaccaagt tcagtgtga 
      tgcatccgg gcacccacg tgtggcctc actgtggac 
      caacaaact gtaggttga gagtacatt taggacttc 
      aggttccgg cgaacacct ttatccggg ttgctgaga 
      agaccagga gttggaagg agggagaag tgctcattt 
      tcgacgaca gtttcgagc acgaagtgt ggcacaacg 
      ggacttcgc tccgacttg tcctaattg tggacgtgt 
      ggcacccgg acttaacac cgggcgaaa aacggactc 
      tctcgccga tttgatgta ggttttgta caaaaataa 
      agtttcaga aaaatg