Status
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Gene information
General information
TC001579 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3102129..3143883
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001579
    iBeetle screen iB_00266

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001579 RA
      MVIVDKRVQ CGVNYYCVL DYSATLGNL TCPRKENRN 
      VRCLERRLL ARVVLGKWT SMKTFRKQL LTVLLLLHA 
      GVNEAVDLS QCIAPLGME NGAIKDEDI TASSSFDSG 
      NVGPHHGRV RTDRNGGAW CPLKQATTE PEEWIQIDL 
      KTVHMITAT GSQGRFGNG VGIEYAEAY MLEYWRPKL 
      SKWIRYHNS KGEEVLPGN INTYLESKN TLVPPIWVS 
      KIRFYPYSY HKRTVCMRV ELYGCRYTD GIVSYSMPQ 
      GDKRGSNWE FYDFGYDGH WDGDELKHG LGQLIDGIF 
      GHDDFKTDF YDEAQTWVG WKNDTRQHK PIEIKFEFD 
      RVREFSAVH IYCNNQFTK DVQIFSHAK VMFSVGGKR 
      FKGEPITYE YMEDRIFEN ARNVTIKLH HRVGRFVKV 
      QLHFAAKWL LVSEITFDS VVAHGNFTV ESEPKAAPP 
      TQGHELHEQ KIEIPVPNK INEPAYLTI IVIGLAFII 
      LLLGGVIYL IIHRFRNRK FFRSPNSSN IGFPGDTLP 
      HLQPESVLG MSEKGSSIE AYGVTEIED YRRSHGTLK 
      SSLRSTLPL PHPGHMSEG NEYQEPYQA LKYAPYYSY 
      SSVVMEMQD AMPNSIKKC PIQQIDAYD YAVPEPGTL 
      PLLGSDNTL PINRSRMSS VSLKSQKMN ARNSHSEGK 
      RSPTQQEAL TALKKRLED TQLVEFPRH RLRMLSKLS 
      EGAFGTVYI AEADGITEF SSSMSLGTR LVAIKFLGE 
      NAAEKEKKD FYRDVRILA ALEDPNIAR VLGICSQEE 
      PICVVMEYL DHGDLCQFL KTHVAADNN ASLPYGVKS 
      LSFNCLLYM GAQIASGMR YLESLNFVH RDLATRNCL 
      IGKGYQIKI CDFGTYNEL YINDYYKVD GNTPLPIRW 
      MAWESVYQT RYTTKSDVW AFAVTLWEI LTLCRRPPY 
      DSLPDPEVM ENLAHMHCD DGQYMYLPR PTASKDIYD 
      LMLECWRRK DVERPSFRE IHIFLQRKN LGYAPT
    CDS sequence
    • >TC001579 RA
      atggttatc gtggataag cgagttcag tgtggtgta 
      aattattat tgcgtgttg gattattct gctacatta 
      gggaatttg acatgtcca agaaaagaa aatagaaat 
      gtaagatgc cttgagaga cgattactc gcgagggtc 
      gtcctcggc aagtggaca agtatgaag acgttccgt 
      aagcagctc ctcacggtc cttctcctc cttcatgcc 
      ggcgttaac gaggctgtg gacctctcg caatgtatc 
      gctcctctg gggatggag aatggagca atcaaggac 
      gaggacatc accgcaagc tcgtcgttc gatagcggt 
      aacgtgggc cctcatcac ggccgggtg cgaacggac 
      cgaaacggg ggcgcttgg tgtccgctg aagcaggcg 
      acaacggag cccgaagaa tggatccaa atcgatttg 
      aaaacagtt catatgata acagcaacc ggaagccag 
      ggccgcttc ggtaacggg gttggaatt gaatacgcg 
      gaagcttac atgctggaa tattggcgg cccaagctc 
      tcgaaatgg atacgttat cataacagc aaaggcgag 
      gaggtcctg cctggcaac atcaacaca tatctggaa 
      tcgaagaac actctcgtg cctcccatc tgggtgagc 
      aaaatccgc ttctacccc tacagctac cacaaacgt 
      acagtttgc atgcgagtg gagctgtac ggctgccgc 
      tacaccgac ggcatcgtc agctactcc atgccccag 
      ggcgacaaa cgcggctcc aactgggaa ttctacgac 
      ttcggctac gacggccac tgggacggc gacgagcta 
      aagcacggc ctgggccag ctaatcgac ggaatcttc 
      ggccacgac gacttcaaa accgacttc tacgacgag 
      gcccagacc tgggtcggc tggaagaac gacacgcga 
      cagcacaaa cccatcgaa atcaagttc gagttcgac 
      cgcgtgcgc gaattctcc gccgtccac atctactgc 
      aacaatcag tttacgaaa gacgtccag attttctcg 
      cacgccaaa gtcatgttc agcgtcgga ggcaagcgc 
      ttcaaaggc gaacccatc acgtacgag tacatggag 
      gaccgcatc ttcgaaaac gcgcgaaat gtcaccata 
      aagctccac catagagtg gggcggttt gttaaagtg 
      cagctccac ttcgccgcc aagtggctt ctcgtcagc 
      gagatcacc ttcgactcg gtggtggcg cacggcaac 
      ttcaccgtg gagtcggag ccgaaggcc gcgccgccc 
      acccaaggg cacgagctc cacgagcaa aagatcgag 
      attcccgtg ccgaataag ataaatgaa ccggcttat 
      ttgacaatc atcgtgatc gggctagcg tttattata 
      ctgttgttg ggcggagtg atttacctg atcatccac 
      cgcttccgg aatagaaag ttcttccgc agtccgaac 
      tcctccaac attgggttt ccgggcgat actttgccg 
      catcttcag ccggagtcg gtgctgggg atgagcgag 
      aaagggagc tcgattgag gcgtatgga gttactgaa 
      attgaggat taccgcaga agtcatggc actttgaag 
      tcgagcttg cggtctact ctaccactg ccacatcct 
      ggacacatg tcggaaggc aacgaatat caagaacca 
      tatcaagcg ctcaaatat gcgccttat tatagctac 
      agctcagtg gttatggaa atgcaggat gctatgccc 
      aacagtatt aagaaatgc ccaatccag caaatagat 
      gcttatgat tacgcagta cctgagcct gggactctt 
      ccgcttctt ggttcggac aacacattg ccaataaat 
      agaagtaga atgagtagc gtctcttta aaaagccag 
      aaaatgaat gcgagaaac agccacagt gaaggtaaa 
      aggtcccca acgcaacaa gaagctcta accgcgcta 
      aaaaagcgc ttagaagac acacaactt gtagagttt 
      ccaaggcat cgattgcgg atgctatcg aaactttct 
      gaaggagca tttggaacg gtatacatc gctgaggcg 
      gacgggata accgaattc tcctcgtcc atgtctttg 
      gggacgcgc ctcgtggca ataaaattc ctcggcgaa 
      aacgccgcc gaaaaggag aagaaggat ttctaccgc 
      gacgtgcga atcctggcg gctctggag gacccaaac 
      atcgcccgt gtcctgggg atttgcagc caagaggag 
      ccgatttgt gtggtgatg gagtatttg gaccacggt 
      gatctgtgt cagttctta aaaacgcac gttgccgcg 
      gacaacaat gccagttta ccgtacggt gttaagtcg 
      ctctcgttc aactgcctt ctgtacatg ggggcccag 
      atcgcctcg ggcatgcgc tatttggag tccctcaac 
      ttcgtgcat cgcgacctg gcgaccagg aactgcctc 
      ataggcaag ggatatcag atcaagatc tgcgatttc 
      ggtacttac aacgaactt tatatcaat gactactac 
      aaggtggac ggcaacacg ccgctgccc atccgatgg 
      atggcctgg gagtcggtc taccagacc aggtacaca 
      acgaaaagc gacgtgtgg gcgtttgct gtcaccttg 
      tgggaaatt ctgacgctg tgtcggaga ccgccttac 
      gattcttta ccggatcct gaagttatg gagaattta 
      gcacacatg cactgtgat gatggacag tatatgtat 
      ctacctcga cccacggct agcaaggat atttacgac 
      ttgatgctc gagtgctgg agacggaaa gatgtggag 
      cggccgagc tttagagag attcacatt ttcttgcaa 
      aggaaaaac ttaggatac gctcctaca tag
    mRNA sequence
    • >TC001579 RA
      cgtctgtgg aggaggatt agttcaaca tgtttctga 
      ctttgaaca cgcgtatat gtgtatgta atccacaat 
      aaacattaa ggcccccgt gtctgaccc cgataaaat 
      tagccggta cattgtgga gccaagttc gaattttga 
      atttgttgt tgtaaaagg gacgtttaa ttatgtgga 
      agttctaaa tggttatcg tggataagc gagttcagt 
      gtggtgtaa attattatt gcgtgttgg attattctg 
      ctacattag ggaatttga catgtccaa gaaaagaaa 
      atagaaatg taagatgcc ttgagagac gattactcg 
      cgagggtcg tcctcggca agtggacaa gtatgaaga 
      cgttccgta agcagctcc tcacggtcc ttctcctcc 
      ttcatgccg gcgttaacg aggctgtgg acctctcgc 
      aatgtatcg ctcctctgg ggatggaga atggagcaa 
      tcaaggacg aggacatca ccgcaagct cgtcgttcg 
      atagcggta acgtgggcc ctcatcacg gccgggtgc 
      gaacggacc gaaacgggg gcgcttggt gtccgctga 
      agcaggcga caacggagc ccgaagaat ggatccaaa 
      tcgatttga aaacagttc atatgataa cagcaaccg 
      gaagccagg gccgcttcg gtaacgggg ttggaattg 
      aatacgcgg aagcttaca tgctggaat attggcggc 
      ccaagctct cgaaatgga tacgttatc ataacagca 
      aaggcgagg aggtcctgc ctggcaaca tcaacacat 
      atctggaat cgaagaaca ctctcgtgc ctcccatct 
      gggtgagca aaatccgct tctacccct acagctacc 
      acaaacgta cagtttgca tgcgagtgg agctgtacg 
      gctgccgct acaccgacg gcatcgtca gctactcca 
      tgccccagg gcgacaaac gcggctcca actgggaat 
      tctacgact tcggctacg acggccact gggacggcg 
      acgagctaa agcacggcc tgggccagc taatcgacg 
      gaatcttcg gccacgacg acttcaaaa ccgacttct 
      acgacgagg cccagacct gggtcggct ggaagaacg 
      acacgcgac agcacaaac ccatcgaaa tcaagttcg 
      agttcgacc gcgtgcgcg aattctccg ccgtccaca 
      tctactgca acaatcagt ttacgaaag acgtccaga 
      ttttctcgc acgccaaag tcatgttca gcgtcggag 
      gcaagcgct tcaaaggcg aacccatca cgtacgagt 
      acatggagg accgcatct tcgaaaacg cgcgaaatg 
      tcaccataa agctccacc atagagtgg ggcggtttg 
      ttaaagtgc agctccact tcgccgcca agtggcttc 
      tcgtcagcg agatcacct tcgactcgg tggtggcgc 
      acggcaact tcaccgtgg agtcggagc cgaaggccg 
      cgccgccca cccaagggc acgagctcc acgagcaaa 
      agatcgaga ttcccgtgc cgaataaga taaatgaac 
      cggcttatt tgacaatca tcgtgatcg ggctagcgt 
      ttattatac tgttgttgg gcggagtga tttacctga 
      tcatccacc gcttccgga atagaaagt tcttccgca 
      gtccgaact cctccaaca ttgggtttc cgggcgata 
      ctttgccgc atcttcagc cggagtcgg tgctgggga 
      tgagcgaga aagggagct cgattgagg cgtatggag 
      ttactgaaa ttgaggatt accgcagaa gtcatggca 
      ctttgaagt cgagcttgc ggtctactc taccactgc 
      cacatcctg gacacatgt cggaaggca acgaatatc 
      aagaaccat atcaagcgc tcaaatatg cgccttatt 
      atagctaca gctcagtgg ttatggaaa tgcaggatg 
      ctatgccca acagtatta agaaatgcc caatccagc 
      aaatagatg cttatgatt acgcagtac ctgagcctg 
      ggactcttc cgcttcttg gttcggaca acacattgc 
      caataaata gaagtagaa tgagtagcg tctctttaa 
      aaagccaga aaatgaatg cgagaaaca gccacagtg 
      aaggtaaaa ggtccccaa cgcaacaag aagctctaa 
      ccgcgctaa aaaagcgct tagaagaca cacaacttg 
      tagagtttc caaggcatc gattgcgga tgctatcga 
      aactttctg aaggagcat ttggaacgg tatacatcg 
      ctgaggcgg acgggataa ccgaattct cctcgtcca 
      tgtctttgg ggacgcgcc tcgtggcaa taaaattcc 
      tcggcgaaa acgccgccg aaaaggaga agaaggatt 
      tctaccgcg acgtgcgaa tcctggcgg ctctggagg 
      acccaaaca tcgcccgtg tcctgggga tttgcagcc 
      aagaggagc cgatttgtg tggtgatgg agtatttgg 
      accacggtg atctgtgtc agttcttaa aaacgcacg 
      ttgccgcgg acaacaatg ccagtttac cgtacggtg 
      ttaagtcgc tctcgttca actgccttc tgtacatgg 
      gggcccaga tcgcctcgg gcatgcgct atttggagt 
      ccctcaact tcgtgcatc gcgacctgg cgaccagga 
      actgcctca taggcaagg gatatcaga tcaagatct 
      gcgatttcg gtacttaca acgaacttt atatcaatg 
      actactaca aggtggacg gcaacacgc cgctgccca 
      tccgatgga tggcctggg agtcggtct accagacca 
      ggtacacaa cgaaaagcg acgtgtggg cgtttgctg 
      tcaccttgt gggaaattc tgacgctgt gtcggagac 
      cgccttacg attctttac cggatcctg aagttatgg 
      agaatttag cacacatgc actgtgatg atggacagt 
      atatgtatc tacctcgac ccacggcta gcaaggata 
      tttacgact tgatgctcg agtgctgga gacggaaag 
      atgtggagc ggccgagct ttagagaga ttcacattt 
      tcttgcaaa ggaaaaact taggatacg ctcctacat 
      agtttagga agactggtt aggtataga gggatcaac 
      atttatgta tatagaaca cagggtgat aatatctat 
      ttttatttc gactttgtt ttgtacaaa ctcagttag 
      gcttattct ttgacaagt gtttttatt aacagattt 
      atcacacgc gacacggag gactagaac taggaggcg 
      aataacgta ctcggcaaa tacaaacgt gggacattt 
      ctaaaattg taagaaaat tgcatttag aacaacaac 
      acaaagtta gtacatgtt atatgaact ttttgacaa 
      ttttcaaat aaaaaaatc aaacattta gtgaaaaat 
      tataattca atgataagg cttcctaat attttagtc 
      cttcgtgta cgcctttcg ggtaaaaac gattcacgt 
      tgtttgttt tctatttac gtggaagaa gagtaaaaa 
      ttatagaaa tttatgagt gaatctatt ttagcgtct 
      gttaatacg aacaagtgt acctcccat gctttaccc 
      atttgttat ataaactct tcacaaggc acaataatt 
      caagacaat gtatagata atattgtaa aagtaataa 
      tatcagtgt tatataatt atatttact agtcaattg 
      aggcgccta agtaaaata ttttcaacg acttaatga 
      tgtcacaaa ggagcttat gatggaaag taacgaaaa 
      aagcattct aaactatat atcttctac attagatag 
      tattttaac gatatgcgc tcctgtgct ttacaagtg 
      atgtctata atatataga aatgaaact tttctagtt 
      accaaaaat attgtaacg ttgtttcag ttgtatggt 
      ttttatact tgctgatga aaagtgtat gctcggaga 
      tgtacctac aataacttt taataacag tcaggtttt 
      agttcatta taatggatt tgattattg tttaatgtg 
      ccaataagc ttcacttac agtatagcg aattgttat 
      ttattttag ctgtaattt attttgctt cattgtaca 
      tgtaaaact caactatta gatactcta tgtttctta 
      atgacatac ttactaaag tattcaaac attccaaca 
      taatataag tggcacaaa tttttaata acttttgca 
      ataatgaac catttgtga ttttgttga tgtgcattt 
      aaattctgc aattaataa ttatgtcga gtaataata 
      acatatcaa tttaataaa ttgtaatta ggtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY