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Gene information
General information
TC001570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3576570..3578666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001570
    iBeetle screen iB_00264

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001570 RA
      MVKPVRKNS NKNPPILSH EFIIQNHAD IVSCVAMVF 
      VVGLMVQAT SPLASVFIA LQHNVTEAG EIPLYSPGI 
      KDWAAVFFY SLICIVIHA IIQEYGLDK ISKKLHLSK 
      SKLAVFSTS GQLTVFYLI STFWGADII LKEQYLPEI 
      ARLWSDYPA PMTFLVKLY IIIQLAYSL HELPELYFQ 
      RVKREEYSS KAVQSVATL LLVAVPYFL NFNRLLVCL 
      LVLHHSSEL FYHITQLIQ TIDKDEKFS KAARMVSNI 
      LQISSRLGS IILAVLTLW YGLALGEQQ PLDIQAGYY 
      NTATVRLAV LGGILVLQM YLTINVINQ EIARSRENK 
      AYTTVPKPK VQKKEKSKK SKKVNNEES DLPEVDQNT 
      NKTLRHKQK VK
    CDS sequence
    • >TC001570 RA
      atggtcaaa ccagtcaga aaaaactca aacaagaac 
      cccccaatc ttgagccac gaattcatc atccaaaac 
      catgcggac atcgtctcc tgtgtggct atggtgttc 
      gtggtgggc cttatggtc caagccaca tcgccccta 
      gcttcagtc ttcattgcg ctccaacac aacgtaaca 
      gaagctggg gagattcct ttatacagt cccgggatt 
      aaagactgg gcagctgtt ttcttctac agcttgatc 
      tgtatcgtg attcacgcc attatccaa gaatatgga 
      cttgataaa atctcgaag aagctgcat ttgtccaag 
      tcgaagctt gcggttttt tcgaccagt ggccaattg 
      accgtgttt tatctaatt tcgacattc tggggggct 
      gatattatc ttgaaggag caatatttg cccgaaatt 
      gcacgactt tggtccgac tatcccgct ccaatgact 
      ttcctcgtt aaattatac attatcata caacttgcc 
      tacagtctg cacgaatta cctgaactg tacttccaa 
      cgagtgaag cgcgaagaa tattcatca aaagccgtc 
      caaagcgtt gcaactctg cttcttgta gctgtcccc 
      tacttctta aacttcaat cgcctgctt gtttgcctc 
      ctggtgctc caccatagc tccgaattg ttttaccac 
      atcacccaa ctgatccaa acaattgac aaagatgag 
      aaattttca aaggcggcc agaatggtt tccaacatt 
      ttacagatt tcgtcccgt ttgggtagc attatcttg 
      gctgtttta accctgtgg tatggcttg gctttagga 
      gagcaacaa ccccttgac atccaagcc ggttactac 
      aacactgct actgtccgt cttgctgtt ttgggagga 
      atccttgtt ttgcaaatg tatctgacc attaacgta 
      atcaatcaa gaaattgca cgttcgcgc gaaaataaa 
      gcctacacc actgttccc aaacccaaa gtgcagaag 
      aaagaaaaa tcgaaaaaa agtaaaaaa gttaataac 
      gaggaatct gatctcccc gaagtggat caaaacaca 
      aataagact ttaagacac aaacagaaa gtaaagtaa
    mRNA sequence
    • >TC001570 RA
      gattttata ataattttg ttttattac aattttaca 
      aattccagt gttgaaacg tgaacagaa aattgctgt 
      ttcaaactt tggcggcaa tcctttcac tcgcaactg 
      acatttgac agttgacaa ttgttgtaa ttgtcaaaa 
      ttgcggctg gtgttttag tggctacgt aaaatttat 
      cgacgtcga cgattacgt tgtctacgt gtctgtgta 
      ccacttcga gaacctcca aaaccagcc aaaacatac 
      gttcactct gtgtcgtgg aagcagcgg tgtcggtgc 
      gttgtagtg gtgctaaca gtacaagaa cacagaaag 
      aagaccaga ccgttacag ttacactac cgttattaa 
      aagaatagt ctatttaac gcaacttta attagtaaa 
      taactttaa tcgcgcaaa gatggtcaa accagtcag 
      aaaaaactc aaacaagaa ccccccaat cttgagcca 
      cgaattcat catccaaaa ccatgcgga catcgtctc 
      ctgtgtggc tatggtgtt cgtggtggg ccttatggt 
      ccaagccac atcgcccct agcttcagt cttcattgc 
      gctccaaca caacgtaac agaagctgg ggagattcc 
      tttatacag tcccgggat taaagactg ggcagctgt 
      tttcttcta cagcttgat ctgtatcgt gattcacgc 
      cattatcca agaatatgg acttgataa aatctcgaa 
      gaagctgca tttgtccaa gtcgaagct tgcggtttt 
      ttcgaccag tggccaatt gaccgtgtt ttatctaat 
      ttcgacatt ctggggggc tgatattat cttgaagga 
      gcaatattt gcccgaaat tgcacgact ttggtccga 
      ctatcccgc tccaatgac tttcctcgt taaattata 
      cattatcat acaacttgc ctacagtct gcacgaatt 
      acctgaact gtacttcca acgagtgaa gcgcgaaga 
      atattcatc aaaagccgt ccaaagcgt tgcaactct 
      gcttcttgt agctgtccc ctacttctt aaacttcaa 
      tcgcctgct tgtttgcct cctggtgct ccaccatag 
      ctccgaatt gttttacca catcaccca actgatcca 
      aacaattga caaagatga gaaattttc aaaggcggc 
      cagaatggt ttccaacat tttacagat ttcgtcccg 
      tttgggtag cattatctt ggctgtttt aaccctgtg 
      gtatggctt ggctttagg agagcaaca accccttga 
      catccaagc cggttacta caacactgc tactgtccg 
      tcttgctgt tttgggagg aatccttgt tttgcaaat 
      gtatctgac cattaacgt aatcaatca agaaattgc 
      acgttcgcg cgaaaataa agcctacac cactgttcc 
      caaacccaa agtgcagaa gaaagaaaa atcgaaaaa 
      aagtaaaaa agttaataa cgaggaatc tgatctccc 
      cgaagtgga tcaaaacac aaataagac tttaagaca 
      caaacagaa agtaaagta agttgtttc taagatttc 
      tttttttat atatacatg cattatttt gtaaaacca 
      gactttact gctggggac tatttctga attgtttgt 
      tttctgtaa aaataaata tgatttttt gat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY