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Gene information
General information
TC001569 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2500251..2502805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001569
      iBeetle screen iB_00263
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001569 RA
        MGLRNTLIF VALVSCVLA YSGEFVPKS 
        YYIIDQDGH KSDVVYFRS KRDVESFPL 
        FRVKRGGGS SSSSQSSAS SSSSSGAGF 
        GAGGGGAGG GGIPFQGGS DYFPGFIDP 
        GFAEFFNSN FDALAKLAS STGGGGGGG 
        SGAFAHSFA SSSSSGSSG IPSGFGGVG 
        GTAGPGYKG PILFSRFGE DTGNGVKVS 
        SSAQGPRGA FSSSSSSID SDGKIKYSV 
        KSGKY
      CDS sequence
      • >TC001569 RA
        atgggtctg cgaaacact ttaattttt gtggcttta 
        gttagttgt gtcttagct tattcaggc gaattcgtc 
        ccaaaatcc tactacata atcgaccag gatggtcac 
        aaatccgac gtcgtctac ttcaggagt aaaagggac 
        gtggaatcg ttccccctt ttccgggta aaacgtggg 
        ggcggctcc tcatcctct tcgcaaagt tccgcgtct 
        agcagcagc agcagcggc gccggattc ggggctggg 
        ggcggcggc gccggaggc ggtggcata ccgttccag 
        ggaggcagc gactacttt ccgggattc atagaccct 
        ggattcgct gaatttttc aacagcaac ttcgatgcc 
        ttagcaaaa cttgccagt agtaccggt ggcggcggc 
        ggcggcgga agcggtgca tttgcacat tctttcgcc 
        agttccagt tcgagcggc tcttcgggg attccctca 
        ggttttgga ggtgttggc ggaacggcg ggacccgga 
        tataaagga ccaatttta ttttctcgc tttggtgaa 
        gatactggt aatggtgtt aaagtttca agtagcgct 
        caaggaccc agaggagcc tttagttcg agttcgtct 
        tcaattgac agtgatgga aaaataaaa tattctgtg 
        aaatctgga aaatactag
      mRNA sequence
      • >TC001569 RA
        aagtttaaa catttcaag atcgacacc ggtacatgc 
        cggtactaa aatgtattt tacaatggg tctgcgaaa 
        cactttaat ttttgtggc tttagttag ttgtgtctt 
        agcttattc aggcgaatt cgtcccaaa atcctacta 
        cataatcga ccaggatgg tcacaaatc cgacgtcgt 
        ctacttcag gagtaaaag ggacgtgga atcgttccc 
        ccttttccg ggtaaaacg tgggggcgg ctcctcatc 
        ctcttcgca aagttccgc gtctagcag cagcagcag 
        cggcgccgg attcggggc tgggggcgg cggcgccgg 
        aggcggtgg cataccgtt ccagggagg cagcgacta 
        ctttccggg attcataga ccctggatt cgctgaatt 
        tttcaacag caacttcga tgccttagc aaaacttgc 
        cagtagtac cggtggcgg cggcggcgg cggaagcgg 
        tgcatttgc acattcttt cgccagttc cagttcgag 
        cggctcttc ggggattcc ctcaggttt tggaggtgt 
        tggcggaac ggcgggacc cggatataa aggaccaat 
        tttattttc tcgctttgg tgaagatac tggtaatgg 
        tgttaaagt ttcaagtag cgctcaagg acccagagg 
        agcctttag ttcgagttc gtcttcaat tgacagtga 
        tggaaaaat aaaatattc tgtgaaatc tggaaaata 
        ctagtactc gcatttatg taactttag tattaggga 
        ttatgtata cgtcttatg tcggtacaa taaagtata 
        aaaaatgat tcttagcca ggactgtga acacataca 
        tcattttat ttttatgtg gacacctaa cacctttag 
        atatttatg tttaagatg ttcagcata aaatcagtg 
        attttgtat tatttttat cacaatatt aggtttcct 
        gactatgtc aaaggacga aggtggtat tttagggca 
        aaaataagg aaaacgaca attttaaat tcgtcgcga 
        cgtttcgag ctttattgt atcttcagg cgagggatt 
        tttgcttga aaggtatgg cacagctaa ggaaaaaca 
        tgattatcg taagaactt ttattgaac aactaaaat 
        cgacccgtg agaagagcc tttcagatc catcataca 
        aaatttagt ttttatgca gttagtctc aagtgacct 
        gttttattt taaggtttg aagtggaat ttaataact 
        gttgttcct ctgcaatgc aggtattta cttcttgtt 
        taaagggca aaccctaaa acaattcta aagctacat 
        tcttttatg aagt