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Gene information
General information
TC001565 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3610202..3617666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001565
    iBeetle screen iB_00262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001565 RA
      MDMKNNLAL KKKRELLGG INKRKMTLR KYMDNLKQS 
      ECRLDITQI TKCVFLGSL CLKPVPHLF DSPLLLEEL 
      DAKVGSVGT EFEYIEMKS VMSDKEAVT KDSVKRGLK 
      KKTLNSPKI KKKTLKVKT DIRTTPNGY NSVKSESAS 
      ELVEATTAM SPVLPVINT EETSPVLSS FQTEPSESM 
      STSEENQVE TPFPDFLDI KLLNQNIKD IKGVNPDPF 
      AFEEDEAER LRREPTPEL ILSDSDEDA IDQLMYNIM 
      DIKVGPNVF DKDFFDMPP SDIEEDYEE YSNDSQENK 
      NEPNTVITR GKKYSKRAP QCKICSQVF STYMQLKKH 
      KLAHDGDKP YFCLKCNEN HQTLDQLIA HLRTHQGKR 
      PYVCKKCNQ GFKTLKGLE SHHPVHVLK KVSSQKRVF 
      RCEVCNKEF HKLCDLERH TRVHTGEKP SICNICNKR 
      FQQAHNLNK HLLIHTQEK PYECDVCHK KFGRNDVLN 
      RHLLTHSVK KPNRCEICM KSFIRVSQL LAHNKKHHT 
      EMAS
    CDS sequence
    • >TC001565 RA
      atggacatg aaaaacaat ttggcattg aagaaaaaa 
      cgggaacta cttgggggc atcaacaag cgaaaaatg 
      accctgagg aagtacatg gacaacctc aaacagagt 
      gagtgtcga ctggacatt acccagatc acaaagtgc 
      gtttttttg ggctctctg tgcctcaaa cccgtgccc 
      catcttttc gattcgccc ttgcttctg gaggagctt 
      gatgcgaaa gttggcagt gtggggact gaatttgag 
      tacattgaa atgaaatca gttatgagt gataaggaa 
      gctgttaca aaagactcc gttaaacgg gggcttaag 
      aaaaaaaca ctaaactcg ccgaagatt aaaaagaaa 
      acgctgaaa gtaaagact gacattaga acgactccc 
      aatggctac aacagtgtg aaaagcgaa agtgcaagt 
      gaacttgtt gaagccaca acagccatg agtcccgtg 
      cttccagta ataaacacc gaagaaact tccccagtc 
      ctgtcttca ttccaaacc gaaccgagc gaaagtatg 
      tccacaagt gaggaaaat caagtggaa acacccttc 
      cctgacttt ctagacatt aaactccta aaccagaac 
      atcaaagac attaagggc gtaaacccc gaccccttc 
      gccttcgaa gaagacgag gcagagcgc ctgagacgc 
      gagccaaca cctgaatta attttatcc gactctgat 
      gaagacgca attgaccaa ttaatgtac aacatcatg 
      gacattaaa gtgggacca aacgttttc gacaaagac 
      ttctttgac atgcccccc tctgacatt gaagaggac 
      tacgaagag tactcgaac gattcgcag gaaaataaa 
      aacgagcca aacaccgtc atcaccaga gggaaaaag 
      tacagcaaa cgcgccccc caatgcaag atctgttcg 
      caagtcttc tccacctat atgcagctc aaaaaacac 
      aaactggcc cacgatggc gacaaaccc tacttctgt 
      ctgaagtgc aacgaaaac caccagact ctcgaccag 
      ctcattgcg catctgagg acgcatcag gggaagcgg 
      ccctacgtg tgcaagaag tgcaaccag ggcttcaag 
      acgctcaag ggcttggag agccaccac ccggtgcac 
      gtgttgaag aaagtcagt tcgcagaag cgagtcttc 
      aggtgtgag gtttgtaac aaagagttc cacaagttg 
      tgcgatttg gaaagacat acgagagtg catacgggt 
      gaaaaaccg tcgatttgt aacatttgc aacaagcgt 
      ttccaacaa gcgcacaat ttaaataaa catttgctt 
      attcacacg caggaaaag ccgtatgag tgtgatgtt 
      tgtcacaag aagttcggc cggaacgat gttttaaac 
      agacatttg cttacgcat tcggttaaa aaaccgaac 
      cggtgcgag atttgtatg aagagtttc attcgggtg 
      tcgcagctt ctagctcat aataaaaaa caccataca 
      gaaatggct tcctag
    mRNA sequence
    • >TC001565 RA
      ccggattag cgcggctct gtgttatat taaatttaa 
      aaattgaac tgaattatg ccacagttt cagttaaat 
      ttaaagtat cttgaatct tgcgggatg ataggaaaa 
      aagccaccc agcacatga gtcttgaaa aaattgtaa 
      agtaaaatt gttgagaac tatatttct gggacagcc 
      tccataaag cggttcagt gtgagactt caaataatt 
      aaaatttct gtgcaggac gtggttatc cacttcctt 
      gggtatcca aattccgcg cctatttta ttagaactg 
      ttatttacc aaagtcgat gccgcgtat tttaatatt 
      acgtacaat gcgtgacat aattaatga cacatttgc 
      ggttcggaa agagtcact tgtagacaa ttcccctag 
      tttgaaagg aactataaa tagttaatc aatatggaa 
      atggatgcg gaacatgct tgacagacc gcgagaatt 
      ctgttcaag gcgatttta acactgtgt cattaattg 
      tctcatgca tgtgcggat tttatctat attttataa 
      ttattattg taaatcgca acactgcca ttaaattat 
      caatgcata cgatattgt attataaaa tttacaatg 
      tttacaata ttttttaca taattttca attgttttg 
      taaacacgg aaaaatttt ggcagaaac atgatagtg 
      aattttaat atttaccta atgactatc gagtaccta 
      tcaaataga atgataata aataactga ataaaaata 
      aatattgtt aaaagcaca aatctttta aaataaaat 
      agatagata tttatagag agtatgcat tgcatggat 
      taaaacata gttatatac cccataaat agagagtaa 
      attttattg tctatttta cttaaacaa atcgatata 
      tgaactgaa agttgacac ctaatagtg aaaacaata 
      agaagaaga cgagggaat cgatacatt ccgttagaa 
      tatatttga aagtttcaa taaaacatc ttcgagata 
      atactgact tgttcgcct gatgtatta cttttttag 
      gcattgcag acatttatt agaacgatg taattttgt 
      tcaaaggca attatagtc atgacaaat agcacaatt 
      ttatttcta ggtacttaa caaatttta tagaactgt 
      agaagcatt taaatggaa cgacattcc ataaaataa 
      aaatattat tctttccgt tacgagttt tattagcca 
      tgtgattgg atccaaaca tttatgcta aaatcgaca 
      gcacttgga aaatttggg ccacctcac aaattacga 
      gaacaaagt attttatgt cctgaaagt agttcaagt 
      gttctccac ttatttaag aaatattcc cttttttat 
      tgttcgtgt cgcttcttg attacagag aataagacg 
      tgtcctgtt attttatgg aaaaataaa aaaattgtg 
      ttttatttt cgacatctc catttttat gtttgcggt 
      ttccctgta ggccccatt ttttattcg ctttgccgt 
      aaatgcaag cataacttt catggcatc gtaacgttt 
      tgagtttcg ccagttttt gtatgtcta caaaataga 
      agatttttt ttctaagcg ccatctatc acaaatttc 
      cgtaaaatg tcgtatcct tttctttat ttcgtcaat 
      tatggtgga agcgcaaca agtgtttta attttcttg 
      ctaaaaata ctttataga attatacaa atttgattt 
      tacgccatg aagactgtg tagtgttaa gtttaccca 
      tttattgag tttccactt taacacctc acgatttaa 
      cctccaaac ctgcccaac cgctgggga acaaaacaa 
      tacaaaact tggccagaa atggacatg aaaaacaat 
      ttggcattg aagaaaaaa cgggaacta cttgggggc 
      atcaacaag cgaaaaatg accctgagg aagtacatg 
      gacaacctc aaacagagt gagtgtcga ctggacatt 
      acccagatc acaaagtgc gtttttttg ggctctctg 
      tgcctcaaa cccgtgccc catcttttc gattcgccc 
      ttgcttctg gaggagctt gatgcgaaa gttggcagt 
      gtggggact gaatttgag tacattgaa atgaaatca 
      gttatgagt gataaggaa gctgttaca aaagactcc 
      gttaaacgg gggcttaag aaaaaaaca ctaaactcg 
      ccgaagatt aaaaagaaa acgctgaaa gtaaagact 
      gacattaga acgactccc aatggctac aacagtgtg 
      aaaagcgaa agtgcaagt gaacttgtt gaagccaca 
      acagccatg agtcccgtg cttccagta ataaacacc 
      gaagaaact tccccagtc ctgtcttca ttccaaacc 
      gaaccgagc gaaagtatg tccacaagt gaggaaaat 
      caagtggaa acacccttc cctgacttt ctagacatt 
      aaactccta aaccagaac atcaaagac attaagggc 
      gtaaacccc gaccccttc gccttcgaa gaagacgag 
      gcagagcgc ctgagacgc gagccaaca cctgaatta 
      attttatcc gactctgat gaagacgca attgaccaa 
      ttaatgtac aacatcatg gacattaaa gtgggacca 
      aacgttttc gacaaagac ttctttgac atgcccccc 
      tctgacatt gaagaggac tacgaagag tactcgaac 
      gattcgcag gaaaataaa aacgagcca aacaccgtc 
      atcaccaga gggaaaaag tacagcaaa cgcgccccc 
      caatgcaag atctgttcg caagtcttc tccacctat 
      atgcagctc aaaaaacac aaactggcc cacgatggc 
      gacaaaccc tacttctgt ctgaagtgc aacgaaaac 
      caccagact ctcgaccag ctcattgcg catctgagg 
      acgcatcag gggaagcgg ccctacgtg tgcaagaag 
      tgcaaccag ggcttcaag acgctcaag ggcttggag 
      agccaccac ccggtgcac gtgttgaag aaagtcagt 
      tcgcagaag cgagtcttc aggtgtgag gtttgtaac 
      aaagagttc cacaagttg tgcgatttg gaaagacat 
      acgagagtg catacgggt gaaaaaccg tcgatttgt 
      aacatttgc aacaagcgt ttccaacaa gcgcacaat 
      ttaaataaa catttgctt attcacacg caggaaaag 
      ccgtatgag tgtgatgtt tgtcacaag aagttcggc 
      cggaacgat gttttaaac agacatttg cttacgcat 
      tcggttaaa aaaccgaac cggtgcgag atttgtatg 
      aagagtttc attcgggtg tcgcagctt ctagctcat 
      aataaaaaa caccataca gaaatggct tcctagttt 
      ttggttggg ggttgttgc cgttttaag gcagcgatg 
      atctctttt tttttgtct ttaggtaat gttgttttg 
      cagaactat gagtgttta cttacggtg cgttttggg 
      atcagaagt ttttgttaa gttaagagt taatttcat 
      ttaattatt tctcacttt accacaaga gaataatga 
      aaataggcg aaaattatc atttacaaa gtttttttt 
      aaatcatta aaaatgtaa atgggtaat tctttgttt 
      agctttcgt aattttgac gttttagtc agatttaaa 
      ttatccaat ttatttatt ttataatct ataagacca 
      caattttta attcggcag gagatctgt tttaatttt 
      ttataacaa tgtgttgag aaaaatact cttttttcg 
      ttatatatt tttttatag atcccaata cttggtcgg 
      tttgcaaat gaatttgca ataatttta ttaaagccg 
      attactata agtactaaa ctaccaaaa atgaggatt 
      ttcaatttc actacttat actgcagtt gtttttttt 
      tttaattat ttagacgtt atttgtact gtactgtac 
      tgagtagtt acctacaac catactttt gtagtttta 
      attgttatt tttttactt gaagaattt tagttaggt 
      ttgtagtgg tttgtttaa gtttcatta ttactttgc 
      aacgttgca tctacttaa aagtgtaaa tatgaacta 
      ttaattttg tcacctgaa aactcttct ctcagtgca 
      atagcttca tttttattc aggtgataa aattgttaa 
      tcccctttt gtacaaaat tattatgca agtgtgata 
      gtattacaa agtgccttt atgtatctt aacaataaa 
      ttgttgtgc cttttatta catttgtaa aatgaaaac 
      gagtgaaaa ttactgcaa aaagaagtc tatttttgt 
      cagtgattt attgcctta cctagtttt tttacagtt 
      agtttaagc atacttata cacttactt gtatgtata 
      aataactta tatactttt agatggata taagatatt 
      aatagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY