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Gene information
General information
TC001563 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2528397..2531659
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001563
    iBeetle screen iB_00261
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001563 RA
      MHNVHKLIV LCLVVGAYS DEHNHIYGD NEEVILWMN 
      TVGPYHNRQ ETYAYFSLP FCVGSKDTI NHYHETLSE 
      ALQGVELEF SGIDIEFKS NVQKGEYCA VQLNEDKYK 
      VFVYAVKNH YWYQMYIDD LPIWGVVGE IKDNNYYIW 
      THKKFEIGY NGKQIIDVN LTSEDKVLL TPNVKLSFT 
      YEVIWKPTE TKFEDRFDK YLDYNFFQH RIHWFSIFN 
      SFMMVIFLV GLVSMILMR TLRKDYARY SKDEDIDDM 
      ERDLGDEYG WKQVHGDVF RPASNSLVF SSLIGAGHQ 
      VTTVVFCVI VFAILGELY TERGSLLST AIFVYAITS 
      PINGYFGGS LYARMGGKL WIRQMTVSA FMLPAFVCG 
      TAFFINFIA IYYHASRAI PFGTMVAVT CICLFIILP 
      LTLVGTVLG RNLAGQPDY PCRINAVPR PIPEKKWFM 
      EPGVIILMG GILPFASIF IEMYFIFTS FWAYKIYYV 
      YGFMLLVFI ILMIVTVCV TIVCTYFLL NAEDYRWQW 
      TSFLAAAST SAYVYIYAI YYFFFKTKM YGLFQTAFY 
      FGYMALFSG ALGIMCGTV GYVGTSVFV RKIYSTVKI 
      D
    CDS sequence
    • >TC001563 RA
      atgcataac gtacataaa cttatcgtt ctgtgtctc 
      gttgttggg gcgtattca gatgaacat aatcacata 
      tatggagac aatgaagag gtgatactt tggatgaac 
      actgttggt ccttatcac aaccgacaa gaaacttac 
      gcttatttt tccctaccg ttttgcgtg ggctcaaag 
      gacacaatc aaccactac catgaaacg ctcagcgag 
      gccctgcaa ggagttgag ttggaattc agtgggatt 
      gacatcgag ttcaaaagc aatgttcaa aagggtgaa 
      tactgcgcc gtgcagtta aatgaggac aaatacaaa 
      gtgtttgtc tacgcggtc aaaaaccac tactggtac 
      caaatgtac attgatgat ttgcccatt tggggcgtc 
      gtgggtgaa atcaaagac aacaactat tacatttgg 
      acccacaaa aagttcgag attggctac aatggcaag 
      caaattatt gacgttaat ctcacatca gaggataaa 
      gtgcttttg actcctaac gtgaaacta tccttcacc 
      tatgaggtc atttggaag ccgactgag accaaattt 
      gaggataga ttcgataaa tatttggac tataatttc 
      tttcaacac agaattcat tggtttagc attttcaat 
      agtttcatg atggtcata tttttggtc ggtttggtg 
      tcgatgatt ttgatgcgc acgttgcgg aaagattat 
      gcaagatac agtaaagat gaagatatt gatgacatg 
      gagagagat ttgggtgat gaatacggc tggaagcaa 
      gtccatggc gacgtattc cgtccagct tccaactcg 
      ctcgttttc tcgtcgctt attggagcc ggccaccaa 
      gtaacaaca gtcgtattc tgtgttata gttttcgca 
      atccttggc gaattatac accgaacga ggatctctc 
      ctatccacc gcgatattc gtctatgca ataacctcc 
      ccaataaac ggctacttc gggggttcc ctttacgcg 
      cgaatgggg ggcaagctc tggatcagg caaatgaca 
      gtgtctgca ttcatgctg ccagccttt gtttgtggc 
      accgcgttc tttatcaat ttcatcgcg atttattat 
      cacgcatca agggcgatt ccttttggg acaatggtc 
      gcagtcact tgtatttgt ttgtttatt attttgccc 
      ctaactttg gtgggtact gtcttgggg cgcaattta 
      gcaggccaa cctgattat ccctgccgt atcaacgca 
      gttccgcgg ccaattccg gaaaagaag tggtttatg 
      gagccagga gtgattatt ttgatgggc gggattttg 
      ccatttgct agcatattt atcgaaatg tactttatt 
      tttacgtcg ttctgggct tacaaaatt tattacgtg 
      tatggattt atgctgctg gtgtttatt atactgatg 
      attgtaact gtatgtgtg acaatagtt tgtacgtac 
      tttctactc aatgctgag gattatcgc tggcaatgg 
      acgtctttt ctggcagcg gcttccacc tctgcctat 
      gtctacatc tatgcgatt tattacttc ttttttaaa 
      acaaagatg tatggactg tttcagact gcgttttat 
      ttcggatac atggcgctc ttcagtgga gcactcggt 
      ataatgtgt ggtacggtg ggttacgta ggtaccagt 
      gtgtttgtt aggaagata tattccact gttaaaatt 
      gactga
    mRNA sequence
    • >TC001563 RA
      gaactgtca attcaacgt caacatttt ctgatctac 
      gtttgtgta gttatgggt ttttaggag attccggta 
      attcatgga cttgaaacg tgccgtttt cccgtaaaa 
      acactgact taataatta gtgaaccac aattagagt 
      gaataatgc ataacgtac ataaactta tcgttctgt 
      gtctcgttg ttggggcgt attcagatg aacataatc 
      acatatatg gagacaatg aagaggtga tactttgga 
      tgaacactg ttggtcctt atcacaacc gacaagaaa 
      cttacgctt atttttccc taccgtttt gcgtgggct 
      caaaggaca caatcaacc actaccatg aaacgctca 
      gcgaggccc tgcaaggag ttgagttgg aattcagtg 
      ggattgaca tcgagttca aaagcaatg ttcaaaagg 
      gtgaatact gcgccgtgc agttaaatg aggacaaat 
      acaaagtgt ttgtctacg cggtcaaaa accactact 
      ggtaccaaa tgtacattg atgatttgc ccatttggg 
      gcgtcgtgg gtgaaatca aagacaaca actattaca 
      tttggaccc acaaaaagt tcgagattg gctacaatg 
      gcaagcaaa ttattgacg ttaatctca catcagagg 
      ataaagtgc ttttgactc ctaacgtga aactatcct 
      tcacctatg aggtcattt ggaagccga ctgagacca 
      aatttgagg atagattcg ataaatatt tggactata 
      atttctttc aacacagaa ttcattggt ttagcattt 
      tcaatagtt tcatgatgg tcatatttt tggtcggtt 
      tggtgtcga tgattttga tgcgcacgt tgcggaaag 
      attatgcaa gatacagta aagatgaag atattgatg 
      acatggaga gagatttgg gtgatgaat acggctgga 
      agcaagtcc atggcgacg tattccgtc cagcttcca 
      actcgctcg ttttctcgt cgcttattg gagccggcc 
      accaagtaa caacagtcg tattctgtg ttatagttt 
      tcgcaatcc ttggcgaat tatacaccg aacgaggat 
      ctctcctat ccaccgcga tattcgtct atgcaataa 
      cctccccaa taaacggct acttcgggg gttcccttt 
      acgcgcgaa tggggggca agctctgga tcaggcaaa 
      tgacagtgt ctgcattca tgctgccag cctttgttt 
      gtggcaccg cgttcttta tcaatttca tcgcgattt 
      attatcacg catcaaggg cgattcctt ttgggacaa 
      tggtcgcag tcacttgta tttgtttgt ttattattt 
      tgcccctaa ctttggtgg gtactgtct tggggcgca 
      atttagcag gccaacctg attatccct gccgtatca 
      acgcagttc cgcggccaa ttccggaaa agaagtggt 
      ttatggagc caggagtga ttattttga tgggcggga 
      ttttgccat ttgctagca tatttatcg aaatgtact 
      ttattttta cgtcgttct gggcttaca aaatttatt 
      acgtgtatg gatttatgc tgctggtgt ttattatac 
      tgatgattg taactgtat gtgtgacaa tagtttgta 
      cgtactttc tactcaatg ctgaggatt atcgctggc 
      aatggacgt cttttctgg cagcggctt ccacctctg 
      cctatgtct acatctatg cgatttatt acttctttt 
      ttaaaacaa agatgtatg gactgtttc agactgcgt 
      tttatttcg gatacatgg cgctcttca gtggagcac 
      tcggtataa tgtgtggta cggtgggtt acgtaggta 
      ccagtgtgt ttgttagga agatatatt ccactgtta 
      aaattgact gaagtgaat gagccattt gtaaatagt 
      tggaaatcc gtgcggact ttatacatc ttacatgtt 
      aactggatg tgtgaagaa ttcgttttt caaatttca 
      tttgcctta ttgtgattc gaaataaac tatttatta 
      taaaattat tacgttttt agattacga ctcctacat 
      acgttcgca ccttggaag tacttaact tttagttca 
      agttcttgt cataaaatc agtggttat gttatttta 
      tagtgtgat aatttaaaa attagccca gttggccaa 
      attatttaa agtgttctg tacattttt ggccaacag 
      ggctgaaat gttacttca actgtacaa aattttcgt 
      attgaatac gaagttcaa atttttgtt ttgtaaaat 
      tgtaatgtt ttttataaa taaataact agaatgaac 
      gatgacttg tgttgtggt cactaacaa actttctac 
      tgtaaaaat aggtaataa attactgga aacatt