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Gene information
General information
TC001555 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3426177..3442019
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001555
    iBeetle screen iB_00259

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    lateral oviduct number degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001555 RA
      MSVIIRLQN LPWSANALD IRQFFHGLS IPEGGVHIV 
GGEQGDAFI AFSTDEDAR QAFARNNGK IKEIQISLM 
LSSRTEMQK VIEAARAQS YAAFMQNPT PMPAAVPAI 
IPSAVPELK KENSKDRDT KDKRDRKRS RSRSRDRKD 
RDRRRYRDR SRSRSRERR ERRRRERSR SRGRSRSRD 
RKNRDRKRS ADNFNQKDP KKMPEVWAN PNSNDSIPA 
APLPPSLFG NFPGNLDDA RRNLNALTG QVGNNTNGR 
SNNFPGCNN FGMNRVRDS WPPQNVPND SNDEGFLNR 
SDDGLDARQ RPGPNFQNR RFPRNNNFP HPEDEQQFQ 
AEREPLADC CVRLQPFYG GYGDIRRFF QGSFISNVG 
IKFINDDFG HRTGIVYIQ FGHPLGKNE ALRKNGEVL 
NGVKVEVAH IDDEEFDEA VDRYQPENF PQSVDTNKF 
RSKNITKYF NKPEPELEV FSCLTVDDL PTYVKEQDI 
LKLFSPRPL LALFLTPKP RGGHIAYVK FGSVEEAKK 
ALEENINHK VEGKPVTIK PCDDREFEN ISQQHNVDL 
ECKPSPLPQ LDTDCINLT RLPLQTNNI EIADFFSDI 
GVRPTKIHL MSNRMGFTG QAFCEFKTK EDAAQALKK 
DNSSFGPNI VSVQPFPRE EMEHILQQS PAPNAPLLQ 
PPMMPRAPT MNQNRPFFP RNSYEGGPP RGGHRFMGH 
RAPRPRFMN AVPADDAPL GCTVYMDNV PYKAGTNEI 
LDFFDGYDI TNNVSRRFN PNNTPSAEA KVIFSSPEE 
AYRAVQEKN GQKIWERPI YLKQV
    CDS sequence
    • >TC001555 RA
      atgagtgtc attatacgc ctacaaaac ttaccgtgg 
tctgctaat gctctcgac atacgtcaa ttctttcac 
ggattaagc attcctgag ggaggagtt cacatcgtt 
ggtggagaa caaggcgac gcgttcatc gctttcagt 
actgacgaa gacgctcgc caggccttt gcacgcaac 
aatggcaaa atcaaagaa atccaaatc agcctcatg 
ctcagctcc cgaaccgaa atgcaaaaa gtcatcgaa 
gcggcacga gcccaatcc tacgccgca ttcatgcaa 
aaccccact ccgatgccg gccgccgtc cccgccata 
atcccctca gcagtcccc gaactcaaa aaagagaac 
agcaaagac cgcgacacc aaagacaaa cgcgaccgc 
aaacgctcc cgctcgcgg tcccgcgac cgcaaagac 
cgcgaccgc cggcggtac agggacagg agcaggtcg 
cggtccaga gagcggcgc gaacgccgc cgcagagag 
cggagccgc agtcggggc cgctcccgc tccagagac 
cgcaaaaac agggacagg aaacgctcg gcggacaat 
ttcaaccag aaagacccg aagaaaatg ccagaggtg 
tgggccaac cccaactcc aacgacagt attccggca 
gcgccgctc ccgccgagt ttattcggg aactttccg 
gggaatctg gacgacgcg cgacggaac ttgaacgca 
ctcacgggg caagttggc aataacacc aacgggagg 
agtaataat tttccgggg tgtaacaat ttcgggatg 
aacagggtg agggatagt tggccgcca cagaacgtg 
ccgaatgat agcaatgac gaagggttt cttaaccga 
tcggatgac gggcttgat gcgagacag cgtccaggc 
ccaaacttc caaaaccgt cgatttccc cgcaataac 
aatttccca caccctgaa gacgagcag caattccaa 
gcggaacgc gaacctttg gccgactgt tgcgttcga 
ttgcagccc ttttatggg ggctacggt gacattaga 
cgcttcttc caaggctct ttcatcagt aacgtggga 
atcaaattc atcaatgac gatttcggc caccgaact 
ggcattgtc tacatccag ttcggtcac ccactgggc 
aaaaacgaa gcgttgcgc aaaaacggc gaagtgtta 
aatggggtc aaagtcgag gtggcgcac atcgacgac 
gaggaattc gacgaggct gtggaccgc taccaaccg 
gagaacttc ccccagagc gtggacacc aacaaattc 
cggagcaaa aacattacc aaatacttc aacaaaccg 
gagccggag ttggaagtg ttctcgtgc ttgacggtt 
gacgatcta ccaacctat gtcaaagaa caggacatc 
ctcaagcta ttttcccca cggccgctc cttgcactg 
ttcctcaca ccgaaacca cgtggcggt cacatcgcc 
tacgtcaag ttcgggtcg gtcgaagaa gccaaaaag 
gcgctagag gagaacatc aaccacaaa gttgaaggc 
aaacctgtc acgatcaaa ccgtgcgat gaccgcgag 
ttcgagaac ataagccag caacacaac gtcgatttg 
gagtgcaaa cccagcccc ctaccccaa ctagacacc 
gactgtatc aacctaacc cgtctgccg ctccaaacc 
aacaacatc gaaatcgcc gacttcttc tcggacatt 
ggggtccga ccgacaaaa atccacctc atgagcaac 
cgcatgggg ttcacaggc caggccttc tgcgagttt 
aagaccaaa gaagacgcg gctcaagcc ctcaagaaa 
gataattca agttttggc ccaaatatc gtatcagtt 
cagccgttt ccgcgcgag gagatggag cacatcctc 
cagcagtct cccgccccc aacgccccg cttttgcag 
ccgcccatg atgccccgg gcccccacc atgaaccag 
aaccggccg ttcttcccc aggaacagc tacgagggg 
ggcccgccg cgagggggc caccggttc atggggcac 
agggcgccc aggcccagg tttatgaac gcggtgccg 
gcggatgat gcccccttg gggtgcacg gtgtacatg 
gataatgtg ccgtacaaa gcggggacg aacgagatt 
ttggatttc tttgacggt tatgatatt acgaataat 
gtcagtcgg aggtttaat ccgaataat acgccgagt 
gccgaggct aaggtgatt tttagttcg ccggaggag 
gcgtatagg gcggttcaa gagaagaac gggcagaag 
atttgggag aggcccatc tatttgaag caagtgtag
    mRNA sequence
    • >TC001555 RA
      ctcgctgac ataagcgtc acttcgtgg tttttgtcg 
atttttcga tcaaagttt ctcaagatc catcatttt 
atcgtaaaa atattacta ttagagcgg gttgcggtg 
aagaatttt ttttagttc gagttctag gttaagatg 
agtgtcatt atacgccta caaaactta ccgtggtct 
gctaatgct ctcgacata cgtcaattc tttcacgga 
ttaagcatt cctgaggga ggagttcac atcgttggt 
ggagaacaa ggcgacgcg ttcatcgct ttcagtact 
gacgaagac gctcgccag gcctttgca cgcaacaat 
ggcaaaatc aaagaaatc caaatcagc ctcatgctc 
agctcccga accgaaatg caaaaagtc atcgaagcg 
gcacgagcc caatcctac gccgcattc atgcaaaac 
cccactccg atgccggcc gccgtcccc gccataatc 
ccctcagca gtccccgaa ctcaaaaaa gagaacagc 
aaagaccgc gacaccaaa gacaaacgc gaccgcaaa 
cgctcccgc tcgcggtcc cgcgaccgc aaagaccgc 
gaccgccgg cggtacagg gacaggagc aggtcgcgg 
tccagagag cggcgcgaa cgccgccgc agagagcgg 
agccgcagt cggggccgc tcccgctcc agagaccgc 
aaaaacagg gacaggaaa cgctcggcg gacaatttc 
aaccagaaa gacccgaag aaaatgcca gaggtgtgg 
gccaacccc aactccaac gacagtatt ccggcagcg 
ccgctcccg ccgagttta ttcgggaac tttccgggg 
aatctggac gacgcgcga cggaacttg aacgcactc 
acggggcaa gttggcaat aacaccaac gggaggagt 
aataatttt ccggggtgt aacaatttc gggatgaac 
agggtgagg gatagttgg ccgccacag aacgtgccg 
aatgatagc aatgacgaa gggtttctt aaccgatcg 
gatgacggg cttgatgcg agacagcgt ccaggccca 
aacttccaa aaccgtcga tttccccgc aataacaat 
ttcccacac cctgaagac gagcagcaa ttccaagcg 
gaacgcgaa cctttggcc gactgttgc gttcgattg 
cagcccttt tatgggggc tacggtgac attagacgc 
ttcttccaa ggctctttc atcagtaac gtgggaatc 
aaattcatc aatgacgat ttcggccac cgaactggc 
attgtctac atccagttc ggtcaccca ctgggcaaa 
aacgaagcg ttgcgcaaa aacggcgaa gtgttaaat 
ggggtcaaa gtcgaggtg gcgcacatc gacgacgag 
gaattcgac gaggctgtg gaccgctac caaccggag 
aacttcccc cagagcgtg gacaccaac aaattccgg 
agcaaaaac attaccaaa tacttcaac aaaccggag 
ccggagttg gaagtgttc tcgtgcttg acggttgac 
gatctacca acctatgtc aaagaacag gacatcctc 
aagctattt tccccacgg ccgctcctt gcactgttc 
ctcacaccg aaaccacgt ggcggtcac atcgcctac 
gtcaagttc gggtcggtc gaagaagcc aaaaaggcg 
ctagaggag aacatcaac cacaaagtt gaaggcaaa 
cctgtcacg atcaaaccg tgcgatgac cgcgagttc 
gagaacata agccagcaa cacaacgtc gatttggag 
tgcaaaccc agcccccta ccccaacta gacaccgac 
tgtatcaac ctaacccgt ctgccgctc caaaccaac 
aacatcgaa atcgccgac ttcttctcg gacattggg 
gtccgaccg acaaaaatc cacctcatg agcaaccgc 
atggggttc acaggccag gccttctgc gagtttaag 
accaaagaa gacgcggct caagccctc aagaaagat 
aattcaagt tttggccca aatatcgta tcagttcag 
ccgtttccg cgcgaggag atggagcac atcctccag 
cagtctccc gcccccaac gccccgctt ttgcagccg 
cccatgatg ccccgggcc cccaccatg aaccagaac 
cggccgttc ttccccagg aacagctac gaggggggc 
ccgccgcga gggggccac cggttcatg gggcacagg 
gcgcccagg cccaggttt atgaacgcg gtgccggcg 
gatgatgcc cccttgggg tgcacggtg tacatggat 
aatgtgccg tacaaagcg gggacgaac gagattttg 
gatttcttt gacggttat gatattacg aataatgtc 
agtcggagg tttaatccg aataatacg ccgagtgcc 
gaggctaag gtgattttt agttcgccg gaggaggcg 
tatagggcg gttcaagag aagaacggg cagaagatt 
tgggagagg cccatctat ttgaagcaa gtgtagact 
gaacgatat tttagattg tagttgtta taagttttt 
ttattgtat gtatgaatg atcacgttc ggttaggaa 
tgtggtttc ttgagttgt aaattattg cgttttgaa 
gagttcttt attgaatat ttttagagc aagtgtata 
gaaaactgg ataaatgtg catattcgc gtgtatgta 
tttatgaac taatcatat gaaatgatt catctcata 
attttctat ttctataat taagttact atgtaaaga 
gtatcctaa taaaatatg ccgtgcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY