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Gene information
General information
TC001555 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2347666..2363508
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001555
    iBeetle screen iB_00259
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    lateral oviduct number degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001555 RA
      MSVIIRLQN LPWSANALD IRQFFHGLS IPEGGVHIV 
      GGEQGDAFI AFSTDEDAR QAFARNNGK IKEIQISLM 
      LSSRTEMQK VIEAARAQS YAAFMQNPT PMPAAVPAI 
      IPSAVPELK KENSKDRDT KDKRDRKRS RSRSRDRKD 
      RDRRRYRDR SRSRSRERR ERRRRERSR SRGRSRSRD 
      RKNRDRKRS ADNFNQKDP KKMPEVWAN PNSNDSIPA 
      APLPPSLFG NFPGNLDDA RRNLNALTG QVGNNTNGR 
      SNNFPGCNN FGMNRVRDS WPPQNVPND SNDEGFLNR 
      SDDGLDARQ RPGPNFQNR RFPRNNNFP HPEDEQQFQ 
      AEREPLADC CVRLQPFYG GYGDIRRFF QGSFISNVG 
      IKFINDDFG HRTGIVYIQ FGHPLGKNE ALRKNGEVL 
      NGVKVEVAH IDDEEFDEA VDRYQPENF PQSVDTNKF 
      RSKNITKYF NKPEPELEV FSCLTVDDL PTYVKEQDI 
      LKLFSPRPL LALFLTPKP RGGHIAYVK FGSVEEAKK 
      ALEENINHK VEGKPVTIK PCDDREFEN ISQQHNVDL 
      ECKPSPLPQ LDTDCINLT RLPLQTNNI EIADFFSDI 
      GVRPTKIHL MSNRMGFTG QAFCEFKTK EDAAQALKK 
      DNSSFGPNI VSVQPFPRE EMEHILQQS PAPNAPLLQ 
      PPMMPRAPT MNQNRPFFP RNSYEGGPP RGGHRFMGH 
      RAPRPRFMN AVPADDAPL GCTVYMDNV PYKAGTNEI 
      LDFFDGYDI TNNVSRRFN PNNTPSAEA KVIFSSPEE 
      AYRAVQEKN GQKIWERPI YLKQV
    CDS sequence
    • >TC001555 RA
      atgagtgtc attatacgc ctacaaaac ttaccgtgg 
      tctgctaat gctctcgac atacgtcaa ttctttcac 
      ggattaagc attcctgag ggaggagtt cacatcgtt 
      ggtggagaa caaggcgac gcgttcatc gctttcagt 
      actgacgaa gacgctcgc caggccttt gcacgcaac 
      aatggcaaa atcaaagaa atccaaatc agcctcatg 
      ctcagctcc cgaaccgaa atgcaaaaa gtcatcgaa 
      gcggcacga gcccaatcc tacgccgca ttcatgcaa 
      aaccccact ccgatgccg gccgccgtc cccgccata 
      atcccctca gcagtcccc gaactcaaa aaagagaac 
      agcaaagac cgcgacacc aaagacaaa cgcgaccgc 
      aaacgctcc cgctcgcgg tcccgcgac cgcaaagac 
      cgcgaccgc cggcggtac agggacagg agcaggtcg 
      cggtccaga gagcggcgc gaacgccgc cgcagagag 
      cggagccgc agtcggggc cgctcccgc tccagagac 
      cgcaaaaac agggacagg aaacgctcg gcggacaat 
      ttcaaccag aaagacccg aagaaaatg ccagaggtg 
      tgggccaac cccaactcc aacgacagt attccggca 
      gcgccgctc ccgccgagt ttattcggg aactttccg 
      gggaatctg gacgacgcg cgacggaac ttgaacgca 
      ctcacgggg caagttggc aataacacc aacgggagg 
      agtaataat tttccgggg tgtaacaat ttcgggatg 
      aacagggtg agggatagt tggccgcca cagaacgtg 
      ccgaatgat agcaatgac gaagggttt cttaaccga 
      tcggatgac gggcttgat gcgagacag cgtccaggc 
      ccaaacttc caaaaccgt cgatttccc cgcaataac 
      aatttccca caccctgaa gacgagcag caattccaa 
      gcggaacgc gaacctttg gccgactgt tgcgttcga 
      ttgcagccc ttttatggg ggctacggt gacattaga 
      cgcttcttc caaggctct ttcatcagt aacgtggga 
      atcaaattc atcaatgac gatttcggc caccgaact 
      ggcattgtc tacatccag ttcggtcac ccactgggc 
      aaaaacgaa gcgttgcgc aaaaacggc gaagtgtta 
      aatggggtc aaagtcgag gtggcgcac atcgacgac 
      gaggaattc gacgaggct gtggaccgc taccaaccg 
      gagaacttc ccccagagc gtggacacc aacaaattc 
      cggagcaaa aacattacc aaatacttc aacaaaccg 
      gagccggag ttggaagtg ttctcgtgc ttgacggtt 
      gacgatcta ccaacctat gtcaaagaa caggacatc 
      ctcaagcta ttttcccca cggccgctc cttgcactg 
      ttcctcaca ccgaaacca cgtggcggt cacatcgcc 
      tacgtcaag ttcgggtcg gtcgaagaa gccaaaaag 
      gcgctagag gagaacatc aaccacaaa gttgaaggc 
      aaacctgtc acgatcaaa ccgtgcgat gaccgcgag 
      ttcgagaac ataagccag caacacaac gtcgatttg 
      gagtgcaaa cccagcccc ctaccccaa ctagacacc 
      gactgtatc aacctaacc cgtctgccg ctccaaacc 
      aacaacatc gaaatcgcc gacttcttc tcggacatt 
      ggggtccga ccgacaaaa atccacctc atgagcaac 
      cgcatgggg ttcacaggc caggccttc tgcgagttt 
      aagaccaaa gaagacgcg gctcaagcc ctcaagaaa 
      gataattca agttttggc ccaaatatc gtatcagtt 
      cagccgttt ccgcgcgag gagatggag cacatcctc 
      cagcagtct cccgccccc aacgccccg cttttgcag 
      ccgcccatg atgccccgg gcccccacc atgaaccag 
      aaccggccg ttcttcccc aggaacagc tacgagggg 
      ggcccgccg cgagggggc caccggttc atggggcac 
      agggcgccc aggcccagg tttatgaac gcggtgccg 
      gcggatgat gcccccttg gggtgcacg gtgtacatg 
      gataatgtg ccgtacaaa gcggggacg aacgagatt 
      ttggatttc tttgacggt tatgatatt acgaataat 
      gtcagtcgg aggtttaat ccgaataat acgccgagt 
      gccgaggct aaggtgatt tttagttcg ccggaggag 
      gcgtatagg gcggttcaa gagaagaac gggcagaag 
      atttgggag aggcccatc tatttgaag caagtgtag
    mRNA sequence
    • >TC001555 RA
      ctcgctgac ataagcgtc acttcgtgg tttttgtcg 
      atttttcga tcaaagttt ctcaagatc catcatttt 
      atcgtaaaa atattacta ttagagcgg gttgcggtg 
      aagaatttt ttttagttc gagttctag gttaagatg 
      agtgtcatt atacgccta caaaactta ccgtggtct 
      gctaatgct ctcgacata cgtcaattc tttcacgga 
      ttaagcatt cctgaggga ggagttcac atcgttggt 
      ggagaacaa ggcgacgcg ttcatcgct ttcagtact 
      gacgaagac gctcgccag gcctttgca cgcaacaat 
      ggcaaaatc aaagaaatc caaatcagc ctcatgctc 
      agctcccga accgaaatg caaaaagtc atcgaagcg 
      gcacgagcc caatcctac gccgcattc atgcaaaac 
      cccactccg atgccggcc gccgtcccc gccataatc 
      ccctcagca gtccccgaa ctcaaaaaa gagaacagc 
      aaagaccgc gacaccaaa gacaaacgc gaccgcaaa 
      cgctcccgc tcgcggtcc cgcgaccgc aaagaccgc 
      gaccgccgg cggtacagg gacaggagc aggtcgcgg 
      tccagagag cggcgcgaa cgccgccgc agagagcgg 
      agccgcagt cggggccgc tcccgctcc agagaccgc 
      aaaaacagg gacaggaaa cgctcggcg gacaatttc 
      aaccagaaa gacccgaag aaaatgcca gaggtgtgg 
      gccaacccc aactccaac gacagtatt ccggcagcg 
      ccgctcccg ccgagttta ttcgggaac tttccgggg 
      aatctggac gacgcgcga cggaacttg aacgcactc 
      acggggcaa gttggcaat aacaccaac gggaggagt 
      aataatttt ccggggtgt aacaatttc gggatgaac 
      agggtgagg gatagttgg ccgccacag aacgtgccg 
      aatgatagc aatgacgaa gggtttctt aaccgatcg 
      gatgacggg cttgatgcg agacagcgt ccaggccca 
      aacttccaa aaccgtcga tttccccgc aataacaat 
      ttcccacac cctgaagac gagcagcaa ttccaagcg 
      gaacgcgaa cctttggcc gactgttgc gttcgattg 
      cagcccttt tatgggggc tacggtgac attagacgc 
      ttcttccaa ggctctttc atcagtaac gtgggaatc 
      aaattcatc aatgacgat ttcggccac cgaactggc 
      attgtctac atccagttc ggtcaccca ctgggcaaa 
      aacgaagcg ttgcgcaaa aacggcgaa gtgttaaat 
      ggggtcaaa gtcgaggtg gcgcacatc gacgacgag 
      gaattcgac gaggctgtg gaccgctac caaccggag 
      aacttcccc cagagcgtg gacaccaac aaattccgg 
      agcaaaaac attaccaaa tacttcaac aaaccggag 
      ccggagttg gaagtgttc tcgtgcttg acggttgac 
      gatctacca acctatgtc aaagaacag gacatcctc 
      aagctattt tccccacgg ccgctcctt gcactgttc 
      ctcacaccg aaaccacgt ggcggtcac atcgcctac 
      gtcaagttc gggtcggtc gaagaagcc aaaaaggcg 
      ctagaggag aacatcaac cacaaagtt gaaggcaaa 
      cctgtcacg atcaaaccg tgcgatgac cgcgagttc 
      gagaacata agccagcaa cacaacgtc gatttggag 
      tgcaaaccc agcccccta ccccaacta gacaccgac 
      tgtatcaac ctaacccgt ctgccgctc caaaccaac 
      aacatcgaa atcgccgac ttcttctcg gacattggg 
      gtccgaccg acaaaaatc cacctcatg agcaaccgc 
      atggggttc acaggccag gccttctgc gagtttaag 
      accaaagaa gacgcggct caagccctc aagaaagat 
      aattcaagt tttggccca aatatcgta tcagttcag 
      ccgtttccg cgcgaggag atggagcac atcctccag 
      cagtctccc gcccccaac gccccgctt ttgcagccg 
      cccatgatg ccccgggcc cccaccatg aaccagaac 
      cggccgttc ttccccagg aacagctac gaggggggc 
      ccgccgcga gggggccac cggttcatg gggcacagg 
      gcgcccagg cccaggttt atgaacgcg gtgccggcg 
      gatgatgcc cccttgggg tgcacggtg tacatggat 
      aatgtgccg tacaaagcg gggacgaac gagattttg 
      gatttcttt gacggttat gatattacg aataatgtc 
      agtcggagg tttaatccg aataatacg ccgagtgcc 
      gaggctaag gtgattttt agttcgccg gaggaggcg 
      tatagggcg gttcaagag aagaacggg cagaagatt 
      tgggagagg cccatctat ttgaagcaa gtgtagact 
      gaacgatat tttagattg tagttgtta taagttttt 
      ttattgtat gtatgaatg atcacgttc ggttaggaa 
      tgtggtttc ttgagttgt aaattattg cgttttgaa 
      gagttcttt attgaatat ttttagagc aagtgtata 
      gaaaactgg ataaatgtg catattcgc gtgtatgta 
      tttatgaac taatcatat gaaatgatt catctcata 
      attttctat ttctataat taagttact atgtaaaga 
      gtatcctaa taaaatatg ccgtgcata