Status
Please wait ...
Gene information
General information
TC001541 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2518648..2522399
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001541
    iBeetle screen iB_00258

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: ovary phenotype not scorable,
    Animals lost.
    Adult morphology
    low validity due to technical reasons - should be repeated: ovary phenotype not scorable,
    Animals lost.
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection



    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection



    Pupal morphology
    wings partially blistered - Number of animals showing the phenotype: 4

    wings orientation irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001541 RA
      MARPLAPSE RKIAEKLII LNDRGIGML TRIYNIKKA 
      CGDAKSKPG FLSDKNLES SIKSIVRRF PNVDSKNLT 
      AIQGLRNEI IKSLSLYYY TFVDLLDFK DHVCELLTS 
      MGAFQIELD ISLNFELTK HYLDLVTTY VSLMVLLSR 
      VEDRKAVLG LFNAAYEIV HSSPDSSFP RLGNMIMEY 
      EIPFKKLCE EFVPHTKLL LSALNSLLP IYIARNVSA 
      DKWRSELKL SLVGNPASL LKPVIADRM SCEYVSLDT 
      MERWIVFGL LLIHPHLQQ DHLNKLWIN ALESSWVLP 
      LFRDEVIYT HQYICGYFD TIKGYSKRA SEVKDCHSQ 
      AIQKAGYKH RERRKFLRT ALKELGLIL MDQPGLLGP 
      KALMVFMGL CYARDEVLW LLRHNDNPP QQKNKGKST 
      EDLVDRHLP ELLFHMEDL RVLVRKYSQ VMQRYYVQY 
      LSHFDALVL KDMIQKNLG SVGEDECII LSSLCNTIS 
      GLSVKQVED NETFNFFAF RLDWFRLQA YMSTGNALL 
      RIMDNKDLA QFLDTVQFH TKMVDNLDE MLVETSDLS 
      IFCFYNRIF EDQFHMCLE FPAQNRFIV AFPSICSHF 
      QHCTHELCP EERHHIRER SLSVVNVFL DEMAKEAKN 
      IITAICDAQ CKMSDKLLP KNCAHLISQ QMNRKKKEK 
      NKKNTLEIE KPGKESYRK TRENLTTMD KLHMALTEL 
      CYAINYFSN INVWEYTFA PREYLHQHL ENRFARALV 
      GMVMYSPDT NEIAKPSEL LVSVRAYMN VLQTVENYV 
      HIDITRVFN NCLLQQTQP VDSHGEKTI AAIYTQWYS 
      EVLLRRVSA GNIIFSMNQ RSFVCLTAE AAIPFNPEE 
      YSDVNELRA LAELIGPYG MKQLSETLM WHIASQVIE 
      LKKLAEANK DVLLLLRTN FDKPEVMKE QFKKLTSVE 
      NVLQRMTIV GVILSFRQL AQSCLTDVL EQRIPFLLS 
      SILDFRHHL PSGDPLKVV SEMCSAAGI PCKVDPTLV 
      NALKLQKPE VDSDEHLIT CLLMVFVAV SIPKLAKQE 
      QSFYRASLE GHTNNIHCM ALAVNHIFG ALFTICNQG 
      DMEDRMKEF LALASSSLL RLGQEADKE ATKNRESVY 
      LLLDQIVQE SPFLTMDLL ESCFPYALI RNAYHAVYK 
      QEQLLA
    CDS sequence
    • >TC001541 RA
      atggcccgt cctctcgcc cccagcgag cggaaaatc 
      gccgaaaaa ctcatcatc ctcaacgac aggggcatc 
      ggcatgctc acacgcatc tacaacatt aaaaaagcc 
      tgcggagac gccaaatcc aagccgggg tttctctcc 
      gacaagaac ttggagtct tcgatcaag agcattgtg 
      cggcggttt ccgaacgtc gacagcaag aacttgact 
      gcgattcaa gggctcagg aacgagata atcaaatct 
      ttgtcgctg tattactac acgttcgtc gatttgctt 
      gatttcaaa gaccacgtt tgcgagttg ttgactagc 
      atgggggcg tttcaaatc gagcttgat atttcgctc 
      aatttcgaa ctcactaag cattatctt gacttggtg 
      acgacctac gtgtctctg atggtgttg ctgtcgcgg 
      gttgaggac cgcaaggcc gttctgggg ctttttaac 
      gccgcttat gaaatcgtg cattcctcg cccgattca 
      agcttcccg cggttgggc aacatgatc atggagtat 
      gagattccg tttaaaaag ttatgcgag gagtttgtg 
      ccccacact aagctgctt ctaagcgcc ttgaactcg 
      cttctaccc atttatatc gcccggaat gtctcggct 
      gataaatgg cgctctgaa ttgaagttg agtctagta 
      ggcaaccca gcttcgctc ctcaagccg gtgatagcc 
      gaccggatg tcgtgcgag tacgtctcg cttgatacc 
      atggaacgc tggattgtc ttcggtttg ttgctaatt 
      cacccgcac ttgcagcaa gatcatttg aataaatta 
      tggatcaat gcgcttgag tcgtcttgg gtcttgccc 
      ctgttccgg gacgaggtt atctacacc catcagtac 
      atctgtggg tactttgat accatcaag ggctatagt 
      aaaagggcg tccgaggtt aaggattgc cacagtcaa 
      gcgatacaa aaggctggg tacaaacac cgcgaacgc 
      cgaaaattc ctaaggacg gccctcaaa gaattaggc 
      ttgattttg atggaccag ccggggctt ttggggccg 
      aaggccctc atggtcttc atgggtttg tgctacgca 
      cgggacgag gtcttatgg ttgttgcgt cacaacgac 
      aacccaccc caacaaaaa aacaagggt aaatcgacc 
      gaggattta gtcgataga cacctgcca gaactcctc 
      ttccacatg gaagactta cgggtgttg gtccgaaag 
      tacagtcaa gtcatgcaa aggtactac gtccagtac 
      ctctcacac ttcgacgcc ttggtcctc aaagacatg 
      atccagaaa aacttgggt tcggtgggc gaagacgag 
      tgcataatt ctctcctct ttgtgcaac accatctcg 
      ggcctttcg gtcaaacaa gtcgaagac aacgaaacg 
      ttcaacttc ttcgccttc cgcttggac tggttccgc 
      ttgcaggcg tacatgtcc acaggcaac gccctcttg 
      cgcatcatg gacaacaaa gacctggcc cagtttttg 
      gacaccgtg caattccac acgaaaatg gtcgacaat 
      ctggacgaa atgctagtc gagacttcg gatttatca 
      atcttctgc ttctacaac cgcattttc gaggaccag 
      ttccacatg tgtctggag ttcccggcc cagaaccgc 
      ttcatcgtg gccttcccg tcgatctgc tcccacttc 
      cagcactgc acgcacgag ctgtgcccc gaggagcgc 
      caccacatc cgcgaacgc agcctgtcg gtggtcaac 
      gtcttcctg gacgagatg gccaaggaa gccaagaac 
      attatcacg gcgatttgc gacgcccag tgcaagatg 
      agcgacaag ctcctgccc aaaaactgc gcccacttg 
      atctcgcag caaatgaac cgcaagaag aaggagaag 
      aataagaag aacaccctc gagatcgag aaaccgggc 
      aaggagagt tacaggaaa acgagggag aatttgacg 
      actatggat aagttgcac atggctttg accgagctc 
      tgctatgct attaactac ttctcgaat ataaacgtc 
      tgggagtat acgttcgct cctagggaa tacttgcac 
      cagcacttg gagaatcgc tttgcgcgg gctttggtc 
      ggcatggtc atgtatagc ccggacact aacgaaatc 
      gccaaaccg tccgagctt ttggttagc gtccgcgcc 
      tacatgaac gtgctccaa acggtggag aactacgtc 
      catattgac ataacccgg gtttttaac aactgtctc 
      ctgcaacaa acccagcct gtggacagc catggggag 
      aaaaccata gctgcgatt tacacccag tggtattcc 
      gaagtgtta ctgcgacgt gttagcgcc ggcaatata 
      atcttctcg atgaaccaa cgctcgttt gtttgcctc 
      accgccgag gcggcgatt ccgttcaac cccgaggag 
      tattcagac gtgaacgag ctgcgcgcc ctggcggag 
      ctgatcggt ccctacggt atgaagcag ttgagcgag 
      acgctgatg tggcacatc gccagtcaa gtcatcgag 
      ttgaagaaa ctagccgag gccaacaag gacgtcctt 
      ttgctgctc cgcaccaac ttcgacaag cccgaagtg 
      atgaaagaa cagttcaag aagctgacc agcgtcgag 
      aacgtgctc cagcgcatg acgatcgtc ggcgtgatt 
      ctgagcttc cgccagttg gcgcagtcg tgcctcact 
      gatgtcctg gagcagagg atcccgttc ctcctgagc 
      tcgatcctg gatttccgc caccatttg ccgagcggt 
      gacccgctg aaggtcgtg agtgagatg tgctcagcg 
      gcggggatt ccctgcaag gtggatccg acgctggtc 
      aatgcgctc aagttgcag aagccggag gtggacagt 
      gacgagcac ttgatcacc tgtttgctg atggtgttt 
      gtggcggtg tcgattccc aagctggcc aagcaagag 
      cagtcgttt tatagggcg tcgcttgag gggcatacg 
      aataatatt cattgtatg gccttggcg gttaatcat 
      atcttcggg gcgttgttc acgatttgt aatcagggg 
      gatatggag gaccggatg aaggagttt ctggcgttg 
      gcgtcctcg agtttgctc aggttggga caggaggct 
      gataaggag gccacgaaa aatagggag agtgtgtat 
      ttgttgttg gatcagatc gtgcaagag tcgccattt 
      ttgacgatg gatttgttg gagtcgtgc tttccatat 
      gcgctgatc aggaatgcc tatcacgcg gtgtataag 
      caggagcag ttgttggcg taa
    mRNA sequence
    • >TC001541 RA
      tctgacgtt tatctgaca tttcgttaa ccaacaaat 
      caaaatatc tcattttac caaaaaaac gatgaaata 
      acacaattc atcacttaa aaactcacg tccgggtcg 
      aaggaaact aaatcaaaa tggcccgtc ctctcgccc 
      ccagcgagc ggaaaatcg ccgaaaaac tcatcatcc 
      tcaacgaca ggggcatcg gcatgctca cacgcatct 
      acaacatta aaaaagcct gcggagacg ccaaatcca 
      agccggggt ttctctccg acaagaact tggagtctt 
      cgatcaaga gcattgtgc ggcggtttc cgaacgtcg 
      acagcaaga acttgactg cgattcaag ggctcagga 
      acgagataa tcaaatctt tgtcgctgt attactaca 
      cgttcgtcg atttgcttg atttcaaag accacgttt 
      gcgagttgt tgactagca tgggggcgt ttcaaatcg 
      agcttgata tttcgctca atttcgaac tcactaagc 
      attatcttg acttggtga cgacctacg tgtctctga 
      tggtgttgc tgtcgcggg ttgaggacc gcaaggccg 
      ttctggggc tttttaacg ccgcttatg aaatcgtgc 
      attcctcgc ccgattcaa gcttcccgc ggttgggca 
      acatgatca tggagtatg agattccgt ttaaaaagt 
      tatgcgagg agtttgtgc cccacacta agctgcttc 
      taagcgcct tgaactcgc ttctaccca tttatatcg 
      cccggaatg tctcggctg ataaatggc gctctgaat 
      tgaagttga gtctagtag gcaacccag cttcgctcc 
      tcaagccgg tgatagccg accggatgt cgtgcgagt 
      acgtctcgc ttgatacca tggaacgct ggattgtct 
      tcggtttgt tgctaattc acccgcact tgcagcaag 
      atcatttga ataaattat ggatcaatg cgcttgagt 
      cgtcttggg tcttgcccc tgttccggg acgaggtta 
      tctacaccc atcagtaca tctgtgggt actttgata 
      ccatcaagg gctatagta aaagggcgt ccgaggtta 
      aggattgcc acagtcaag cgatacaaa aggctgggt 
      acaaacacc gcgaacgcc gaaaattcc taaggacgg 
      ccctcaaag aattaggct tgattttga tggaccagc 
      cggggcttt tggggccga aggccctca tggtcttca 
      tgggtttgt gctacgcac gggacgagg tcttatggt 
      tgttgcgtc acaacgaca acccacccc aacaaaaaa 
      acaagggta aatcgaccg aggatttag tcgatagac 
      acctgccag aactcctct tccacatgg aagacttac 
      gggtgttgg tccgaaagt acagtcaag tcatgcaaa 
      ggtactacg tccagtacc tctcacact tcgacgcct 
      tggtcctca aagacatga tccagaaaa acttgggtt 
      cggtgggcg aagacgagt gcataattc tctcctctt 
      tgtgcaaca ccatctcgg gcctttcgg tcaaacaag 
      tcgaagaca acgaaacgt tcaacttct tcgccttcc 
      gcttggact ggttccgct tgcaggcgt acatgtcca 
      caggcaacg ccctcttgc gcatcatgg acaacaaag 
      acctggccc agtttttgg acaccgtgc aattccaca 
      cgaaaatgg tcgacaatc tggacgaaa tgctagtcg 
      agacttcgg atttatcaa tcttctgct tctacaacc 
      gcattttcg aggaccagt tccacatgt gtctggagt 
      tcccggccc agaaccgct tcatcgtgg ccttcccgt 
      cgatctgct cccacttcc agcactgca cgcacgagc 
      tgtgccccg aggagcgcc accacatcc gcgaacgca 
      gcctgtcgg tggtcaacg tcttcctgg acgagatgg 
      ccaaggaag ccaagaaca ttatcacgg cgatttgcg 
      acgcccagt gcaagatga gcgacaagc tcctgccca 
      aaaactgcg cccacttga tctcgcagc aaatgaacc 
      gcaagaaga aggagaaga ataagaaga acaccctcg 
      agatcgaga aaccgggca aggagagtt acaggaaaa 
      cgagggaga atttgacga ctatggata agttgcaca 
      tggctttga ccgagctct gctatgcta ttaactact 
      tctcgaata taaacgtct gggagtata cgttcgctc 
      ctagggaat acttgcacc agcacttgg agaatcgct 
      ttgcgcggg ctttggtcg gcatggtca tgtatagcc 
      cggacacta acgaaatcg ccaaaccgt ccgagcttt 
      tggttagcg tccgcgcct acatgaacg tgctccaaa 
      cggtggaga actacgtcc atattgaca taacccggg 
      tttttaaca actgtctcc tgcaacaaa cccagcctg 
      tggacagcc atggggaga aaaccatag ctgcgattt 
      acacccagt ggtattccg aagtgttac tgcgacgtg 
      ttagcgccg gcaatataa tcttctcga tgaaccaac 
      gctcgtttg tttgcctca ccgccgagg cggcgattc 
      cgttcaacc ccgaggagt attcagacg tgaacgagc 
      tgcgcgccc tggcggagc tgatcggtc cctacggta 
      tgaagcagt tgagcgaga cgctgatgt ggcacatcg 
      ccagtcaag tcatcgagt tgaagaaac tagccgagg 
      ccaacaagg acgtccttt tgctgctcc gcaccaact 
      tcgacaagc ccgaagtga tgaaagaac agttcaaga 
      agctgacca gcgtcgaga acgtgctcc agcgcatga 
      cgatcgtcg gcgtgattc tgagcttcc gccagttgg 
      cgcagtcgt gcctcactg atgtcctgg agcagagga 
      tcccgttcc tcctgagct cgatcctgg atttccgcc 
      accatttgc cgagcggtg acccgctga aggtcgtga 
      gtgagatgt gctcagcgg cggggattc cctgcaagg 
      tggatccga cgctggtca atgcgctca agttgcaga 
      agccggagg tggacagtg acgagcact tgatcacct 
      gtttgctga tggtgtttg tggcggtgt cgattccca 
      agctggcca agcaagagc agtcgtttt atagggcgt 
      cgcttgagg ggcatacga ataatattc attgtatgg 
      ccttggcgg ttaatcata tcttcgggg cgttgttca 
      cgatttgta atcaggggg atatggagg accggatga 
      aggagtttc tggcgttgg cgtcctcga gtttgctca 
      ggttgggac aggaggctg ataaggagg ccacgaaaa 
      atagggaga gtgtgtatt tgttgttgg atcagatcg 
      tgcaagagt cgccatttt tgacgatgg atttgttgg 
      agtcgtgct ttccatatg cgctgatca ggaatgcct 
      atcacgcgg tgtataagc aggagcagt tgttggcgt 
      aaattaagt atttatttc ccgtacaaa ttttattca 
      tcatttagt actatcaag tacaacaca attgtatca 
      caattccta tttaattag tttaaatac attatgtta 
      agtaacgag catttatat catgatatt gtagtgtta 
      caacatgta ttgtaaatt tttttatat tctgtacgt 
      cttttattt ttactaaac cattaacac gctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY