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Gene information
General information
TC001525 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 621007..623002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001525
    iBeetle screen iB_00257
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 5

    egg chamber shape irregular - Number of animals showing the phenotype: 5
    egg chamber size decreased - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings slightly blistered - Number of animals showing the phenotype: 9

    Adult morphology
    elytra partially blistered - Number of animals showing the phenotype: 4


    hindwing irregular - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001525 RA
      MSTYEISKS SLLSLKAEI LRKQQELAK AKVENEVKI 
      RQIKKNTPL DLKNKGIEQ RQAQDLSEE DQNLLKKSR 
      EALEAKTRL YDKLSKGAS RLTDDEIEY CRRYLVKFD 
      KKARVSQHY QEEEEEDDP ISDHYDPPK NPDEEWVEY 
      VDCLGRTRT CLRKDLDYL KSKDKDLKL IVEKRRNRD 
      KSRSRSRSR SRTRYRSRS RSRSRPRER SPSPEKGDK 
      SPELTEENE LLSSDMRRE ILRRQWEKE EEEIRNKSD 
      VHYQDLLFN EARTHGVGY YGFSKDEET RAKQQEALK 
      KLREETREK QKKSENLRA MREKQIAAR VRAAMNRKR 
      QRMGLPPLE DEPEPPPTP VEETPQKVD EETSEKEKK 
      LEEKRKKHV RPWDLGKEG VKEHYEYSQ EEWVDKKRK 
      ERPSEFAPP KEYRKERKK EKRYDSDEE TSKFEMRQT 
      TKKSKPVPI VNELSDDES ETCGRGKGA EVAPPSSYN 
      DPRRGRKRP EAPNVSESI EAGLKFLRE QMEKKQTSS 
      RRSEDIFLS
    CDS sequence
    • >TC001525 RA
      atgagtacg tatgaaata agtaaatca tcgctcctc 
      agcttgaaa gcggagatt ttgcgaaaa cagcaagaa 
      ctcgcgaaa gccaaggtt gagaatgag gtcaaaatc 
      aggcaaatt aaaaagaac actcctctt gacctaaag 
      aacaaaggc attgagcag agacaggcc caagatctt 
      agtgaggag gaccaaaat ttgctgaaa aagtccaga 
      gaggccctg gaggcaaaa actcgactt tacgacaaa 
      ctctcaaaa ggtgcttca cgacttaca gacgacgaa 
      atagaatat tgtcgccga tatttggtg aagtttgac 
      aagaaggca cgcgtttct cagcattat caggaggag 
      gaggaggaa gacgaccct atttctgat cattatgac 
      cccccaaag aatccagac gaagaatgg gtggagtac 
      gtggattgt ttggggcga acgcggacg tgcctccgc 
      aaagacttg gattattta aaatcgaaa gacaaagac 
      ttgaaattg atagttgaa aagcgccga aatcgtgac 
      aaaagcaga agcagaagt cgcagcaga agtaggact 
      agatatcgg agtagaagc agaagtaga agtaggccc 
      agagaacgt tcaccgtcc cctgaaaag ggcgacaaa 
      tcaccagaa ctcactgaa gaaaatgaa ctgctttct 
      tccgacatg cggcgcgag attttgcgc cgacagtgg 
      gaaaaagaa gaggaagaa atcaggaac aagtcagat 
      gtgcactac caagatctt ctcttcaac gaggctcgc 
      acgcatggt gttggttac tacggcttt tccaaggac 
      gaagaaact cgagccaag caacaggaa gctttgaag 
      aagctcaga gaagaaacg cgagaaaag cagaagaaa 
      tctgagaat ttgcgcgcg atgagagag aaacaaatc 
      gcagcgcga gtcagagct gcgatgaac agaaaacga 
      cagaggatg ggtttgccg cctttggaa gacgaacct 
      gagccaccc ccaacccct gttgaagaa accccacaa 
      aaagtggac gaagaaacg agcgaaaaa gagaagaaa 
      cttgaggag aaacgaaag aaacacgtc agaccatgg 
      gatttgggg aaagaaggg gtgaaggaa cattacgag 
      tacagtcaa gaggaatgg gttgataaa aaacggaaa 
      gagcgtcca agtgaattc gcgcccccg aaagagtat 
      agaaaggag cgcaaaaaa gagaagaga tatgacagt 
      gatgaggaa acttccaaa ttcgagatg agacaaacg 
      accaagaaa tctaaacca gtccccatt gtgaacgag 
      ttaagtgat gacgaaagt gagacttgt ggtaggggc 
      aagggggcc gaagtggcc ccaccgtcg agttacaac 
      gacccgaga agggggcgt aaacgtccg gaagccccc 
      aacgtgagt gagtcgatt gaagccggc ttgaaattt 
      ctacgagaa cagatggaa aagaagcaa actagtagt 
      cgcagatct gaggatatt ttcctttcg tga
    mRNA sequence
    • >TC001525 RA
      cttgtcttc actgacata aataactgt cactgtcac 
      tttgacaaa tactttcta gtaaacaaa ttgggacta 
      aagggcctt taaaaaacc ttatttata gagaatttt 
      ccataaata cgaactaaa tcacataat taacaaaat 
      gagtacgta tgaaataag taaatcatc gctcctcag 
      cttgaaagc ggagatttt gcgaaaaca gcaagaact 
      cgcgaaagc caaggttga gaatgaggt caaaatcag 
      gcaaattaa aaagaacac tcctcttga cctaaagaa 
      caaaggcat tgagcagag acaggccca agatcttag 
      tgaggagga ccaaaattt gctgaaaaa gtccagaga 
      ggccctgga ggcaaaaac tcgacttta cgacaaact 
      ctcaaaagg tgcttcacg acttacaga cgacgaaat 
      agaatattg tcgccgata tttggtgaa gtttgacaa 
      gaaggcacg cgtttctca gcattatca ggaggagga 
      ggaggaaga cgaccctat ttctgatca ttatgaccc 
      cccaaagaa tccagacga agaatgggt ggagtacgt 
      ggattgttt ggggcgaac gcggacgtg cctccgcaa 
      agacttgga ttatttaaa atcgaaaga caaagactt 
      gaaattgat agttgaaaa gcgccgaaa tcgtgacaa 
      aagcagaag cagaagtcg cagcagaag taggactag 
      atatcggag tagaagcag aagtagaag taggcccag 
      agaacgttc accgtcccc tgaaaaggg cgacaaatc 
      accagaact cactgaaga aaatgaact gctttcttc 
      cgacatgcg gcgcgagat tttgcgccg acagtggga 
      aaaagaaga ggaagaaat caggaacaa gtcagatgt 
      gcactacca agatcttct cttcaacga ggctcgcac 
      gcatggtgt tggttacta cggcttttc caaggacga 
      agaaactcg agccaagca acaggaagc tttgaagaa 
      gctcagaga agaaacgcg agaaaagca gaagaaatc 
      tgagaattt gcgcgcgat gagagagaa acaaatcgc 
      agcgcgagt cagagctgc gatgaacag aaaacgaca 
      gaggatggg tttgccgcc tttggaaga cgaacctga 
      gccaccccc aacccctgt tgaagaaac cccacaaaa 
      agtggacga agaaacgag cgaaaaaga gaagaaact 
      tgaggagaa acgaaagaa acacgtcag accatggga 
      tttggggaa agaaggggt gaaggaaca ttacgagta 
      cagtcaaga ggaatgggt tgataaaaa acggaaaga 
      gcgtccaag tgaattcgc gcccccgaa agagtatag 
      aaaggagcg caaaaaaga gaagagata tgacagtga 
      tgaggaaac ttccaaatt cgagatgag acaaacgac 
      caagaaatc taaaccagt ccccattgt gaacgagtt 
      aagtgatga cgaaagtga gacttgtgg taggggcaa 
      gggggccga agtggcccc accgtcgag ttacaacga 
      cccgagaag ggggcgtaa acgtccgga agcccccaa 
      cgtgagtga gtcgattga agccggctt gaaatttct 
      acgagaaca gatggaaaa gaagcaaac tagtagtcg 
      cagatctga ggatatttt cctttcgtg aaataaaac 
      tacttgttt gta