| General information | |||
| TC001510 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 2436687..2440989 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001510 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00256 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
- >TC001510 RA
MCEPNLSDE SLTEKFKRE FNEQFHILV RMHLSFLLD QATNEHETL SEKNRLKKW SLVPFNKKK SKGALDGAP LTQEGICQV YQLIEFLKK EQNIKVEGV FRRTGSLAR QQELRNLLS RGVTLELEG GTYSIHDCA SVLKGFLAE LPDPLLTEA HYPAYCQIA ELCVNKEKT VQEEKLLAA LQLLLLLLP NENKVLLKD ILDLLNFTA SFESFNRMS ADNLAKLFT PHLLCPRKL TPEQLHVTS QTLAGIVSF MIVKNSELF KIPQNLILE FRARYEKRR ALSANCLNE SATDQCAAS TVFTFVDQE RTAKENETN TTETALAQL YAHIQSLPE SSKKRKLVK QFNKENGHG TPLQVLRSG VQKNKSLGD SIKKHIFHK SVVKSLKKR VSSEENLHT PPLQTALAR ARLFCTNCD SSPDQVASD EPQSKKIRR STSETEGET PDAETGIGI TSTPACVAL HFTPDDQER KSMSPITRS TQRMTRAMQ ETMMTPRSR KPVLLVSGT NINTLAKIE TVEAMDEVP EEVEKDNSL SQTFREYLE NRSMLTTSS PAADSSFSS RTDDFNSDL SPSKMTDSL LSCLDGNDP GSEKELVLK PKQFDENGQ PIVFETSF
- >TC001510 RA
atgtgtgaa ccaaatttg agtgacgag agtttgact gaaaagttc aaaagggag tttaacgag cagttccat attttagtt cggatgcac ttatcgttt ttgctggac caggccact aatgaacat gagacttta tctgagaaa aatcgcttg aaaaagtgg tctctagtc ccgtttaat aaaaagaag agtaagggg gccctggac ggggcaccc ttgacccag gagggcatt tgtcaggtg taccaattg atcgagttt ttgaaaaag gagcaaaat attaaagtt gagggcgtt ttccggcga acggggtct ttggcgcgc caacaagaa ttacgtaat ttactaagc cggggggtc actttagag cttgaaggg ggcacctac agtatccac gattgtgcc tcggttttg aagggtttt ttggcggaa ttgcccgac cctttactc accgaagcc cactatccg gcttattgc caaatcgcc gaattgtgt gtcaataag gaaaaaacg gtgcaagaa gaaaagctt ttggctgct ttacaactc ctcctcctc ctgctcccc aacgaaaat aaagttcta ttgaaagac atcctcgat ttgttgaat ttcaccgct tctttcgaa tctttcaac agaatgtct gctgataat ttagccaaa cttttcacg ccccatctt ttgtgcccc cggaaactc accccggaa caactgcac gtcacttcg caaactctc gccggaatt gtgtcgttc atgattgtg aaaaattca gagttgttt aaaattccg caaaatttg atactggaa tttcgggcg cgttatgag aaacgtcgg gcgttgtct gcgaattgt ctcaacgaa tcggcgaca gatcagtgt gctgcgagc acagttttt acgtttgtc gaccaggaa cggactgcc aaagaaaat gaaaccaat acaactgag acggcttta gcccagctt tatgctcat attcagagt cttccagag tcgtcgaaa aaacgcaaa ttggttaaa cagtttaat aaggagaat ggacacggg acgccactg caagtgcta aggagtggc gttcagaaa aataagagt ttgggggat tcgattaag aagcatatt tttcacaaa agtgtcgtc aagagtttg aagaagagg gtaagcagc gaggaaaat ttgcatact ccccccctt cagacagcc ctagctcgc gcccgcctt ttctgtaca aactgcgac tcaagcccc gatcaagtc gccagtgac gaaccccaa tcgaaaaaa atccgtcga tcgactagc gaaactgaa ggtgaaacg cctgatgcc gaaacaggt atcggaata acgagcacg ccagcatgc gttgctctg catttcacg cccgacgac caggagcgt aagtccatg tcacccata accagatcc acacagcgt atgaccaga gccatgcag gaaactatg atgactccc agaagcagg aagccggtc ttgcttgtt tccgggacg aatatcaac accttggcg aagattgaa acagttgag gccatggat gaagtcccc gaggaggtg gagaaggat aattcgttg agtcagacg tttcgagag tatttggaa aatcggagc atgttgacg acttcgtcg cccgccgca gattcgagt ttttccagt cggactgat gatttcaac tccgacttg agtccgagc aaaatgact gattcgtta ctgagttgt ttggacgga aatgacccc ggaagtgag aaggaactt gttctcaaa ccgaaacaa ttcgatgaa aatggacaa cccatagta tttgaaacc tcattttag
- >TC001510 RA
taatttctg tcaaacaat tcaaaaagt attatttat ttagcaatt ttatcccaa atggtgcta ttttatgat taaatatta cacgatagt ttacaattg tacacaata tgtgtaaat ttagtgcga gtttgagca acgtgtggt gattttcaa acctaacct caatcaatt caaagtgtt caaaatgtg tgaaccaaa tttgagtga cgagagttt gactgaaaa gttcaaaag ggagtttaa cgagcagtt ccatatttt agttcggat gcacttatc gtttttgct ggaccaggc cactaatga acatgagac tttatctga gaaaaatcg cttgaaaaa gtggtctct agtcccgtt taataaaaa gaagagtaa gggggccct ggacggggc acccttgac ccaggaggg catttgtca ggtgtacca attgatcga gtttttgaa aaaggagca aaatattaa agttgaggg cgttttccg gcgaacggg gtctttggc gcgccaaca agaattacg taatttact aagccgggg ggtcacttt agagcttga agggggcac ctacagtat ccacgattg tgcctcggt tttgaaggg ttttttggc ggaattgcc cgacccttt actcaccga agcccacta tccggctta ttgccaaat cgccgaatt gtgtgtcaa taaggaaaa aacggtgca agaagaaaa gcttttggc tgctttaca actcctcct cctcctgct ccccaacga aaataaagt tctattgaa agacatcct cgatttgtt gaatttcac cgcttcttt cgaatcttt caacagaat gtctgctga taatttagc caaactttt cacgcccca tcttttgtg cccccggaa actcacccc ggaacaact gcacgtcac ttcgcaaac tctcgccgg aattgtgtc gttcatgat tgtgaaaaa ttcagagtt gtttaaaat tccgcaaaa tttgatact ggaatttcg ggcgcgtta tgagaaacg tcgggcgtt gtctgcgaa ttgtctcaa cgaatcggc gacagatca gtgtgctgc gagcacagt ttttacgtt tgtcgacca ggaacggac tgccaaaga aaatgaaac caatacaac tgagacggc tttagccca gctttatgc tcatattca gagtcttcc agagtcgtc gaaaaaacg caaattggt taaacagtt taataagga gaatggaca cgggacgcc actgcaagt gctaaggag tggcgttca gaaaaataa gagtttggg ggattcgat taagaagca tatttttca caaaagtgt cgtcaagag tttgaagaa gagggtaag cagcgagga aaatttgca tactccccc ccttcagac agccctagc tcgcgcccg ccttttctg tacaaactg cgactcaag ccccgatca agtcgccag tgacgaacc ccaatcgaa aaaaatccg tcgatcgac tagcgaaac tgaaggtga aacgcctga tgccgaaac aggtatcgg aataacgag cacgccagc atgcgttgc tctgcattt cacgcccga cgaccagga gcgtaagtc catgtcacc cataaccag atccacaca gcgtatgac cagagccat gcaggaaac tatgatgac tcccagaag caggaagcc ggtcttgct tgtttccgg gacgaatat caacacctt ggcgaagat tgaaacagt tgaggccat ggatgaagt ccccgagga ggtggagaa ggataattc gttgagtca gacgtttcg agagtattt ggaaaatcg gagcatgtt gacgacttc gtcgcccgc cgcagattc gagtttttc cagtcggac tgatgattt caactccga cttgagtcc gagcaaaat gactgattc gttactgag ttgtttgga cggaaatga ccccggaag tgagaagga acttgttct caaaccgaa acaattcga tgaaaatgg acaacccat agtatttga aacctcatt ttagttaat tgtagtaca aaagcagga ccattttgt aaatatatt tattaaaga aag
