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Gene information
General information
TC001510 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2436687..2440989
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001510
    iBeetle screen iB_00256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001510 RA
      MCEPNLSDE SLTEKFKRE FNEQFHILV RMHLSFLLD 
QATNEHETL SEKNRLKKW SLVPFNKKK SKGALDGAP 
LTQEGICQV YQLIEFLKK EQNIKVEGV FRRTGSLAR 
QQELRNLLS RGVTLELEG GTYSIHDCA SVLKGFLAE 
LPDPLLTEA HYPAYCQIA ELCVNKEKT VQEEKLLAA 
LQLLLLLLP NENKVLLKD ILDLLNFTA SFESFNRMS 
ADNLAKLFT PHLLCPRKL TPEQLHVTS QTLAGIVSF 
MIVKNSELF KIPQNLILE FRARYEKRR ALSANCLNE 
SATDQCAAS TVFTFVDQE RTAKENETN TTETALAQL 
YAHIQSLPE SSKKRKLVK QFNKENGHG TPLQVLRSG 
VQKNKSLGD SIKKHIFHK SVVKSLKKR VSSEENLHT 
PPLQTALAR ARLFCTNCD SSPDQVASD EPQSKKIRR 
STSETEGET PDAETGIGI TSTPACVAL HFTPDDQER 
KSMSPITRS TQRMTRAMQ ETMMTPRSR KPVLLVSGT 
NINTLAKIE TVEAMDEVP EEVEKDNSL SQTFREYLE 
NRSMLTTSS PAADSSFSS RTDDFNSDL SPSKMTDSL 
LSCLDGNDP GSEKELVLK PKQFDENGQ PIVFETSF
    CDS sequence
    • >TC001510 RA
      atgtgtgaa ccaaatttg agtgacgag agtttgact 
gaaaagttc aaaagggag tttaacgag cagttccat 
attttagtt cggatgcac ttatcgttt ttgctggac 
caggccact aatgaacat gagacttta tctgagaaa 
aatcgcttg aaaaagtgg tctctagtc ccgtttaat 
aaaaagaag agtaagggg gccctggac ggggcaccc 
ttgacccag gagggcatt tgtcaggtg taccaattg 
atcgagttt ttgaaaaag gagcaaaat attaaagtt 
gagggcgtt ttccggcga acggggtct ttggcgcgc 
caacaagaa ttacgtaat ttactaagc cggggggtc 
actttagag cttgaaggg ggcacctac agtatccac 
gattgtgcc tcggttttg aagggtttt ttggcggaa 
ttgcccgac cctttactc accgaagcc cactatccg 
gcttattgc caaatcgcc gaattgtgt gtcaataag 
gaaaaaacg gtgcaagaa gaaaagctt ttggctgct 
ttacaactc ctcctcctc ctgctcccc aacgaaaat 
aaagttcta ttgaaagac atcctcgat ttgttgaat 
ttcaccgct tctttcgaa tctttcaac agaatgtct 
gctgataat ttagccaaa cttttcacg ccccatctt 
ttgtgcccc cggaaactc accccggaa caactgcac 
gtcacttcg caaactctc gccggaatt gtgtcgttc 
atgattgtg aaaaattca gagttgttt aaaattccg 
caaaatttg atactggaa tttcgggcg cgttatgag 
aaacgtcgg gcgttgtct gcgaattgt ctcaacgaa 
tcggcgaca gatcagtgt gctgcgagc acagttttt 
acgtttgtc gaccaggaa cggactgcc aaagaaaat 
gaaaccaat acaactgag acggcttta gcccagctt 
tatgctcat attcagagt cttccagag tcgtcgaaa 
aaacgcaaa ttggttaaa cagtttaat aaggagaat 
ggacacggg acgccactg caagtgcta aggagtggc 
gttcagaaa aataagagt ttgggggat tcgattaag 
aagcatatt tttcacaaa agtgtcgtc aagagtttg 
aagaagagg gtaagcagc gaggaaaat ttgcatact 
ccccccctt cagacagcc ctagctcgc gcccgcctt 
ttctgtaca aactgcgac tcaagcccc gatcaagtc 
gccagtgac gaaccccaa tcgaaaaaa atccgtcga 
tcgactagc gaaactgaa ggtgaaacg cctgatgcc 
gaaacaggt atcggaata acgagcacg ccagcatgc 
gttgctctg catttcacg cccgacgac caggagcgt 
aagtccatg tcacccata accagatcc acacagcgt 
atgaccaga gccatgcag gaaactatg atgactccc 
agaagcagg aagccggtc ttgcttgtt tccgggacg 
aatatcaac accttggcg aagattgaa acagttgag 
gccatggat gaagtcccc gaggaggtg gagaaggat 
aattcgttg agtcagacg tttcgagag tatttggaa 
aatcggagc atgttgacg acttcgtcg cccgccgca 
gattcgagt ttttccagt cggactgat gatttcaac 
tccgacttg agtccgagc aaaatgact gattcgtta 
ctgagttgt ttggacgga aatgacccc ggaagtgag 
aaggaactt gttctcaaa ccgaaacaa ttcgatgaa 
aatggacaa cccatagta tttgaaacc tcattttag
    mRNA sequence
    • >TC001510 RA
      taatttctg tcaaacaat tcaaaaagt attatttat 
ttagcaatt ttatcccaa atggtgcta ttttatgat 
taaatatta cacgatagt ttacaattg tacacaata 
tgtgtaaat ttagtgcga gtttgagca acgtgtggt 
gattttcaa acctaacct caatcaatt caaagtgtt 
caaaatgtg tgaaccaaa tttgagtga cgagagttt 
gactgaaaa gttcaaaag ggagtttaa cgagcagtt 
ccatatttt agttcggat gcacttatc gtttttgct 
ggaccaggc cactaatga acatgagac tttatctga 
gaaaaatcg cttgaaaaa gtggtctct agtcccgtt 
taataaaaa gaagagtaa gggggccct ggacggggc 
acccttgac ccaggaggg catttgtca ggtgtacca 
attgatcga gtttttgaa aaaggagca aaatattaa 
agttgaggg cgttttccg gcgaacggg gtctttggc 
gcgccaaca agaattacg taatttact aagccgggg 
ggtcacttt agagcttga agggggcac ctacagtat 
ccacgattg tgcctcggt tttgaaggg ttttttggc 
ggaattgcc cgacccttt actcaccga agcccacta 
tccggctta ttgccaaat cgccgaatt gtgtgtcaa 
taaggaaaa aacggtgca agaagaaaa gcttttggc 
tgctttaca actcctcct cctcctgct ccccaacga 
aaataaagt tctattgaa agacatcct cgatttgtt 
gaatttcac cgcttcttt cgaatcttt caacagaat 
gtctgctga taatttagc caaactttt cacgcccca 
tcttttgtg cccccggaa actcacccc ggaacaact 
gcacgtcac ttcgcaaac tctcgccgg aattgtgtc 
gttcatgat tgtgaaaaa ttcagagtt gtttaaaat 
tccgcaaaa tttgatact ggaatttcg ggcgcgtta 
tgagaaacg tcgggcgtt gtctgcgaa ttgtctcaa 
cgaatcggc gacagatca gtgtgctgc gagcacagt 
ttttacgtt tgtcgacca ggaacggac tgccaaaga 
aaatgaaac caatacaac tgagacggc tttagccca 
gctttatgc tcatattca gagtcttcc agagtcgtc 
gaaaaaacg caaattggt taaacagtt taataagga 
gaatggaca cgggacgcc actgcaagt gctaaggag 
tggcgttca gaaaaataa gagtttggg ggattcgat 
taagaagca tatttttca caaaagtgt cgtcaagag 
tttgaagaa gagggtaag cagcgagga aaatttgca 
tactccccc ccttcagac agccctagc tcgcgcccg 
ccttttctg tacaaactg cgactcaag ccccgatca 
agtcgccag tgacgaacc ccaatcgaa aaaaatccg 
tcgatcgac tagcgaaac tgaaggtga aacgcctga 
tgccgaaac aggtatcgg aataacgag cacgccagc 
atgcgttgc tctgcattt cacgcccga cgaccagga 
gcgtaagtc catgtcacc cataaccag atccacaca 
gcgtatgac cagagccat gcaggaaac tatgatgac 
tcccagaag caggaagcc ggtcttgct tgtttccgg 
gacgaatat caacacctt ggcgaagat tgaaacagt 
tgaggccat ggatgaagt ccccgagga ggtggagaa 
ggataattc gttgagtca gacgtttcg agagtattt 
ggaaaatcg gagcatgtt gacgacttc gtcgcccgc 
cgcagattc gagtttttc cagtcggac tgatgattt 
caactccga cttgagtcc gagcaaaat gactgattc 
gttactgag ttgtttgga cggaaatga ccccggaag 
tgagaagga acttgttct caaaccgaa acaattcga 
tgaaaatgg acaacccat agtatttga aacctcatt 
ttagttaat tgtagtaca aaagcagga ccattttgt 
aaatatatt tattaaaga aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY