Status
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Gene information
General information
TC001510 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2436687..2440989
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001510
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001510 RA
      MCEPNLSDE SLTEKFKRE FNEQFHILV RMHLSFLLD 
      QATNEHETL SEKNRLKKW SLVPFNKKK SKGALDGAP 
      LTQEGICQV YQLIEFLKK EQNIKVEGV FRRTGSLAR 
      QQELRNLLS RGVTLELEG GTYSIHDCA SVLKGFLAE 
      LPDPLLTEA HYPAYCQIA ELCVNKEKT VQEEKLLAA 
      LQLLLLLLP NENKVLLKD ILDLLNFTA SFESFNRMS 
      ADNLAKLFT PHLLCPRKL TPEQLHVTS QTLAGIVSF 
      MIVKNSELF KIPQNLILE FRARYEKRR ALSANCLNE 
      SATDQCAAS TVFTFVDQE RTAKENETN TTETALAQL 
      YAHIQSLPE SSKKRKLVK QFNKENGHG TPLQVLRSG 
      VQKNKSLGD SIKKHIFHK SVVKSLKKR VSSEENLHT 
      PPLQTALAR ARLFCTNCD SSPDQVASD EPQSKKIRR 
      STSETEGET PDAETGIGI TSTPACVAL HFTPDDQER 
      KSMSPITRS TQRMTRAMQ ETMMTPRSR KPVLLVSGT 
      NINTLAKIE TVEAMDEVP EEVEKDNSL SQTFREYLE 
      NRSMLTTSS PAADSSFSS RTDDFNSDL SPSKMTDSL 
      LSCLDGNDP GSEKELVLK PKQFDENGQ PIVFETSF
    CDS sequence
    • >TC001510 RA
      atgtgtgaa ccaaatttg agtgacgag agtttgact 
      gaaaagttc aaaagggag tttaacgag cagttccat 
      attttagtt cggatgcac ttatcgttt ttgctggac 
      caggccact aatgaacat gagacttta tctgagaaa 
      aatcgcttg aaaaagtgg tctctagtc ccgtttaat 
      aaaaagaag agtaagggg gccctggac ggggcaccc 
      ttgacccag gagggcatt tgtcaggtg taccaattg 
      atcgagttt ttgaaaaag gagcaaaat attaaagtt 
      gagggcgtt ttccggcga acggggtct ttggcgcgc 
      caacaagaa ttacgtaat ttactaagc cggggggtc 
      actttagag cttgaaggg ggcacctac agtatccac 
      gattgtgcc tcggttttg aagggtttt ttggcggaa 
      ttgcccgac cctttactc accgaagcc cactatccg 
      gcttattgc caaatcgcc gaattgtgt gtcaataag 
      gaaaaaacg gtgcaagaa gaaaagctt ttggctgct 
      ttacaactc ctcctcctc ctgctcccc aacgaaaat 
      aaagttcta ttgaaagac atcctcgat ttgttgaat 
      ttcaccgct tctttcgaa tctttcaac agaatgtct 
      gctgataat ttagccaaa cttttcacg ccccatctt 
      ttgtgcccc cggaaactc accccggaa caactgcac 
      gtcacttcg caaactctc gccggaatt gtgtcgttc 
      atgattgtg aaaaattca gagttgttt aaaattccg 
      caaaatttg atactggaa tttcgggcg cgttatgag 
      aaacgtcgg gcgttgtct gcgaattgt ctcaacgaa 
      tcggcgaca gatcagtgt gctgcgagc acagttttt 
      acgtttgtc gaccaggaa cggactgcc aaagaaaat 
      gaaaccaat acaactgag acggcttta gcccagctt 
      tatgctcat attcagagt cttccagag tcgtcgaaa 
      aaacgcaaa ttggttaaa cagtttaat aaggagaat 
      ggacacggg acgccactg caagtgcta aggagtggc 
      gttcagaaa aataagagt ttgggggat tcgattaag 
      aagcatatt tttcacaaa agtgtcgtc aagagtttg 
      aagaagagg gtaagcagc gaggaaaat ttgcatact 
      ccccccctt cagacagcc ctagctcgc gcccgcctt 
      ttctgtaca aactgcgac tcaagcccc gatcaagtc 
      gccagtgac gaaccccaa tcgaaaaaa atccgtcga 
      tcgactagc gaaactgaa ggtgaaacg cctgatgcc 
      gaaacaggt atcggaata acgagcacg ccagcatgc 
      gttgctctg catttcacg cccgacgac caggagcgt 
      aagtccatg tcacccata accagatcc acacagcgt 
      atgaccaga gccatgcag gaaactatg atgactccc 
      agaagcagg aagccggtc ttgcttgtt tccgggacg 
      aatatcaac accttggcg aagattgaa acagttgag 
      gccatggat gaagtcccc gaggaggtg gagaaggat 
      aattcgttg agtcagacg tttcgagag tatttggaa 
      aatcggagc atgttgacg acttcgtcg cccgccgca 
      gattcgagt ttttccagt cggactgat gatttcaac 
      tccgacttg agtccgagc aaaatgact gattcgtta 
      ctgagttgt ttggacgga aatgacccc ggaagtgag 
      aaggaactt gttctcaaa ccgaaacaa ttcgatgaa 
      aatggacaa cccatagta tttgaaacc tcattttag
    mRNA sequence
    • >TC001510 RA
      taatttctg tcaaacaat tcaaaaagt attatttat 
      ttagcaatt ttatcccaa atggtgcta ttttatgat 
      taaatatta cacgatagt ttacaattg tacacaata 
      tgtgtaaat ttagtgcga gtttgagca acgtgtggt 
      gattttcaa acctaacct caatcaatt caaagtgtt 
      caaaatgtg tgaaccaaa tttgagtga cgagagttt 
      gactgaaaa gttcaaaag ggagtttaa cgagcagtt 
      ccatatttt agttcggat gcacttatc gtttttgct 
      ggaccaggc cactaatga acatgagac tttatctga 
      gaaaaatcg cttgaaaaa gtggtctct agtcccgtt 
      taataaaaa gaagagtaa gggggccct ggacggggc 
      acccttgac ccaggaggg catttgtca ggtgtacca 
      attgatcga gtttttgaa aaaggagca aaatattaa 
      agttgaggg cgttttccg gcgaacggg gtctttggc 
      gcgccaaca agaattacg taatttact aagccgggg 
      ggtcacttt agagcttga agggggcac ctacagtat 
      ccacgattg tgcctcggt tttgaaggg ttttttggc 
      ggaattgcc cgacccttt actcaccga agcccacta 
      tccggctta ttgccaaat cgccgaatt gtgtgtcaa 
      taaggaaaa aacggtgca agaagaaaa gcttttggc 
      tgctttaca actcctcct cctcctgct ccccaacga 
      aaataaagt tctattgaa agacatcct cgatttgtt 
      gaatttcac cgcttcttt cgaatcttt caacagaat 
      gtctgctga taatttagc caaactttt cacgcccca 
      tcttttgtg cccccggaa actcacccc ggaacaact 
      gcacgtcac ttcgcaaac tctcgccgg aattgtgtc 
      gttcatgat tgtgaaaaa ttcagagtt gtttaaaat 
      tccgcaaaa tttgatact ggaatttcg ggcgcgtta 
      tgagaaacg tcgggcgtt gtctgcgaa ttgtctcaa 
      cgaatcggc gacagatca gtgtgctgc gagcacagt 
      ttttacgtt tgtcgacca ggaacggac tgccaaaga 
      aaatgaaac caatacaac tgagacggc tttagccca 
      gctttatgc tcatattca gagtcttcc agagtcgtc 
      gaaaaaacg caaattggt taaacagtt taataagga 
      gaatggaca cgggacgcc actgcaagt gctaaggag 
      tggcgttca gaaaaataa gagtttggg ggattcgat 
      taagaagca tatttttca caaaagtgt cgtcaagag 
      tttgaagaa gagggtaag cagcgagga aaatttgca 
      tactccccc ccttcagac agccctagc tcgcgcccg 
      ccttttctg tacaaactg cgactcaag ccccgatca 
      agtcgccag tgacgaacc ccaatcgaa aaaaatccg 
      tcgatcgac tagcgaaac tgaaggtga aacgcctga 
      tgccgaaac aggtatcgg aataacgag cacgccagc 
      atgcgttgc tctgcattt cacgcccga cgaccagga 
      gcgtaagtc catgtcacc cataaccag atccacaca 
      gcgtatgac cagagccat gcaggaaac tatgatgac 
      tcccagaag caggaagcc ggtcttgct tgtttccgg 
      gacgaatat caacacctt ggcgaagat tgaaacagt 
      tgaggccat ggatgaagt ccccgagga ggtggagaa 
      ggataattc gttgagtca gacgtttcg agagtattt 
      ggaaaatcg gagcatgtt gacgacttc gtcgcccgc 
      cgcagattc gagtttttc cagtcggac tgatgattt 
      caactccga cttgagtcc gagcaaaat gactgattc 
      gttactgag ttgtttgga cggaaatga ccccggaag 
      tgagaagga acttgttct caaaccgaa acaattcga 
      tgaaaatgg acaacccat agtatttga aacctcatt 
      ttagttaat tgtagtaca aaagcagga ccattttgt 
      aaatatatt tattaaaga aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY