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Gene information
General information
TC001467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 3683664..3694104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001467
    iBeetle screen iB_00253
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001467 RA
      MATIDGRPA QYGITLKQL RDLMEHRGR EGVNKIADF 
      GGVQEICKK LYTSPSEGL SGSQVDLEH RRETFGSNS 
      IPPKPPKTF LQLVWEALQ DITLIILEV AAIVSLGLS 
      FYQPQQEDV PFDDDETSH GWIEGLAIL ISVIVVVLV 
      TAFNDYTKE RQFRGLQSR IEGEHKFAV IRQAEVKQV 
      SVSDIVVGD ICQIKYGDL LPADGILIQ SNDLKVDES 
      SLTGESDHV KKGENYDPM VLSGTHVME GSGKMLVTA 
      VGVNSQAGI IFTLLGAAV DEQEAEIKK MKKEAKKQR 
      KKKSLTGAD DENVTGNSH MNSPAPVPN KLNESKQES 
      KENHVSSPP ASAESHKKE KSVLQAKLT KLAIQIGYA 
      GSTIAVLTV VILIIQFCV KTYVVEGNS WQKNHASHL 
      VRHLIIGVT VLVVAVPEG LPLAVTLSL AYSVKKMMK 
      DNNLVRHLD ACETMGNAT AICSDKTGT LTTNRMTVV 
      QSYICEQLC KSMPKFSDI PAHVGNAIL QGIAVNCAY 
      TSRVMPPDD PTDLPKQVG NKTECALLG FVLGLGKNY 
      QTIRDDYPE ESFTRVYTF NSVRKSMST VIPRAGGGY 
      RLYTKGASE MILNKCAFI YGHDGRLEK FTRDMQERL 
      LKQVIEPMA CDGLRTICI AFREFVPGK AEINQVHIE 
      NEPNWDDED NIVNNLTCL CVVGIEDPV RPEVPDAIR 
      KCQKAGITV RMVTGDNLN TARSIATKC GIVKPNEDF 
      LIIEGKEFN RRIRDSTGE VQQHLLDKV WPKLRVLAR 
      SSPTDKYTL VKGIIDSKV NENREVVAV TGDGTNDGP 
      ALKKADVGF AMGIAGTDV AKEASDIIL TDDNFSSIV 
      KAVMWGRNV YDSIAKFLQ FQLTVNVVA VVVAFIGAC 
      AVQDSPLKA VQMLWVNLI MDTLASLAL ATELPTNDL 
      LLRKPYGRT KPLISRTMM KNILGQAVY QLTVIFALL 
      FVGDKLLDI ESGRGTDLG AGPTQHFTV IFNSFVMMT 
      LFNEFNARK IHGQRNVFE GIFTNPIFY TIWIGTCVS 
      QILIIQYGK MAFATKSLT LEQWLWCLF FGLGTLLWG 
      QLVTTVPTR KIPKILSWG RGHPEEYTE AIAIGEEKF 
      DVDSDKKPR AGQILWIRG LTRLQTQLR VIRAFKSTL 
      EDLEERRSV HSLHSLHSL RSSRSHTGP WPPRPLSDI 
      TYIDEDPTA NKLSPQPSK NERDDHRLL SPNTLKLPP 
      HNPQYNAQH LAPSSANNS DLPKPVHET RI
    CDS sequence
    • >TC001467 RA
      atggctacg atagacggc cgtcccgcg caatatgga 
      attaccctg aaacagtta cgcgacctc atggaacat 
      cgaggtcgc gaaggagta aataaaatt gctgacttt 
      ggaggagta caagaaatt tgtaagaaa ttatacaca 
      tcgcccagt gaaggtctt agcgggtca caggtggac 
      cttgaacat agaagagaa acattcgga tcaaactca 
      attcctccc aaacctcca aaaactttt cttcaatta 
      gtatgggaa gctttacaa gacatcact cttattatt 
      ctggaagta gcagccatt gtgtcttta ggtctttct 
      ttctatcag ccgcaacaa gaagatgtc ccttttgac 
      gatgatgaa actagtcat ggttggatt gagggttta 
      gctatttta atctccgtt atcgtagta gtcttagta 
      acagcattt aacgattat acgaaagaa agacaattc 
      agaggtctt cagagtcga atcgaggga gaacataaa 
      tttgctgtg attcgacaa gctgaagta aaacaagtt 
      tccgttagc gacatagtt gtaggtgat atttgccag 
      ataaaatac ggtgatctt ttaccggca gacggcatc 
      ctaatccaa tccaatgac ctcaaggtg gacgaatct 
      tctcttacg ggcgagtca gaccatgtc aaaaagggc 
      gaaaactac gaccctatg gtcttgtct ggcacccac 
      gtgatggaa ggttcagga aaaatgttg gttactgcg 
      gttggtgtc aactcccaa gcaggtatc atcttcaca 
      ctactagga gcagcagtt gatgaacag gaagccgaa 
      attaagaaa atgaagaaa gaagctaaa aagcagcgg 
      aagaagaaa agtctaaca ggtgctgac gatgaaaac 
      gtaactggt aacagtcat atgaattct cccgctccg 
      gttccaaat aagcttaac gagagtaaa caagaatcc 
      aaagaaaat cacgtatcg tcaccaccg gcgtcggcg 
      gaaagtcac aagaaagaa aagtcggtt cttcaagca 
      aaattgacg aaacttgcc attcagatt ggatatgcc 
      ggttctaca attgccgtt ctcactgtt gtaattttg 
      ataattcag ttttgcgtt aaaacctac gttgttgag 
      ggcaattcg tggcaaaag aatcacgcc agccacttg 
      gtgcgtcat ttgatcatc ggtgtaact gtactcgtg 
      gtggcagta ccagaaggt ttgccgctc gctgttacg 
      ctttcttta gcttatagt gtcaagaaa atgatgaaa 
      gataacaat ttggtaaga catttggac gcttgcgaa 
      accatgggt aatgccact gcaatttgt tcggacaaa 
      accggaact ttgaccacc aatcgcatg accgttgtg 
      caatcctac atttgtgag cagttgtgt aaatccatg 
      ccgaaattt tctgatatt cctgcacat gtcggaaat 
      gcgatcctc cagggcatt gcggttaat tgcgcttac 
      acatcgcga gttatgcct ccggatgat ccgaccgac 
      ttgcccaag caagttggt aacaaaact gagtgcgca 
      ttgttggga ttcgtcctc ggtctcggc aagaactac 
      caaacgatc cgcgatgac tatcctgag gagagtttt 
      acccgcgtt tatacattt aactctgtg agaaaatcc 
      atgagcact gtcatcccc agagcgggt ggtggatat 
      cgattgtac acaaaaggt gcttctgag atgatttta 
      aacaagtgt gccttcatt tacggtcac gacggccgt 
      ttagaaaag ttcaccaga gatatgcaa gagcgtttg 
      ttgaagcaa gttatcgaa ccaatggct tgtgatggt 
      cttcggacg atctgtatc gctttccgc gagttcgtg 
      ccgggcaag gccgagatc aatcaagta cacatagaa 
      aacgaacca aattgggac gatgaagat aacattgtc 
      aataacttg acttgcctt tgcgttgtc ggaattgag 
      gaccctgta cgtcccgaa gtacctgac gccatcagg 
      aagtgtcag aaggccggg attacggtt cgaatggtc 
      accggtgat aatcttaac acagccagg tctatcgca 
      accaaatgc ggtatcgtc aaacctaac gaagatttc 
      ctcattatc gagggcaaa gaattcaac agacgcatt 
      cgagacagt actggagaa gtccaacaa catctactt 
      gacaaagta tggcctaaa ctacgtgta cttgcacgt 
      tcttctccc actgacaaa tacacctta gtcaaaggt 
      ataatcgac agcaaagtt aacgaaaat cgtgaagtg 
      gtcgccgta actggtgac ggcacaaac gatggtcct 
      gcgttgaaa aaggccgac gttggtttc gccatgggt 
      atcgctggc acagacgtg gcaaaagaa gcctctgat 
      attattttg actgatgac aactttagc agtatcgtc 
      aaggccgtg atgtgggga cgtaacgtt tacgacagt 
      atagcaaag tttctgcag tttcagctt accgttaac 
      gttgtagct gttgttgta gcatttatt ggtgcctgt 
      gctgttcaa gacagtcct ttgaaggct gtccaaatg 
      ctgtgggtt aacttaatt atggatact ttagcttct 
      ctcgcttta gctacagaa cttcccaca aacgatttg 
      ttgttgaga aagccgtac ggcaggact aaacctttg 
      atttcacgg acgatgatg aagaatatt cttggacaa 
      gcagtttac cagttaact gtaattttt gctcttctt 
      tttgtcggg gacaagttg ttagatatt gaatctgga 
      cgtggaacc gacctcggt gccggacct acccaacat 
      tttaccgtt atctttaat tcttttgta atgatgact 
      ctcttcaat gagttcaat gcgaggaaa atccacgga 
      cagcgcaac gtatttgag gggatcttc accaatcca 
      attttctat acaatttgg attggcacg tgtgtgtca 
      caaattctt atcattcag tatggtaag atggctttt 
      gccactaaa agcctgacc ttggagcag tggctctgg 
      tgcctcttc ttcggttta ggaacgttg ctttgggga 
      caacttgtt actacagtt cctactcgt aaaataccc 
      aagattctt tcttggggt cgcggccat cccgaggag 
      tacactgaa gcaattgcc attggcgaa gagaagttc 
      gacgtagat tcagacaag aagcccaga gctggccag 
      atcttgtgg atccgcggt ttgactagg ttgcaaacg 
      cagctgcga gtaatcaga gctttcaag tccaccctg 
      gaggatctg gaggagcgt cgctcggta catagttta 
      cacagctta cacagtttg cgcagctcg cgaagccac 
      accggccct tggccgcct cgtcctctc tcagacatc 
      acttacata gacgaggac ccaaccgcc aacaaattg 
      tcgccgcaa ccgtcgaag aacgagcgc gacgaccac 
      cggctgcta tctcccaac accttgaag ctcccaccc 
      cataacccc caatacaat gcgcagcac ctggccccc 
      tcctccgcg aacaactcg gatttgccc aagcccgtg 
      catgagact cgcatctag
    mRNA sequence
    • >TC001467 RA
      acctgagta aaaatcccg atcggcaat tgtttgtag 
      tgtttgttt gtgtgtgaa taattgcag ttttaggag 
      ctttctcgt aattaatag ccttaatta agcgttttt 
      ctgatctag ctgtgagaa atggctacg atagacggc 
      cgtcccgcg caatatgga attaccctg aaacagtta 
      cgcgacctc atggaacat cgaggtcgc gaaggagta 
      aataaaatt gctgacttt ggaggagta caagaaatt 
      tgtaagaaa ttatacaca tcgcccagt gaaggtctt 
      agcgggtca caggtggac cttgaacat agaagagaa 
      acattcgga tcaaactca attcctccc aaacctcca 
      aaaactttt cttcaatta gtatgggaa gctttacaa 
      gacatcact cttattatt ctggaagta gcagccatt 
      gtgtcttta ggtctttct ttctatcag ccgcaacaa 
      gaagatgtc ccttttgac gatgatgaa actagtcat 
      ggttggatt gagggttta gctatttta atctccgtt 
      atcgtagta gtcttagta acagcattt aacgattat 
      acgaaagaa agacaattc agaggtctt cagagtcga 
      atcgaggga gaacataaa tttgctgtg attcgacaa 
      gctgaagta aaacaagtt tccgttagc gacatagtt 
      gtaggtgat atttgccag ataaaatac ggtgatctt 
      ttaccggca gacggcatc ctaatccaa tccaatgac 
      ctcaaggtg gacgaatct tctcttacg ggcgagtca 
      gaccatgtc aaaaagggc gaaaactac gaccctatg 
      gtcttgtct ggcacccac gtgatggaa ggttcagga 
      aaaatgttg gttactgcg gttggtgtc aactcccaa 
      gcaggtatc atcttcaca ctactagga gcagcagtt 
      gatgaacag gaagccgaa attaagaaa atgaagaaa 
      gaagctaaa aagcagcgg aagaagaaa agtctaaca 
      ggtgctgac gatgaaaac gtaactggt aacagtcat 
      atgaattct cccgctccg gttccaaat aagcttaac 
      gagagtaaa caagaatcc aaagaaaat cacgtatcg 
      tcaccaccg gcgtcggcg gaaagtcac aagaaagaa 
      aagtcggtt cttcaagca aaattgacg aaacttgcc 
      attcagatt ggatatgcc ggttctaca attgccgtt 
      ctcactgtt gtaattttg ataattcag ttttgcgtt 
      aaaacctac gttgttgag ggcaattcg tggcaaaag 
      aatcacgcc agccacttg gtgcgtcat ttgatcatc 
      ggtgtaact gtactcgtg gtggcagta ccagaaggt 
      ttgccgctc gctgttacg ctttcttta gcttatagt 
      gtcaagaaa atgatgaaa gataacaat ttggtaaga 
      catttggac gcttgcgaa accatgggt aatgccact 
      gcaatttgt tcggacaaa accggaact ttgaccacc 
      aatcgcatg accgttgtg caatcctac atttgtgag 
      cagttgtgt aaatccatg ccgaaattt tctgatatt 
      cctgcacat gtcggaaat gcgatcctc cagggcatt 
      gcggttaat tgcgcttac acatcgcga gttatgcct 
      ccggatgat ccgaccgac ttgcccaag caagttggt 
      aacaaaact gagtgcgca ttgttggga ttcgtcctc 
      ggtctcggc aagaactac caaacgatc cgcgatgac 
      tatcctgag gagagtttt acccgcgtt tatacattt 
      aactctgtg agaaaatcc atgagcact gtcatcccc 
      agagcgggt ggtggatat cgattgtac acaaaaggt 
      gcttctgag atgatttta aacaagtgt gccttcatt 
      tacggtcac gacggccgt ttagaaaag ttcaccaga 
      gatatgcaa gagcgtttg ttgaagcaa gttatcgaa 
      ccaatggct tgtgatggt cttcggacg atctgtatc 
      gctttccgc gagttcgtg ccgggcaag gccgagatc 
      aatcaagta cacatagaa aacgaacca aattgggac 
      gatgaagat aacattgtc aataacttg acttgcctt 
      tgcgttgtc ggaattgag gaccctgta cgtcccgaa 
      gtacctgac gccatcagg aagtgtcag aaggccggg 
      attacggtt cgaatggtc accggtgat aatcttaac 
      acagccagg tctatcgca accaaatgc ggtatcgtc 
      aaacctaac gaagatttc ctcattatc gagggcaaa 
      gaattcaac agacgcatt cgagacagt actggagaa 
      gtccaacaa catctactt gacaaagta tggcctaaa 
      ctacgtgta cttgcacgt tcttctccc actgacaaa 
      tacacctta gtcaaaggt ataatcgac agcaaagtt 
      aacgaaaat cgtgaagtg gtcgccgta actggtgac 
      ggcacaaac gatggtcct gcgttgaaa aaggccgac 
      gttggtttc gccatgggt atcgctggc acagacgtg 
      gcaaaagaa gcctctgat attattttg actgatgac 
      aactttagc agtatcgtc aaggccgtg atgtgggga 
      cgtaacgtt tacgacagt atagcaaag tttctgcag 
      tttcagctt accgttaac gttgtagct gttgttgta 
      gcatttatt ggtgcctgt gctgttcaa gacagtcct 
      ttgaaggct gtccaaatg ctgtgggtt aacttaatt 
      atggatact ttagcttct ctcgcttta gctacagaa 
      cttcccaca aacgatttg ttgttgaga aagccgtac 
      ggcaggact aaacctttg atttcacgg acgatgatg 
      aagaatatt cttggacaa gcagtttac cagttaact 
      gtaattttt gctcttctt tttgtcggg gacaagttg 
      ttagatatt gaatctgga cgtggaacc gacctcggt 
      gccggacct acccaacat tttaccgtt atctttaat 
      tcttttgta atgatgact ctcttcaat gagttcaat 
      gcgaggaaa atccacgga cagcgcaac gtatttgag 
      gggatcttc accaatcca attttctat acaatttgg 
      attggcacg tgtgtgtca caaattctt atcattcag 
      tatggtaag atggctttt gccactaaa agcctgacc 
      ttggagcag tggctctgg tgcctcttc ttcggttta 
      ggaacgttg ctttgggga caacttgtt actacagtt 
      cctactcgt aaaataccc aagattctt tcttggggt 
      cgcggccat cccgaggag tacactgaa gcaattgcc 
      attggcgaa gagaagttc gacgtagat tcagacaag 
      aagcccaga gctggccag atcttgtgg atccgcggt 
      ttgactagg ttgcaaacg cagctgcga gtaatcaga 
      gctttcaag tccaccctg gaggatctg gaggagcgt 
      cgctcggta catagttta cacagctta cacagtttg 
      cgcagctcg cgaagccac accggccct tggccgcct 
      cgtcctctc tcagacatc acttacata gacgaggac 
      ccaaccgcc aacaaattg tcgccgcaa ccgtcgaag 
      aacgagcgc gacgaccac cggctgcta tctcccaac 
      accttgaag ctcccaccc cataacccc caatacaat 
      gcgcagcac ctggccccc tcctccgcg aacaactcg 
      gatttgccc aagcccgtg catgagact cgcatctag 
      tgccggtcc tggccgcag agccgcggc gcgcaccgc 
      atgcatcat atccgtttc cggtgtagc ttgccgttg 
      ccgttgccg ttgccgcca tagcgtagt cgctctgcc 
      tctgctgct ctgctctct tgaccgctc cagcgtccc 
      acccccgac acttaccac cacccccac caaccacta 
      ccgccctgg atccatcac caatcagac gattttatt 
      atctagtag tagatgttg cctaccact attgccatc 
      cttgttatt attataacc ctatactac ccgactatc 
      cttctatcg tttagctta gcactaagt agacttttt 
      cttacttga ccaaatctg agtattttg tattatttt 
      gtacataat ttcgtcgtt cactgtaga ctagctgta 
      tgtatagca ttttagata gccttagac ctcaggtta 
      gtggtaatg gaaacaaat attttattt ttgaacttg 
      ttgaacgca tgcacacaa ctgcgagtc atctgtatt 
      gtgtgtcga ccgccaacg acatgatca gtgttaata 
      atacataaa aaaacaagt caaacgaaa aacgatcat 
      gcgaaacga atgataaat tacatcaat ttttattta 
      ttattattt ttgtattcg gtcagcagg ccacattac 
      ttaatactc aattatcta atttataag atgttacat 
      ttgtatatt gatggttaa aatttcctg ttcattttt 
      tacgtcgaa tataacgtt tttttatac tatatagcg 
      atgtttcta tgctctgtt cttgatatt cggcactgg 
      gccacatgt agatacatg tttcgagca atcgtgtgt 
      ctgttgatt gtaacactt ttatttaca gcacagcga 
      acatgcgga agtcgtgcc cttttctca aagggcaca 
      ttgcagcgg gcttccgta tgctcttta caggtctcg 
      gcgaagggc gtccgtaaa tcaccatga tgatcatta 
      ttattatta tcattaggt attattatt cgtttgaca 
      gatgtccgg agaagtaag taagggcgt tcggatcgg 
      agatgtcgt ccttcgccg gcgcctgcc actcttctc 
      aggttacta gtagtatct gattctatc ttgttctgt 
      gcactatct attgtatgt gtctgtcac tgtctatcg 
      aaccactct taacgttgc cctttactc ttgaaggtg 
      ataggtggt gaattgcaa gaacgtttg attccggtt 
      ccgtacagc aagagctcc actgaccaa gctgtaagc 
      cgctttcct acatctttc tgtccgcta tgtattcgt 
      atgccgctg ttcgctctt gttagtttg tgtttggct 
      acgcttcca ctgcttcca cattgtgta gaggcacaa 
      gctttcatt accacgact gatcgatct atgcttgcc 
      tctaaacgt cttatttta gcgatttca gttccaaat 
      ttcaacgtt aacgaacgc cccttatca atatcgaag 
      cagtgaagt tttttttta ataattact aatgttact 
      attggtttt tatgtagac atttttccg tatttcttt 
      gtctgctga ttggttttt tattaactt ttttatgtt 
      tttattcgc atggtgcat gtttattac aatttttat 
      tatgttgtt ttcgtttat taattatta aatttattt 
      acacctagt tcacagatg cataagatc gctcaaaac 
      gactgctcg gccgacttt tgtttttgt tttattttt 
      tcgttcatt aaattttac tcaagtta