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Gene information
General information
TC001458 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3873948..3876189
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001458
    iBeetle screen iB_00252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001458 RA
      MDGPRPEAY LEEPTGERW DPLGAPNDQ ECGDKLELD 
      YCDNQLTYE KLRNLEEEQ ELLNSSLFA LTTHFAQVQ 
      FRLRQVVNA PLEEREDLL KSLEEFAFR GIPDISLVK 
      ERMDEASLA EAVRLRRSQ QKELIDRLK SQLRELEQY 
      AFENGEAGV PQDIVLERQ RVILNELKT RMNLEIDER 
      NYHQMTSAD VKQQINIAL DQLISPLRV KEHLVAQLK 
      TQVADLERF INYLQSDTK HDKCSCGCA YHSLKKPFK 
      SDTIGLIQR TATLLQMFA VLQLGCGAH SFRKNDLKN 
      SMKINHWGD LRAKLEMAI FRIRELLNE EQIDYSSDP 
      ESVAYNSQL TTAVRKYLA TSIRDLMQH GATSFVNSS 
      SLVPFIGCF PRRNSASSA HIHAWEIIL EYYHLKNGE 
      KFNSTPARK LSQSFNLDI AGASPTSNK HNMLCTIGN 
      IISTHTPYK RSYDSHFKA FICAALNSN KLVSWLSLI 
      FQCSQIVKL HYFPWSYVA KTGFQDSLK SLDSLTGYK 
      FDLPVDLAV RQFQNIKDV FT
    CDS sequence
    • >TC001458 RA
      atggacggg ccaagacct gaggcatac ttggaggag 
      ccaaccggc gaacgctgg gaccccctt ggtgctccc 
      aacgaccaa gaatgcggc gataaactc gaacttgac 
      tattgtgat aaccaacta acgtatgaa aaattgcgg 
      aacctggag gaggaacaa gagttgctt aattcgtcg 
      ctgtttgca ctaaccacg cattttgcc caggtccag 
      ttccgcctc cgccaagtc gtaaatgcc ccccttgag 
      gagagagaa gacttgctt aagtcttta gaagagttt 
      gcgtttcgg ggaatcccc gacattagt ctagtcaag 
      gagcgcatg gacgaggcc agtctggcc gaagccgtg 
      cgtttacgg cgctcgcaa caaaaggaa ttaatcgat 
      cggctgaaa tcccagcta agggaacta gaacaatac 
      gcgttcgag aacggagaa gccggggtt ccccaagac 
      attgtcctc gagcgccaa agagtcatt ctaaacgaa 
      ctgaaaacg cgaatgaac ctcgaaatc gacgagcgg 
      aactaccac cagatgact tcggccgac gttaagcag 
      caaatcaac atcgcgctg gaccagctt ataagcccg 
      ctccgggtg aaggagcac ctagtggcc cagttgaag 
      acgcaagtg gccgatctg gagcgcttc ataaactat 
      ttgcaaagt gacacgaaa cacgataag tgctcatgc 
      ggatgcgct tatcactcg ctgaaaaag ccgttcaaa 
      tcggacacc atagggtta atccaacga actgccacg 
      ttactgcaa atgttcgcc gtgctgcag ctgggctgc 
      ggggcgcac tctttcagg aaaaacgat ctcaaaaat 
      agcatgaaa atcaatcac tggggcgac ttgagggcg 
      aagctcgag atggcgatt ttccgcatc cgcgagctg 
      ttgaacgag gagcagatt gactacagt agtgacccc 
      gagtcggtg gcgtacaac agtcagttg acgaccgct 
      gtgcggaaa tacctagcc acgagcata cgggatttg 
      atgcaacac ggggcgact tcgtttgta aatagttcg 
      agtctggtc ccttttatc gggtgtttt ccgcggcgg 
      aattcggcc tcgagtgct cacatacac gcctgggag 
      attatcctc gagtattac catttgaaa aacggcgaa 
      aagttcaat tcgacccca gcccggaag ttgtcgcaa 
      agcttcaat ttggatatt gctggggct tcgcccact 
      agcaataaa cacaatatg ctttgtacg atcgggaat 
      atcatctcg actcatacg ccgtataag aggagttac 
      gattcacac ttcaaggcg ttcatctgc gcggcgcta 
      aactcgaat aagctggtc tcttggttg agcctaatt 
      tttcagtgt agtcaaatt gttaagttg cactatttc 
      ccgtggagc tacgttgca aaaactggg tttcaagac 
      agtctgaaa agtttagat tctttaact ggttacaag 
      tttgatcta ccagtcgat ctcgctgtg cgacagttc 
      caaaacata aaagacgta ttcacctaa
    mRNA sequence
    • >TC001458 RA
      cgtgacgtc agaggaaaa atgcgtttg cgtcccggc 
      gctgatgac gtcaattgt cgtaatgac agctgtcat 
      tgcggaaat gttggttgt gatttaacg ataattacg 
      ggtatgttg ttatcataa cagatattt taatgggtt 
      tagtggcat ggtttttgt aatatcgcc atgacacag 
      ttaaataac cccaaattg taagaagcg gctccgaac 
      atatgcgta atgtattaa agtaaatat tatggacgg 
      gccaagacc tgaggcata cttggagga gccaaccgg 
      cgaacgctg ggaccccct tggtgctcc caacgacca 
      agaatgcgg cgataaact cgaacttga ctattgtga 
      taaccaact aacgtatga aaaattgcg gaacctgga 
      ggaggaaca agagttgct taattcgtc gctgtttgc 
      actaaccac gcattttgc ccaggtcca gttccgcct 
      ccgccaagt cgtaaatgc cccccttga ggagagaga 
      agacttgct taagtcttt agaagagtt tgcgtttcg 
      gggaatccc cgacattag tctagtcaa ggagcgcat 
      ggacgaggc cagtctggc cgaagccgt gcgtttacg 
      gcgctcgca acaaaagga attaatcga tcggctgaa 
      atcccagct aagggaact agaacaata cgcgttcga 
      gaacggaga agccggggt tccccaaga cattgtcct 
      cgagcgcca aagagtcat tctaaacga actgaaaac 
      gcgaatgaa cctcgaaat cgacgagcg gaactacca 
      ccagatgac ttcggccga cgttaagca gcaaatcaa 
      catcgcgct ggaccagct tataagccc gctccgggt 
      gaaggagca cctagtggc ccagttgaa gacgcaagt 
      ggccgatct ggagcgctt cataaacta tttgcaaag 
      tgacacgaa acacgataa gtgctcatg cggatgcgc 
      ttatcactc gctgaaaaa gccgttcaa atcggacac 
      catagggtt aatccaacg aactgccac gttactgca 
      aatgttcgc cgtgctgca gctgggctg cggggcgca 
      ctctttcag gaaaaacga tctcaaaaa tagcatgaa 
      aatcaatca ctggggcga cttgagggc gaagctcga 
      gatggcgat tttccgcat ccgcgagct gttgaacga 
      ggagcagat tgactacag tagtgaccc cgagtcggt 
      ggcgtacaa cagtcagtt gacgaccgc tgtgcggaa 
      atacctagc cacgagcat acgggattt gatgcaaca 
      cggggcgac ttcgtttgt aaatagttc gagtctggt 
      cccttttat cgggtgttt tccgcggcg gaattcggc 
      ctcgagtgc tcacataca cgcctggga gattatcct 
      cgagtatta ccatttgaa aaacggcga aaagttcaa 
      ttcgacccc agcccggaa gttgtcgca aagcttcaa 
      tttggatat tgctggggc ttcgcccac tagcaataa 
      acacaatat gctttgtac gatcgggaa tatcatctc 
      gactcatac gccgtataa gaggagtta cgattcaca 
      cttcaaggc gttcatctg cgcggcgct aaactcgaa 
      taagctggt ctcttggtt gagcctaat ttttcagtg 
      tagtcaaat tgttaagtt gcactattt cccgtggag 
      ctacgttgc aaaaactgg gtttcaaga cagtctgaa 
      aagtttaga ttctttaac tggttacaa gtttgatct 
      accagtcga tctcgctgt gcgacagtt ccaaaacat 
      aaaagacgt attcaccta actacttaa ctagtcatt 
      ttttaatgt tattgtaca tttttgtta agtattact 
      agacttaga ccttcactt tgttaattg ctgtgtcta 
      taactatga atgtttatt tgtgtttgc cgaattaaa 
      taaattatt tgacaggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY